Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,969,291 G→T 100% I97I (ATC→ATA)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,969,2910GT97.3% 734.9 / 10.2 223I97I (ATC→ATA) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (107/110);  total (110/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGA  >  CP009273/1969144‑1969437
                                                                                                                                                   |                                                                                                                                                  
tttGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattat                                                                                                                                                  <  1:3945618/150‑1 (MQ=255)
 ttGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattatg                                                                                                                                                 <  1:311686/150‑1 (MQ=255)
 ttGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattatg                                                                                                                                                 <  1:2836912/150‑1 (MQ=255)
  tGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGt                                                                                                                                                >  2:4089249/1‑150 (MQ=255)
      aaCCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCtt                                                                                                                                            <  2:3491263/150‑1 (MQ=255)
        ccACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCg                                                                                                                                          <  2:4334519/150‑1 (MQ=255)
         cacaCTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGt                                                                                                                                         >  1:428369/1‑150 (MQ=255)
         cacaCTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGt                                                                                                                                         >  1:298033/1‑150 (MQ=255)
          acacTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGtt                                                                                                                                        <  2:1704931/150‑1 (MQ=255)
            acTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTc                                                                                                                                      <  1:1813826/150‑1 (MQ=255)
             cTTACTCGGGTCTCTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGAGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTcc                                                                                                                                     <  2:3002631/150‑1 (MQ=255)
                     ggTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAg                                                                                                                             >  2:4617814/1‑150 (MQ=255)
                      gTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGa                                                                                                                            <  1:1000664/150‑1 (MQ=255)
                         aCTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTg                                                                                                                         >  2:820659/1‑150 (MQ=255)
                            gCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATa                                                                                                                      <  1:2424640/150‑1 (MQ=255)
                             cGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATaa                                                                                                                     <  1:4196522/150‑1 (MQ=255)
                              ggATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAAt                                                                                                                    >  1:1351473/1‑150 (MQ=255)
                                aTGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGt                                                                                                                  <  1:1142225/150‑1 (MQ=255)
                                aTGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGt                                                                                                                  <  2:1499923/150‑1 (MQ=255)
                                 tGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGTATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTc                                                                                                                 >  2:4247891/1‑150 (MQ=255)
                                 tGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTc                                                                                                                 <  1:4049921/150‑1 (MQ=255)
                                  ggCGTTTCGCCTTTCGCTTCTCATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCg                                                                                                                <  2:2545689/150‑1 (MQ=255)
                                   gCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCgg                                                                                                               <  2:2324939/150‑1 (MQ=255)
                                    cGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgt                                                                                                              <  1:959985/150‑1 (MQ=255)
                                    cGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgt                                                                                                              >  1:2660761/1‑150 (MQ=255)
                                    cGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgt                                                                                                              <  1:3312273/150‑1 (MQ=255)
                                     gTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgtg                                                                                                             <  1:507110/150‑1 (MQ=255)
                                     gTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgtg                                                                                                             >  2:1898104/1‑150 (MQ=255)
                                      tttCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgtgt                                                                                                            >  1:1018282/1‑150 (MQ=255)
                                        tCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTg                                                                                                          <  1:209994/150‑1 (MQ=255)
                                        tCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTg                                                                                                          <  2:432199/150‑1 (MQ=255)
                                        tCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTg                                                                                                          <  2:140752/150‑1 (MQ=255)
                                           ccTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGt                                                                                                       >  1:1167776/1‑150 (MQ=255)
                                            cTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGtt                                                                                                      >  2:2732566/1‑150 (MQ=255)
                                               tCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCa                                                                                                   >  1:3695965/1‑150 (MQ=255)
                                               tCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCa                                                                                                   <  2:63871/150‑1 (MQ=255)
                                                 gCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAtc                                                                                                 >  2:1354237/1‑150 (MQ=255)
                                                  cTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAtct                                                                                                >  2:156609/1‑150 (MQ=255)
                                                   ttCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAtctc                                                                                               <  1:2382776/150‑1 (MQ=255)
                                                   ttCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAtctc                                                                                               <  1:4374505/150‑1 (MQ=255)
                                                      tAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCAcc                                                                                            <  1:2028927/150‑1 (MQ=255)
                                                       aaTGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCt                                                                                           <  1:3383212/150‑1 (MQ=255)
                                                       aaTGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCt                                                                                           <  2:3976201/150‑1 (MQ=255)
                                                        aTGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTc                                                                                          <  1:39003/150‑1 (MQ=255)
                                                        aTGCCAGTTGACGCAAGGCCTGGCAGATATAAACGAAGCAGGCGGCATCCGGCTCTAGCGACTGTATATAAGCGTCGAGATGATCTTGCATTATGTACTTCGTATCCAAAAACAGATTGATAAAGTCCGATTTGAGATGAAACTCACCTc                                                                                          >  1:2612972/1‑150 (MQ=255)
                                                            cAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCt                                                                                      >  2:2937126/1‑150 (MQ=255)
                                                             aGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTg                                                                                     <  2:666892/150‑1 (MQ=255)
                                                             aGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTg                                                                                     >  2:3033199/1‑150 (MQ=255)
                                                                tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCt                                                                                  >  1:689344/1‑150 (MQ=255)
                                                                tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCt                                                                                  <  2:3280547/150‑1 (MQ=255)
                                                                tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCt                                                                                  >  2:4493985/1‑150 (MQ=255)
                                                                 gACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCtt                                                                                 >  1:431795/1‑150 (MQ=255)
                                                                   cGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCa                                                                               >  1:2194595/1‑150 (MQ=255)
                                                                      aaGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCg                                                                            >  2:768590/1‑150 (MQ=255)
                                                                      aaGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCg                                                                            >  1:2158012/1‑150 (MQ=255)
                                                                      aaGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCg                                                                            >  2:2215898/1‑150 (MQ=255)
                                                                        ggCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAg                                                                          >  1:2288990/1‑150 (MQ=255)
                                                                          ccTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCa                                                                        <  2:4204123/150‑1 (MQ=255)
                                                                           cTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAg                                                                       <  2:89680/150‑1 (MQ=255)
                                                                           cTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAg                                                                       <  1:3261604/150‑1 (MQ=255)
                                                                            tGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAgg                                                                      >  1:3608161/1‑150 (MQ=255)
                                                                                aGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGtttt                                                                  <  1:4080625/150‑1 (MQ=255)
                                                                                aGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGtttt                                                                  >  1:4274631/1‑150 (MQ=255)
                                                                                aGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGtttt                                                                  <  1:4080605/150‑1 (MQ=255)
                                                                                     taATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATc                                                             <  1:3186282/150‑1 (MQ=255)
                                                                                     taATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATc                                                             <  1:672831/150‑1 (MQ=255)
                                                                                      aaTCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCa                                                            >  2:3855533/1‑150 (MQ=255)
                                                                                      aaTCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCa                                                            >  1:4449990/1‑150 (MQ=255)
                                                                                         cGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGAt                                                         >  1:2170637/1‑150 (MQ=255)
                                                                                          gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATg                                                        >  2:3709781/1‑150 (MQ=255)
                                                                                          gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATg                                                        <  2:2820651/150‑1 (MQ=255)
                                                                                           aaGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGc                                                       <  2:2219823/150‑1 (MQ=255)
                                                                                            aGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCg                                                      >  1:3968502/1‑150 (MQ=255)
                                                                                             gCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGt                                                     <  1:625224/150‑1 (MQ=255)
                                                                                               tGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTgg                                                   >  2:3799781/1‑150 (MQ=255)
                                                                                                 gcggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGtt                                                 >  2:1251537/1‑150 (MQ=255)
                                                                                                      aTCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTg                                            >  1:475964/1‑150 (MQ=255)
                                                                                                           gCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCaaaa                                       >  2:2406423/1‑150 (MQ=255)
                                                                                                            ctctTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAAc                                      <  2:3453434/150‑1 (MQ=255)
                                                                                                                tGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTg                                  >  2:3785156/1‑150 (MQ=255)
                                                                                                                  cGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGaa                                <  2:1085470/150‑1 (MQ=255)
                                                                                                                  cGACTGTTTATAAGCGTCGAGCTGTTCTTGCACTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGaa                                <  2:3497831/150‑1 (MQ=255)
                                                                                                                   gACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAg                               >  1:2925215/1‑150 (MQ=255)
                                                                                                                     cTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGcc                             <  2:1198969/150‑1 (MQ=255)
                                                                                                                         ttATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCaaaa                         <  2:2054678/150‑1 (MQ=255)
                                                                                                                            taAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGtt                      <  1:3190034/150‑1 (MQ=255)
                                                                                                                             aaGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTc                     >  1:3219764/1‑150 (MQ=255)
                                                                                                                               gCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCt                   <  2:1873361/150‑1 (MQ=255)
                                                                                                                               gCGTCGAGCTGTTCTTGCATTATGTCCTTCGGTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCt                   <  2:1873230/150‑1 (MQ=255)
                                                                                                                                   cGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGccc               >  2:3078037/1‑150 (MQ=255)
                                                                                                                                   cGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGccc               >  1:2201895/1‑150 (MQ=255)
                                                                                                                                     aGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCcct             >  2:944402/1‑150 (MQ=255)
                                                                                                                                         gTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCtt         >  2:1013840/1‑150 (MQ=255)
                                                                                                                                           tattGCATTATGTCCTTAGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTg       <  1:3520582/148‑1 (MQ=255)
                                                                                                                                           tCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTg       <  2:4641187/150‑1 (MQ=255)
                                                                                                                                           tCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTg       <  1:1255492/150‑1 (MQ=255)
                                                                                                                                              tGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATc    <  2:2613122/150‑1 (MQ=255)
                                                                                                                                              tGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATc    >  1:4345262/1‑150 (MQ=255)
                                                                                                                                                cATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGa  >  2:316368/1‑150 (MQ=255)
                                                                                                                                                cATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCAACAGATTCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGa  >  2:710671/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGA  >  CP009273/1969144‑1969437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  CP009273/1969143‑1969438
                                                                                                                                                    |                                                                                                                                                   
TTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTA                                                                                                                                                    <  SRR19973072.3829289/150‑1 (MQ=60)
 TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAT                                                                                                                                                   <  SRR19973072.3953856/150‑1 (MQ=60)
  TTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATG                                                                                                                                                  <  SRR19973072.2843035/150‑1 (MQ=60)
  TTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATG                                                                                                                                                  <  SRR19973072.2842984/150‑1 (MQ=60)
  TTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATG                                                                                                                                                  <  SRR19973072.312402/150‑1 (MQ=60)
  TTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATG                                                                                                                                                  <  SRR19973072.1256847/150‑1 (MQ=60)
  TTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATG                                                                                                                                                  <  SRR19973072.1256940/150‑1 (MQ=60)
   TGGCCACCACACTAAATCGCGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTACTGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTNTATAAGCGTCGAGCTGTTCTTGCATTATGT                                                                                                                                                 <  SRR19973072.4293720/150‑1 (MQ=60)
     GCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCC                                                                                                                                               <  SRR19973072.3205776/150‑1 (MQ=60)
         CCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCG                                                                                                                                           >  SRR19973072.1150741/1‑150 (MQ=60)
          CACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGT                                                                                                                                          >  SRR19973072.298730/1‑150 (MQ=60)
          CACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGT                                                                                                                                          >  SRR19973072.429354/1‑150 (MQ=60)
             ACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTC                                                                                                                                       <  SRR19973072.1817777/150‑1 (MQ=60)
              CTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCC                                                                                                                                      <  SRR19973072.627890/150‑1 (MQ=60)
                  ATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAA                                                                                                                                  >  SRR19973072.4612263/1‑150 (MQ=60)
                    CGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAAC                                                                                                                                >  SRR19973072.2560915/1‑150 (MQ=60)
                    CGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAAC                                                                                                                                >  SRR19973072.4368761/1‑150 (MQ=60)
                       GTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGA                                                                                                                             <  SRR19973072.1002854/150‑1 (MQ=60)
                       GTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGA                                                                                                                             >  SRR19973072.1088273/1‑150 (MQ=60)
                          ACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTG                                                                                                                          >  SRR19973072.2167320/1‑150 (MQ=60)
                           CTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGA                                                                                                                         <  SRR19973072.3501948/150‑1 (MQ=60)
                             GCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATA                                                                                                                       <  SRR19973072.2429896/150‑1 (MQ=60)
                             GCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATA                                                                                                                       <  SRR19973072.1296111/150‑1 (MQ=60)
                             GCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATA                                                                                                                       <  SRR19973072.4060448/150‑1 (MQ=60)
                              CGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAA                                                                                                                      <  SRR19973072.4205302/150‑1 (MQ=60)
                               GGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAAT                                                                                                                     >  SRR19973072.1354433/1‑150 (MQ=60)
                                 ATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGT                                                                                                                   <  SRR19973072.1144612/150‑1 (MQ=60)
                                  TGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTC                                                                                                                  <  SRR19973072.4339026/150‑1 (MQ=60)
                                  TGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTC                                                                                                                  <  SRR19973072.4058507/150‑1 (MQ=60)
                                  TGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTC                                                                                                                  <  SRR19973072.4058422/150‑1 (MQ=60)
                                   GGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCG                                                                                                                 <  SRR19973072.679876/150‑1 (MQ=60)
                                     CGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGT                                                                                                               >  SRR19973072.2666457/1‑150 (MQ=60)
                                     CGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGT                                                                                                               <  SRR19973072.962089/150‑1 (MQ=60)
                                     CGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGT                                                                                                               <  SRR19973072.3319309/150‑1 (MQ=60)
                                      GTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTG                                                                                                              <  SRR19973072.508319/150‑1 (MQ=60)
                                       TTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGT                                                                                                             >  SRR19973072.1020493/1‑150 (MQ=60)
                                        TTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTT                                                                                                            <  SRR19973072.223384/150‑1 (MQ=60)
                                         TCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTTTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTG                                                                                                           <  SRR19973072.210721/150‑1 (MQ=60)
                                         TCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTG                                                                                                           <  SRR19973072.210521/150‑1 (MQ=60)
                                           GCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAG                                                                                                         <  SRR19973072.974420/150‑1 (MQ=60)
                                            CCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGT                                                                                                        >  SRR19973072.1170222/1‑150 (MQ=60)
                                            CCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGT                                                                                                        >  SRR19973072.4142780/1‑150 (MQ=60)
                                                TCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCA                                                                                                    >  SRR19973072.3703753/1‑150 (MQ=60)
                                                TCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCA                                                                                                    <  SRR19973072.1004115/150‑1 (MQ=60)
                                                   CTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCT                                                                                                 <  SRR19973072.714151/150‑1 (MQ=60)
                                                    TTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTC                                                                                                <  SRR19973072.4384001/150‑1 (MQ=60)
                                                    TTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTC                                                                                                <  SRR19973072.2387920/150‑1 (MQ=60)
                                                    TTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTC                                                                                                <  SRR19973072.4383697/150‑1 (MQ=60)
                                                       TAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACC                                                                                             <  SRR19973072.2033320/150‑1 (MQ=60)
                                                        AATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCT                                                                                            >  SRR19973072.1823306/1‑150 (MQ=60)
                                                        AATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCT                                                                                            <  SRR19973072.3390363/150‑1 (MQ=60)
                                                        AATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCT                                                                                            <  SRR19973072.3390235/150‑1 (MQ=60)
                                                         ATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTC                                                                                           <  SRR19973072.3822595/150‑1 (MQ=60)
                                                         ATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTC                                                                                           >  SRR19973072.2618752/1‑150 (MQ=60)
                                                         ATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTC                                                                                           <  SRR19973072.39112/150‑1 (MQ=60)
                                                         ATGCCAGTTGACGCAAGGCCTGGCAGATATAAACGAAGCAGGCGGCATCCGGCTCTAGCGACTGTATATAAGCGTCGAGATGATCTTGCATTATGTACTTCGTATCCAAAAACAGATTGATAAagtccgatttgagatgaaactcacctc                                                                                           >  SRR19973072.2618601/1‑123 (MQ=60)
                                                          TGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCG                                                                                          >  SRR19973072.3317649/1‑150 (MQ=60)
                                                           GCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGT                                                                                         >  SRR19973072.2331804/1‑150 (MQ=60)
                                                                 TGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCT                                                                                   >  SRR19973072.690867/1‑150 (MQ=60)
                                                                 TGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCT                                                                                   >  SRR19973072.690991/1‑150 (MQ=60)
                                                                  GACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT                                                                                  >  SRR19973072.432786/1‑150 (MQ=60)
                                                                  GACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTAGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTT                                                                                  >  SRR19973072.432777/1‑150 (MQ=60)
                                                                    CGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCA                                                                                >  SRR19973072.2199372/1‑150 (MQ=60)
                                                                     GCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCAT                                                                               <  SRR19973072.489868/150‑1 (MQ=60)
                                                                       AAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCG                                                                             >  SRR19973072.2162714/1‑150 (MQ=60)
                                                                       AAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCG                                                                             >  SRR19973072.3065881/1‑150 (MQ=60)
                                                                         GGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAG                                                                           >  SRR19973072.2293895/1‑150 (MQ=60)
                                                                            CTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAG                                                                        <  SRR19973072.3268525/150‑1 (MQ=60)
                                                                            CTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAG                                                                        <  SRR19973072.3268485/150‑1 (MQ=60)
                                                                            CTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAG                                                                        <  SRR19973072.2204044/150‑1 (MQ=60)
                                                                             TGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGG                                                                       >  SRR19973072.3615764/1‑150 (MQ=60)
                                                                                 AGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTT                                                                   <  SRR19973072.4089170/150‑1 (MQ=60)
                                                                                 AGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTT                                                                   <  SRR19973072.4089184/150‑1 (MQ=60)
                                                                                 AGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTT                                                                   <  SRR19973072.4089190/150‑1 (MQ=60)
                                                                                 AGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTT                                                                   >  SRR19973072.4283556/1‑150 (MQ=60)
                                                                                 AGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTT                                                                   >  SRR19973072.4283528/1‑150 (MQ=60)
                                                                                     ATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCAT                                                               >  SRR19973072.4228661/1‑150 (MQ=60)
                                                                                      TAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATC                                                              <  SRR19973072.3193041/150‑1 (MQ=60)
                                                                                      TAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATC                                                              <  SRR19973072.3193003/150‑1 (MQ=60)
                                                                                      TAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATC                                                              <  SRR19973072.674331/150‑1 (MQ=60)
                                                                                       AATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCA                                                             >  SRR19973072.4459372/1‑150 (MQ=60)
                                                                                        ATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAG                                                            >  SRR19973072.3968032/1‑150 (MQ=60)
                                                                                        ATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAG                                                            >  SRR19973072.1473506/1‑150 (MQ=60)
                                                                                          CGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGAT                                                          >  SRR19973072.2175368/1‑150 (MQ=60)
                                                                                             AGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCG                                                       >  SRR19973072.3976805/1‑150 (MQ=60)
                                                                                              GCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGT                                                      <  SRR19973072.626655/150‑1 (MQ=60)
                                                                                                  GCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTT                                                  <  SRR19973072.3227662/150‑1 (MQ=60)
                                                                                                  GCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTT                                                  <  SRR19973072.2756859/150‑1 (MQ=60)
                                                                                                   TGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTT                                                 <  SRR19973072.3051083/150‑1 (MQ=60)
                                                                                                       ATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTG                                             >  SRR19973072.477078/1‑150 (MQ=60)
                                                                                                        TCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGC                                            >  SRR19973072.3987265/1‑150 (MQ=60)
                                                                                                               CTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGC                                     >  SRR19973072.53033/1‑150 (MQ=60)
                                                                                                                   CGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAA                                 <  SRR19973072.4282503/150‑1 (MQ=60)
                                                                                                                    GACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAG                                >  SRR19973072.2931472/1‑150 (MQ=60)
                                                                                                                      CTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCC                              >  SRR19973072.3186834/1‑150 (MQ=60)
                                                                                                                       TGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCA                             >  SRR19973072.3613778/1‑150 (MQ=60)
                                                                                                                             TAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTT                       <  SRR19973072.2343059/150‑1 (MQ=60)
                                                                                                                             TAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTT                       <  SRR19973072.3196765/150‑1 (MQ=60)
                                                                                                                              AAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTC                      >  SRR19973072.2954300/1‑150 (MQ=60)
                                                                                                                              AAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTC                      >  SRR19973072.3226578/1‑150 (MQ=60)
                                                                                                                                GCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCT                    <  SRR19973072.3909535/150‑1 (MQ=60)
                                                                                                                                GCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCT                    <  SRR19973072.3909496/150‑1 (MQ=60)
                                                                                                                                    CGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCC                >  SRR19973072.2206685/1‑150 (MQ=60)
                                                                                                                                    CGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCC                >  SRR19973072.3690931/1‑150 (MQ=60)
                                                                                                                                    CGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGAGGTTTCCAGCAAAACGCTGAAGCCAAAAGATCCTGCCC                >  SRR19973072.506471/1‑150 (MQ=60)
                                                                                                                                          GTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCTTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAATCCAAAAGTTCCTGCCCCTCCTT          >  SRR19973072.2051052/1‑150 (MQ=60)
                                                                                                                                           TTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTT         <  SRR19973072.2185158/150‑1 (MQ=60)
                                                                                                                                           TTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTT         <  SRR19973072.2185254/150‑1 (MQ=60)
                                                                                                                                            TCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTG        <  SRR19973072.2426426/150‑1 (MQ=60)
                                                                                                                                            TCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTG        <  SRR19973072.1258193/150‑1 (MQ=60)
                                                                                                                                            TATTGCATTATGTCCTTAGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTG        <  SRR19973072.3527969/150‑1 (MQ=60)
                                                                                                                                             CTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGA       >  SRR19973072.2704965/1‑150 (MQ=60)
                                                                                                                                               TGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATC     >  SRR19973072.4354389/1‑150 (MQ=60)
                                                                                                                                               TGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATC     >  SRR19973072.4354364/1‑150 (MQ=60)
                                                                                                                                                 CATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGA   >  SRR19973072.1956817/1‑150 (MQ=60)
                                                                                                                                                 CATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGA   <  SRR19973072.3472219/150‑1 (MQ=60)
                                                                                                                                                  ATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  <  SRR19973072.4198717/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
TTTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  CP009273/1969143‑1969438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑