Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	433,738	A→C	13.4%	intergenic (‑147/+33)	yajO ← / ← dxs	2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	433,738	0	A	C	13.4%	603.2 / 104.5	246	intergenic (‑147/+33)	yajO/dxs	2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand): ref base A (118/95); new base C (18/15); total (136/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 259 total aligned reads displayed.

CATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCAG  >  CP009273/433589‑433887

 aTGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGaataa                                                                                                                                                      >  2:1615959/1‑150 (MQ=255)

 aTGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGaataa                                                                                                                                                      >  1:1414188/1‑150 (MQ=255)

     tgcCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTc                                                                                                                                                  >  1:1931340/1‑150 (MQ=255)

       ccACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCtt                                                                                                                                                <  1:2393980/150‑1 (MQ=255)

         aCTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTaa                                                                                                                                              >  1:4640056/1‑150 (MQ=255)

          cTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAg                                                                                                                                             <  1:3260291/150‑1 (MQ=255)

           tCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGc                                                                                                                                            <  1:1443941/150‑1 (MQ=255)

            ccTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGGATTATTTAGAGTCTATGAATAATTTCTTAAGCa                                                                                                                                           <  2:3934885/150‑1 (MQ=255)

             cTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCAt                                                                                                                                          <  1:3821888/150‑1 (MQ=255)

              tGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATa                                                                                                                                         <  1:2888151/150‑1 (MQ=255)

                 tataCTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCa                                                                                                                                      >  2:510196/1‑150 (MQ=255)

                  ataCTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAg                                                                                                                                     >  1:2086415/1‑150 (MQ=255)

                    aCTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAgga                                                                                                                                   >  1:210330/1‑150 (MQ=255)

                       cGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTTACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAggagtg                                                                                                                                <  2:1092957/150‑1 (MQ=255)

                       cGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAggagtg                                                                                                                                <  2:501922/150‑1 (MQ=255)

                         tCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAggagtgga                                                                                                                              >  1:4257752/1‑150 (MQ=255)

                          cATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAggagtggag                                                                                                                             >  1:644785/1‑150 (MQ=255)

                           aTACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAggagtggagt                                                                                                                            <  1:2987494/150‑1 (MQ=255)

                           aTACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAggagtggagt                                                                                                                            >  1:2649077/1‑150 (MQ=255)

                              cTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAAGTATTTCGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAgg                                                                                                                         >  1:522414/1‑150 (MQ=255)

                                  aaGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCACCTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGAtt                                                                                                                     >  2:2302751/1‑150 (MQ=255)

                                   aGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTa                                                                                                                    >  2:3547224/1‑150 (MQ=255)

                                    gTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTAt                                                                                                                   <  2:282452/150‑1 (MQ=255)

                                     ttGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATg                                                                                                                  >  2:658248/1‑150 (MQ=255)

                                       gCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgcc                                                                                                                >  2:89480/1‑150 (MQ=255)

                                       gCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgcc                                                                                                                <  2:721467/150‑1 (MQ=255)

                                             gctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGccagccag                                                                                                          <  2:1914677/150‑1 (MQ=255)

                                              ctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAgg                                                                                                         >  2:3222552/1‑150 (MQ=255)

                                              ctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAgg                                                                                                         <  2:4534451/150‑1 (MQ=255)

                                                 cGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCt                                                                                                      >  1:3910063/1‑150 (MQ=255)

                                                  ggCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCtt                                                                                                     >  1:2449338/1‑150 (MQ=255)

                                                    cTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGa                                                                                                   <  2:3691838/150‑1 (MQ=255)

                                                     tGCATTCGTTCACCCCAGTCACTTAATTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTATTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAt                                                                                                  <  1:1840890/150‑1 (MQ=255)

                                                      gCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAtt                                                                                                 <  2:3727742/150‑1 (MQ=255)

                                                        aTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAtttt                                                                                               >  2:2245652/1‑150 (MQ=255)

                                                         ttCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTg                                                                                              <  2:1732641/150‑1 (MQ=255)

                                                         ttCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTg                                                                                              >  1:924595/1‑150 (MQ=255)

                                                            gTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCt                                                                                           >  2:259376/1‑150 (MQ=255)

                                                             ttCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCtt                                                                                          >  1:2471406/1‑150 (MQ=255)

                                                               cACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTcc                                                                                        <  2:3091827/150‑1 (MQ=255)

                                                               cACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTTGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTcc                                                                                        <  1:2536275/150‑1 (MQ=255)

                                                                  cccAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATa                                                                                     <  1:3505572/150‑1 (MQ=255)

                                                                  cccAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATa                                                                                     <  2:2028878/150‑1 (MQ=255)

                                                                               ttaTGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGa                                                                        >  2:2494914/1‑150 (MQ=255)

                                                                                taTGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAg                                                                       <  1:867943/150‑1 (MQ=255)

                                                                                     aaGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGa                                                                  <  1:4098392/150‑1 (MQ=255)

                                                                                         tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                              >  1:2151205/1‑150 (MQ=255)

                                                                                         tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                              <  2:4213526/150‑1 (MQ=255)

                                                                                         tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                              <  2:1335884/150‑1 (MQ=255)

                                                                                         tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                              >  1:1299377/1‑150 (MQ=255)

                                                                                          ccTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCg                                                             >  2:3544285/1‑150 (MQ=255)

                                                                                          ccTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCg                                                             <  1:3185106/150‑1 (MQ=255)

                                                                                           cTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCgg                                                            <  1:4484660/150‑1 (MQ=255)

                                                                                             ggggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcg                                                          <  2:3052244/150‑1 (MQ=255)

                                                                                             ggggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcg                                                          <  1:3109254/150‑1 (MQ=255)

                                                                                              gggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcgc                                                         <  1:1777945/150‑1 (MQ=255)

                                                                                               ggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcgcg                                                        >  2:508907/1‑150 (MQ=255)

                                                                                               ggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcgcg                                                        <  2:1007928/150‑1 (MQ=255)

                                                                                               ggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcgcg                                                        <  2:387063/150‑1 (MQ=255)

                                                                                                  ttCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCAt                                                     >  1:3313721/1‑150 (MQ=255)

                                                                                                  ttCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCAt                                                     >  1:563819/1‑150 (MQ=255)

                                                                                                   tCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCAtt                                                    >  2:1657272/1‑150 (MQ=255)

                                                                                                      cTCGTTTGCCGCCTTCCTGCAACTCGAATTNTTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATttct                                                 >  1:4284423/1‑150 (MQ=255)

                                                                                                        cGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATttcttc                                               >  1:552602/1‑150 (MQ=255)

                                                                                                           ttGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTg                                            >  2:4540602/1‑150 (MQ=255)

                                                                                                           gtGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATATCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTg                                            <  2:4311458/149‑1 (MQ=255)

                                                                                                            tGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGa                                           >  1:1271117/1‑150 (MQ=255)

                                                                                                            tGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGa                                           <  2:2962839/150‑1 (MQ=255)

                                                                                                             gccgccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAg                                          <  1:1704111/150‑1 (MQ=255)

                                                                                                                gccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTc                                       <  2:4093574/150‑1 (MQ=255)

                                                                                                                gccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTc                                       <  2:4093307/150‑1 (MQ=255)

                                                                                                                 ccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTcc                                      <  1:2551076/150‑1 (MQ=255)

                                                                                                                  cTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCt                                     <  1:1084036/150‑1 (MQ=255)

                                                                                                                  cTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCt                                     >  1:4433954/1‑150 (MQ=255)

                                                                                                                   ttCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCtt                                    <  2:227017/150‑1 (MQ=255)

                                                                                                                    tCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTg                                   <  1:2867827/150‑1 (MQ=255)

                                                                                                                     ccTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGc                                  >  1:3397374/1‑150 (MQ=255)

                                                                                                                       tGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCgg                                <  1:1561221/150‑1 (MQ=255)

                                                                                                                        gCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGa                               >  2:1538325/1‑150 (MQ=255)

                                                                                                                        gCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGa                               <  1:2680478/150‑1 (MQ=255)

                                                                                                                        gCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGa                               <  1:362641/150‑1 (MQ=255)

                                                                                                                              cGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaag                         >  2:3488215/1‑150 (MQ=255)

                                                                                                                              cGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaag                         >  1:2950374/1‑150 (MQ=255)

                                                                                                                                 attattTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaagaag                      <  1:3973718/150‑1 (MQ=255)

                                                                                                                                 attattTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaagaag                      <  2:219346/150‑1 (MQ=255)

                                                                                                                                   tattTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTc                    >  1:2021276/1‑150 (MQ=255)

                                                                                                                                   tattTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATAACAGCGGCATCGAGGCCGAGATCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTc                    >  1:4555426/1‑150 (MQ=255)

                                                                                                                                    attTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTcc                   >  2:1438858/1‑150 (MQ=255)

                                                                                                                                    attTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTcc                   >  1:2071782/1‑150 (MQ=255)

                                                                                                                                     tttAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCg                  <  1:3460803/150‑1 (MQ=255)

                                                                                                                                     tttAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCg                  <  1:739816/150‑1 (MQ=255)

                                                                                                                                        agagTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCa               <  2:4384425/150‑1 (MQ=255)

                                                                                                                                           gTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGc            >  2:3929498/1‑150 (MQ=255)

                                                                                                                                            tCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGcc           >  1:3585765/1‑150 (MQ=255)

                                                                                                                                               aTGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAAt        <  2:1157133/150‑1 (MQ=255)

                                                                                                                                               aTGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAAt        <  1:1035115/150‑1 (MQ=255)

                                                                                                                                                 gAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGt      >  1:2639296/1‑150 (MQ=255)

                                                                                                                                                 gAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGt      >  2:3338949/1‑150 (MQ=255)

                                                                                                                                                  aataatTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGtt     <  1:552444/150‑1 (MQ=255)

                                                                                                                                                    taatTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCa   <  2:2916631/150‑1 (MQ=255)

CATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCAG  >  CP009273/433589‑433887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A