Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,820,020	C→A	43.9%	intergenic (‑138/+192)	spy ← / ← astE	periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,820,020	0	C	A	43.9%	270.4 / inf	281	intergenic (‑138/+192)	spy/astE	periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand): ref base C (78/79); new base A (63/61); total (141/140)
Fisher's exact test for biased strand distribution p-value = 9.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

AATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAG  >  CP009273/1819872‑1820169

aaTTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTgcg                                                                                                                                                      <  2:1440665/150‑1 (MQ=255)

  tttACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGcc                                                                                                                                                    <  2:1201637/150‑1 (MQ=255)

  tttACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGcc                                                                                                                                                    <  2:2435737/150‑1 (MQ=255)

   ttACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCa                                                                                                                                                   <  1:4526931/150‑1 (MQ=255)

   ttACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCa                                                                                                                                                   <  1:967623/150‑1 (MQ=255)

     aCGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAAt                                                                                                                                                 >  1:955936/1‑150 (MQ=255)

      cGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAAtt                                                                                                                                                >  2:891164/1‑150 (MQ=255)

         atatTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATg                                                                                                                                             <  1:772371/150‑1 (MQ=255)

          tatTCTATATCCTTCCTTTCAGTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATgg                                                                                                                                            <  1:2986541/150‑1 (MQ=255)

            ttCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGgag                                                                                                                                          <  1:3412527/150‑1 (MQ=255)

                atatCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGaaaa                                                                                                                                      >  2:2394422/1‑150 (MQ=255)

                  atCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGaaaaaa                                                                                                                                    >  1:132323/1‑150 (MQ=255)

                  atCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGaaaaaa                                                                                                                                    >  2:3886718/1‑150 (MQ=255)

                   tccttcctTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGaaaaaaa                                                                                                                                   >  2:2196339/1‑150 (MQ=255)

                    ccttccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAg                                                                                                                                  <  2:3531563/150‑1 (MQ=255)

                       tccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGaaa                                                                                                                               <  1:2762528/150‑1 (MQ=255)

                        ccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGaaaa                                                                                                                              <  1:1375234/150‑1 (MQ=255)

                        ccttTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGaaaa                                                                                                                              <  1:4212341/150‑1 (MQ=255)

                          tttCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAAtt                                                                                                                            <  1:659182/150‑1 (MQ=255)

                          tttCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAAtt                                                                                                                            >  2:3932082/1‑150 (MQ=255)

                           ttCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAAttt                                                                                                                           <  2:2609797/150‑1 (MQ=255)

                            tCACTTATTTATTACGGTTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTg                                                                                                                          <  1:1994291/150‑1 (MQ=255)

                              aGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGAt                                                                                                                        >  1:915271/1‑150 (MQ=255)

                                ttatttatTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATgg                                                                                                                      <  2:1521426/150‑1 (MQ=255)

                                 tatttattACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAAAGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGga                                                                                                                     >  2:2700082/1‑150 (MQ=255)

                                  atttattACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGgag                                                                                                                    <  1:2088935/150‑1 (MQ=255)

                                   tttattACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGgaga                                                                                                                   <  2:467082/150‑1 (MQ=255)

                                    ttattaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGagag                                                                                                                  <  1:1679817/150‑1 (MQ=255)

                                    ttattaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGagag                                                                                                                  <  1:1868596/150‑1 (MQ=255)

                                     tattaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGt                                                                                                                 <  1:4460965/150‑1 (MQ=255)

                                     tattaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGt                                                                                                                 >  2:3049062/1‑150 (MQ=255)

                                      attaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGtg                                                                                                                >  1:3641045/1‑150 (MQ=255)

                                      attaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGtg                                                                                                                >  2:2324250/1‑150 (MQ=255)

                                       ttaCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTGTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATAATGGAGAAAAAAAGAAAATTTGATGGAGAGtga                                                                                                               >  1:2895702/1‑150 (MQ=255)

                                        taCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGtgat                                                                                                              >  2:548499/1‑150 (MQ=255)

                                         aCGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGtgatg                                                                                                             <  2:3941118/150‑1 (MQ=255)

                                          cGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGtgatga                                                                                                            >  2:4580106/1‑150 (MQ=255)

                                            gCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATgaga                                                                                                          <  2:1094646/150‑1 (MQ=255)

                                                tCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAat                                                                                                      <  2:4557554/150‑1 (MQ=255)

                                                    aaGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTa                                                                                                  >  1:3978688/1‑150 (MQ=255)

                                                        aGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTacaac                                                                                              <  2:504653/150‑1 (MQ=255)

                                                          cGTGCCGTGTTGACGAGATTAACTTTCCTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAacac                                                                                            <  2:1882891/150‑1 (MQ=255)

                                                             gCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgat                                                                                         <  1:3733637/150‑1 (MQ=255)

                                                              ccGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgatg                                                                                        <  2:1517148/150‑1 (MQ=255)

                                                               cGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgatga                                                                                       >  2:4336378/1‑150 (MQ=255)

                                                                gtgtTTACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACgatgat                                                                                      >  2:3749659/1‑150 (MQ=255)

                                                                 tgtTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGAtt                                                                                     >  2:1380627/1‑150 (MQ=255)

                                                                   ttGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGAtttt                                                                                   <  1:1262977/150‑1 (MQ=255)

                                                                   ttGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGAtttt                                                                                   >  1:4062568/1‑150 (MQ=255)

                                                                     gACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAATAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGc                                                                                 >  1:1446745/1‑150 (MQ=255)

                                                                         agaTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCagag                                                                             <  1:79613/150‑1 (MQ=255)

                                                                         agaTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCagag                                                                             <  2:77696/150‑1 (MQ=255)

                                                                         agaTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCagag                                                                             >  1:2707676/1‑150 (MQ=255)

                                                                         agaTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCagag                                                                             >  2:1175866/1‑150 (MQ=255)

                                                                         agaTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTAGGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCagag                                                                             >  1:3897393/1‑150 (MQ=255)

                                                                           aTTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGAt                                                                           >  2:2121469/1‑150 (MQ=255)

                                                                               aCTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATg                                                                       >  1:1010308/1‑150 (MQ=255)

                                                                                cTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATga                                                                      >  1:4381369/1‑150 (MQ=255)

                                                                                cTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATga                                                                      <  1:4033037/150‑1 (MQ=255)

                                                                                cTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATga                                                                      >  1:3680951/1‑150 (MQ=255)

                                                                                     cTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAAc                                                                 >  2:3343573/1‑150 (MQ=255)

                                                                                      tGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACt                                                                >  1:3942974/1‑150 (MQ=255)

                                                                                       gACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTGTGAAGAACta                                                               <  1:1410036/150‑1 (MQ=255)

                                                                                          tttAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATAc                                                            <  1:938956/150‑1 (MQ=255)

                                                                                           ttAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATAcc                                                           <  1:1146377/150‑1 (MQ=255)

                                                                                               cGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGAt                                                       >  2:1219988/1‑150 (MQ=255)

                                                                                                gTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATg                                                      <  1:1077343/150‑1 (MQ=255)

                                                                                                  cAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGAc                                                    <  1:2196339/150‑1 (MQ=255)

                                                                                                   aaTTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACt                                                   >  1:3874418/1‑150 (MQ=255)

                                                                                                     ttAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTgg                                                 <  2:252578/150‑1 (MQ=255)

                                                                                                      tAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGt                                                <  1:1795735/150‑1 (MQ=255)

                                                                                                       aaTCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTg                                               >  2:3809811/1‑150 (MQ=255)

                                                                                                       aaTCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTg                                               >  2:3809904/1‑150 (MQ=255)

                                                                                                        aTCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGa                                              <  1:4256316/150‑1 (MQ=255)

                                                                                                         tCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGTAGAGATTATGAAGAACTATACCGGATGACTGGTGat                                             <  1:4633147/150‑1 (MQ=255)

                                                                                                          cAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGata                                            >  2:1071786/1‑150 (MQ=255)

                                                                                                           agagCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGataa                                           <  1:4169797/150‑1 (MQ=255)

                                                                                                           agagCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAATAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGataa                                           >  2:3180625/1‑150 (MQ=255)

                                                                                                            gagCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGataaa                                          >  2:1668803/1‑150 (MQ=255)

                                                                                                             agCAACGGTAAAACAATGAAAGTGTAAAAAACAATTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGataaat                                         <  2:4283627/150‑1 (MQ=255)

                                                                                                                     tAAAACAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCaaa                                 <  2:2029799/150‑1 (MQ=255)

                                                                                                                      aaaaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAAt                                >  2:624654/1‑150 (MQ=255)

                                                                                                                      aaaaCAATGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAAt                                <  2:1479553/150‑1 (MQ=255)

                                                                                                                       aaaCAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATa                               <  1:1494592/150‑1 (MQ=255)

                                                                                                                           aaTGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCa                           >  2:4447903/1‑150 (MQ=255)

                                                                                                                            aTGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAg                          >  1:2043273/1‑150 (MQ=255)

                                                                                                                            aTGAAAGTGTAAAAAACACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAg                          >  1:2459302/1‑150 (MQ=255)

                                                                                                                               aaaGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGAt                       <  1:3111867/150‑1 (MQ=255)

                                                                                                                               aaaGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGAt                       >  2:1713888/1‑150 (MQ=255)

                                                                                                                                      aaaaaaCACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTg                <  2:1480411/150‑1 (MQ=255)

                                                                                                                                       aaaaaCACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGt               <  2:3782100/150‑1 (MQ=255)

                                                                                                                                       aaaaaCACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGt               <  2:4057988/150‑1 (MQ=255)

                                                                                                                                       aaaaaCACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGt               >  2:2351083/1‑150 (MQ=255)

                                                                                                                                        aaaaCACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTa              <  2:1016661/150‑1 (MQ=255)

                                                                                                                                         aaaaACTTTTGAGCAAATTATGTATAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTAt             <  2:3262653/150‑1 (MQ=255)

                                                                                                                                          aaCACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTAtt            >  1:2587992/1‑150 (MQ=255)

                                                                                                                                          aaCACTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTAtt            >  1:4363012/1‑150 (MQ=255)

                                                                                                                                              cTTTTGAGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAAtt        >  1:2397931/1‑150 (MQ=255)

                                                                                                                                                tttGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTta      <  1:2242257/150‑1 (MQ=255)

                                                                                                                                                 ttGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTtat     <  2:3388199/150‑1 (MQ=255)

AATTTACGCATATTCTATATCCTTCCTTTCAGTTATTTATTACGGCTTTCTTAAGTAGCGTGCCGTGTTGACGAGATTAACTTTACTGACTTTAGCGTCAATTAATCAGAGCAACGGTAAAACAATGAAAGTGTAAAAAACACTTTTGCGCCAATTATGGAGAAAAAAAGAAAATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAG  >  CP009273/1819872‑1820169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A