Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,969,291 G→T 100% I97I (ATC→ATA)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,969,2910GT100.0% 589.5 / NA 180I97I (ATC→ATA) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (79/101);  total (79/101)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

TGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGA  >  CP009273/1969146‑1969433
                                                                                                                                                 |                                                                                                                                              
tGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattat                                                                                                                                              <  1:3769071/148‑1 (MQ=255)
tGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattat                                                                                                                                              <  2:3534447/148‑1 (MQ=255)
tGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattat                                                                                                                                              <  2:1744994/148‑1 (MQ=255)
 ggCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCattatg                                                                                                                                             <  2:138717/148‑1 (MQ=255)
    aaCCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTcc                                                                                                                                          <  2:2509257/148‑1 (MQ=255)
    aaCCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTcc                                                                                                                                          <  1:2409360/148‑1 (MQ=255)
     aCCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCt                                                                                                                                         <  1:3581046/148‑1 (MQ=255)
     aCCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCt                                                                                                                                         <  1:2189168/148‑1 (MQ=255)
         cacTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGt                                                                                                                                     <  2:712701/148‑1 (MQ=255)
         cacTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGt                                                                                                                                     <  2:1052049/148‑1 (MQ=255)
          acTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGtt                                                                                                                                    <  2:3506081/148‑1 (MQ=255)
           cTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGttt                                                                                                                                   <  1:3257151/148‑1 (MQ=255)
            ttAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTc                                                                                                                                  <  2:2663230/148‑1 (MQ=255)
            ttAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTc                                                                                                                                  >  1:2340236/1‑148 (MQ=255)
            ttAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTc                                                                                                                                  >  2:2360921/1‑148 (MQ=255)
               aTCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaa                                                                                                                               >  2:2092627/1‑148 (MQ=255)
               aTCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaa                                                                                                                               >  1:508451/1‑148 (MQ=255)
               aTCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaa                                                                                                                               >  2:2649003/1‑148 (MQ=255)
                tCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaaa                                                                                                                              >  2:254712/1‑148 (MQ=255)
                 cGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaaaa                                                                                                                             <  1:2369456/148‑1 (MQ=255)
                 cGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaaaa                                                                                                                             <  2:221988/148‑1 (MQ=255)
                 cGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCaaaa                                                                                                                             <  2:2278574/148‑1 (MQ=255)
                     tCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAg                                                                                                                         <  2:3313543/148‑1 (MQ=255)
                      cACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGa                                                                                                                        >  1:3633155/1‑148 (MQ=255)
                         tGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTg                                                                                                                     <  1:3434947/148‑1 (MQ=255)
                         tGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGAATGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTg                                                                                                                     <  1:2747854/148‑1 (MQ=255)
                            ggATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATa                                                                                                                  <  2:1294857/148‑1 (MQ=255)
                            ggATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATa                                                                                                                  <  2:1086251/148‑1 (MQ=255)
                             gATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATaa                                                                                                                 >  1:1097466/1‑148 (MQ=255)
                              aTGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAAt                                                                                                                >  1:810270/1‑148 (MQ=255)
                                  cGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCg                                                                                                            <  2:3124721/148‑1 (MQ=255)
                                    tttCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGgt                                                                                                          >  1:259095/1‑148 (MQ=255)
                                     ttCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATCATGTCGgtg                                                                                                         >  2:2060703/1‑148 (MQ=255)
                                        gCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTg                                                                                                      >  1:1713575/1‑148 (MQ=255)
                                        gCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTg                                                                                                      <  1:1659936/148‑1 (MQ=255)
                                         ccTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGa                                                                                                     >  1:2462631/1‑148 (MQ=255)
                                         ccTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGACATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGa                                                                                                     >  1:1031044/1‑148 (MQ=255)
                                           tttCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGt                                                                                                   <  1:2449415/148‑1 (MQ=255)
                                            ttCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGtt                                                                                                  >  1:1448752/1‑148 (MQ=255)
                                            ttCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGGGTTGAGtt                                                                                                  >  2:130780/1‑148 (MQ=255)
                                               gCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCa                                                                                               <  1:1664115/148‑1 (MQ=255)
                                                cTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAt                                                                                              <  2:3617013/148‑1 (MQ=255)
                                                  tCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAtct                                                                                            <  1:440254/148‑1 (MQ=255)
                                                      aTGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCAcc                                                                                        >  1:1345955/1‑148 (MQ=255)
                                                         ccAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCg                                                                                     >  1:169586/1‑148 (MQ=255)
                                                              tGACGCAAGGCCTGGCATATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTgg                                                                                <  2:1233834/148‑1 (MQ=255)
                                                              tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTgg                                                                                <  2:3336573/148‑1 (MQ=255)
                                                              tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTgg                                                                                <  2:1895567/148‑1 (MQ=255)
                                                              tGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTgg                                                                                <  2:1899143/148‑1 (MQ=255)
                                                                aCGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCt                                                                              <  2:1102529/148‑1 (MQ=255)
                                                                aCGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCt                                                                              <  2:406862/148‑1 (MQ=255)
                                                                  gCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTc                                                                            <  2:3795389/148‑1 (MQ=255)
                                                                    aaGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCAt                                                                          <  1:2499826/148‑1 (MQ=255)
                                                                     aGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATc                                                                         >  1:1999635/1‑148 (MQ=255)
                                                                      ggCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCg                                                                        <  1:2813747/148‑1 (MQ=255)
                                                                          tGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCa                                                                    <  2:1713575/148‑1 (MQ=255)
                                                                            gCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAgg                                                                  <  1:702136/148‑1 (MQ=255)
                                                                               gATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGttt                                                               >  1:2985424/1‑148 (MQ=255)
                                                                                atatAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGtttt                                                              >  2:2720929/1‑148 (MQ=255)
                                                                                atatAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGtttt                                                              <  1:2276045/148‑1 (MQ=255)
                                                                                  ataATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTcc                                                            <  2:1476524/148‑1 (MQ=255)
                                                                                  ataATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTcc                                                            <  2:1477074/148‑1 (MQ=255)
                                                                                   taATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCa                                                           >  1:233465/1‑148 (MQ=255)
                                                                                       cGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAg                                                       <  2:3623012/148‑1 (MQ=255)
                                                                                        gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGa                                                      <  1:3240471/148‑1 (MQ=255)
                                                                                        gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGa                                                      <  1:3501809/148‑1 (MQ=255)
                                                                                        gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGa                                                      <  1:3501818/148‑1 (MQ=255)
                                                                                        gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGa                                                      <  2:1986654/148‑1 (MQ=255)
                                                                                        gAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGa                                                      <  2:755458/148‑1 (MQ=255)
                                                                                         aaGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGAt                                                     <  1:3072025/148‑1 (MQ=255)
                                                                                           gCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGc                                                   >  2:1243639/1‑148 (MQ=255)
                                                                                              ggcggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTg                                                <  2:675489/148‑1 (MQ=255)
                                                                                               gcggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTgg                                               >  1:1595260/1‑148 (MQ=255)
                                                                                               gcggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTgg                                               <  1:3704522/148‑1 (MQ=255)
                                                                                               gcggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAGGTCCTTCGTTTCAAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAGCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTgg                                               >  1:3494108/1‑148 (MQ=255)
                                                                                                cggcATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGAATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGt                                              <  1:2066540/148‑1 (MQ=255)
                                                                                                   cATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTc                                           >  2:76828/1‑148 (MQ=255)
                                                                                                    aTCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTcc                                          <  2:1542552/148‑1 (MQ=255)
                                                                                                    aTCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTcc                                          <  2:3222001/148‑1 (MQ=255)
                                                                                                              tGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGc                                >  2:3380974/1‑148 (MQ=255)
                                                                                                                cGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTg                              <  1:2649003/148‑1 (MQ=255)
                                                                                                                 gACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGa                             <  2:2905246/148‑1 (MQ=255)
                                                                                                                 gACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGa                             <  1:1324937/148‑1 (MQ=255)
                                                                                                                   cTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAg                           >  2:3098993/1‑148 (MQ=255)
                                                                                                                   cTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAg                           >  2:462482/1‑148 (MQ=255)
                                                                                                                    tGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGc                          >  2:58470/1‑148 (MQ=255)
                                                                                                                       ttATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCaa                       >  1:1834423/1‑148 (MQ=255)
                                                                                                                        tataAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCaaa                      <  2:3031965/148‑1 (MQ=255)
                                                                                                                            aGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGtt                  <  2:2317589/148‑1 (MQ=255)
                                                                                                                              cGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTcc                >  1:3011733/1‑148 (MQ=255)
                                                                                                                              cGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTcc                >  2:2013051/1‑148 (MQ=255)
                                                                                                                                tCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTTCATCTCACATCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTg              >  1:3444097/1‑148 (MQ=255)
                                                                                                                                   aGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGccc           <  1:76828/148‑1 (MQ=255)
                                                                                                                                   aGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGccc           <  1:3775465/148‑1 (MQ=255)
                                                                                                                                     cTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCcct         >  2:3525035/1‑148 (MQ=255)
                                                                                                                                       gTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCcctcc       >  2:3572708/1‑148 (MQ=255)
                                                                                                                                       gTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCcctcc       >  2:1678628/1‑148 (MQ=255)
                                                                                                                                        ttCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCcctcct      >  1:981518/1‑148 (MQ=255)
                                                                                                                                           ttGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTg   <  2:2232226/148‑1 (MQ=255)
                                                                                                                                            tGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCGATCAGATGCGTGGTTTCCTGGAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGa  <  2:1228429/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGA  >  CP009273/1969146‑1969433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  CP009273/1969144‑1969438
                                                                                                                                                   |                                                                                                                                                   
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAT                                                                                                                                                   <  SRR19973069.834694/150‑1 (MQ=60)
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAT                                                                                                                                                   <  SRR19973069.1883811/150‑1 (MQ=60)
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAT                                                                                                                                                   <  SRR19973069.1882626/150‑1 (MQ=60)
  TGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGT                                                                                                                                                 <  SRR19973069.3794406/150‑1 (MQ=60)
  TGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGT                                                                                                                                                 <  SRR19973069.3793559/150‑1 (MQ=60)
      AACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTT                                                                                                                                             <  SRR19973069.2425653/150‑1 (MQ=60)
       ACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTC                                                                                                                                            <  SRR19973069.2204072/150‑1 (MQ=60)
       ACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTC                                                                                                                                            <  SRR19973069.167908/150‑1 (MQ=60)
       ACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTC                                                                                                                                            <  SRR19973069.3604398/150‑1 (MQ=60)
        CCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCG                                                                                                                                           >  SRR19973069.3646688/1‑150 (MQ=60)
        CCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCG                                                                                                                                           <  SRR19973069.3535391/150‑1 (MQ=60)
         CACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGT                                                                                                                                          >  SRR19973069.1361797/1‑150 (MQ=60)
            ACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTC                                                                                                                                       >  SRR19973069.2356156/1‑150 (MQ=60)
             CTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCC                                                                                                                                      <  SRR19973069.3278484/150‑1 (MQ=60)
             CTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCC                                                                                                                                      <  SRR19973069.3277620/150‑1 (MQ=60)
               TAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAA                                                                                                                                    >  SRR19973069.512077/1‑150 (MQ=60)
                   CGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAAC                                                                                                                                <  SRR19973069.2385509/150‑1 (MQ=60)
                   CGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAAC                                                                                                                                <  SRR19973069.2385536/150‑1 (MQ=60)
                      GTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGA                                                                                                                             >  SRR19973069.3656908/1‑150 (MQ=60)
                      GTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGA                                                                                                                             >  SRR19973069.1911696/1‑150 (MQ=60)
                      GTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGA                                                                                                                             >  SRR19973069.3656932/1‑150 (MQ=60)
                          CTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGA                                                                                                                         <  SRR19973069.3231534/150‑1 (MQ=60)
                           TGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGAT                                                                                                                        <  SRR19973069.3457267/150‑1 (MQ=60)
                           TGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGAT                                                                                                                        <  SRR19973069.2766968/150‑1 (MQ=60)
                           TGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGAATGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGAT                                                                                                                        <  SRR19973069.2766210/150‑1 (MQ=60)
                             CGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAA                                                                                                                      >  SRR19973069.1105133/1‑150 (MQ=60)
                              GGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAAT                                                                                                                     >  SRR19973069.816006/1‑150 (MQ=60)
                                ATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCTAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGT                                                                                                                   <  SRR19973069.2590064/150‑1 (MQ=60)
                                    CGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGT                                                                                                               >  SRR19973069.3505689/1‑150 (MQ=60)
                                    CGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGT                                                                                                               >  SRR19973069.260968/1‑150 (MQ=60)
                                        TCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTG                                                                                                           >  SRR19973069.1725581/1‑150 (MQ=60)
                                         CGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGA                                                                                                          >  SRR19973069.1771227/1‑150 (MQ=60)
                                         CGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGA                                                                                                          >  SRR19973069.2479164/1‑150 (MQ=60)
                                         CGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGACATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGA                                                                                                          >  SRR19973069.1038183/1‑150 (MQ=60)
                                          GCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAG                                                                                                         <  SRR19973069.1671431/150‑1 (MQ=60)
                                          GCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGATGCGAGCTGTTATGTAATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAG                                                                                                         <  SRR19973069.3770526/150‑1 (MQ=60)
                                            CTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTT                                                                                                       >  SRR19973069.1458864/1‑150 (MQ=60)
                                            CTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTT                                                                                                       >  SRR19973069.1501688/1‑150 (MQ=60)
                                             TTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTG                                                                                                      <  SRR19973069.2465895/150‑1 (MQ=60)
                                             TTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTG                                                                                                      <  SRR19973069.2465912/150‑1 (MQ=60)
                                                CGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAT                                                                                                   <  SRR19973069.3403446/150‑1 (MQ=60)
                                                CGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAT                                                                                                   <  SRR19973069.2314475/150‑1 (MQ=60)
                                                 GCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATC                                                                                                  <  SRR19973069.1675648/150‑1 (MQ=60)
                                                    TCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCA                                                                                               <  SRR19973069.443480/150‑1 (MQ=60)
                                                      TAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACC                                                                                             >  SRR19973069.1355274/1‑150 (MQ=60)
                                                      TAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACC                                                                                             >  SRR19973069.1355245/1‑150 (MQ=60)
                                                         TGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCG                                                                                          >  SRR19973069.171295/1‑150 (MQ=60)
                                                         TGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCG                                                                                          >  SRR19973069.171035/1‑150 (MQ=60)
                                                                   CGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCA                                                                                >  SRR19973069.472425/1‑150 (MQ=60)
                                                                     CAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATC                                                                              >  SRR19973069.737968/1‑150 (MQ=60)
                                                                     CAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATC                                                                              >  SRR19973069.2013568/1‑150 (MQ=60)
                                                                      AAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCG                                                                             <  SRR19973069.2516523/150‑1 (MQ=60)
                                                                       AGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGA                                                                            >  SRR19973069.2351021/1‑150 (MQ=60)
                                                                        GGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAG                                                                           <  SRR19973069.2832385/150‑1 (MQ=60)
                                                                        GGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAG                                                                           <  SRR19973069.126949/150‑1 (MQ=60)
                                                                              GCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTT                                                                     <  SRR19973069.707075/150‑1 (MQ=60)
                                                                               CAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTT                                                                    >  SRR19973069.3004958/1‑150 (MQ=60)
                                                                               CAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTT                                                                    >  SRR19973069.3005251/1‑150 (MQ=60)
                                                                                  ATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCC                                                                 <  SRR19973069.2291490/150‑1 (MQ=60)
                                                                                   TATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCA                                                                >  SRR19973069.235238/1‑150 (MQ=60)
                                                                                          GAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATG                                                         <  SRR19973069.3524365/150‑1 (MQ=60)
                                                                                          GAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATG                                                         <  SRR19973069.3524362/150‑1 (MQ=60)
                                                                                          GAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATG                                                         <  SRR19973069.3524356/150‑1 (MQ=60)
                                                                                          GAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATG                                                         <  SRR19973069.3261683/150‑1 (MQ=60)
                                                                                           AAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGC                                                        <  SRR19973069.3092314/150‑1 (MQ=60)
                                                                                               TGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGG                                                    >  SRR19973069.1606048/1‑150 (MQ=60)
                                                                                               TGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGG                                                    >  SRR19973069.1606030/1‑150 (MQ=60)
                                                                                               TGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTAGGTCCTTCGTTTCAAAAAACAGATTGATAATGTCGGTGTTGAGTTGCAGCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGG                                                    >  SRR19973069.3516626/1‑150 (MQ=60)
                                                                                                 GCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTT                                                  <  SRR19973069.3728729/150‑1 (MQ=60)
                                                                                                  CGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGAATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTT                                                 <  SRR19973069.2080867/150‑1 (MQ=60)
                                                                                                   GGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTC                                                >  SRR19973069.2121210/1‑150 (MQ=60)
                                                                                                          GGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAA                                         >  SRR19973069.2650188/1‑150 (MQ=60)
                                                                                                                TGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTG                                   >  SRR19973069.3041894/1‑150 (MQ=60)
                                                                                                                  CGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAA                                 <  SRR19973069.2666771/150‑1 (MQ=60)
                                                                                                                  CGACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAA                                 <  SRR19973069.2666747/150‑1 (MQ=60)
                                                                                                                   GACTGTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAG                                <  SRR19973069.1334045/150‑1 (MQ=60)
                                                                                                                       GTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAA                            >  SRR19973069.1847265/1‑150 (MQ=60)
                                                                                                                       GTTTATAAGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAA                            <  SRR19973069.791904/150‑1 (MQ=60)
                                                                                                                              AGCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCC                     >  SRR19973069.3031718/1‑150 (MQ=60)
                                                                                                                               GCGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCT                    <  SRR19973069.684746/150‑1 (MQ=60)
                                                                                                                                CGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTTCATCTCACATCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTG                   >  SRR19973069.3466447/1‑150 (MQ=60)
                                                                                                                                CGTCGAGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTG                   >  SRR19973069.3466428/1‑150 (MQ=60)
                                                                                                                                     AGCTGTTCTTGCATTATTTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTAGTCTGTCTTCATCGAGCAGGATTTCCATCCGATGCGTGGTTTCCTGCAAAACGCTGTAGCCAAAAGTTCCTGCCCCT              <  SRR19973069.2563942/150‑1 (MQ=60)
                                                                                                                                     AGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCT              <  SRR19973069.2052949/150‑1 (MQ=60)
                                                                                                                                     AGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCT              <  SRR19973069.3799989/150‑1 (MQ=60)
                                                                                                                                     AGCTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCT              <  SRR19973069.77672/150‑1 (MQ=60)
                                                                                                                                       CTGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTACATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCC            >  SRR19973069.2396720/1‑150 (MQ=60)
                                                                                                                                        TGTTCTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCT           >  SRR19973069.988144/1‑150 (MQ=60)
                                                                                                                                            CTTGCATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGA       >  SRR19973069.888313/1‑150 (MQ=60)
                                                                                                                                                 ATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  SRR19973069.1185652/1‑150 (MQ=60)
                                                                                                                                                 ATTATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  SRR19973069.1071024/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
TTTGGCAACCACACTTAATCGGGTCACTGCGGATGGCGTTTCGCCTTTCGCTTCTAATGCCAGTTGACGCAAGGCCTGGCAGATATAATCGAAGCTGGCGGCATCCGGCTCTTGCGACTGTTTATAAGCGTCGAGCTGTTCTTGCATGATGTCCTTCGTTTCCAAAAACAGATTGATAATGTCGGTGTTGAGTTGCATCTCACCTCGTCTGGCTTCATCGAGCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAG  >  CP009273/1969144‑1969438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑