Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I211 R1
|
223 |
18.0 |
1007644 |
96.3% |
970361 |
85.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,739,096 |
T→C |
E61G (GAA→GGA) |
rimM ← |
ribosome maturation factor RimM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,739,096 | 0 | T | C | 100.0%
| 17.7
/ NA
| 7 | E61G (GAA→GGA) | rimM | ribosome maturation factor RimM |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/3); total (4/3) |
TCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTT > NZ_CP009273/2739009‑2739162
|
tCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagcttccc > 2:308313/1‑90 (MQ=255)
aaTTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagcttcccagct < 1:148625/90‑1 (MQ=255)
cGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGAc < 2:44230/90‑1 (MQ=255)
ttCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCAc < 2:140053/90‑1 (MQ=255)
cAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATaa > 1:316385/1‑90 (MQ=255)
cAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAACTTCGCAGCTGGACTTGCGGCCGGTGACCCGCCTTCTGTATaa > 1:11063/1‑90 (MQ=255)
tCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCtt > 1:182411/1‑90 (MQ=255)
ccTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCttt < 1:314913/90‑1 (MQ=255)
ccTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCttt > 2:370372/1‑90 (MQ=255)
|
TCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTT > NZ_CP009273/2739009‑2739162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTC > NZ_CP009273/2739008‑2739172
|
TTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACT < SRR3722094.263633/100‑1 (MQ=60)
AATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTG < SRR3722094.150707/100‑1 (MQ=60)
TCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAA > SRR3722094.321414/1‑100 (MQ=60)
TCACGATAGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAACTTCGCAGCTGGACTTGCGGCCGGTGACCCGCCTTCTGTATAA > SRR3722094.11237/1‑100 (MQ=60)
GATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTT > SRR3722094.184911/1‑100 (MQ=60)
CCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTC < SRR3722094.319891/100‑1 (MQ=60)
|
TTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTC > NZ_CP009273/2739008‑2739172
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
