Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,225,190 C→T 100% G231G (GGC→GGT)  yohK → LrgB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,225,1900CT97.3% 898.3 / 9.6 260G231G (GGC→GGT) yohKLrgB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (5/2);  new base T (125/128);  total (130/130)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 260 total aligned reads displayed.

CTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225045‑2225336
                                                                                                                                                 |                                                                                                                                                  
cTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaa                                                                                                                                                >  1:3028438/1‑150 (MQ=255)
 tGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaaa                                                                                                                                               <  2:4582075/150‑1 (MQ=255)
 tGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaaa                                                                                                                                               <  2:2156426/150‑1 (MQ=255)
      cGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGc                                                                                                                                          <  1:4429846/150‑1 (MQ=255)
       gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCg                                                                                                                                         >  1:759791/1‑150 (MQ=255)
        cACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGa                                                                                                                                        >  2:2048397/1‑150 (MQ=255)
         aCGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGAt                                                                                                                                       >  2:1259567/1‑150 (MQ=255)
         aCGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGAt                                                                                                                                       >  2:1595171/1‑150 (MQ=255)
          cGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                      <  2:2652342/150‑1 (MQ=255)
           gCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCCCTGGTGATATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCAGATTATTCTGGCAGTAATGGGTTAAAATTAGCGATGc                                                                                                                                     >  2:2606191/1‑150 (MQ=255)
            cccTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCg                                                                                                                                    >  2:1332878/1‑150 (MQ=255)
            cccTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCg                                                                                                                                    >  1:3800563/1‑150 (MQ=255)
              cTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTc                                                                                                                                  <  1:3634923/150‑1 (MQ=255)
                      ggCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttt                                                                                                                          >  1:2767540/1‑150 (MQ=255)
                      ggCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttt                                                                                                                          <  1:2496115/150‑1 (MQ=255)
                          cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      >  2:2515909/1‑150 (MQ=255)
                          cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      >  2:69578/1‑150 (MQ=255)
                          cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      >  2:405625/1‑150 (MQ=255)
                          cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      >  2:2174112/1‑150 (MQ=255)
                          cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGAGTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      >  2:69628/1‑150 (MQ=255)
                           gcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                     <  1:638833/150‑1 (MQ=255)
                               cgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtat                                                                                                                 <  1:4587612/150‑1 (MQ=255)
                                 ccGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtatgt                                                                                                               >  1:3136167/1‑150 (MQ=255)
                                  cGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCGGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGtt                                                                                                              <  2:60437/150‑1 (MQ=255)
                                   gAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGttt                                                                                                             >  1:2709205/1‑150 (MQ=255)
                                      cTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAc                                                                                                          >  1:1738944/1‑150 (MQ=255)
                                       tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcg                                                                                                         >  1:1907474/1‑150 (MQ=255)
                                           ttATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGtt                                                                                                     <  2:2684287/150‑1 (MQ=255)
                                              tCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCa                                                                                                  <  2:1817008/150‑1 (MQ=255)
                                                  gAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaat                                                                                              >  2:338093/1‑150 (MQ=255)
                                                   aaGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatt                                                                                             <  1:4085149/150‑1 (MQ=255)
                                                       tGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATg                                                                                         <  1:3255547/150‑1 (MQ=255)
                                                         cATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgag                                                                                       <  2:2913295/150‑1 (MQ=255)
                                                             tAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTc                                                                                   >  1:2253119/1‑150 (MQ=255)
                                                                  cGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATc                                                                              <  2:1968825/150‑1 (MQ=255)
                                                                      aGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACat                                                                          >  1:2348695/1‑150 (MQ=255)
                                                                         gcTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACAtata                                                                       >  2:3729038/1‑150 (MQ=255)
                                                                         gcTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACAtata                                                                       <  2:2026234/150‑1 (MQ=255)
                                                                          cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                      >  2:1893993/1‑150 (MQ=255)
                                                                          cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                      <  1:3508182/150‑1 (MQ=255)
                                                                           tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                     <  2:39817/150‑1 (MQ=255)
                                                                            ggTGTTATGCGGGATAATTACTTCGATGAAAGCACCGTTCCTTTTCCAGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATTCGAAGCTCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAg                                                                    >  2:1165170/1‑150 (MQ=255)
                                                                             gtgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGc                                                                   <  1:86019/150‑1 (MQ=255)
                                                                              tgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGcc                                                                  <  1:2914857/150‑1 (MQ=255)
                                                                               gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                 >  1:489054/1‑150 (MQ=255)
                                                                               gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGAGTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                 >  2:390022/1‑150 (MQ=255)
                                                                                ttATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCcac                                                                <  1:3357506/150‑1 (MQ=255)
                                                                                  aTGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              >  2:1037878/1‑150 (MQ=255)
                                                                                  aTGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              <  1:1694671/150‑1 (MQ=255)
                                                                                   tGCGGGATAATTACTTCGCTGATCGCACAGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                             <  1:3910677/150‑1 (MQ=255)
                                                                                      gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACggg                                                          <  2:109642/150‑1 (MQ=255)
                                                                                       ggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGt                                                         <  2:4336300/150‑1 (MQ=255)
                                                                                         aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  1:4332583/150‑1 (MQ=255)
                                                                                         aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  2:872987/150‑1 (MQ=255)
                                                                                           aaTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGt                                                     >  2:4260891/1‑150 (MQ=255)
                                                                                            aTTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTa                                                    >  2:3490554/1‑150 (MQ=255)
                                                                                             ttACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaa                                                   <  2:2438646/150‑1 (MQ=255)
                                                                                              tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                  >  2:3511018/1‑150 (MQ=255)
                                                                                              tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                  <  1:2027101/150‑1 (MQ=255)
                                                                                               aCTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAc                                                 <  1:4232058/150‑1 (MQ=255)
                                                                                               aCTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTAGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAc                                                 >  1:1505917/1‑150 (MQ=255)
                                                                                                 ttCGCTGATCGCACCGTTCCTTTTCCCTAATATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGAATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATCTAAAGCCACAACGGGTTCGTAAACTg                                               >  1:1481051/1‑150 (MQ=255)
                                                                                                            cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    <  2:2783233/150‑1 (MQ=255)
                                                                                                            cACCGTTCCTTGTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    <  2:3830724/150‑1 (MQ=255)
                                                                                                             aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                   >  2:950151/1‑150 (MQ=255)
                                                                                                             aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                   <  1:178149/150‑1 (MQ=255)
                                                                                                               cGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAt                                 <  2:1834825/150‑1 (MQ=255)
                                                                                                                gTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                >  2:4440275/1‑150 (MQ=255)
                                                                                                                 ttCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTACATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                               >  2:316738/1‑150 (MQ=255)
                                                                                                                 ttCCTATTCCCGATTATTCTGGCAGTAAAGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                               >  2:2725506/1‑150 (MQ=255)
                                                                                                                  tCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                              >  2:2118559/1‑150 (MQ=255)
                                                                                                                      tttCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATg                          >  1:569520/1‑150 (MQ=255)
                                                                                                                       ttCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGa                         >  1:198925/1‑150 (MQ=255)
                                                                                                                        tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                        <  1:3328306/150‑1 (MQ=255)
                                                                                                                         cccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg                       <  1:2447251/150‑1 (MQ=255)
                                                                                                                         cccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg                       <  2:4347610/150‑1 (MQ=255)
                                                                                                                         cccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg                       <  2:1518220/150‑1 (MQ=255)
                                                                                                                         cccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg                       <  1:132436/150‑1 (MQ=255)
                                                                                                                          ccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc                      <  2:768250/150‑1 (MQ=255)
                                                                                                                          ccGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc                      <  1:671468/150‑1 (MQ=255)
                                                                                                                           cGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCa                     >  1:1249025/1‑150 (MQ=255)
                                                                                                                            gATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCaa                    >  2:1628082/1‑150 (MQ=255)
                                                                                                                            gATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCaa                    <  2:1738944/150‑1 (MQ=255)
                                                                                                                            gATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCaa                    <  1:2585243/150‑1 (MQ=255)
                                                                                                                             attattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAAc                   <  1:4138385/150‑1 (MQ=255)
                                                                                                                               tattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc                 <  1:4240493/150‑1 (MQ=255)
                                                                                                                                attCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGcc                >  2:1731474/1‑150 (MQ=255)
                                                                                                                                 ttCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCa               >  2:2270988/1‑150 (MQ=255)
                                                                                                                                   cTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATg             <  2:2342588/150‑1 (MQ=255)
                                                                                                                                   cTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATg             <  2:4266792/150‑1 (MQ=255)
                                                                                                                                    tGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc            >  1:2127939/1‑150 (MQ=255)
                                                                                                                                    tGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc            >  1:3783150/1‑150 (MQ=255)
                                                                                                                                     ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa           >  1:4516974/1‑150 (MQ=255)
                                                                                                                                       cAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACCGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc         <  1:1045098/150‑1 (MQ=255)
                                                                                                                                           taTGGGTTTAAATTTGCGATGCGTCGCTCATTTTTGATTTATGTGTCACGCGTTTCATAATTAATGAGATTCAGATCACATATAATGCCACAAAGGGTTGGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCTTGCATCCACg     <  2:4126662/149‑1 (MQ=255)
                                                                                                                                           aaTGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACg     <  2:3829440/150‑1 (MQ=255)
                                                                                                                                             tGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt   <  2:4215255/150‑1 (MQ=255)
                                                                                                                                             tGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt   <  2:3506107/150‑1 (MQ=255)
                                                                                                                                              gggTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt  <  2:452226/150‑1 (MQ=255)
                                                                                                                                              gggTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt  <  2:2383430/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225045‑2225336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGT  >  CP009273/2225042‑2225334
                                                                                                                                                    |                                                                                                                                                
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTT                                                                                                                                                 <  SRR19973072.2196449/150‑1 (MQ=60)
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTT                                                                                                                                                 <  SRR19973072.3708717/150‑1 (MQ=60)
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTT                                                                                                                                                 <  SRR19973072.2196966/150‑1 (MQ=60)
   CTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAA                                                                                                                                              >  SRR19973072.3034854/1‑150 (MQ=60)
      CCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATT                                                                                                                                           >  SRR19973072.568864/1‑150 (MQ=60)
      CCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATT                                                                                                                                           >  SRR19973072.4276730/1‑150 (MQ=60)
         CGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGC                                                                                                                                        >  SRR19973072.4569021/1‑150 (MQ=60)
         CGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGC                                                                                                                                        <  SRR19973072.4439176/150‑1 (MQ=60)
         CGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGC                                                                                                                                        <  SRR19973072.4455767/150‑1 (MQ=60)
         CGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTACGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGC                                                                                                                                        >  SRR19973072.4569001/1‑150 (MQ=60)
          GCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCG                                                                                                                                       >  SRR19973072.650318/1‑150 (MQ=60)
          GCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCG                                                                                                                                       >  SRR19973072.761398/1‑150 (MQ=60)
               CCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCG                                                                                                                                  >  SRR19973072.3808517/1‑150 (MQ=60)
                 CTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTC                                                                                                                                <  SRR19973072.1381239/150‑1 (MQ=60)
                 CTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTC                                                                                                                                <  SRR19973072.3642581/150‑1 (MQ=60)
                      TACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCA                                                                                                                           <  SRR19973072.2861458/150‑1 (MQ=60)
                       ACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAT                                                                                                                          <  SRR19973072.3392332/150‑1 (MQ=60)
                         GGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTT                                                                                                                        <  SRR19973072.2501542/150‑1 (MQ=60)
                         GGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTT                                                                                                                        >  SRR19973072.2773464/1‑150 (MQ=60)
                             CGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGA                                                                                                                    <  SRR19973072.3851631/150‑1 (MQ=60)
                              GCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGAT                                                                                                                   <  SRR19973072.640231/150‑1 (MQ=60)
                              GCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGAT                                                                                                                   <  SRR19973072.640258/150‑1 (MQ=60)
                              GCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGAT                                                                                                                   <  SRR19973072.640286/150‑1 (MQ=60)
                               CTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATG                                                                                                                  <  SRR19973072.2485122/150‑1 (MQ=60)
                                TGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGT                                                                                                                 >  SRR19973072.3795508/1‑150 (MQ=60)
                                TGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGT                                                                                                                 >  SRR19973072.89696/1‑150 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                               <  SRR19973072.4597249/150‑1 (MQ=60)
                                    CCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGT                                                                                                             >  SRR19973072.3142780/1‑150 (MQ=60)
                                    CCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGT                                                                                                             >  SRR19973072.3142781/1‑150 (MQ=60)
                                     CGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTT                                                                                                            <  SRR19973072.2516180/150‑1 (MQ=60)
                                      GAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTT                                                                                                           >  SRR19973072.2714979/1‑150 (MQ=60)
                                      GAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTT                                                                                                           >  SRR19973072.1961088/1‑150 (MQ=60)
                                      GAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTT                                                                                                           >  SRR19973072.1961000/1‑150 (MQ=60)
                                        GCTGGATTATCAGGAAGGTGCATTTAGTTCNCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAATTTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCA                                                                                                         >  SRR19973072.4295159/1‑150 (MQ=60)
                                         CTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAC                                                                                                        >  SRR19973072.4094878/1‑150 (MQ=60)
                                         CTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAC                                                                                                        >  SRR19973072.1742749/1‑150 (MQ=60)
                                         CTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAC                                                                                                        >  SRR19973072.1742628/1‑150 (MQ=60)
                                         CTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAC                                                                                                        <  SRR19973072.1566551/150‑1 (MQ=60)
                                          TGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACG                                                                                                       >  SRR19973072.1911541/1‑150 (MQ=60)
                                                ATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGC                                                                                                 >  SRR19973072.3344542/1‑150 (MQ=60)
                                                ATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGC                                                                                                 >  SRR19973072.1760773/1‑150 (MQ=60)
                                                 TCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCA                                                                                                <  SRR19973072.4639002/150‑1 (MQ=60)
                                                      AAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATT                                                                                           <  SRR19973072.4093723/150‑1 (MQ=60)
                                                      AAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATT                                                                                           >  SRR19973072.698324/1‑150 (MQ=60)
                                                        GGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAA                                                                                         <  SRR19973072.2083844/150‑1 (MQ=60)
                                                          TGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATG                                                                                       <  SRR19973072.3262457/150‑1 (MQ=60)
                                                           GCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGAATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGA                                                                                      <  SRR19973072.930433/150‑1 (MQ=60)
                                                            CATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAG                                                                                     <  SRR19973072.4355961/150‑1 (MQ=60)
                                                               TTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATT                                                                                  <  SRR19973072.2157823/150‑1 (MQ=60)
                                                                TAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTC                                                                                 >  SRR19973072.2257971/1‑150 (MQ=60)
                                                                TAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTC                                                                                 >  SRR19973072.1275370/1‑150 (MQ=60)
                                                                TAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTC                                                                                 >  SRR19973072.1275383/1‑150 (MQ=60)
                                                                  GTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAG                                                                               <  SRR19973072.3180929/150‑1 (MQ=60)
                                                                     CGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATC                                                                            >  SRR19973072.810950/1‑150 (MQ=60)
                                                                      GCTAGCTCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCA                                                                           <  SRR19973072.2001211/150‑1 (MQ=60)
                                                                      GCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCA                                                                           <  SRR19973072.4056866/150‑1 (MQ=60)
                                                                         AGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACAT                                                                        >  SRR19973072.2353741/1‑150 (MQ=60)
                                                                           CGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATAT                                                                      >  SRR19973072.1539904/1‑150 (MQ=60)
                                                                           CGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATAT                                                                      >  SRR19973072.3286847/1‑150 (MQ=60)
                                                                             CTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAA                                                                    <  SRR19973072.3515541/150‑1 (MQ=60)
                                                                                GTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGC                                                                 >  SRR19973072.4212886/1‑150 (MQ=60)
                                                                                GTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGC                                                                 >  SRR19973072.3961767/1‑150 (MQ=60)
                                                                                GTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGC                                                                 <  SRR19973072.86236/150‑1 (MQ=60)
                                                                                 TGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCC                                                                <  SRR19973072.2921103/150‑1 (MQ=60)
                                                                                 TGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCC                                                                <  SRR19973072.4407439/150‑1 (MQ=60)
                                                                                  GTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCA                                                               >  SRR19973072.490211/1‑150 (MQ=60)
                                                                                  GTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCA                                                               >  SRR19973072.3772832/1‑150 (MQ=60)
                                                                                   TTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCAC                                                              <  SRR19973072.3364630/150‑1 (MQ=60)
                                                                                     ATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAA                                                            >  SRR19973072.2633662/1‑150 (MQ=60)
                                                                                     ATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAA                                                            <  SRR19973072.1698383/150‑1 (MQ=60)
                                                                                      TGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAC                                                           <  SRR19973072.3918815/150‑1 (MQ=60)
                                                                                      TGCGGGATAATTACTTCGCTGATCGCACAGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAC                                                           <  SRR19973072.3918828/150‑1 (MQ=60)
                                                                                        CGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGG                                                         >  SRR19973072.2379229/1‑150 (MQ=60)
                                                                                         GGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGG                                                        <  SRR19973072.282190/150‑1 (MQ=60)
                                                                                            ATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTC                                                     <  SRR19973072.4341653/150‑1 (MQ=60)
                                                                                                TTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAA                                                 <  SRR19973072.2716473/150‑1 (MQ=60)
                                                                                                 TACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAA                                                <  SRR19973072.3043411/150‑1 (MQ=60)
                                                                                                 TACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAA                                                <  SRR19973072.2031490/150‑1 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                               <  SRR19973072.3031563/150‑1 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                               >  SRR19973072.1509120/1‑150 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                               <  SRR19973072.4240877/150‑1 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                               <  SRR19973072.4240785/150‑1 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTAGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                               >  SRR19973072.1509124/1‑150 (MQ=60)
                                                                                                    TTCGCTGATCGCACCGTTCCTTTTCCCTAATATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGAATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATCTAAAGCCACAACGGGTTCGTAAACTG                                             >  SRR19973072.1484215/1‑150 (MQ=60)
                                                                                                        CTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTAT                                         >  SRR19973072.2380041/1‑150 (MQ=60)
                                                                                                        CTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCACATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTAT                                         >  SRR19973072.349538/1‑150 (MQ=60)
                                                                                                             CGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAT                                    >  SRR19973072.2620404/1‑150 (MQ=60)
                                                                                                              GCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATT                                   >  SRR19973072.3631260/1‑150 (MQ=60)
                                                                                                                ACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAC                                 <  SRR19973072.178602/150‑1 (MQ=60)
                                                                                                                  CGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAT                               >  SRR19973072.2956310/1‑150 (MQ=60)
                                                                                                                  CGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAT                               <  SRR19973072.1500612/150‑1 (MQ=60)
                                                                                                                       CTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTA                          <  SRR19973072.1144181/150‑1 (MQ=60)
                                                                                                                       CTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTA                          <  SRR19973072.3768823/150‑1 (MQ=60)
                                                                                                                         TTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATG                        >  SRR19973072.570849/1‑150 (MQ=60)
                                                                                                                         TTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATG                        >  SRR19973072.570848/1‑150 (MQ=60)
                                                                                                                         TTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATG                        >  SRR19973072.2290972/1‑150 (MQ=60)
                                                                                                                          TTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGA                       >  SRR19973072.199429/1‑150 (MQ=60)
                                                                                                                          TTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGA                       >  SRR19973072.199428/1‑150 (MQ=60)
                                                                                                                           TCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAG                      >  SRR19973072.1836184/1‑150 (MQ=60)
                                                                                                                           TCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAG                      <  SRR19973072.3335373/150‑1 (MQ=60)
                                                                                                                            CCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGG                     <  SRR19973072.2452573/150‑1 (MQ=60)
                                                                                                                            CCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGG                     <  SRR19973072.132769/150‑1 (MQ=60)
                                                                                                                             CCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGC                    <  SRR19973072.672967/150‑1 (MQ=60)
                                                                                                                             CCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGC                    <  SRR19973072.2224633/150‑1 (MQ=60)
                                                                                                                              CGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCA                   >  SRR19973072.1251705/1‑150 (MQ=60)
                                                                                                                               GATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAA                  <  SRR19973072.2590824/150‑1 (MQ=60)
                                                                                                                                ATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAAC                 <  SRR19973072.4147068/150‑1 (MQ=60)
                                                                                                                                  TATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGC               <  SRR19973072.4249320/150‑1 (MQ=60)
                                                                                                                                  TATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGC               <  SRR19973072.4249314/150‑1 (MQ=60)
                                                                                                                                    TTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCA             <  SRR19973072.687351/150‑1 (MQ=60)
                                                                                                                                    TTCTGGCAGAAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTACACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGATGCAACGCCA             >  SRR19973072.1774670/1‑150 (MQ=60)
                                                                                                                                       TGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGC          >  SRR19973072.3791083/1‑150 (MQ=60)
                                                                                                                                       TGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGC          >  SRR19973072.2132575/1‑150 (MQ=60)
                                                                                                                                        GGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCA         >  SRR19973072.4526508/1‑150 (MQ=60)
                                                                                                                                          CAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATaatcatgtaatgggataacag       <  SRR19973072.4600485/150‑22 (MQ=60)
                                                                                                                                          CAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACCGTTATCCCATTACATGATTATGAGGCAACGCCATGCATC       <  SRR19973072.1047350/150‑1 (MQ=60)
                                                                                                                                             TAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCAC    >  SRR19973072.3351440/1‑150 (MQ=60)
                                                                                                                                               ATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGT  <  SRR19973072.2483981/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGT  >  CP009273/2225042‑2225334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑