Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I228 R1
|
214 |
26.5 |
1448192 |
97.1% |
1406194 |
86.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,122,270 |
(A)6→5 |
coding (9/924 nt) |
yceM → |
Gfo/Idh/MocA family oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,122,265 | 0 | A | . | 100.0%
| 41.8
/ NA
| 11 | coding (4/924 nt) | yceM | Gfo/Idh/MocA family oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (5/6); total (5/6) |
GGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA > NZ_CP009273/1122179‑1122349
|
ggCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGGGATTAAAGTGaaaa > 1:291181/1‑90 (MQ=255)
gCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGc < 1:78755/90‑1 (MQ=255)
cTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTa < 2:415536/90‑1 (MQ=255)
ggAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGAAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGt < 2:356595/90‑1 (MQ=255)
tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt > 2:703863/1‑90 (MQ=255)
gCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGc > 2:380247/1‑90 (MQ=255)
tCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt > 1:279216/1‑58 (MQ=255)
tCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt < 2:279216/58‑1 (MQ=255)
gTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCgg < 1:497403/45‑1 (MQ=255)
gTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCgg > 2:497403/1‑45 (MQ=255)
aTCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAg > 2:365376/1‑90 (MQ=255)
aaaGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGa < 1:236949/90‑1 (MQ=255)
|
GGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA > NZ_CP009273/1122179‑1122349
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG > NZ_CP009273/1122191‑1122359
|
attcgtcggcagcgtcagatgtgtataagagacagGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGA < SRR3722112.504608/65‑1 (MQ=60)
GCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAT < SRR3722112.79712/100‑1 (MQ=60)
GCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATctgtctcttatacacatctgac > SRR3722112.282461/1‑78 (MQ=60)
AAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG < SRR3722112.239777/100‑1 (MQ=60)
|
AGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGG > NZ_CP009273/1122191‑1122359
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
