Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 498,579 G→T 19.0% A39E (GCG→GAG)  ybaL ← inner membrane putative NAD(P)‑binding transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273498,5790GT19.0% 831.6 / 123.9 278A39E (GCG→GAG) ybaLinner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base G (109/116);  new base T (31/21);  total (141/137)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 281 total aligned reads displayed.

TGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTT  >  CP009273/498430‑498728
                                                                                                                                                     |                                                                                                                                                     
      tGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACa                                                                                                                                                 >  2:769852/1‑150 (MQ=255)
        gCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa                                                                                                                                               <  1:505618/150‑1 (MQ=255)
        gCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGa                                                                                                                                               <  1:3220847/150‑1 (MQ=255)
         ccTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGat                                                                                                                                              <  2:4157013/150‑1 (MQ=255)
         ccTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGat                                                                                                                                              >  1:3386666/1‑150 (MQ=255)
          cTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGata                                                                                                                                             <  2:1740663/150‑1 (MQ=255)
           tttACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGatat                                                                                                                                            <  2:1906892/150‑1 (MQ=255)
            ttACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATc                                                                                                                                           <  2:585008/150‑1 (MQ=255)
             tACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATcc                                                                                                                                          >  1:2066306/1‑150 (MQ=255)
              aCCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATccc                                                                                                                                         >  2:2402002/1‑150 (MQ=255)
              aCCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATccc                                                                                                                                         >  1:2948751/1‑150 (MQ=255)
               ccGCCATCAAATCCTTCAGCTAAAAGTGAAAACCGACGCCAACCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAGAGATATCcca                                                                                                                                        <  2:2089123/150‑1 (MQ=255)
                   cATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCag                                                                                                                                    <  2:2794346/150‑1 (MQ=255)
                    aTCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCaga                                                                                                                                   <  2:2512163/150‑1 (MQ=255)
                      cAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagg                                                                                                                                 >  1:4039120/1‑150 (MQ=255)
                       aaaTCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga                                                                                                                                >  2:526455/1‑150 (MQ=255)
                       aaaTCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAgagga                                                                                                                                <  2:3301043/150‑1 (MQ=255)
                           ccTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGaaa                                                                                                                            <  2:3869016/150‑1 (MQ=255)
                           ccTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa                                                                                                                            <  2:2848924/150‑1 (MQ=255)
                           ccTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGaaa                                                                                                                            >  1:671188/1‑150 (MQ=255)
                            cTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAAt                                                                                                                           >  1:262002/1‑150 (MQ=255)
                              tCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAc                                                                                                                         >  2:3094146/1‑150 (MQ=255)
                              tCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAc                                                                                                                         <  1:4487829/150‑1 (MQ=255)
                                aGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt                                                                                                                       >  2:866336/1‑150 (MQ=255)
                                 gCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTa                                                                                                                      <  2:3152272/150‑1 (MQ=255)
                                   gAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGAAGt                                                                                                                    >  1:663247/1‑150 (MQ=255)
                                    aaaaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGtt                                                                                                                   <  1:4232635/150‑1 (MQ=255)
                                     aaaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt                                                                                                                  >  1:973866/1‑150 (MQ=255)
                                     aaaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt                                                                                                                  <  1:1736583/150‑1 (MQ=255)
                                     aaaGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGttt                                                                                                                  >  2:3916680/1‑150 (MQ=255)
                                        gTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt                                                                                                               <  2:2840839/150‑1 (MQ=255)
                                        gTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAtt                                                                                                               <  1:1810061/150‑1 (MQ=255)
                                         tGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTg                                                                                                              >  2:4152832/1‑150 (MQ=255)
                                            aaaCCGACGCCAAACATCTACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTTACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc                                                                                                           <  2:4233540/150‑1 (MQ=255)
                                            aaaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGcc                                                                                                           >  2:1121509/1‑150 (MQ=255)
                                             aaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa                                                                                                          >  1:3973046/1‑150 (MQ=255)
                                             aaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACATATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa                                                                                                          <  2:1652406/150‑1 (MQ=255)
                                             aaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa                                                                                                          <  1:4358958/150‑1 (MQ=255)
                                             aaCCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCa                                                                                                          >  1:4105158/1‑150 (MQ=255)
                                               ccGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGc                                                                                                        <  2:1548543/150‑1 (MQ=255)
                                                cGACGCCAAACATCAACAGAATGACGCCCAATTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCa                                                                                                       >  1:4291044/1‑150 (MQ=255)
                                                 ttcGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCAt                                                                                                      <  2:4616923/148‑1 (MQ=255)
                                                 gACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCAt                                                                                                      >  1:4618225/1‑150 (MQ=255)
                                                      cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGa                                                                                                 >  1:3446347/1‑150 (MQ=255)
                                                      cAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGa                                                                                                 >  2:801511/1‑150 (MQ=255)
                                                       aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAg                                                                                                >  1:1079760/1‑150 (MQ=255)
                                                       aaaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAg                                                                                                <  2:4547271/150‑1 (MQ=255)
                                                        aaCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAgg                                                                                               >  2:1394968/1‑150 (MQ=255)
                                                         aCATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGa                                                                                              >  1:4452663/1‑150 (MQ=255)
                                                           aTCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATa                                                                                            >  2:3714232/1‑150 (MQ=255)
                                                            tCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaa                                                                                           >  1:2784090/1‑150 (MQ=255)
                                                              aaCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAg                                                                                         >  2:2206340/1‑150 (MQ=255)
                                                               aCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAgg                                                                                        >  2:334382/1‑150 (MQ=255)
                                                               aCAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAgg                                                                                        >  2:3779599/1‑150 (MQ=255)
                                                                cAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGc                                                                                       >  1:1688744/1‑150 (MQ=255)
                                                                  gactGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCCCCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGCGGATAAAGGCGa                                                                                     <  2:1018582/147‑1 (MQ=255)
                                                                    aTGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGc                                                                                   <  1:2240299/150‑1 (MQ=255)
                                                                     tGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGca                                                                                  >  2:403211/1‑150 (MQ=255)
                                                                     tGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGca                                                                                  >  2:2719775/1‑150 (MQ=255)
                                                                      gACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGcac                                                                                 >  2:3397848/1‑150 (MQ=255)
                                                                       aCGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGcaca                                                                                <  1:3091672/150‑1 (MQ=255)
                                                                         gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAg                                                                              <  2:913994/150‑1 (MQ=255)
                                                                           ccAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGgc                                                                            >  1:301558/1‑150 (MQ=255)
                                                                             aGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGgccg                                                                          >  1:45256/1‑150 (MQ=255)
                                                                             aGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGgccg                                                                          <  2:3279502/150‑1 (MQ=255)
                                                                              gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGgccgc                                                                         <  2:1892348/150‑1 (MQ=255)
                                                                               ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGgccgcc                                                                        >  1:3450829/1‑150 (MQ=255)
                                                                                tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCca                                                                       <  1:2750627/150‑1 (MQ=255)
                                                                                tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCca                                                                       >  2:1374144/1‑150 (MQ=255)
                                                                                  aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCcaac                                                                     <  2:1987829/150‑1 (MQ=255)
                                                                                     cAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAt                                                                  <  1:3012982/150‑1 (MQ=255)
                                                                                      aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtg                                                                 >  2:2808141/1‑150 (MQ=255)
                                                                                      aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtg                                                                 >  2:1425042/1‑150 (MQ=255)
                                                                                         tCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtggtg                                                              >  2:2958636/1‑150 (MQ=255)
                                                                                          ccGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtggtgg                                                             >  2:1202973/1‑150 (MQ=255)
                                                                                           cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATggtggt                                                            <  2:3986811/150‑1 (MQ=255)
                                                                                              ggCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGAt                                                         >  2:697719/1‑150 (MQ=255)
                                                                                                cAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATaa                                                       <  2:3468180/150‑1 (MQ=255)
                                                                                                 aaGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAg                                                      >  1:810010/1‑150 (MQ=255)
                                                                                                         tATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCCGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTgg                                              >  1:502723/1‑150 (MQ=255)
                                                                                                         tATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTgg                                              >  2:1010013/1‑150 (MQ=255)
                                                                                                          aTCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGc                                             <  2:2571094/150‑1 (MQ=255)
                                                                                                                      gCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTcc                                 <  2:1269561/150‑1 (MQ=255)
                                                                                                                      gCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTcc                                 <  2:1859127/150‑1 (MQ=255)
                                                                                                                        ccGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGt                               >  1:2642069/1‑150 (MQ=255)
                                                                                                                         cGGAGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGtc                              >  1:1435668/1‑150 (MQ=255)
                                                                                                                            aGTGAATGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTcc                           >  1:3682595/1‑150 (MQ=255)
                                                                                                                               gAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCttt                        >  2:1423817/1‑150 (MQ=255)
                                                                                                                                aaTGGTCCTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCtttt                       <  2:1539710/150‑1 (MQ=255)
                                                                                                                                aaTGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCtttt                       <  1:100530/150‑1 (MQ=255)
                                                                                                                                aaTGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCtttt                       >  1:4017631/1‑150 (MQ=255)
                                                                                                                                    gTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtg                   >  1:2806594/1‑150 (MQ=255)
                                                                                                                                     tCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtgg                  >  2:2335683/1‑150 (MQ=255)
                                                                                                                                       cTGCCAGCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtggtg                >  2:2155461/1‑150 (MQ=255)
                                                                                                                                           cAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTa            >  1:197099/1‑150 (MQ=255)
                                                                                                                                            aGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTAt           >  2:4456640/1‑150 (MQ=255)
                                                                                                                                            aGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTAt           <  2:3639074/150‑1 (MQ=255)
                                                                                                                                             gCACACCCTCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTAtt          <  1:3831352/150‑1 (MQ=255)
                                                                                                                                             gCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTAtt          <  1:1344494/150‑1 (MQ=255)
                                                                                                                                                     gCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCAttt  >  1:3768087/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTT  >  CP009273/498430‑498728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A