Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,820,020 C→A 18.6% intergenic (‑138/+192) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,820,0200CA18.6% 679.3 / 99.4 225intergenic (‑138/+192)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (91/92);  new base A (16/26);  total (107/118)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 234 total aligned reads displayed.

TAATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAG  >  CP009273/1819871‑1820169
                                                                                                                                                     |                                                                                                                                                      
tAATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTATGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTgc                                                                                                                                                        >  1:2621536/1‑150 (MQ=255)
tAATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTgc                                                                                                                                                        >  2:760143/1‑150 (MQ=255)
tAATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTgc                                                                                                                                                        <  2:3432928/150‑1 (MQ=255)
    ttACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCa                                                                                                                                                    <  1:1964140/150‑1 (MQ=255)
      aCGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAAt                                                                                                                                                  <  2:3201313/150‑1 (MQ=255)
       cGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAAtt                                                                                                                                                 >  2:2026471/1‑150 (MQ=255)
        gCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTa                                                                                                                                                <  1:821156/150‑1 (MQ=255)
          atatTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATg                                                                                                                                              <  2:4392963/150‑1 (MQ=255)
             ttCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGgag                                                                                                                                           <  2:2483431/150‑1 (MQ=255)
              tCTATATCCTTCCTTTCAGTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGgag‑a                                                                                                                                         >  1:3627531/1‑150 (MQ=255)
              tCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTTAGTAGCGTGCCGTGTTGACGAGATTAACTTTCCTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGgag‑a                                                                                                                                         <  1:2625497/150‑1 (MQ=255)
                 atatCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑aaaa                                                                                                                                      >  2:3677197/1‑150 (MQ=255)
                   atCCTTCCTTTCAGTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑aaaaaa                                                                                                                                    >  2:2309535/1‑150 (MQ=255)
                    tccttcctTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑aaaaaaa                                                                                                                                   >  2:2739321/1‑150 (MQ=255)
                     ccttccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAg                                                                                                                                  >  1:2065657/1‑150 (MQ=255)
                      cttccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGa                                                                                                                                 >  1:1205320/1‑150 (MQ=255)
                       ttccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGaa                                                                                                                                >  1:342260/1‑150 (MQ=255)
                          cttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAAt                                                                                                                             <  2:2172339/150‑1 (MQ=255)
                            ttCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAAttt                                                                                                                           <  2:4041909/150‑1 (MQ=255)
                             tCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTg                                                                                                                          <  1:2887291/150‑1 (MQ=255)
                             tCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTg                                                                                                                          <  1:838263/150‑1 (MQ=255)
                               aGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGAt                                                                                                                        >  2:1414624/1‑150 (MQ=255)
                                gTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATg                                                                                                                       >  1:3995775/1‑150 (MQ=255)
                                gTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATg                                                                                                                       >  1:1605709/1‑150 (MQ=255)
                                 ttatttatTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTTCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATgg                                                                                                                      <  1:1740064/150‑1 (MQ=255)
                                 ttatttatTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATgg                                                                                                                      <  2:3442777/150‑1 (MQ=255)
                                   atttattACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGgag                                                                                                                    >  1:36753/1‑150 (MQ=255)
                                     ttattaCGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGagag                                                                                                                  <  1:3041526/150‑1 (MQ=255)
                                      tattaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGt                                                                                                                 <  2:3366337/150‑1 (MQ=255)
                                       attaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGtgtg                                                                                                                >  1:3102470/1‑150 (MQ=255)
                                        ttaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGtga                                                                                                               <  2:2061894/150‑1 (MQ=255)
                                         taCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGtgat                                                                                                              <  1:4207200/150‑1 (MQ=255)
                                         taCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGtgat                                                                                                              <  2:1985378/150‑1 (MQ=255)
                                            ggCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATgag                                                                                                           <  2:1062365/150‑1 (MQ=255)
                                              cTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATgagag                                                                                                         <  2:4608461/150‑1 (MQ=255)
                                              cTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATgagag                                                                                                         <  1:201173/150‑1 (MQ=255)
                                                 tCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAat                                                                                                      <  2:1871780/150‑1 (MQ=255)
                                                  cTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAAGTTGATGGAGAGTGATGAGAGAata                                                                                                     >  1:4637453/1‑150 (MQ=255)
                                                          gCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTacaaca                                                                                             <  2:3130779/150‑1 (MQ=255)
                                                          gCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTacaaca                                                                                             <  1:4306532/150‑1 (MQ=255)
                                                           cGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAacac                                                                                            <  2:803062/150‑1 (MQ=255)
                                                            gTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACg                                                                                           >  1:4342606/1‑150 (MQ=255)
                                                             tGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACga                                                                                          >  2:55909/1‑150 (MQ=255)
                                                              gCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgat                                                                                         <  2:3667592/150‑1 (MQ=255)
                                                               ccGTGTTGACGAGATTAAATTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgatg                                                                                        <  2:4233158/150‑1 (MQ=255)
                                                                cGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgatga                                                                                       <  2:61477/150‑1 (MQ=255)
                                                                    ttGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGAtttt                                                                                   <  1:2058255/150‑1 (MQ=255)
                                                                     tGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTg                                                                                  >  2:1823423/1‑150 (MQ=255)
                                                                     tGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGACAATATTACAACACGATGATTTTg                                                                                  <  1:118359/150‑1 (MQ=255)
                                                                     tGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTGTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTATg                                                                                  >  2:1823339/1‑150 (MQ=255)
                                                                      gACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATGTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGc                                                                                 <  1:2965950/150‑1 (MQ=255)
                                                                        cGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCag                                                                               <  2:2333596/150‑1 (MQ=255)
                                                                            aTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGANAATATTACAACACGATGATTTTGCAGAGAt                                                                           <  1:4278634/150‑1 (MQ=255)
                                                                             ttAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGAtt                                                                          <  1:238505/150‑1 (MQ=255)
                                                                              tAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTa                                                                         <  2:4169966/150‑1 (MQ=255)
                                                                              tAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTa                                                                         <  1:3840190/150‑1 (MQ=255)
                                                                               aaCTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTAt                                                                        >  2:1792807/1‑150 (MQ=255)
                                                                                    tACTGACTGTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATgaag                                                                    >  1:4062897/1‑150 (MQ=255)
                                                                                     aCTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATgaagaa                                                                  <  1:2705389/150‑1 (MQ=255)
                                                                                      cTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAAc                                                                 >  1:2475770/1‑150 (MQ=255)
                                                                                       tGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACt                                                                >  1:4435060/1‑150 (MQ=255)
                                                                                        taCTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTTTAAAAAACACTTTTGCGCCAATTATGTAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGATATTATGAATAACta                                                               <  2:3668087/149‑1 (MQ=255)
                                                                                          cTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACtata                                                             >  1:309180/1‑150 (MQ=255)
                                                                                            ttAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATAcc                                                           <  2:1588219/150‑1 (MQ=255)
                                                                                              aGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCgg                                                         <  1:2732301/150‑1 (MQ=255)
                                                                                                    aaTTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACt                                                   <  1:3170056/150‑1 (MQ=255)
                                                                                                     aTTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTg                                                  >  1:4634037/1‑150 (MQ=255)
                                                                                                      ttAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTgg                                                 <  2:3796231/150‑1 (MQ=255)
                                                                                                       tAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGt                                                <  1:1020920/150‑1 (MQ=255)
                                                                                                       tAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGt                                                >  1:1284064/1‑150 (MQ=255)
                                                                                                        aaTCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTg                                               >  1:4466952/1‑150 (MQ=255)
                                                                                                        aaTCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTg                                               <  2:1934073/150‑1 (MQ=255)
                                                                                                          tCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGat                                             >  1:4098472/1‑150 (MQ=255)
                                                                                                           cAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGata                                            >  2:4555654/1‑150 (MQ=255)
                                                                                                            agagCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGataa                                           <  2:4272635/150‑1 (MQ=255)
                                                                                                                 aaCGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGAtaaataaa                                      <  1:4478681/150‑1 (MQ=255)
                                                                                                                    ggTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCa                                   >  2:658507/1‑150 (MQ=255)
                                                                                                                       aaaaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAAt                                >  2:401485/1‑150 (MQ=255)
                                                                                                                       aaaaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAAt                                <  1:2406311/150‑1 (MQ=255)
                                                                                                                         aaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATaa                              <  1:2213567/150‑1 (MQ=255)
                                                                                                                         aaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATaa                              >  2:2441131/1‑150 (MQ=255)
                                                                                                                          aCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAAc                             >  2:3736285/1‑150 (MQ=255)
                                                                                                                           cAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAAcc                            >  1:1511207/1‑150 (MQ=255)
                                                                                                                            aaTGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCa                           >  2:208633/1‑150 (MQ=255)
                                                                                                                              tGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAgg                         >  2:2219428/1‑150 (MQ=255)
                                                                                                                               gAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGa                        <  2:36695/150‑1 (MQ=255)
                                                                                                                                aaaGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGAt                       >  1:845704/1‑150 (MQ=255)
                                                                                                                                     gtAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATc                  >  2:2523789/1‑150 (MQ=255)
                                                                                                                                      tAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACTGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCt                 <  2:3825886/150‑1 (MQ=255)
                                                                                                                                      tAAAAAACACTTTTGAGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCt                 <  1:2475614/150‑1 (MQ=255)
                                                                                                                                       aaaaaaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTg                >  1:1916916/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTg                >  1:1125010/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTg                >  1:1124859/1‑150 (MQ=255)
                                                                                                                                         aaaaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTa              >  1:134677/1‑150 (MQ=255)
                                                                                                                                           aaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTAtt            >  2:1227805/1‑150 (MQ=255)
                                                                                                                                           aaCACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTAtt            >  1:3704900/1‑150 (MQ=255)
                                                                                                                                                 tttGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTta      <  2:2786411/150‑1 (MQ=255)
                                                                                                                                                 tttGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTta      >  2:1491730/1‑150 (MQ=255)
                                                                                                                                                  ttGCGCCAATTATGGAG‑AAAAAAAGAAACTTTGATGGCGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTtat     <  2:3427318/150‑1 (MQ=255)
                                                                                                                                                    gcgcCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATaa   >  1:3569933/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TAATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAG‑AAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAG  >  CP009273/1819871‑1820169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A