Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,721,644 C→A 16.9% N243K (AAC→AAA)  nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,721,6440CA16.9% 897.8 / 108.8 283N243K (AAC→AAA) nemAchromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
Reads supporting (aligned to +/- strand):  ref base C (110/125);  new base A (21/27);  total (131/152)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

TCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCG  >  CP009273/1721495‑1721793
                                                                                                                                                     |                                                                                                                                                     
   ccTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAcaca                                                                                                                                                    <  2:605197/150‑1 (MQ=255)
     ttcttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGa                                                                                                                                                  >  2:2198672/1‑150 (MQ=255)
     ttcttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGa                                                                                                                                                  >  2:2198623/1‑150 (MQ=255)
     ttcttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGa                                                                                                                                                  >  1:2271218/1‑150 (MQ=255)
     ttcttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGa                                                                                                                                                  >  2:882667/1‑150 (MQ=255)
      tcttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGAt                                                                                                                                                 >  2:2266945/1‑150 (MQ=255)
       cttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATa                                                                                                                                                >  2:3447979/1‑150 (MQ=255)
       cttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATa                                                                                                                                                <  1:3537515/150‑1 (MQ=255)
       cttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATa                                                                                                                                                <  1:3338815/150‑1 (MQ=255)
       cttcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATa                                                                                                                                                <  1:1944719/150‑1 (MQ=255)
         tcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAAc                                                                                                                                              <  2:2395491/150‑1 (MQ=255)
         tcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAAc                                                                                                                                              <  2:4360775/150‑1 (MQ=255)
         tcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAAc                                                                                                                                              <  2:639135/150‑1 (MQ=255)
            aaCCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGc                                                                                                                                           >  1:3654780/1‑150 (MQ=255)
              ccATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGccc                                                                                                                                         <  2:206111/150‑1 (MQ=255)
                aTCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGa                                                                                                                                       >  1:4393202/1‑150 (MQ=255)
                aTCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGa                                                                                                                                       >  1:4062928/1‑150 (MQ=255)
                aTCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGa                                                                                                                                       >  1:3370896/1‑150 (MQ=255)
                   gTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATg                                                                                                                                    <  1:2679184/150‑1 (MQ=255)
                    tACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATga                                                                                                                                   >  1:3316481/1‑150 (MQ=255)
                    tACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCAGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATga                                                                                                                                   >  2:171741/1‑150 (MQ=255)
                      ccGATCAGTACGGCGGCAGCGTGTAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaag                                                                                                                                 <  1:3881633/150‑1 (MQ=255)
                      ccGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaag                                                                                                                                 <  1:2046904/150‑1 (MQ=255)
                       cGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaaga                                                                                                                                >  2:2731856/1‑150 (MQ=255)
                            aGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCg                                                                                                                           >  2:245888/1‑150 (MQ=255)
                             gTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGa                                                                                                                          <  2:3239296/150‑1 (MQ=255)
                              tACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGAt                                                                                                                         <  2:3866916/150‑1 (MQ=255)
                               aCGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATg                                                                                                                        <  2:2131297/150‑1 (MQ=255)
                                cggcggCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGc                                                                                                                       <  2:325667/150‑1 (MQ=255)
                                      cAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTa                                                                                                                 >  2:2759844/1‑150 (MQ=255)
                                      cAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTa                                                                                                                 >  1:1567105/1‑150 (MQ=255)
                                       aGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTAt                                                                                                                >  1:2801774/1‑150 (MQ=255)
                                          gTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTg                                                                                                             <  1:2213845/150‑1 (MQ=255)
                                           tGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGa                                                                                                            <  1:3997064/150‑1 (MQ=255)
                                            ggAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGAt                                                                                                           >  1:3477042/1‑150 (MQ=255)
                                              aaaaTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTg                                                                                                         >  1:2115979/1‑150 (MQ=255)
                                                   cgcgCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaacaa                                                                                                    >  1:817085/1‑150 (MQ=255)
                                                   cgcgCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaacaa                                                                                                    >  1:2614642/1‑150 (MQ=255)
                                                    gcgcACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaacaac                                                                                                   <  1:3601440/150‑1 (MQ=255)
                                                    gcgcACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaacaac                                                                                                   >  1:2192878/1‑150 (MQ=255)
                                                    gcgcACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaacaac                                                                                                   >  1:1321253/1‑150 (MQ=255)
                                                     cgcACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACt                                                                                                  <  2:2853583/150‑1 (MQ=255)
                                                       cACGTTTGGTACTGGAAGTGGTCGATGCCGAGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTgg                                                                                                >  1:3226296/1‑150 (MQ=255)
                                                        aCGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTggg                                                                                               <  2:4513958/150‑1 (MQ=255)
                                                          gTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTa                                                                                             <  2:1436961/150‑1 (MQ=255)
                                                           tttGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTaa                                                                                            <  1:3499940/150‑1 (MQ=255)
                                                              ggTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAcg                                                                                         <  2:1115396/150‑1 (MQ=255)
                                                              ggTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAcg                                                                                         <  1:1883154/150‑1 (MQ=255)
                                                              ggTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAcg                                                                                         >  1:276756/1‑150 (MQ=255)
                                                                 aCTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCgg                                                                                      <  2:489318/150‑1 (MQ=255)
                                                                   tGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCa                                                                                    >  2:3608363/1‑150 (MQ=255)
                                                                     gAAGTGGTCGTTGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCAtt                                                                                  <  1:2138854/150‑1 (MQ=255)
                                                                       aGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGc                                                                                <  2:4442184/150‑1 (MQ=255)
                                                                           gTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTAt                                                                            <  1:4066320/150‑1 (MQ=255)
                                                                             cGATGCCGGGATTGAAGAATGGGGTGCCGANCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCt                                                                          >  1:4285986/1‑150 (MQ=255)
                                                                              gATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTg                                                                         <  1:1236157/150‑1 (MQ=255)
                                                                                tGCGGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGTTACTTTCCAGAACACAGATAACGGACCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCa                                                                       <  2:1251065/150‑1 (MQ=255)
                                                                                   cGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCatat                                                                    >  1:3237676/1‑150 (MQ=255)
                                                                                           aagaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAc                                                            >  1:1366961/1‑150 (MQ=255)
                                                                                           aagaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAc                                                            <  2:3057787/150‑1 (MQ=255)
                                                                                            agaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAcc                                                           >  1:4614386/1‑150 (MQ=255)
                                                                                            agaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAcc                                                           >  1:3498887/1‑150 (MQ=255)
                                                                                            agaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAAcc                                                           <  2:3972742/150‑1 (MQ=255)
                                                                                                  gggTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTg                                                     <  1:2874278/150‑1 (MQ=255)
                                                                                                   ggTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCAGTGCTTATCTGCATATGTCAGAACCAGATTgg                                                    <  2:408644/150‑1 (MQ=255)
                                                                                                        cGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCggg                                               >  1:8674/1‑150 (MQ=255)
                                                                                                         gATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCgggg                                              >  2:405396/1‑150 (MQ=255)
                                                                                                           tCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCgggggg                                            <  2:1770827/150‑1 (MQ=255)
                                                                                                           tCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCgggggg                                            >  2:693357/1‑150 (MQ=255)
                                                                                                            cGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGt                                           >  2:2699541/1‑150 (MQ=255)
                                                                                                            cGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGt                                           >  2:382144/1‑150 (MQ=255)
                                                                                                             gCATTGGCATTCGCGTTTCACCACTCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTg                                          <  2:1072502/150‑1 (MQ=255)
                                                                                                             gCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTg                                          >  1:707150/1‑150 (MQ=255)
                                                                                                               aTTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGaa                                        >  2:2603156/1‑150 (MQ=255)
                                                                                                               aTTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAAGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGaa                                        >  2:2687506/1‑150 (MQ=255)
                                                                                                                     tttCGCGTTTAACCATTCGGTTCTTTCCAGAACACATATAACGGCACTAATGATGATGCCTATGCACTGTATCTGATTTAACAACTGGGTAATCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGtat                                  <  1:171741/149‑1 (MQ=255)
                                                                                                                      ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGtata                                 <  1:525193/150‑1 (MQ=255)
                                                                                                                       tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATAc                                <  2:4239838/150‑1 (MQ=255)
                                                                                                                       tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATAc                                <  1:245888/150‑1 (MQ=255)
                                                                                                                       tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATAc                                <  1:3083552/150‑1 (MQ=255)
                                                                                                                           gTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGAt                            <  1:1412673/150‑1 (MQ=255)
                                                                                                                           gTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGAt                            <  1:1131959/150‑1 (MQ=255)
                                                                                                                            tttCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATg                           <  1:1090215/150‑1 (MQ=255)
                                                                                                                            tttCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATg                           <  2:807254/150‑1 (MQ=255)
                                                                                                                            tttCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATg                           >  2:3184193/1‑150 (MQ=255)
                                                                                                                              tCACCAATCGGTACTTTCCAGAACACAGATACCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCg                         >  2:3324864/1‑150 (MQ=255)
                                                                                                                              tCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCg                         >  1:2593963/1‑150 (MQ=255)
                                                                                                                               cACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTGTACTGATGCGt                        <  2:2957352/150‑1 (MQ=255)
                                                                                                                                  cAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTcc                     <  1:3036868/150‑1 (MQ=255)
                                                                                                                                  cAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTcc                     <  1:3015109/150‑1 (MQ=255)
                                                                                                                                  cAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTcc                     <  2:3370896/150‑1 (MQ=255)
                                                                                                                                  cAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTcc                     >  2:3321113/1‑150 (MQ=255)
                                                                                                                                      cGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGa                 >  1:2957119/1‑150 (MQ=255)
                                                                                                                                         tACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaa              <  1:2198672/150‑1 (MQ=255)
                                                                                                                                           cTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAg            >  1:4126558/1‑150 (MQ=255)
                                                                                                                                            tttCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGt           <  2:4288140/150‑1 (MQ=255)
                                                                                                                                            gttCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCAGTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGt           <  2:1992980/149‑1 (MQ=255)
                                                                                                                                             ttCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa          >  1:866530/1‑150 (MQ=255)
                                                                                                                                                cAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgc       <  2:3019468/150‑1 (MQ=255)
                                                                                                                                                  gAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACgcgc     <  2:1549695/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCG  >  CP009273/1721495‑1721793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A