Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I207 R1
|
218 |
45.0 |
2482936 |
97.1% |
2410930 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,122,270 |
(A)6→5 |
coding (9/924 nt) |
yceM → |
Gfo/Idh/MocA family oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,122,265 | 0 | A | . | 100.0%
| 87.7
/ NA
| 21 | coding (4/924 nt) | yceM | Gfo/Idh/MocA family oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (8/13); total (8/13) |
GCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA > NZ_CP009273/1122184‑1122349
|
gcgTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaattac > 2:479798/1‑86 (MQ=255)
gcgTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaattac > 2:619094/1‑86 (MQ=255)
aGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCgg < 2:756406/90‑1 (MQ=255)
tGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAg > 2:940533/1‑90 (MQ=255)
ggAAATTGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGt < 1:130388/90‑1 (MQ=255)
ggAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGt < 1:553990/90‑1 (MQ=255)
ggAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGt < 1:940533/90‑1 (MQ=255)
ggAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGt < 2:184792/90‑1 (MQ=255)
tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGc < 2:95630/80‑1 (MQ=255)
tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGc > 1:95630/1‑80 (MQ=255)
tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt < 2:1211936/90‑1 (MQ=255)
gTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGt < 2:65146/90‑1 (MQ=255)
aCTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTgc < 2:864016/90‑1 (MQ=255)
aaaCAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCaa > 1:350069/1‑90 (MQ=255)
gCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGc > 2:447756/1‑90 (MQ=255)
gTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGt < 1:1155368/90‑1 (MQ=255)
ttCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTAcc < 1:1771/90‑1 (MQ=255)
aTCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAg > 2:429792/1‑90 (MQ=255)
aTCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAg > 2:784546/1‑90 (MQ=255)
ttAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTg > 2:700143/1‑90 (MQ=255)
ttAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTg > 2:916449/1‑90 (MQ=255)
tAAAGTG‑AAAAATTACGTATCGGCGTAGT‑GGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGa < 1:1051551/90‑1 (MQ=255)
aaaGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGa < 1:619094/90‑1 (MQ=255)
|
GCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA > NZ_CP009273/1122184‑1122349
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGT > NZ_CP009273/1122192‑1122367
|
GCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAT > SRR3722089.96818/1‑100 (MQ=60)
GGAAATTGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGT < SRR3722089.131977/100‑1 (MQ=60)
GGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGT < SRR3722089.560423/100‑1 (MQ=60)
GGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGT < SRR3722089.954304/100‑1 (MQ=60)
GGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAA > SRR3722089.354414/1‑100 (MQ=60)
GTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGT < SRR3722089.1172950/100‑1 (MQ=60)
TTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCG < SRR3722089.1788/100‑1 (MQ=60)
TAAAGTG‑AAAAATTACGTATCGGCGTAGT‑GGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG < SRR3722089.1067315/100‑1 (MQ=60)
AAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG < SRR3722089.626866/100‑1 (MQ=60)
aaaatgAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG < SRR3722089.496418/94‑1 (MQ=60)
AAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCtgtctcttatacacatctgacgctgccgacgaccatcttagtgtagatctcgg > SRR3722089.576150/1‑47 (MQ=60)
AAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGT < SRR3722089.627411/100‑1 (MQ=60)
|
GCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGT > NZ_CP009273/1122192‑1122367
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
