Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	433,738	A→C	48.4%	intergenic (‑147/+33)	yajO ← / ← dxs	2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	433,738	0	A	C	48.4%	68.0 / inf	261	intergenic (‑147/+33)	yajO/dxs	2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand): ref base A (82/53); new base C (68/58); total (150/111)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 272 total aligned reads displayed.

ATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCA  >  CP009273/433590‑433886

aTGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAAGTATTTAGAGTCTATGaataa                                                                                                                                                     >  2:2421630/1‑150 (MQ=255)

   ctgcCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCAttt                                                                                                                                                  <  1:3721419/150‑1 (MQ=255)

   ctgcCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCAttt                                                                                                                                                  >  2:2355209/1‑150 (MQ=255)

    tgcCCCTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCTTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTc                                                                                                                                                 <  2:201401/150‑1 (MQ=255)

    tgcCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTc                                                                                                                                                 >  2:2484750/1‑150 (MQ=255)

      ccACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATTAATCATTTCtt                                                                                                                                               <  2:2384180/150‑1 (MQ=255)

      ccACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCtt                                                                                                                                               >  2:351243/1‑150 (MQ=255)

          tCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGc                                                                                                                                           >  2:2894394/1‑150 (MQ=255)

          tCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGc                                                                                                                                           >  2:1675367/1‑150 (MQ=255)

          tCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGc                                                                                                                                           >  2:4076734/1‑150 (MQ=255)

             tGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATa                                                                                                                                        >  1:2577702/1‑150 (MQ=255)

             tGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATa                                                                                                                                        >  1:2682/1‑150 (MQ=255)

              gCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAg                                                                                                                                       <  2:1226915/150‑1 (MQ=255)

              gCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAg                                                                                                                                       <  2:341644/150‑1 (MQ=255)

              gCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAg                                                                                                                                       <  1:1367950/150‑1 (MQ=255)

               cTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGc                                                                                                                                      <  2:1211715/150‑1 (MQ=255)

                    cTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTAATTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAggag                                                                                                                                 >  2:3031534/1‑150 (MQ=255)

                      cGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAggagtg                                                                                                                               >  1:624794/1‑150 (MQ=255)

                       gTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAggagtgg                                                                                                                              >  1:2646812/1‑150 (MQ=255)

                        tCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAggagtgga                                                                                                                             >  2:2089661/1‑150 (MQ=255)

                            aCTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAg                                                                                                                         >  1:3061227/1‑150 (MQ=255)

                            aCTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAg                                                                                                                         >  2:3183915/1‑150 (MQ=255)

                               tCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGa                                                                                                                      >  1:2785571/1‑150 (MQ=255)

                               tCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGa                                                                                                                      <  1:2225820/150‑1 (MQ=255)

                                 aaGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGAtt                                                                                                                    >  1:158847/1‑150 (MQ=255)

                                  aGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTa                                                                                                                   <  2:3255220/150‑1 (MQ=255)

                                  aGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTa                                                                                                                   >  2:1792981/1‑150 (MQ=255)

                                  aGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTa                                                                                                                   >  2:3928307/1‑150 (MQ=255)

                                   gTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTAt                                                                                                                  >  2:2658006/1‑150 (MQ=255)

                                   gTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTAt                                                                                                                  >  2:3075911/1‑150 (MQ=255)

                                   gTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTAt                                                                                                                  <  2:1871680/150‑1 (MQ=255)

                                    ttGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATg                                                                                                                 <  2:2915496/150‑1 (MQ=255)

                                       cATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgcca                                                                                                              >  2:378834/1‑150 (MQ=255)

                                         tgtgCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagc                                                                                                            >  1:3184752/1‑150 (MQ=255)

                                         tgtgCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagc                                                                                                            >  1:1089195/1‑150 (MQ=255)

                                          gtgCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagcc                                                                                                           <  2:2646124/150‑1 (MQ=255)

                                           tgctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagcca                                                                                                          >  2:2829388/1‑150 (MQ=255)

                                           tgctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagcca                                                                                                          >  2:636967/1‑150 (MQ=255)

                                           tgctgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATgccagcca                                                                                                          <  1:985542/150‑1 (MQ=255)

                                              tgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGc                                                                                                       >  1:1025976/1‑150 (MQ=255)

                                              tgcGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGc                                                                                                       >  2:2247474/1‑150 (MQ=255)

                                                  gCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTg                                                                                                   <  1:2280065/150‑1 (MQ=255)

                                                   cTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGa                                                                                                  >  1:912871/1‑150 (MQ=255)

                                                     gCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAtt                                                                                                <  2:458485/150‑1 (MQ=255)

                                                      cATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCATGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAtgt                                                                                               >  2:3610999/1‑148 (MQ=255)

                                                      cATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGAttt                                                                                               >  1:1910735/1‑150 (MQ=255)

                                                           gTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCt                                                                                          <  1:2929505/150‑1 (MQ=255)

                                                                ccccAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCAt                                                                                     >  2:3389401/1‑150 (MQ=255)

                                                                   cAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATAcc                                                                                  >  1:3769461/1‑150 (MQ=255)

                                                                   cAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATAcc                                                                                  >  1:565069/1‑150 (MQ=255)

                                                                       cACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCg                                                                              >  1:2698784/1‑150 (MQ=255)

                                                                       cACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCg                                                                              >  1:3052937/1‑150 (MQ=255)

                                                                        acttacttATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCgg                                                                             <  2:691238/150‑1 (MQ=255)

                                                                            acttaTGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATc                                                                         <  2:3969888/150‑1 (MQ=255)

                                                                             cttaTGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCg                                                                        <  2:125975/150‑1 (MQ=255)

                                                                             cttaTGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCg                                                                        >  2:2547862/1‑150 (MQ=255)

                                                                                     aGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAg                                                                <  2:4035240/150‑1 (MQ=255)

                                                                                      gCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGt                                                               >  1:2423026/1‑150 (MQ=255)

                                                                                        tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                             <  1:232849/150‑1 (MQ=255)

                                                                                        tCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTc                                                             <  1:3928904/150‑1 (MQ=255)

                                                                                         ccTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAAATATTTAGAGTCTATGAATAATTACTTAAGCATACCAGGAGTTGAGTAGGGATTATTCCAGCCAGGCCTACATTTTGGCTTCCATACCAGCGGCATCGATGCCGAGTTCg                                                            >  2:703924/1‑150 (MQ=255)

                                                                                          cTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCgg                                                           <  1:127023/150‑1 (MQ=255)

                                                                                           tGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgc                                                          >  2:427982/1‑150 (MQ=255)

                                                                                            ggggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcg                                                         <  2:600212/150‑1 (MQ=255)

                                                                                             gggCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGgcgc                                                        >  1:1973136/1‑150 (MQ=255)

                                                                                                    aCTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCGGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATttc                                                 >  2:2501916/1‑150 (MQ=255)

                                                                                                    aCTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGATTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGATGCCGAGTTCGGCGCGCATttc                                                 >  1:3973513/1‑150 (MQ=255)

                                                                                                      tCGTTTGCCGCCTTCCTGCAACTCTAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATttctt                                               >  1:3317108/1‑150 (MQ=255)

                                                                                                       cGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATttcttc                                              <  2:1911343/150‑1 (MQ=255)

                                                                                                         tttGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCt                                            <  1:2732761/150‑1 (MQ=255)

                                                                                                         tttGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCt                                            <  2:3660469/150‑1 (MQ=255)

                                                                                                           agccgccTTCCTGCAACTCGATTTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGa                                          <  2:2137514/149‑1 (MQ=255)

                                                                                                              cgccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATGATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGtt                                       <  2:2666787/150‑1 (MQ=255)

                                                                                                              cgccTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGtt                                       >  1:4143191/1‑150 (MQ=255)

                                                                                                                 cTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCt                                    >  2:2083075/1‑150 (MQ=255)

                                                                                                                     cTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCg                                <  1:3839245/150‑1 (MQ=255)

                                                                                                                     cTGCAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCg                                <  1:1188296/150‑1 (MQ=255)

                                                                                                                        cAACTCGAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGaa                             >  2:87145/1‑150 (MQ=255)

                                                                                                                         aaCTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAAt                            <  1:870128/150‑1 (MQ=255)

                                                                                                                             cGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaag                        >  2:2944076/1‑150 (MQ=255)

                                                                                                                             cGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaag                        >  2:1464908/1‑150 (MQ=255)

                                                                                                                              gAATTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaaga                       >  1:259979/1‑150 (MQ=255)

                                                                                                                               aaTTATTTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaagaa                      >  2:916608/1‑150 (MQ=255)

                                                                                                                                attattTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaagaag                     >  1:792639/1‑150 (MQ=255)

                                                                                                                                attattTAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAaagaag                     >  1:792436/1‑150 (MQ=255)

                                                                                                                                  tattTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTc                   >  2:4114870/1‑150 (MQ=255)

                                                                                                                                   attTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTcc                  >  1:264278/1‑150 (MQ=255)

                                                                                                                                     ttAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCgg                >  1:3785935/1‑150 (MQ=255)

                                                                                                                                      tAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAATAAGTCCGGc               >  2:3915928/1‑150 (MQ=255)

                                                                                                                                      tAGAGTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGc               >  2:3941610/1‑150 (MQ=255)

                                                                                                                                       agagTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCa              >  1:3142870/1‑150 (MQ=255)

                                                                                                                                         agTCTATGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAgg            >  1:3113600/1‑150 (MQ=255)

                                                                                                                                         agTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAgg            >  1:2812441/1‑150 (MQ=255)

                                                                                                                                         agTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAgg            <  1:4023310/150‑1 (MQ=255)

                                                                                                                                            cTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCa         >  1:3536006/1‑150 (MQ=255)

                                                                                                                                            cTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCa         >  1:3631862/1‑150 (MQ=255)

                                                                                                                                             tATGAATCATTTCTTAATCATAGCAGGAGTGGAGTAGGGATTAGGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGACGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCaa        <  1:1471592/150‑1 (MQ=255)

                                                                                                                                              aTGAATCATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAAt       <  2:3525185/150‑1 (MQ=255)

                                                                                                                                                   taatTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCa  >  1:2127526/1‑150 (MQ=255)

                                                                                                                                                   taatTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCa  >  1:48043/1‑150 (MQ=255)

ATGCTGCCACTCCTGCTATACTCGTCATACTTCAAGTTGCATGTGCTGCGGCTGCATTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGCTTCACTCGTTTGCCGCCTTCCTGCAACTCGAATTATTTAGAGTCTATGAATAATTTCTTAAGCATAGCAGGAGTGGAGTAGGGATTATGCCAGCCAGGCCTTGATTTTGGCTTCCATACCAGCGGCATCGAGGCCGAGTTCGGCGCGCATTTCTTCCTGAGTTCCTTGCGGAATAAAGAAGTCCGGCAGGCCAATGTTCA  >  CP009273/433590‑433886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A