Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,948,050 C→A 20.9% L118I (CTT→ATT)  cmoB → tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,948,0500CA20.9% 703.2 / 143.7 263L118I (CTT→ATT) cmoBtRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (120/88);  new base A (29/26);  total (149/114)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 268 total aligned reads displayed.

AAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACT  >  CP009273/1947901‑1948198
                                                                                                                                                     |                                                                                                                                                    
aaGAGCCACTGAGCGCAGGGCAAATAAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCAGATCACACAGTATGTCTTCAACAACGATAACGAAAGGCGTTCAGACTGGAAAAGGGATAGTGTTCTGCCCCATc                                                                                                                                                      >  1:4182530/1‑150 (MQ=255)
 agagCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCt                                                                                                                                                     >  1:2950846/1‑150 (MQ=255)
  gagCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCtt                                                                                                                                                    >  2:3468299/1‑150 (MQ=255)
  gagCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCtt                                                                                                                                                    <  2:2101873/150‑1 (MQ=255)
    gCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTc                                                                                                                                                  <  2:1935469/150‑1 (MQ=255)
     ccACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCt                                                                                                                                                 <  2:205572/150‑1 (MQ=255)
     ccACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCt                                                                                                                                                 <  2:116452/150‑1 (MQ=255)
     ccACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCt                                                                                                                                                 <  2:2990091/150‑1 (MQ=255)
      cACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTg                                                                                                                                                <  2:4136435/150‑1 (MQ=255)
      cACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTg                                                                                                                                                >  2:1069897/1‑150 (MQ=255)
      cACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTg                                                                                                                                                >  1:2749628/1‑150 (MQ=255)
      cACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTg                                                                                                                                                >  2:322794/1‑150 (MQ=255)
      cACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTg                                                                                                                                                >  2:1322437/1‑150 (MQ=255)
          gAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGAttt                                                                                                                                            <  1:245142/150‑1 (MQ=255)
           aGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTa                                                                                                                                           <  2:3741415/150‑1 (MQ=255)
             cgcAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAAc                                                                                                                                         >  1:2847923/1‑150 (MQ=255)
              gcAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAAcc                                                                                                                                        <  1:739000/150‑1 (MQ=255)
                aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                      >  2:3743117/1‑150 (MQ=255)
                aGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCgg                                                                                                                                      <  2:405055/150‑1 (MQ=255)
                 gggCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGa                                                                                                                                     >  1:1850162/1‑150 (MQ=255)
                  ggCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGAc                                                                                                                                    <  1:1604062/150‑1 (MQ=255)
                  ggCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGAc                                                                                                                                    <  1:603468/150‑1 (MQ=255)
                     aaaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCa                                                                                                                                 >  2:3558503/1‑150 (MQ=255)
                     aaaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCa                                                                                                                                 >  2:327194/1‑150 (MQ=255)
                     aaaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCa                                                                                                                                 >  2:2304131/1‑150 (MQ=255)
                      aaTTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGACATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCAc                                                                                                                                >  2:1696519/1‑150 (MQ=255)
                        ttAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCa                                                                                                                              >  2:188724/1‑150 (MQ=255)
                        ttAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCa                                                                                                                              >  2:2319454/1‑150 (MQ=255)
                         tAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCAt                                                                                                                             >  2:2024491/1‑150 (MQ=255)
                         tAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCAt                                                                                                                             >  2:2192202/1‑150 (MQ=255)
                           aaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTc                                                                                                                           <  2:4033450/150‑1 (MQ=255)
                           aaCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTc                                                                                                                           >  2:849512/1‑150 (MQ=255)
                            aCGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCt                                                                                                                          >  1:2505382/1‑150 (MQ=255)
                              gCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTgg                                                                                                                        >  1:4526378/1‑150 (MQ=255)
                              gCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTgg                                                                                                                        >  1:3806239/1‑150 (MQ=255)
                                   gAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTc                                                                                                                   >  1:2023375/1‑150 (MQ=255)
                                    aaaCGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCg                                                                                                                  <  2:103232/150‑1 (MQ=255)
                                    aaaCGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCg                                                                                                                  <  1:3343372/150‑1 (MQ=255)
                                         cTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGc                                                                                                             >  1:3974006/1‑150 (MQ=255)
                                           gATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCgg                                                                                                           >  2:3066693/1‑150 (MQ=255)
                                           gATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCgg                                                                                                           <  1:3772355/150‑1 (MQ=255)
                                            aTGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGc                                                                                                          >  2:3778305/1‑150 (MQ=255)
                                            aTGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGc                                                                                                          >  1:3428414/1‑150 (MQ=255)
                                             tGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCa                                                                                                         <  1:1395008/150‑1 (MQ=255)
                                             tGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCa                                                                                                         <  2:3709491/150‑1 (MQ=255)
                                                  aaCCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTACGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTATGATTTAACCGGACGCACCAATCTGGATGTCGGCTGCGGCAGCGGt                                                                                                    >  2:2341722/1‑150 (MQ=255)
                                                     cTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTCACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAt                                                                                                 >  2:715434/1‑150 (MQ=255)
                                                     cTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAt                                                                                                 >  1:3257426/1‑150 (MQ=255)
                                                     cTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTAt                                                                                                 >  2:4241152/1‑150 (MQ=255)
                                                      tGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGTTGCGGCAGCGGTTATc                                                                                                >  2:4693781/1‑150 (MQ=255)
                                                               ggCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGgc                                                                                       <  1:788461/150‑1 (MQ=255)
                                                                 cgcAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGgcgc                                                                                     >  2:1440731/1‑150 (MQ=255)
                                                                    aaaGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATg                                                                                  >  1:681199/1‑150 (MQ=255)
                                                                     aaGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGa                                                                                 <  2:560228/150‑1 (MQ=255)
                                                                       gggCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTATGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGAtt                                                                               >  2:1907600/1‑150 (MQ=255)
                                                                         gCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTgg                                                                             <  2:667337/150‑1 (MQ=255)
                                                                            gTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGgcgc                                                                          >  2:3602903/1‑150 (MQ=255)
                                                                             ttCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCa                                                                         <  2:4450506/150‑1 (MQ=255)
                                                                              tctcACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAg                                                                        <  1:4646873/150‑1 (MQ=255)
                                                                                  aCTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgcgc                                                                    >  1:4307224/1‑150 (MQ=255)
                                                                                    tGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgcgcgc                                                                  <  2:2351218/150‑1 (MQ=255)
                                                                                    tGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGgcgcgc                                                                  <  2:2154455/150‑1 (MQ=255)
                                                                                         ggCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTc                                                             <  2:267188/150‑1 (MQ=255)
                                                                                            gTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTcgcg                                                          >  1:3014989/1‑150 (MQ=255)
                                                                                              cAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGt                                                        <  2:2980015/150‑1 (MQ=255)
                                                                                                aCATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgg                                                      <  1:1877078/150‑1 (MQ=255)
                                                                                                  aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                    >  1:1201897/1‑150 (MQ=255)
                                                                                                  aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                    >  1:1201225/1‑150 (MQ=255)
                                                                                                  aTCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTgggg                                                    >  1:4784139/1‑150 (MQ=255)
                                                                                                   tcgagcccGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGa                                                   <  2:3623078/144‑1 (MQ=255)
                                                                                                   tCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGa                                                   >  2:3160938/1‑150 (MQ=255)
                                                                                                    cGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGAt                                                  <  2:3930467/150‑1 (MQ=255)
                                                                                                     gATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATc                                                 >  1:3590355/1‑150 (MQ=255)
                                                                                                            aaTGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTa                                          <  1:612042/150‑1 (MQ=255)
                                                                                                              tGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACg                                        <  1:151842/150‑1 (MQ=255)
                                                                                                                 cGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCaa                                     >  1:4145905/1‑150 (MQ=255)
                                                                                                                 cGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCaa                                     >  2:1341568/1‑150 (MQ=255)
                                                                                                                 cGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCaa                                     >  1:901378/1‑150 (MQ=255)
                                                                                                                  gTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAAc                                    >  1:2117528/1‑150 (MQ=255)
                                                                                                                  gTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAAc                                    >  1:1936944/1‑150 (MQ=255)
                                                                                                                  gTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAAc                                    >  2:1727160/1‑150 (MQ=255)
                                                                                                                  gTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAAc                                    <  2:2465003/150‑1 (MQ=255)
                                                                                                                      cGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGtt                                <  2:2751854/150‑1 (MQ=255)
                                                                                                                      cGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGtt                                <  2:4526378/150‑1 (MQ=255)
                                                                                                                      cGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGtt                                <  2:3806239/150‑1 (MQ=255)
                                                                                                                      cGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGtt                                <  1:4062125/150‑1 (MQ=255)
                                                                                                                          tGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATAACATGTGGAGCATGATTGGAGCAGGCGCGCACCTCTCGGTGTGGATCGACCCTAAGCAACTGTTTCtg                            >  2:1570630/1‑150 (MQ=255)
                                                                                                                           ggAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCtgt                           >  1:2069524/1‑150 (MQ=255)
                                                                                                                              aaTGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCACCTGTTTCTGTGcc                        >  1:422950/1‑150 (MQ=255)
                                                                                                                                tGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAg                      >  2:3075960/1‑150 (MQ=255)
                                                                                                                                tGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAg                      >  2:2562602/1‑150 (MQ=255)
                                                                                                                                tGGGATCGTGTTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAg                      >  2:4398050/1‑150 (MQ=255)
                                                                                                                                        tgtTCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGc              <  2:3015692/150‑1 (MQ=255)
                                                                                                                                         gtTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCg             <  2:1213338/150‑1 (MQ=255)
                                                                                                                                           tCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGt           <  2:2747184/150‑1 (MQ=255)
                                                                                                                                           tCTGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGt           <  2:2835618/150‑1 (MQ=255)
                                                                                                                                             tGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGc         >  2:947/1‑150 (MQ=255)
                                                                                                                                             tGCCCCATATTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGc         <  2:4341020/150‑1 (MQ=255)
                                                                                                                                              gCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCg        <  1:3753248/150‑1 (MQ=255)
                                                                                                                                                    tCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAact  <  1:426467/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGAGCCACTGAGCGCAGGGCAAATTAAACGCATTGAAACGCTGATGCGCAACCTGATGCCGTGGCGCAAAGGGCCGTTCTCACTGTATGGCGTCAACATCGATACCGAATGGCGTTCCGACTGGAAATGGGATCGTGTTCTGCCCCATCTTTCTGATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACT  >  CP009273/1947901‑1948198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A