Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 479,244 C→A 100% A206S (GCC→TCC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273479,2440CA98.3% 810.6 / 4.7 239A206S (GCC→TCC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (105/130);  total (109/130)
Fisher's exact test for biased strand distribution p-value = 4.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

GATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGT  >  CP009273/479097‑479390
                                                                                                                                                   |                                                                                                                                                  
gATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAgg                                                                                                                                                  >  2:801907/1‑150 (MQ=255)
  ttCACTTTCAGCAGTATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTa                                                                                                                                                <  1:482503/150‑1 (MQ=255)
  ttCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTa                                                                                                                                                <  2:2595773/150‑1 (MQ=255)
    cACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAt                                                                                                                                              <  2:1410636/150‑1 (MQ=255)
     aCTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATg                                                                                                                                             <  2:1173160/150‑1 (MQ=255)
     aCTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATg                                                                                                                                             >  1:3849047/1‑150 (MQ=255)
      cTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGa                                                                                                                                            >  2:713121/1‑150 (MQ=255)
       tttCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAc                                                                                                                                           >  1:41530/1‑150 (MQ=255)
         tCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAt                                                                                                                                         >  2:2017551/1‑150 (MQ=255)
         tCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAACCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACaa                                                                                                                                         >  1:1433394/1‑149 (MQ=255)
           agcagGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCa                                                                                                                                       >  1:1211966/1‑150 (MQ=255)
             cagGATTTTGCCTAACTCTTCAGGAGAGGTCAGACGCGTCTGAGCACTAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAc                                                                                                                                     >  1:1530163/1‑150 (MQ=255)
              agGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAcc                                                                                                                                    <  1:2562657/150‑1 (MQ=255)
                 aTTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGc                                                                                                                                 >  1:4470508/1‑150 (MQ=255)
                  ttttGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCg                                                                                                                                <  2:387306/150‑1 (MQ=255)
                   tttGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGt                                                                                                                               <  1:2737689/150‑1 (MQ=255)
                     tGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTa                                                                                                                             <  1:2096366/150‑1 (MQ=255)
                       cctaaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGc                                                                                                                           >  1:597996/4‑150 (MQ=255)
                         taaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTg                                                                                                                         >  1:1092327/2‑150 (MQ=255)
                              ctTCAGTAGAGGTCAGACGCGTCGGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAtt                                                                                                                    <  2:449629/150‑1 (MQ=255)
                               ttCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAttt                                                                                                                   <  2:3937750/150‑1 (MQ=255)
                               ttCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAttt                                                                                                                   >  2:45522/1‑150 (MQ=255)
                                 cAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGt                                                                                                                 >  2:4073122/1‑150 (MQ=255)
                                  aGTAGAGGTCCGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt                                                                                                                >  2:2998719/1‑150 (MQ=255)
                                  aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt                                                                                                                >  2:2456102/1‑150 (MQ=255)
                                  aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGtt                                                                                                                >  2:3087370/1‑150 (MQ=255)
                                      gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGc                                                                                                            >  1:1722229/1‑150 (MQ=255)
                                         gTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCa                                                                                                         <  1:1777929/150‑1 (MQ=255)
                                          tCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAt                                                                                                        <  2:3897610/150‑1 (MQ=255)
                                           cAGACGCGTCTGAGCAATGATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt                                                                                                       <  1:3795132/150‑1 (MQ=255)
                                            aGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTc                                                                                                      <  2:1584303/150‑1 (MQ=255)
                                               cgcgTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCggg                                                                                                   <  2:2701590/150‑1 (MQ=255)
                                                  gTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                                >  2:4400080/1‑150 (MQ=255)
                                                  gTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc                                                                                                >  1:3973328/1‑150 (MQ=255)
                                                    cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCAt                                                                                              >  1:3181754/1‑150 (MQ=255)
                                                     tGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATc                                                                                             >  1:2315455/1‑150 (MQ=255)
                                                     tGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATc                                                                                             >  1:1545198/1‑150 (MQ=255)
                                                        gCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATACAg                                                                                          >  1:2703898/1‑150 (MQ=255)
                                                          aataatAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAt                                                                                        <  2:3394710/150‑1 (MQ=255)
                                                           ataataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATa                                                                                       <  2:1977937/150‑1 (MQ=255)
                                                           ataataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATa                                                                                       >  2:3993126/1‑150 (MQ=255)
                                                            taataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAc                                                                                      <  1:3609711/150‑1 (MQ=255)
                                                              ataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc                                                                                    >  2:2770354/1‑150 (MQ=255)
                                                                agagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCAt                                                                                  <  2:4219842/150‑1 (MQ=255)
                                                                  agGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                                <  1:2932073/150‑1 (MQ=255)
                                                                        tAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACt                                                                          <  1:225311/150‑1 (MQ=255)
                                                                        tAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACt                                                                          >  2:1820455/1‑150 (MQ=255)
                                                                            cTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGa                                                                      >  2:2025155/1‑150 (MQ=255)
                                                                              gTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAc                                                                    <  1:3384072/150‑1 (MQ=255)
                                                                               ttGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAcc                                                                   <  2:783841/150‑1 (MQ=255)
                                                                               ttGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAcc                                                                   <  2:2157607/150‑1 (MQ=255)
                                                                                tGGCCTTTCATCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCg                                                                  <  1:780178/150‑1 (MQ=255)
                                                                                tGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGGTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCg                                                                  <  2:717819/150‑1 (MQ=255)
                                                                                    cTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaaca                                                              <  1:4593295/150‑1 (MQ=255)
                                                                                     tttCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaa                                                             >  2:4346507/1‑150 (MQ=255)
                                                                                       tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACt                                                           >  2:2889565/1‑150 (MQ=255)
                                                                                        cACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTg                                                          <  2:3965839/150‑1 (MQ=255)
                                                                                           cggcggCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAAc                                                       <  2:3545260/150‑1 (MQ=255)
                                                                                           cggcggCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAAc                                                       <  2:1893802/150‑1 (MQ=255)
                                                                                               ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                   >  1:4208907/1‑150 (MQ=255)
                                                                                               ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                   >  1:2369852/1‑150 (MQ=255)
                                                                                               ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                   >  1:4208952/1‑150 (MQ=255)
                                                                                               ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                   >  1:2428273/1‑150 (MQ=255)
                                                                                                    accaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCAc                                              <  2:1500500/150‑1 (MQ=255)
                                                                                                        ccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGccc                                          <  2:653601/150‑1 (MQ=255)
                                                                                                        ccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGccc                                          <  1:1280417/150‑1 (MQ=255)
                                                                                                        ccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGccc                                          <  1:803420/150‑1 (MQ=255)
                                                                                                         cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACACCTGAACATCACCCACGCCCg                                         <  1:1002990/150‑1 (MQ=255)
                                                                                                         cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCg                                         >  1:3163881/1‑150 (MQ=255)
                                                                                                         cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCg                                         <  1:3205890/150‑1 (MQ=255)
                                                                                                          gAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGa                                        <  1:4382671/150‑1 (MQ=255)
                                                                                                          gAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGa                                        <  2:1080105/150‑1 (MQ=255)
                                                                                                           aGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGAc                                       >  2:3266884/1‑150 (MQ=255)
                                                                                                            gCTGACCCGCCGCAAACTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGATCTACTGAAATTCACCCACGCCCGACg                                      <  2:344426/150‑1 (MQ=255)
                                                                                                             cTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGt                                     >  2:3219714/1‑150 (MQ=255)
                                                                                                               gACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAc                                   <  2:4470508/150‑1 (MQ=255)
                                                                                                                  ccgccgCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGc                                <  1:1911663/150‑1 (MQ=255)
                                                                                                                   cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGACTTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                               <  1:509289/150‑1 (MQ=255)
                                                                                                                   cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                               <  1:2393388/150‑1 (MQ=255)
                                                                                                                   cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                               <  1:2826864/150‑1 (MQ=255)
                                                                                                                   cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                               <  1:4411412/150‑1 (MQ=255)
                                                                                                                   cgccgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCt                               <  1:1430672/150‑1 (MQ=255)
                                                                                                                        cAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATgg                          >  2:1009061/1‑150 (MQ=255)
                                                                                                                             tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCt                     >  1:3986375/1‑150 (MQ=255)
                                                                                                                             tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCt                     <  2:1722231/150‑1 (MQ=255)
                                                                                                                             tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTGAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCt                     <  2:2282418/150‑1 (MQ=255)
                                                                                                                               ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                   >  1:1922381/1‑150 (MQ=255)
                                                                                                                               ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                   <  2:3054460/150‑1 (MQ=255)
                                                                                                                               ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                   <  2:3667556/150‑1 (MQ=255)
                                                                                                                                  gTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat                <  1:1725573/150‑1 (MQ=255)
                                                                                                                                  gTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat                <  1:2998719/150‑1 (MQ=255)
                                                                                                                                   ttCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCata               >  2:2535816/1‑150 (MQ=255)
                                                                                                                                    tCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCatat              >  1:2266865/1‑150 (MQ=255)
                                                                                                                                        cgcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgc          >  2:3376951/1‑150 (MQ=255)
                                                                                                                                         gcTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgcc         >  1:2711526/1‑150 (MQ=255)
                                                                                                                                          cTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgccg        >  1:346989/1‑150 (MQ=255)
                                                                                                                                          cTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgccg        <  1:3087370/150‑1 (MQ=255)
                                                                                                                                          cTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgccg        >  2:1878114/1‑150 (MQ=255)
                                                                                                                                          cTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgccg        >  2:143818/1‑150 (MQ=255)
                                                                                                                                                tGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGt  <  1:634709/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGT  >  CP009273/479097‑479390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGT  >  CP009273/479095‑479393
                                                                                                                                                     |                                                                                                                                                     
CTGATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCCGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGA                                                                                                                                                       >  SRR19973072.4271725/1‑150 (MQ=60)
CTGATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGA                                                                                                                                                       >  SRR19973072.4271672/1‑150 (MQ=60)
CTGATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGA                                                                                                                                                       >  SRR19973072.2685546/1‑150 (MQ=60)
CTGATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGA                                                                                                                                                       >  SRR19973072.4271734/1‑150 (MQ=60)
 TGATTCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAG                                                                                                                                                      >  SRR19973072.449090/1‑150 (MQ=60)
  GATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGG                                                                                                                                                     <  SRR19973072.2430077/150‑1 (MQ=60)
    TTCACTTTCAGCAGTATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTA                                                                                                                                                   <  SRR19973072.483641/150‑1 (MQ=60)
     TCACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAA                                                                                                                                                  >  SRR19973072.436986/1‑150 (MQ=60)
      CACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAT                                                                                                                                                 <  SRR19973072.1921023/150‑1 (MQ=60)
      CACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAAGAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAT                                                                                                                                                 <  SRR19973072.4497695/150‑1 (MQ=60)
       ACTTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATG                                                                                                                                                >  SRR19973072.3857075/1‑150 (MQ=60)
        CTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGA                                                                                                                                               <  SRR19973072.1778046/150‑1 (MQ=60)
         TTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAC                                                                                                                                              >  SRR19973072.41650/1‑150 (MQ=60)
         TTTCAGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAC                                                                                                                                              <  SRR19973072.3552565/150‑1 (MQ=60)
           TCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAACCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACAA                                                                                                                                            >  SRR19973072.1436493/1‑150 (MQ=60)
             AGCAGGATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCA                                                                                                                                          >  SRR19973072.1214558/1‑150 (MQ=60)
               CAGGATTTTGCCTAACTCTTCAGGAGAGGTCAGACGCGTCTGAGCACTAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAC                                                                                                                                        >  SRR19973072.1533405/1‑150 (MQ=60)
                AGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACC                                                                                                                                       <  SRR19973072.2568214/150‑1 (MQ=60)
                AGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACC                                                                                                                                       <  SRR19973072.2568208/150‑1 (MQ=60)
                AGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACC                                                                                                                                       <  SRR19973072.4470240/150‑1 (MQ=60)
                   ATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGC                                                                                                                                    >  SRR19973072.4479944/1‑150 (MQ=60)
                   ATTTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGC                                                                                                                                    >  SRR19973072.543407/1‑150 (MQ=60)
                   ATTTTGCATAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAATCTGTTGGCCATTCAACGGCGGCGTAACAACGAGCTGACCCGCCGCAACCTGGGCGTTCTTCGCTTTTATGGAGGTAATGACATCAAACGGC                                                                                                                                    >  SRR19973072.543478/1‑150 (MQ=60)
                     TTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGT                                                                                                                                  <  SRR19973072.2743383/150‑1 (MQ=60)
                     TTTGCCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGT                                                                                                                                  <  SRR19973072.2743531/150‑1 (MQ=60)
                       TGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTA                                                                                                                                <  SRR19973072.2100938/150‑1 (MQ=60)
                         CCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC                                                                                                                              >  SRR19973072.44994/1‑150 (MQ=60)
                         CCTAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC                                                                                                                              >  SRR19973072.599378/1‑150 (MQ=60)
                           TAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTG                                                                                                                            >  SRR19973072.1094636/1‑150 (MQ=60)
                            AACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGG                                                                                                                           <  SRR19973072.4607714/150‑1 (MQ=60)
                                CTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATT                                                                                                                       <  SRR19973072.4217169/150‑1 (MQ=60)
                                       AGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAG                                                                                                                >  SRR19973072.2290628/1‑150 (MQ=60)
                                        GAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAATCTGTTTGCCTTTCACCGGCGGCGTACCACCGAGCAGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGATGTAATGACAACAACCGGCGTTAGCTGGAATTTGTTCAGC                                                                                                               >  SRR19973072.2287312/1‑150 (MQ=60)
                                        GAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGC                                                                                                               >  SRR19973072.1726001/1‑150 (MQ=60)
                                        GAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTAGTTCAGC                                                                                                               >  SRR19973072.1726003/1‑150 (MQ=60)
                                         AGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCT                                                                                                              >  SRR19973072.2749644/1‑150 (MQ=60)
                                           GTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCA                                                                                                            <  SRR19973072.1781819/150‑1 (MQ=60)
                                            TCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCTATCTGACCCTCCGCAACCTGGGCTTTCTTCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAT                                                                                                           <  SRR19973072.2398287/150‑1 (MQ=60)
                                            TCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAT                                                                                                           <  SRR19973072.2398316/150‑1 (MQ=60)
                                             CAGACGCGTCTGAGCAATGATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATT                                                                                                          <  SRR19973072.3803082/150‑1 (MQ=60)
                                              AGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTC                                                                                                         <  SRR19973072.168754/150‑1 (MQ=60)
                                              AGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTC                                                                                                         <  SRR19973072.3853975/150‑1 (MQ=60)
                                                ACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGG                                                                                                       <  SRR19973072.4134016/150‑1 (MQ=60)
                                                    GTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTC                                                                                                   >  SRR19973072.3981643/1‑150 (MQ=60)
                                                      CTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCAT                                                                                                 >  SRR19973072.3923929/1‑150 (MQ=60)
                                                      CTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCAT                                                                                                 >  SRR19973072.3188463/1‑150 (MQ=60)
                                                      CTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCAT                                                                                                 <  SRR19973072.364385/150‑1 (MQ=60)
                                                      CTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTAAGCTCATTCGGGTTCAT                                                                                                 >  SRR19973072.1954392/1‑150 (MQ=60)
                                                       TGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATC                                                                                                >  SRR19973072.1548463/1‑150 (MQ=60)
                                                       TGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATC                                                                                                >  SRR19973072.2320402/1‑150 (MQ=60)
                                                       TGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATC                                                                                                <  SRR19973072.3047578/150‑1 (MQ=60)
                                                       TGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATA                                                                                                >  SRR19973072.2320514/1‑150 (MQ=60)
                                                          GCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAG                                                                                             >  SRR19973072.2709698/1‑150 (MQ=60)
                                                          GCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATACAG                                                                                             >  SRR19973072.2709651/1‑150 (MQ=60)
                                                           CAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGA                                                                                            <  SRR19973072.3320478/150‑1 (MQ=60)
                                                            AATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAT                                                                                           >  SRR19973072.1082399/1‑150 (MQ=60)
                                                              TAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAC                                                                                         <  SRR19973072.3617314/150‑1 (MQ=60)
                                                               AATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACG                                                                                        >  SRR19973072.506660/1‑150 (MQ=60)
                                                                    AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                   <  SRR19973072.2938347/150‑1 (MQ=60)
                                                                    AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                   <  SRR19973072.2938450/150‑1 (MQ=60)
                                                                          TAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACT                                                                             <  SRR19973072.225872/150‑1 (MQ=60)
                                                                             GCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTG                                                                          <  SRR19973072.1501586/150‑1 (MQ=60)
                                                                              CTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGA                                                                         <  SRR19973072.2788695/150‑1 (MQ=60)
                                                                              CTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGA                                                                         <  SRR19973072.1306551/150‑1 (MQ=60)
                                                                               TGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAA                                                                        >  SRR19973072.4192086/1‑150 (MQ=60)
                                                                                GTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAC                                                                       <  SRR19973072.3391226/150‑1 (MQ=60)
                                                                                  TGGCCTTTCATCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCG                                                                     <  SRR19973072.781830/150‑1 (MQ=60)
                                                                                  TGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCG                                                                     <  SRR19973072.4416372/150‑1 (MQ=60)
                                                                                      CTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACA                                                                 >  SRR19973072.2067604/1‑150 (MQ=60)
                                                                                      CTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACA                                                                 <  SRR19973072.4602938/150‑1 (MQ=60)
                                                                                          CACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTG                                                             <  SRR19973072.1535718/150‑1 (MQ=60)
                                                                                            CCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAA                                                           <  SRR19973072.638855/150‑1 (MQ=60)
                                                                                              GGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACA                                                         <  SRR19973072.313672/150‑1 (MQ=60)
                                                                                              GGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACA                                                         <  SRR19973072.602376/150‑1 (MQ=60)
                                                                                                 GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                      >  SRR19973072.4217703/1‑150 (MQ=60)
                                                                                                 GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                      >  SRR19973072.4217743/1‑150 (MQ=60)
                                                                                                 GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                      >  SRR19973072.4217748/1‑150 (MQ=60)
                                                                                                 GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                      >  SRR19973072.2374965/1‑150 (MQ=60)
                                                                                                 GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                      >  SRR19973072.2433537/1‑150 (MQ=60)
                                                                                                    GTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCC                                                   >  SRR19973072.2494049/1‑150 (MQ=60)
                                                                                                    GTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCC                                                   >  SRR19973072.1141943/1‑150 (MQ=60)
                                                                                                        CACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGC                                               >  SRR19973072.932545/1‑150 (MQ=60)
                                                                                                          CCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCC                                             <  SRR19973072.805138/150‑1 (MQ=60)
                                                                                                          CCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCC                                             <  SRR19973072.1283184/150‑1 (MQ=60)
                                                                                                          CCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCC                                             <  SRR19973072.804872/150‑1 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACACCTGAACATCACCCACGCCCG                                            <  SRR19973072.1005180/150‑1 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCG                                            <  SRR19973072.3212665/150‑1 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCG                                            <  SRR19973072.3212664/150‑1 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCG                                            >  SRR19973072.3170740/1‑150 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCG                                            >  SRR19973072.3170568/1‑150 (MQ=60)
                                                                                                           CGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCG                                            >  SRR19973072.3170562/1‑150 (MQ=60)
                                                                                                            GAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGA                                           <  SRR19973072.1823697/150‑1 (MQ=60)
                                                                                                            GAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGA                                           <  SRR19973072.4391882/150‑1 (MQ=60)
                                                                                                            GAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGA                                           <  SRR19973072.4394318/150‑1 (MQ=60)
                                                                                                             AGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGAC                                          <  SRR19973072.714671/150‑1 (MQ=60)
                                                                                                               CTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTAATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGT                                        <  SRR19973072.2589880/150‑1 (MQ=60)
                                                                                                                TGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTA                                       >  SRR19973072.2245683/1‑150 (MQ=60)
                                                                                                                 GACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAC                                      >  SRR19973072.296906/1‑150 (MQ=60)
                                                                                                                    CCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGC                                   <  SRR19973072.1915735/150‑1 (MQ=60)
                                                                                                                     CGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGACTTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCT                                  <  SRR19973072.510507/150‑1 (MQ=60)
                                                                                                                     CGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCT                                  <  SRR19973072.2398564/150‑1 (MQ=60)
                                                                                                                     CGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCT                                  <  SRR19973072.2832920/150‑1 (MQ=60)
                                                                                                                     CGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCT                                  <  SRR19973072.4420694/150‑1 (MQ=60)
                                                                                                                     CGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCT                                  <  SRR19973072.1433768/150‑1 (MQ=60)
                                                                                                                       CCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGA                                <  SRR19973072.3465293/150‑1 (MQ=60)
                                                                                                                         GCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATG                              <  SRR19973072.122727/150‑1 (MQ=60)
                                                                                                                         GCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATG                              >  SRR19973072.3315633/1‑150 (MQ=60)
                                                                                                                               TGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCT                        >  SRR19973072.3994718/1‑150 (MQ=60)
                                                                                                                                GGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTT                       <  SRR19973072.2929433/150‑1 (MQ=60)
                                                                                                                                GGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTT                       <  SRR19973072.3985057/150‑1 (MQ=60)
                                                                                                                                 GGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTT                      >  SRR19973072.1926465/1‑150 (MQ=60)
                                                                                                                                    GTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCAT                   <  SRR19973072.3005103/150‑1 (MQ=60)
                                                                                                                                    GTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCAT                   <  SRR19973072.1729356/150‑1 (MQ=60)
                                                                                                                                      TCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAT                 >  SRR19973072.1226937/1‑150 (MQ=60)
                                                                                                                                      TCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAT                 >  SRR19973072.2271668/1‑150 (MQ=60)
                                                                                                                                      TCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAT                 >  SRR19973072.2271738/1‑150 (MQ=60)
                                                                                                                                          CGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGC             >  SRR19973072.1837793/1‑150 (MQ=60)
                                                                                                                                           GCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCC            >  SRR19973072.2717306/1‑150 (MQ=60)
                                                                                                                                           GCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCC            >  SRR19973072.394767/1‑150 (MQ=60)
                                                                                                                                            CTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCG           <  SRR19973072.1435316/150‑1 (MQ=60)
                                                                                                                                            CTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCG           >  SRR19973072.347766/1‑150 (MQ=60)
                                                                                                                                            CTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCG           <  SRR19973072.3093875/150‑1 (MQ=60)
                                                                                                                                               TGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCA        <  SRR19973072.3719930/150‑1 (MQ=60)
                                                                                                                                                  TGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGT     >  SRR19973072.1472043/1‑150 (MQ=60)
                                                                                                                                                  TGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGT     >  SRR19973072.1652230/1‑150 (MQ=60)
                                                                                                                                                  TGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGT     <  SRR19973072.636157/150‑1 (MQ=60)
                                                                                                                                                    GAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG   <  SRR19973072.2014323/150‑1 (MQ=60)
                                                                                                                                                     AGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGT  <  SRR19973072.1400046/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGT  >  CP009273/479095‑479393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑