Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,774,858 C→T 12.7% Y67Y (TAC→TAT)  ydiR → putative electron transfer flavoprotein, FAD‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,774,8580CT12.7% 891.2 / 89.5 290Y67Y (TAC→TAT) ydiRputative electron transfer flavoprotein, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (129/124);  new base T (19/18);  total (148/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 291 total aligned reads displayed.

GCTGAACTGTTTGGCGGCGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATATTGTCG  >  CP009273/1774709‑1775006
                                                                                                                                                     |                                                                                                                                                    
   gAACTGTTTGGCGGCGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGcc                                                                                                                                                   <  1:3810448/150‑1 (MQ=255)
    aaCTGTTTGGCGGCGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCg                                                                                                                                                  >  1:1442110/1‑150 (MQ=255)
    aaCTGTTTGGCGGCGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCg                                                                                                                                                  >  1:2362552/1‑150 (MQ=255)
            ggcggcGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCAtt                                                                                                                                          <  1:3184502/150‑1 (MQ=255)
             gcggcgCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATtg                                                                                                                                         <  2:3736725/150‑1 (MQ=255)
              cggcgCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATtgc                                                                                                                                        <  1:4188759/150‑1 (MQ=255)
               ggcgCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATtgct                                                                                                                                       <  1:2229220/150‑1 (MQ=255)
                    tCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCct                                                                                                                                  <  1:904511/150‑1 (MQ=255)
                      aGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCctgc                                                                                                                                >  1:517164/1‑150 (MQ=255)
                      aGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCctgc                                                                                                                                >  1:3829114/1‑150 (MQ=255)
                      aGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCctgc                                                                                                                                >  1:1011538/1‑150 (MQ=255)
                      aGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCctgc                                                                                                                                >  1:3700031/1‑150 (MQ=255)
                         aaTGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGACTTATGGTCCAAAATGACTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGa                                                                                                                             >  1:3772559/1‑150 (MQ=255)
                                ccAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATaa                                                                                                                      >  2:634424/1‑150 (MQ=255)
                                  aaCAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAac                                                                                                                    <  2:1122040/150‑1 (MQ=255)
                                   aCAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAaca                                                                                                                   <  2:1499691/150‑1 (MQ=255)
                                        tgtATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCg                                                                                                              <  1:676542/150‑1 (MQ=255)
                                          tATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCt                                                                                                            >  1:3127951/1‑150 (MQ=255)
                                          tATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCt                                                                                                            >  1:3868671/1‑150 (MQ=255)
                                          tATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCt                                                                                                            <  1:852438/150‑1 (MQ=255)
                                           aTGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTa                                                                                                           >  1:3806992/1‑150 (MQ=255)
                                                  tGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCt                                                                                                    <  1:774389/150‑1 (MQ=255)
                                                    tACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAAAGTCTTTATGTTCTTGAGCAAAACGACGCGCTGCAACGCACGGAAAATTACGCAGAAAGCATAGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGt                                                                                                  >  1:1487605/1‑150 (MQ=255)
                                                     aCAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGtt                                                                                                 <  2:3567335/150‑1 (MQ=255)
                                                     aCAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGtt                                                                                                 <  2:2668573/150‑1 (MQ=255)
                                                      cAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTg                                                                                                >  1:2342548/1‑150 (MQ=255)
                                                       aaaaTACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTgg                                                                                               >  2:1096491/1‑150 (MQ=255)
                                                        aaaTACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGc                                                                                              >  1:2592259/1‑150 (MQ=255)
                                                         aaTACCGACCCGGCGCAGGCTGTTAGGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCGGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGcc                                                                                             <  1:3146035/150‑1 (MQ=255)
                                                          aTACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCcg                                                                                            <  2:3931425/150‑1 (MQ=255)
                                                          aTACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCcg                                                                                            <  1:3575920/150‑1 (MQ=255)
                                                           tACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAATATAAACACCCCGCTATGTTGCTGTTGGCcgc                                                                                           >  2:2540022/1‑150 (MQ=255)
                                                               gACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGcgacg                                                                                       >  1:2754430/1‑150 (MQ=255)
                                                                aCCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGcgacga                                                                                      <  1:776875/150‑1 (MQ=255)
                                                                   cggCGCAGGCAGTTAGGCCTTATGGTCCAAACTGACTTTATGTTCTTGCGCACAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTAGGTTGCTGTTGGCCGCGACGAAAc                                                                                   <  2:3555855/149‑1 (MQ=255)
                                                                   aGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAAc                                                                                   >  2:2372181/1‑150 (MQ=255)
                                                                   aGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAAc                                                                                   >  1:409026/1‑150 (MQ=255)
                                                                    ggCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACg                                                                                  >  2:319525/1‑150 (MQ=255)
                                                                    ggCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACg                                                                                  >  2:4531899/1‑150 (MQ=255)
                                                                    ggCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGAAGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACg                                                                                  <  2:1838122/150‑1 (MQ=255)
                                                                       gcaggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTgg                                                                               <  2:2489734/150‑1 (MQ=255)
                                                                       gcaggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTgg                                                                               >  2:3560985/1‑150 (MQ=255)
                                                                         aggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTa                                                                             <  2:1373608/150‑1 (MQ=255)
                                                                         aggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTa                                                                             >  1:3243345/1‑150 (MQ=255)
                                                                          ggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTaa                                                                            >  1:17074/1‑150 (MQ=255)
                                                                          ggcagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTaa                                                                            >  1:16904/1‑150 (MQ=255)
                                                                            cagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAg                                                                          <  2:1743763/150‑1 (MQ=255)
                                                                            cagTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAg                                                                          <  2:2306751/150‑1 (MQ=255)
                                                                             agTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTCAAgc                                                                         >  2:1339712/1‑150 (MQ=255)
                                                                             agTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAgc                                                                         >  2:220720/1‑150 (MQ=255)
                                                                             agTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAgc                                                                         <  2:4144085/150‑1 (MQ=255)
                                                                             agTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAgc                                                                         >  2:3218239/1‑150 (MQ=255)
                                                                              gTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAgcg                                                                        <  2:2924085/150‑1 (MQ=255)
                                                                              gTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAgcg                                                                        <  1:2473380/150‑1 (MQ=255)
                                                                                tATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCt                                                                      <  1:555901/150‑1 (MQ=255)
                                                                                    ccTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGgca                                                                  >  1:1122071/1‑150 (MQ=255)
                                                                                    ccTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGgca                                                                  <  1:468430/150‑1 (MQ=255)
                                                                                    ccTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGgca                                                                  >  1:3009328/1‑150 (MQ=255)
                                                                                     cTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATACACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGgcag                                                                 >  2:4328327/1‑150 (MQ=255)
                                                                                      ttATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGgcagc                                                                >  2:332050/1‑150 (MQ=255)
                                                                                         tGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACg                                                             <  1:4559699/150‑1 (MQ=255)
                                                                                            tCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGtg                                                          >  2:1227445/1‑149 (MQ=255)
                                                                                               aaaaTGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACTCACTGAAAATTACTCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCACGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAg                                                       >  2:3923775/1‑150 (MQ=255)
                                                                                                 aaTGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAgtg                                                     >  2:260218/1‑150 (MQ=255)
                                                                                                 aaTGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAgtg                                                     >  2:4092646/1‑150 (MQ=255)
                                                                                                    gTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGc                                                  <  1:4040585/150‑1 (MQ=255)
                                                                                                    gTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGc                                                  >  1:28039/1‑150 (MQ=255)
                                                                                                     tCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCa                                                 <  2:3700031/150‑1 (MQ=255)
                                                                                                     tCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCa                                                 <  2:1011538/150‑1 (MQ=255)
                                                                                                         tATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTg                                             <  2:4018656/150‑1 (MQ=255)
                                                                                                          aTGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGa                                            <  1:1642886/150‑1 (MQ=255)
                                                                                                            gTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAAt                                          >  2:3279874/1‑150 (MQ=255)
                                                                                                            gTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAAt                                          >  2:3279909/1‑150 (MQ=255)
                                                                                                              tCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATgc                                        <  2:468189/150‑1 (MQ=255)
                                                                                                               cTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATgcg                                       <  2:780789/150‑1 (MQ=255)
                                                                                                                 tGCGCAAAACGACGCGCTGCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATgcggc                                     >  2:2049291/1‑150 (MQ=255)
                                                                                                                 tGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATgcggc                                     >  1:4063475/1‑150 (MQ=255)
                                                                                                                       aaaCGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGt                               <  2:4369930/150‑1 (MQ=255)
                                                                                                                       aaaCGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGt                               <  2:802470/150‑1 (MQ=255)
                                                                                                                        aaCGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTg                              >  1:4439523/1‑150 (MQ=255)
                                                                                                                           gacgCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAAc                           >  2:1695553/1‑150 (MQ=255)
                                                                                                                             cgcgCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGa                         >  2:1162758/1‑150 (MQ=255)
                                                                                                                               cgcTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATg                       >  1:1861810/1‑150 (MQ=255)
                                                                                                                                gcTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGc                      >  1:2063304/1‑150 (MQ=255)
                                                                                                                                 cTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGcc                     >  2:3448100/1‑150 (MQ=255)
                                                                                                                                 cTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGcc                     >  2:3091546/1‑150 (MQ=255)
                                                                                                                                  tGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCa                    >  2:1550442/1‑150 (MQ=255)
                                                                                                                                   gCAACGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGACGCGACGATACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCAc                   <  2:3772559/150‑1 (MQ=255)
                                                                                                                                     aaCGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCAcgg                 >  2:1404164/1‑150 (MQ=255)
                                                                                                                                      aCGCACTGAAAATTATGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCAcggc                <  2:1765555/150‑1 (MQ=255)
                                                                                                                                         cACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGt             <  1:1776787/150‑1 (MQ=255)
                                                                                                                                           cTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTgg           <  1:1354458/150‑1 (MQ=255)
                                                                                                                                            tGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGa          <  1:4362057/150‑1 (MQ=255)
                                                                                                                                            tGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGa          >  1:1418709/1‑150 (MQ=255)
                                                                                                                                             gAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGat         <  2:2592259/150‑1 (MQ=255)
                                                                                                                                             gAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGat         <  1:1956817/150‑1 (MQ=255)
                                                                                                                                                aaTTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATAtt      <  2:3829114/150‑1 (MQ=255)
                                                                                                                                                   tACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATATTGTc   <  1:267413/150‑1 (MQ=255)
                                                                                                                                                   tACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATATTGTc   <  1:3418319/150‑1 (MQ=255)
                                                                                                                                                    aCGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATATTGTCg  >  1:653767/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCTGAACTGTTTGGCGGCGCTCAGCAATGGGGCCAACAGGTGTATGCCATTGTACAAAATACCGACCAGGCGCAGGCAGTTATGCCTTATGGTCCAAAATGTCTTTATGTTCTTGCGCAAAACGACGCGCTGCAACGCACTGAAAATTACGCCGAAAGCATTGCTGCCCTGCTGAAAGATAAACACCCCGCTATGTTGCTGTTGGCCGCGACGAAACGTGGTAAAGCGCTGGCAGCACGGTTAAGTGTGCAACTGAATGCGGCGCTGGTGAACGATGCCACGGCGGTGGATATTGTCG  >  CP009273/1774709‑1775006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A