Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,721,644 C→A 40.5% N243K (AAC→AAA)  nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,721,6440CA40.5% 299.6 / 292.5 256N243K (AAC→AAA) nemAchromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
Reads supporting (aligned to +/- strand):  ref base C (80/72);  new base A (44/60);  total (124/132)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 263 total aligned reads displayed.

TCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCG  >  CP009273/1721495‑1721793
                                                                                                                                                     |                                                                                                                                                     
tctcCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAac                                                                                                                                                       >  2:1624963/1‑150 (MQ=255)
         tcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAAc                                                                                                                                              >  1:1290524/1‑150 (MQ=255)
         tcAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAAc                                                                                                                                              <  1:3242876/150‑1 (MQ=255)
          cAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACg                                                                                                                                             >  2:3924890/1‑150 (MQ=255)
          cAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACg                                                                                                                                             <  2:2181647/150‑1 (MQ=255)
           aaaCCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGACTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACgg                                                                                                                                            >  2:495398/1‑150 (MQ=255)
             aCCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGcc                                                                                                                                          <  2:366584/150‑1 (MQ=255)
              ccATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGccc                                                                                                                                         >  1:2948644/1‑150 (MQ=255)
               cATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCg                                                                                                                                        >  1:1852853/1‑150 (MQ=255)
                 tCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGaa                                                                                                                                      <  1:1142227/150‑1 (MQ=255)
                   gTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATg                                                                                                                                    <  2:1108189/150‑1 (MQ=255)
                     aCCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaa                                                                                                                                  >  1:1974773/1‑150 (MQ=255)
                      ccGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATgaag                                                                                                                                 <  2:3270147/150‑1 (MQ=255)
                       cGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaaga                                                                                                                                <  2:3872048/150‑1 (MQ=255)
                       cGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATgaaga                                                                                                                                >  1:3378918/1‑150 (MQ=255)
                        gATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaagaa                                                                                                                               >  2:4112625/1‑150 (MQ=255)
                        gATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATgaagaa                                                                                                                               <  1:3758692/150‑1 (MQ=255)
                           cAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGcc                                                                                                                            <  1:3061307/150‑1 (MQ=255)
                            aGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCg                                                                                                                           <  2:535122/150‑1 (MQ=255)
                              tACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGAt                                                                                                                         <  2:791247/150‑1 (MQ=255)
                              tACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGAt                                                                                                                         >  1:1446721/1‑150 (MQ=255)
                              tACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGAt                                                                                                                         <  1:531807/150‑1 (MQ=255)
                                 ggcggcAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCa                                                                                                                      >  1:1359569/1‑150 (MQ=255)
                                   cggcAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACt                                                                                                                    <  2:3251471/150‑1 (MQ=255)
                                   cggcAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACt                                                                                                                    >  2:4451428/1‑150 (MQ=255)
                                   cggcAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACt                                                                                                                    <  1:842309/150‑1 (MQ=255)
                                      cAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTa                                                                                                                 <  2:2524390/150‑1 (MQ=255)
                                       aGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTAt                                                                                                                >  2:2649208/1‑150 (MQ=255)
                                       aGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTAt                                                                                                                <  1:966314/150‑1 (MQ=255)
                                           tGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGa                                                                                                            >  2:1957525/1‑150 (MQ=255)
                                            ggAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGAt                                                                                                           <  2:897877/150‑1 (MQ=255)
                                               aaaTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGa                                                                                                        <  2:4268961/150‑1 (MQ=255)
                                               aaaTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGa                                                                                                        <  2:1300868/150‑1 (MQ=255)
                                               aaaTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGa                                                                                                        <  1:2434912/150‑1 (MQ=255)
                                                 aTCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGaac                                                                                                      <  1:982962/150‑1 (MQ=255)
                                                      gcACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTg                                                                                                 >  2:3085250/1‑150 (MQ=255)
                                                      gcACGTATGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTg                                                                                                 >  2:376094/1‑150 (MQ=255)
                                                          gTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAATGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTa                                                                                             <  1:2209454/150‑1 (MQ=255)
                                                            ttGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTaaa                                                                                           >  2:2921222/1‑150 (MQ=255)
                                                              ggTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAcg                                                                                         <  2:3863878/150‑1 (MQ=255)
                                                                tACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAcgcg                                                                                       <  2:4485059/150‑1 (MQ=255)
                                                                   tGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCa                                                                                    >  2:2420974/1‑150 (MQ=255)
                                                                   tGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCa                                                                                    >  2:1670607/1‑150 (MQ=255)
                                                                    ggAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCAt                                                                                   <  2:4493118/150‑1 (MQ=255)
                                                                      aaGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTg                                                                                 <  2:1389348/150‑1 (MQ=255)
                                                                      aaGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTg                                                                                 >  2:12440/1‑150 (MQ=255)
                                                                        gTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCt                                                                               <  1:2796799/150‑1 (MQ=255)
                                                                           gTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTAt                                                                            <  2:4151347/150‑1 (MQ=255)
                                                                           gTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTAt                                                                            <  2:62721/150‑1 (MQ=255)
                                                                              gATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTg                                                                         >  2:2857174/1‑150 (MQ=255)
                                                                              gATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTg                                                                         >  2:3209707/1‑150 (MQ=255)
                                                                               aTGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGc                                                                        >  1:3619925/1‑150 (MQ=255)
                                                                                  ccGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCata                                                                     <  1:1581999/150‑1 (MQ=255)
                                                                                  ccGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCata                                                                     <  1:42015/150‑1 (MQ=255)
                                                                                    gggATTGAAGAATGGGGTTCCGATCGCATTGGATTTCGCGTTTCTCCAATCGGTACTTTCCTGAACACTGATATCGGAACGATTGAAGAATCCGATGCACTGTATCTGTTTGAAGAACTGGGTATACGCGGCATTGCTTATCTGCATATg                                                                   <  2:3984673/150‑1 (MQ=255)
                                                                                    gggATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATg                                                                   >  1:3509213/1‑150 (MQ=255)
                                                                                    gggATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATg                                                                   >  1:4232447/1‑150 (MQ=255)
                                                                                     ggATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGt                                                                  >  2:3686737/1‑150 (MQ=255)
                                                                                     ggATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGt                                                                  <  2:2628217/150‑1 (MQ=255)
                                                                                             gaaTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCa                                                          >  2:2523871/1‑150 (MQ=255)
                                                                                               aTGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGa                                                        >  1:1817886/1‑150 (MQ=255)
                                                                                                 ggggTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGAtt                                                      >  1:205798/1‑150 (MQ=255)
                                                                                                 ggggTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGAtt                                                      <  1:463401/150‑1 (MQ=255)
                                                                                                 ggggTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGAtt                                                      >  1:1778406/1‑150 (MQ=255)
                                                                                                     tGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGc                                                  <  2:4548699/150‑1 (MQ=255)
                                                                                                     tGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGc                                                  >  1:2216579/1‑150 (MQ=255)
                                                                                                     tGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGc                                                  <  2:268928/150‑1 (MQ=255)
                                                                                                        cGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCggg                                               >  2:289103/1‑150 (MQ=255)
                                                                                                         gATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCgggg                                              <  2:3446215/150‑1 (MQ=255)
                                                                                                          aTCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCggggg                                             >  2:147547/1‑150 (MQ=255)
                                                                                                           tCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCgggggg                                            <  1:4466572/150‑1 (MQ=255)
                                                                                                            cGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGt                                           <  2:3897834/150‑1 (MQ=255)
                                                                                                             gCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTg                                          >  2:176906/1‑150 (MQ=255)
                                                                                                               aTTGTCATTCGCGTTTCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAAAGCGGCATTGCTTATCTCCATATGTCAGAACAAGAATGGCCGGGGGGTGaa                                        >  2:1730882/1‑150 (MQ=255)
                                                                                                               aTTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGaa                                        <  1:2344560/150‑1 (MQ=255)
                                                                                                               aTTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGaa                                        <  1:2562447/150‑1 (MQ=255)
                                                                                                               aTTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGaa                                        <  1:4011511/150‑1 (MQ=255)
                                                                                                                  ggCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCg                                     >  2:4377449/1‑150 (MQ=255)
                                                                                                                   gCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGt                                    >  1:1266439/1‑150 (MQ=255)
                                                                                                                    cATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGta                                   >  1:697515/1‑150 (MQ=255)
                                                                                                                    cATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGta                                   >  2:2208354/1‑150 (MQ=255)
                                                                                                                    cATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGta                                   <  1:1886009/150‑1 (MQ=255)
                                                                                                                     aTTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGtat                                  >  2:1860086/1‑150 (MQ=255)
                                                                                                                      ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGtata                                 >  1:1936460/1‑150 (MQ=255)
                                                                                                                        cgcgTATCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACt                               <  1:4168153/150‑1 (MQ=255)
                                                                                                                         gcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTg                              <  1:1013453/150‑1 (MQ=255)
                                                                                                                            tttCACCAATCGGTACTTTCCAGAACAAATATAACTGCCCTAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGAATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATg                           <  1:579581/150‑1 (MQ=255)
                                                                                                                            tttCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATg                           >  2:2068207/1‑150 (MQ=255)
                                                                                                                             ttCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGc                          >  1:3876501/1‑150 (MQ=255)
                                                                                                                              tCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCg                         <  1:1417120/150‑1 (MQ=255)
                                                                                                                              tCACCAATCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCg                         <  1:599744/150‑1 (MQ=255)
                                                                                                                                aCCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGtt                       <  2:1290524/150‑1 (MQ=255)
                                                                                                                                 ccAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTc                      <  2:1963792/150‑1 (MQ=255)
                                                                                                                                 ccAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTc                      >  1:4494441/1‑150 (MQ=255)
                                                                                                                                   aaTCGGTACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCcg                    >  2:1637026/1‑150 (MQ=255)
                                                                                                                                         tACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaa              >  1:2722683/1‑150 (MQ=255)
                                                                                                                                         tACTTTCCAGAAAACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaa              >  1:3083044/1‑150 (MQ=255)
                                                                                                                                                  gAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACgcgc     >  2:786038/1‑150 (MQ=255)
                                                                                                                                                   aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGcc    <  1:2728834/150‑1 (MQ=255)
                                                                                                                                                    acacAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGccc   <  1:1714996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTTTGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCG  >  CP009273/1721495‑1721793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A