Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I211 R1
|
223 |
18.0 |
1007644 |
96.3% |
970361 |
85.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,325,886 |
T→C |
G194G (GGT→GGC) |
topA → |
type I DNA topoisomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,325,886 | 0 | T | C | 100.0%
| 11.2
/ NA
| 5 | G194G (GGT→GGC) | topA | type I DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/1); total (4/1) |
ACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCG > NZ_CP009273/1325819‑1325971
|
aCCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTc < 2:102327/90‑1 (MQ=255)
ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtgagcg > 1:267461/1‑90 (MQ=255)
gTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGa > 1:98261/1‑90 (MQ=255)
gTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGa > 2:445276/1‑90 (MQ=255)
ccGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCg > 1:95076/1‑90 (MQ=255)
|
ACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCG > NZ_CP009273/1325819‑1325971
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCG > NZ_CP009273/1325787‑1325971
|
TGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGC < SRR3722094.30624/100‑1 (MQ=60)
GGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGGTCGCCTTGTGGTTGAGCGGGAG > SRR3722094.149497/1‑100 (MQ=60)
GGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCG > SRR3722094.271446/1‑100 (MQ=60)
CTCGTGGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGA > SRR3722094.99683/1‑100 (MQ=60)
GGCCTGTCTGCCGGCCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCG > SRR3722094.96458/1‑100 (MQ=60)
|
TGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCG > NZ_CP009273/1325787‑1325971
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
