Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 479,244 C→A 100% A206S (GCC→TCC)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273479,2440CA99.5% 676.6 / ‑5.1 218A206S (GCC→TCC) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (108/108);  minor base T (2/0);  total (110/108)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCA  >  CP009273/479099‑479387
                                                                                                                                                 |                                                                                                                                                
 tCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGt                                                                                                                                               <  1:173785/148‑1 (MQ=255)
  cACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTa                                                                                                                                              <  1:3540849/148‑1 (MQ=255)
     tttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATg                                                                                                                                           <  1:19913/148‑1 (MQ=255)
        cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACa                                                                                                                                        <  1:897723/148‑1 (MQ=255)
           cagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCa                                                                                                                                     <  2:2391258/148‑1 (MQ=255)
           cagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCa                                                                                                                                     >  2:1482632/1‑148 (MQ=255)
            agGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCaa                                                                                                                                    <  2:3784205/148‑1 (MQ=255)
              gATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAcc                                                                                                                                  <  2:3668041/148‑1 (MQ=255)
              gATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAcc                                                                                                                                  >  1:3140352/1‑148 (MQ=255)
              gATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAAcc                                                                                                                                  <  2:2135354/148‑1 (MQ=255)
                   tGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGt                                                                                                                             <  2:3322215/148‑1 (MQ=255)
                    gCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGtt                                                                                                                            <  2:2711931/148‑1 (MQ=255)
                      cGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAg                                                                                                                          <  1:3087537/148‑1 (MQ=255)
                        aaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑t                                                                                                                       >  1:157951/1‑148 (MQ=255)
                        aaCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑t                                                                                                                       >  2:2538781/1‑148 (MQ=255)
                            ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGaa                                                                                                                   <  1:1391336/148‑1 (MQ=255)
                              tCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCCCCGCAACCGGGGCGGTCTGCGCGTTGTTGGTGGGAAATACAAAAACCCGCGGTATC‑AGGAAtt                                                                                                                 >  1:1616583/1‑148 (MQ=255)
                                aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTg                                                                                                               <  2:3100865/148‑1 (MQ=255)
                                aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTg                                                                                                               <  2:1196078/148‑1 (MQ=255)
                                   agagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGGTAGC‑TGGACTTTGTTc                                                                                                            >  2:1794476/1‑148 (MQ=255)
                                    gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCa                                                                                                           >  1:1885552/1‑148 (MQ=255)
                                    gagGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCa                                                                                                           >  1:954885/1‑148 (MQ=255)
                                     agGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAg                                                                                                          >  2:2869406/1‑148 (MQ=255)
                                     agGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAg                                                                                                          <  1:1908284/148‑1 (MQ=255)
                                       gTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCt                                                                                                        >  2:738967/1‑148 (MQ=255)
                                       gTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCt                                                                                                        >  2:110609/1‑148 (MQ=255)
                                          agtcgcgTCTGAGCTATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCAt                                                                                                     <  1:3562568/145‑1 (MQ=255)
                                          aGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCAt                                                                                                     >  2:1493936/1‑148 (MQ=255)
                                           gACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCAtt                                                                                                    >  2:2490599/1‑148 (MQ=255)
                                                gTCTGAGCAATACTAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGt                                                                                               <  1:2095315/148‑1 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTTATGGAGGTAATGACATCAACCCGCGTTAGC‑TTGAATTTGTTCAGCTCATTAGGGTTc                                                                                             >  1:3258937/1‑148 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTc                                                                                             <  2:2915952/148‑1 (MQ=255)
                                                  cTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTc                                                                                             <  2:865140/148‑1 (MQ=255)
                                                      gCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATcc                                                                                         <  2:3779078/148‑1 (MQ=255)
                                                       cAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCa                                                                                        >  1:1894079/1‑148 (MQ=255)
                                                        aataatAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAg                                                                                       <  1:1810404/148‑1 (MQ=255)
                                                        aataatAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAg                                                                                       <  1:165407/148‑1 (MQ=255)
                                                        aataatAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAg                                                                                       >  1:3638851/1‑148 (MQ=255)
                                                         ataataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGa                                                                                      <  1:806684/148‑1 (MQ=255)
                                                         ataataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTATGCGCGTTGATGGAGGTAATGACATCAAACCGCGTTAGC‑TGGAATTTTTTAAGCGCATTAGGGTTCATACAGa                                                                                      >  1:822159/1‑148 (MQ=255)
                                                          taataGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAt                                                                                     >  2:1382827/1‑148 (MQ=255)
                                                             taGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGTTGACCCCCCGCAACCAGGGCGTTCTGCGCGTTTTTGGAGGTAAAGACAAAAAACCGCGTTATCATGGAATTT‑TTAATCTCAATAGGGTTAATACAAATAcc                                                                                  >  1:2581446/1‑147 (MQ=255)
                                                             taGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATTACATAAACCCGCGTTAGC‑TGGTATTTGTTAAGCTCATTAGGGTTCATACAGATACg                                                                                  >  1:61661/1‑148 (MQ=255)
                                                             taGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATTACAACAAACGGCGTTAGC‑TGGAAATTTTTCACCTCAATAGGGTTCATACAGATaag                                                                                  >  1:2940984/1‑146 (MQ=255)
                                                              agagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGTAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc                                                                                 <  2:2381042/148‑1 (MQ=255)
                                                              agagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc                                                                                 <  2:2209711/148‑1 (MQ=255)
                                                              agagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc                                                                                 >  2:3764689/1‑148 (MQ=255)
                                                               gagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑AGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCa                                                                                >  2:459934/1‑148 (MQ=255)
                                                                  gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                             >  1:3161989/1‑148 (MQ=255)
                                                                  gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                             >  1:2385592/1‑148 (MQ=255)
                                                                  gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg                                                                             >  1:1256799/1‑148 (MQ=255)
                                                                     ttAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTTTTCAGCTCATTCGGGTTCATCAAGATACTCAACGCGt                                                                          >  1:2310223/1‑148 (MQ=255)
                                                                     ttAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGt                                                                          >  1:3738483/1‑148 (MQ=255)
                                                                         gCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtg                                                                      >  1:3597109/1‑148 (MQ=255)
                                                                         gCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtg                                                                      <  1:2914572/148‑1 (MQ=255)
                                                                          cTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgt                                                                     >  2:1074532/1‑148 (MQ=255)
                                                                          cTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgt                                                                     >  1:1548240/1‑148 (MQ=255)
                                                                            gTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGa                                                                   >  1:1082796/1‑148 (MQ=255)
                                                                             ttGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGaa                                                                  <  1:1118797/148‑1 (MQ=255)
                                                                               ggCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAcc                                                                <  1:3820246/148‑1 (MQ=255)
                                                                                 ccTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGa                                                              >  2:2972984/1‑148 (MQ=255)
                                                                                 ccTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGa                                                              <  1:3180227/148‑1 (MQ=255)
                                                                                     tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaa                                                          <  1:1481034/148‑1 (MQ=255)
                                                                                     tCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGaacaa                                                          <  2:3746722/148‑1 (MQ=255)
                                                                                        ccGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTg                                                       >  2:508039/1‑148 (MQ=255)
                                                                                            cggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACa                                                   <  2:1883309/148‑1 (MQ=255)
                                                                                             ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACAt                                                  <  1:3766665/148‑1 (MQ=255)
                                                                                               cgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCa                                                <  1:2348477/148‑1 (MQ=255)
                                                                                                      ccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGc                                         >  2:794195/1‑148 (MQ=255)
                                                                                                              aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACCACTGAACATCACCCACCCCCGACGTa                                 >  1:45630/1‑148 (MQ=255)
                                                                                                              aCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTa                                 <  1:1793035/148‑1 (MQ=255)
                                                                                                               cccGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAc                                >  1:744210/1‑148 (MQ=255)
                                                                                                                    cgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTg                           <  2:1256799/148‑1 (MQ=255)
                                                                                                                    cgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTg                           <  2:3161989/148‑1 (MQ=255)
                                                                                                                    cgcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTg                           <  2:2385592/148‑1 (MQ=255)
                                                                                                                     gcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGa                          <  2:2489726/148‑1 (MQ=255)
                                                                                                                     gcAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGa                          <  2:623072/148‑1 (MQ=255)
                                                                                                                       aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                        <  2:335752/148‑1 (MQ=255)
                                                                                                                       aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                        <  2:1616583/148‑1 (MQ=255)
                                                                                                                       aaCCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATg                        <  1:627818/148‑1 (MQ=255)
                                                                                                                         ccTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGc                      >  1:242021/1‑148 (MQ=255)
                                                                                                                           tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAt                    <  1:2490599/148‑1 (MQ=255)
                                                                                                                           tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAt                    >  1:3457597/1‑148 (MQ=255)
                                                                                                                           tGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAt                    <  2:3203439/148‑1 (MQ=255)
                                                                                                                            gggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATc                   >  1:2727196/1‑148 (MQ=255)
                                                                                                                            gggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATc                   <  2:1832072/148‑1 (MQ=255)
                                                                                                                             ggCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCt                  >  1:2609042/1‑148 (MQ=255)
                                                                                                                              gCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCtt                 >  2:493334/1‑148 (MQ=255)
                                                                                                                               cGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCttt                <  2:3114061/148‑1 (MQ=255)
                                                                                                                                gTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTc               <  1:1484824/148‑1 (MQ=255)
                                                                                                                                gTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTc               <  2:1906580/148‑1 (MQ=255)
                                                                                                                                  tCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGCATTTGGTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat             <  2:2773461/148‑1 (MQ=255)
                                                                                                                                  tCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat             <  2:3020836/148‑1 (MQ=255)
                                                                                                                                   cTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCata            <  2:3605408/148‑1 (MQ=255)
                                                                                                                                   cTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCata            <  1:2818912/148‑1 (MQ=255)
                                                                                                                                     gcgcTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAtt          >  2:947957/1‑148 (MQ=255)
                                                                                                                                      cgcTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTc         >  2:429623/1‑148 (MQ=255)
                                                                                                                                        cTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgc       >  1:3317309/1‑148 (MQ=255)
                                                                                                                                        cTTTGATGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcgc       >  2:2296095/1‑148 (MQ=255)
                                                                                                                                             aTGGAGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCa  <  1:2538781/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGC‑TGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCA  >  CP009273/479099‑479387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  >  CP009273/479096‑479392
                                                                                                                                                    |                                                                                                                                                    
TGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAG                                                                                                                                                     <  SRR19973069.979122/150‑1 (MQ=60)
TGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAG                                                                                                                                                     <  SRR19973069.2944152/150‑1 (MQ=60)
 GATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGG                                                                                                                                                    <  SRR19973069.2134298/150‑1 (MQ=60)
   TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTA                                                                                                                                                  <  SRR19973069.1457344/150‑1 (MQ=60)
   TTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTA                                                                                                                                                  <  SRR19973069.11613/150‑1 (MQ=60)
    TCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAA                                                                                                                                                 <  SRR19973069.175255/150‑1 (MQ=60)
     CACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAT                                                                                                                                                >  SRR19973069.3367785/1‑150 (MQ=60)
     CACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAT                                                                                                                                                <  SRR19973069.3563779/150‑1 (MQ=60)
     CACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAAT                                                                                                                                                <  SRR19973069.3563822/150‑1 (MQ=60)
        TTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAC                                                                                                                                             <  SRR19973069.20200/150‑1 (MQ=60)
        TTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGAC                                                                                                                                             <  SRR19973069.19732/150‑1 (MQ=60)
           CAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAATCTGTTGGCCGTTCACCCGCGGCGTACCACCGAGCTGACCCCCCCCAACCTGGGCGTTCTGCGCGTTGGTGGTGGTAATGACATA                                                                                                                                          >  SRR19973069.2263126/1‑150 (MQ=60)
           CAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATC                                                                                                                                          <  SRR19973069.903903/150‑1 (MQ=60)
           CAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATC                                                                                                                                          <  SRR19973069.904105/150‑1 (MQ=60)
            AGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCA                                                                                                                                         >  SRR19973069.1521936/1‑150 (MQ=60)
               AGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACC                                                                                                                                      >  SRR19973069.3160913/1‑150 (MQ=60)
                       GCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAG                                                                                                                              >  SRR19973069.972512/1‑150 (MQ=60)
                         CGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCT                                                                                                                            <  SRR19973069.3107895/150‑1 (MQ=60)
                         CGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCT                                                                                                                            >  SRR19973069.159337/1‑150 (MQ=60)
                         CGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTAACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCT                                                                                                                            >  SRR19973069.158249/1‑150 (MQ=60)
                            ACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGA                                                                                                                         <  SRR19973069.2386962/150‑1 (MQ=60)
                            ACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACAACAACCCGCGTTAGCTGGA                                                                                                                         >  SRR19973069.524430/1‑150 (MQ=60)
                               CTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATT                                                                                                                      <  SRR19973069.1401036/150‑1 (MQ=60)
                               CTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATT                                                                                                                      <  SRR19973069.1400930/150‑1 (MQ=60)
                                     TAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCA                                                                                                                >  SRR19973069.1898643/1‑150 (MQ=60)
                                     TAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCAGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCA                                                                                                                >  SRR19973069.961287/1‑150 (MQ=60)
                                        AGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCT                                                                                                             <  SRR19973069.1921461/150‑1 (MQ=60)
                                           TCAGACGCGTCTGAGCAATAATAGAGGCGTTAATCTGTTGGCCTTTCACCCGCGGCGTACCACCGAGCTGACCCCCCCCAACCAGGGCGTTGTGGGCGTTGTTGGTGGTAATTACATCAAACGGCGTTAtgtggaattttttaagatgat                                                                                                          >  SRR19973069.10563/1‑129 (MQ=60)
                                            CAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTTTTCAGCTCATT                                                                                                         >  SRR19973069.475576/1‑150 (MQ=60)
                                             AGTCGCGTCTGAGCTATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTC                                                                                                        <  SRR19973069.3585761/150‑1 (MQ=60)
                                             AGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTC                                                                                                        <  SRR19973069.3585746/150‑1 (MQ=60)
                                                   GTCTGAGCAATCATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAATTGTTCAGCTCATTCGGGTTC                                                                                                  <  SRR19973069.2942530/150‑1 (MQ=60)
                                                   GTCTGAGCAATACTAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTC                                                                                                  <  SRR19973069.2109798/150‑1 (MQ=60)
                                                   GTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTTATGGAGGTAATGACATCAACCCGCGTTAGCTTGAATTTGTTCAGCTCATTAGGGTTC                                                                                                  >  SRR19973069.3280283/1‑150 (MQ=60)
                                                   GTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTC                                                                                                  <  SRR19973069.166169/150‑1 (MQ=60)
                                                     CTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCCGCGTTAGCTGGAATTTTTTAAGCTCAATCGGGTTCAT                                                                                                >  SRR19973069.3240652/1‑150 (MQ=60)
                                                        AGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCA                                                                                             >  SRR19973069.1906861/1‑150 (MQ=60)
                                                        AGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCAACCA                                                                                             >  SRR19973069.1907200/1‑150 (MQ=60)
                                                        AGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCA                                                                                             >  SRR19973069.1907199/1‑150 (MQ=60)
                                                         GCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAG                                                                                            >  SRR19973069.3662676/1‑150 (MQ=60)
                                                         GCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAG                                                                                            <  SRR19973069.1785269/150‑1 (MQ=60)
                                                          CAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTATGCGCGTTGATGGAGGTAATGACATCAAACCGCGTTAGCTGGAATTTTTTAAGCGCATTAGGGTTCATACAGA                                                                                           >  SRR19973069.827963/1‑150 (MQ=60)
                                                          CAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTAACACCGAGCTGACCCGCCGCCACCTGGGCGTTCTGCGCTTTGATGGAGGTAAAGACATCAAACCGCGTTATCTGGTATTTGTTAAGCTCATTAGGGTTCATacata                                                                                           >  SRR19973069.2600752/1‑145 (MQ=60)
                                                           AATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAT                                                                                          <  SRR19973069.1823132/150‑1 (MQ=60)
                                                           AATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGAT                                                                                          <  SRR19973069.166834/150‑1 (MQ=60)
                                                           AATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGA‑CCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACCTCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATACAGATA                                                                                         >  SRR19973069.2031202/1‑150 (MQ=60)
                                                            ATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATA                                                                                         <  SRR19973069.2538163/150‑1 (MQ=60)
                                                            ATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATA                                                                                         <  SRR19973069.812400/150‑1 (MQ=60)
                                                            ATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATA                                                                                         <  SRR19973069.812475/150‑1 (MQ=60)
                                                            ATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAAATACAACAAACCGCGTTAGCTGGAATTTGTTAAGCTCATTAGGGTTCATACAGATA                                                                                         >  SRR19973069.2731241/1‑150 (MQ=60)
                                                              AATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATTACATAAACCCGCGTTAGCTGGTATTTGTTAAGCTCATTAGGGTTCATACAGATACG                                                                                       >  SRR19973069.62390/1‑150 (MQ=60)
                                                              AATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATTACAACAAACGGCGTTAGCTGGAAATTTTTCACCTCAATAGGGTTCATACAGATAAG                                                                                       >  SRR19973069.2960487/1‑150 (MQ=60)
                                                                   AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                  >  SRR19973069.2401763/1‑150 (MQ=60)
                                                                   AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                  >  SRR19973069.1265286/1‑150 (MQ=60)
                                                                   AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                  >  SRR19973069.3182618/1‑150 (MQ=60)
                                                                   AGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCG                                                                                  >  SRR19973069.3182783/1‑150 (MQ=60)
                                                                      CGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTTTTCAGCTCATTCGGGTTCATCAAGATACTCAACGCGT                                                                               >  SRR19973069.2325940/1‑150 (MQ=60)
                                                                      CGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGT                                                                               >  SRR19973069.3762850/1‑150 (MQ=60)
                                                                      CGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGT                                                                               >  SRR19973069.3640737/1‑150 (MQ=60)
                                                                        TTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTAC                                                                             <  SRR19973069.3333276/150‑1 (MQ=60)
                                                                         TAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACT                                                                            <  SRR19973069.1607374/150‑1 (MQ=60)
                                                                          AAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTG                                                                           >  SRR19973069.3620593/1‑150 (MQ=60)
                                                                          AAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTG                                                                           >  SRR19973069.3714345/1‑150 (MQ=60)
                                                                           AGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGT                                                                          >  SRR19973069.1558503/1‑150 (MQ=60)
                                                                           AGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGT                                                                          >  SRR19973069.1558829/1‑150 (MQ=60)
                                                                            GCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTG                                                                         <  SRR19973069.2933860/150‑1 (MQ=60)
                                                                             CTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGA                                                                        >  SRR19973069.1090424/1‑150 (MQ=60)
                                                                             CTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGA                                                                        >  SRR19973069.3615463/1‑150 (MQ=60)
                                                                             CTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGA                                                                        >  SRR19973069.1090356/1‑150 (MQ=60)
                                                                               GTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAC                                                                      <  SRR19973069.1820533/150‑1 (MQ=60)
                                                                                TTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACC                                                                     <  SRR19973069.1126599/150‑1 (MQ=60)
                                                                                TTGGCCTGTCACCGGCGGCGTACCTCCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACC                                                                     <  SRR19973069.2869158/150‑1 (MQ=60)
                                                                                  GGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGA                                                                   <  SRR19973069.3844961/150‑1 (MQ=60)
                                                                                   GCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAA                                                                  >  SRR19973069.89144/1‑150 (MQ=60)
                                                                                   GCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAA                                                                  >  SRR19973069.3116270/1‑150 (MQ=60)
                                                                                    CCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAAC                                                                 <  SRR19973069.3200911/150‑1 (MQ=60)
                                                                                    CCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATATGTTCAGCTCATTCGGGTTCATCCAGATAAGAATCGCGTACTGTGAACCGAAC                                                                 >  SRR19973069.3607792/1‑150 (MQ=60)
                                                                                      TTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAA                                                               >  SRR19973069.2575057/1‑150 (MQ=60)
                                                                                       TTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAAC                                                              <  SRR19973069.3257933/150‑1 (MQ=60)
                                                                                        TCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACT                                                             <  SRR19973069.1491336/150‑1 (MQ=60)
                                                                                         CACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTG                                                            <  SRR19973069.727681/150‑1 (MQ=60)
                                                                                             GGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACA                                                        >  SRR19973069.1690979/1‑150 (MQ=60)
                                                                                               CGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATC                                                      >  SRR19973069.1867077/1‑150 (MQ=60)
                                                                                                GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                     <  SRR19973069.3791145/150‑1 (MQ=60)
                                                                                                GGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCA                                                     <  SRR19973069.3791364/150‑1 (MQ=60)
                                                                                                  CGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACC                                                   <  SRR19973069.2364454/150‑1 (MQ=60)
                                                                                                  CGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACC                                                   <  SRR19973069.2363546/150‑1 (MQ=60)
                                                                                                  CGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACC                                                   <  SRR19973069.1492943/150‑1 (MQ=60)
                                                                                                    TACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCA                                                 <  SRR19973069.3767702/150‑1 (MQ=60)
                                                                                                        ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATAAGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCC                                             >  SRR19973069.2932657/1‑150 (MQ=60)
                                                                                                               TGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACCACTGAACATCACCCACCCCCGACGTA                                      >  SRR19973069.46213/1‑150 (MQ=60)
                                                                                                               TGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTAGGGTTCATACAGATAAGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTA                                      >  SRR19973069.46141/1‑150 (MQ=60)
                                                                                                                GACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTAC                                     >  SRR19973069.749478/1‑150 (MQ=60)
                                                                                                                 ACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACG                                    <  SRR19973069.1805665/150‑1 (MQ=60)
                                                                                                                       CGCAACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGAT                              <  SRR19973069.208014/150‑1 (MQ=60)
                                                                                                                          AACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGC                           <  SRR19973069.631933/150‑1 (MQ=60)
                                                                                                                          AACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGC                           <  SRR19973069.631978/150‑1 (MQ=60)
                                                                                                                          AACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGC                           >  SRR19973069.243828/1‑150 (MQ=60)
                                                                                                                          AACCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAAATTGTTCAGCACATTCGGGTTCATACAGATAACCATCGCGTACTGTGAACCCAACAACAGAACAAAACCCACCCCCCACCTACGGCTGATGGC                           >  SRR19973069.1104291/1‑150 (MQ=60)
                                                                                                                            CCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAT                         >  SRR19973069.3479989/1‑150 (MQ=60)
                                                                                                                            CCTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCAT                         >  SRR19973069.3161620/1‑150 (MQ=60)
                                                                                                                             CTGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATC                        >  SRR19973069.2745461/1‑150 (MQ=60)
                                                                                                                              TGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCT                       >  SRR19973069.2626483/1‑150 (MQ=60)
                                                                                                                              TGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCT                       <  SRR19973069.2506954/150‑1 (MQ=60)
                                                                                                                              TGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCT                       <  SRR19973069.2507261/150‑1 (MQ=60)
                                                                                                                              TGGGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCT                       <  SRR19973069.752227/150‑1 (MQ=60)
                                                                                                                                GGCGTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTT                     <  SRR19973069.24207/150‑1 (MQ=60)
                                                                                                                                  CGTTCTGCGCGTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCA                   >  SRR19973069.1820721/1‑150 (MQ=60)
                                                                                                                                   GTTCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCAT                  <  SRR19973069.1495150/150‑1 (MQ=60)
                                                                                                                                     TCTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATAT                <  SRR19973069.898093/150‑1 (MQ=60)
                                                                                                                                      CTGCGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATT               <  SRR19973069.2837569/150‑1 (MQ=60)
                                                                                                                                         CGCTTTGATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGC            >  SRR19973069.3339055/1‑150 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     <  SRR19973069.2555571/150‑1 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     >  SRR19973069.2551129/1‑150 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     <  SRR19973069.2555592/150‑1 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     >  SRR19973069.620373/1‑150 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     >  SRR19973069.620302/1‑150 (MQ=60)
                                                                                                                                                ATGGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACG     >  SRR19973069.620297/1‑150 (MQ=60)
                                                                                                                                                  GGAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTA   <  SRR19973069.511665/150‑1 (MQ=60)
                                                                                                                                                   GAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  >  SRR19973069.106922/1‑150 (MQ=60)
                                                                                                                                                   GAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  <  SRR19973069.1943192/150‑1 (MQ=60)
                                                                                                                                                   GAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  <  SRR19973069.2489106/150‑1 (MQ=60)
                                                                                                                                                   GAGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  <  SRR19973069.595056/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAG  >  CP009273/479096‑479392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑