Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,948,050 C→A 5.6% L118I (CTT→ATT)  cmoB → tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,948,0500CA 5.6% 1139.4 / 26.3 293L118I (CTT→ATT) cmoBtRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (133/143);  new base A (10/7);  total (143/150)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

AGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTA  >  CP009273/1947902‑1948199
                                                                                                                                                    |                                                                                                                                                     
agagCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCt                                                                                                                                                      <  1:4077230/150‑1 (MQ=255)
 gagCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCtt                                                                                                                                                     <  2:100228/150‑1 (MQ=255)
  agCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCttt                                                                                                                                                    <  1:3698661/150‑1 (MQ=255)
    ccACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCt                                                                                                                                                  <  1:3071623/150‑1 (MQ=255)
        tGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGAtt                                                                                                                                              <  1:216921/150‑1 (MQ=255)
         gAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGAttt                                                                                                                                             <  1:3371395/150‑1 (MQ=255)
             gcAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAAcc                                                                                                                                         >  2:242489/1‑150 (MQ=255)
               aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                       >  1:1402999/1‑150 (MQ=255)
               aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                       <  2:1869632/150‑1 (MQ=255)
               aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                       <  2:2797538/150‑1 (MQ=255)
               aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                       >  1:2262616/1‑150 (MQ=255)
                gggCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGa                                                                                                                                      >  2:373261/1‑150 (MQ=255)
                    aaaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCa                                                                                                                                  <  1:1801942/150‑1 (MQ=255)
                    aaaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCa                                                                                                                                  >  1:1285186/1‑150 (MQ=255)
                       ttAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCa                                                                                                                               >  2:2604997/1‑150 (MQ=255)
                       ttAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCa                                                                                                                               >  2:2851133/1‑150 (MQ=255)
                         aaaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCAtt                                                                                                                             >  2:3709923/1‑150 (MQ=255)
                         aaaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCAtt                                                                                                                             >  2:1385504/1‑150 (MQ=255)
                         aaaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCAtt                                                                                                                             <  1:4352708/150‑1 (MQ=255)
                         aaaCGCATTGAAACGCTGAGGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCAtt                                                                                                                             <  2:2375056/150‑1 (MQ=255)
                          aaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTc                                                                                                                            <  2:3851642/150‑1 (MQ=255)
                            cGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTg                                                                                                                          >  2:2234952/1‑150 (MQ=255)
                             gCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTgg                                                                                                                         >  2:3984777/1‑150 (MQ=255)
                               aTTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGAt                                                                                                                       >  2:3904457/1‑150 (MQ=255)
                               aTTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGAt                                                                                                                       >  2:503578/1‑150 (MQ=255)
                                ttGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATg                                                                                                                      >  2:2584397/1‑150 (MQ=255)
                                 tGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGt                                                                                                                     <  1:2432156/150‑1 (MQ=255)
                                   aaaCGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCg                                                                                                                   >  2:4221298/1‑150 (MQ=255)
                                    aaCGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCgg                                                                                                                  >  1:4420300/1‑150 (MQ=255)
                                       gCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTg                                                                                                               <  1:2877531/150‑1 (MQ=255)
                                            tGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGACATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCa                                                                                                          >  2:1964985/1‑150 (MQ=255)
                                            tGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCa                                                                                                          <  1:634115/150‑1 (MQ=255)
                                            tGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCa                                                                                                          >  1:4512897/1‑150 (MQ=255)
                                              cgcAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGc                                                                                                        >  2:301757/1‑150 (MQ=255)
                                              cgcAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGc                                                                                                        >  2:3304559/1‑150 (MQ=255)
                                                           cGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACAtgt                                                                                           >  2:2290834/1‑150 (MQ=255)
                                                             tGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTgg                                                                                         >  2:1662551/1‑150 (MQ=255)
                                                               gcgcAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGgcg                                                                                       <  1:2903628/150‑1 (MQ=255)
                                                               gcgcAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGgcg                                                                                       <  1:1018363/150‑1 (MQ=255)
                                                               gcgcAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGgcg                                                                                       <  1:1018570/150‑1 (MQ=255)
                                                                  cAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCAt                                                                                    >  1:2575082/1‑150 (MQ=255)
                                                                    aaGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGa                                                                                  >  1:3988806/1‑150 (MQ=255)
                                                                    aaGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGa                                                                                  >  2:972228/1‑150 (MQ=255)
                                                                     aGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGAt                                                                                 >  2:4547619/1‑150 (MQ=255)
                                                                      gggCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGAtt                                                                                <  1:1064596/150‑1 (MQ=255)
                                                                        gCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTgg                                                                              <  1:1972167/150‑1 (MQ=255)
                                                                         ccGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGgc                                                                             <  1:3818275/150‑1 (MQ=255)
                                                                         ccGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGgc                                                                             <  2:1331205/150‑1 (MQ=255)
                                                                          cGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGgcg                                                                            <  1:503578/150‑1 (MQ=255)
                                                                             tctcACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAg                                                                         <  2:1716658/150‑1 (MQ=255)
                                                                               tcACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgc                                                                       <  1:4152823/150‑1 (MQ=255)
                                                                                  cTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgcgcg                                                                    <  2:4420300/150‑1 (MQ=255)
                                                                                   tGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgcgcgc                                                                   >  1:2705858/1‑150 (MQ=255)
                                                                                     tATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCAc                                                                 >  1:162353/1‑150 (MQ=255)
                                                                                      aTGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCAcc                                                                <  1:4221441/150‑1 (MQ=255)
                                                                                       tGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCt                                                               <  2:1325712/150‑1 (MQ=255)
                                                                                       tGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCt                                                               <  1:3794749/150‑1 (MQ=255)
                                                                                        ggCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGGTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTc                                                              <  2:1578410/150‑1 (MQ=255)
                                                                                        ggCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTc                                                              >  1:4172477/1‑150 (MQ=255)
                                                                                          cGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTcgc                                                            >  2:1642511/1‑150 (MQ=255)
                                                                                             cAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGt                                                         >  2:3447973/1‑150 (MQ=255)
                                                                                              aaCATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTg                                                        >  1:4266807/1‑150 (MQ=255)
                                                                                              aaCATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTg                                                        <  2:189058/150‑1 (MQ=255)
                                                                                                cATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTggg                                                      <  1:3458856/150‑1 (MQ=255)
                                                                                                cATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCACGCGGTggg                                                      <  2:2553540/150‑1 (MQ=255)
                                                                                                 aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                     >  2:2029405/1‑150 (MQ=255)
                                                                                                 aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                     >  1:3353399/1‑150 (MQ=255)
                                                                                                 aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGACGGCTGCGCCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                     >  1:541412/1‑150 (MQ=255)
                                                                                                  tCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGa                                                    >  2:4356127/1‑150 (MQ=255)
                                                                                                  tCGAGACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGa                                                    <  2:3571130/150‑1 (MQ=255)
                                                                                                    gATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATc                                                  <  1:3145151/150‑1 (MQ=255)
                                                                                                    gATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATc                                                  <  1:2038209/150‑1 (MQ=255)
                                                                                                     aTACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCg                                                 <  2:2111281/150‑1 (MQ=255)
                                                                                                         cGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGAccc                                             <  1:3505381/150‑1 (MQ=255)
                                                                                                          gAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCt                                            >  2:685575/1‑150 (MQ=255)
                                                                                                          gAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCt                                            <  1:2287723/150‑1 (MQ=255)
                                                                                                            aTGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTAc                                          <  2:903503/150‑1 (MQ=255)
                                                                                                            aTGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTAc                                          <  2:3486845/150‑1 (MQ=255)
                                                                                                             tGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACg                                         <  1:2112296/150‑1 (MQ=255)
                                                                                                              ggCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGc                                        >  2:106006/1‑150 (MQ=255)
                                                                                                              ggCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGc                                        >  1:3576865/1‑150 (MQ=255)
                                                                                                                  ttCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACt                                    <  2:148079/150‑1 (MQ=255)
                                                                                                                  ttCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACt                                    <  1:4120058/150‑1 (MQ=255)
                                                                                                                      gACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGttt                                <  2:4060762/150‑1 (MQ=255)
                                                                                                                       aCTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTc                               >  1:91036/1‑150 (MQ=255)
                                                                                                                        cTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCt                              >  1:3532204/1‑150 (MQ=255)
                                                                                                                        cTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCt                              >  2:4619719/1‑150 (MQ=255)
                                                                                                                             aaTGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCACCTGTTTCTGTGcc                         >  1:76643/1‑150 (MQ=255)
                                                                                                                                 ggATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGtt                     <  2:3414410/150‑1 (MQ=255)
                                                                                                                                    tCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGa                  >  1:3627454/1‑150 (MQ=255)
                                                                                                                                    tCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGa                  <  2:2125702/150‑1 (MQ=255)
                                                                                                                                       tgtTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGc               >  1:966320/1‑150 (MQ=255)
                                                                                                                                            tGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGc          >  1:1192873/1‑150 (MQ=255)
                                                                                                                                               cccATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTa       >  2:3728816/1‑150 (MQ=255)
                                                                                                                                                 cATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTaaa     >  2:2925238/1‑150 (MQ=255)
                                                                                                                                                  aTCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAac    <  1:1524342/150‑1 (MQ=255)
                                                                                                                                                  aTCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAac    <  2:2724007/150‑1 (MQ=255)
                                                                                                                                                  aTCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAac    <  1:261237/150‑1 (MQ=255)
                                                                                                                                                  aTCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAac    <  1:2359083/150‑1 (MQ=255)
                                                                                                                                                    cTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAacta  <  1:1683485/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTA  >  CP009273/1947902‑1948199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A