Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,225,190 C→T 79.7% G231G (GGC→GGT)  yohK → LrgB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,225,1900CT79.7% 561.4 / 141.6 243G231G (GGC→GGT) yohKLrgB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (28/21);  new base T (80/113);  total (109/134)
Fisher's exact test for biased strand distribution p-value = 5.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 244 total aligned reads displayed.

GACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCA  >  CP009273/2225043‑2225339
                                                                                                                                                   |                                                                                                                                                     
   tGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaaa                                                                                                                                                  >  1:3076551/1‑150 (MQ=255)
     ccTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAAtt                                                                                                                                                <  1:1696862/150‑1 (MQ=255)
         gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCg                                                                                                                                            <  2:1606587/150‑1 (MQ=255)
         gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCg                                                                                                                                            <  2:1606935/150‑1 (MQ=255)
          cACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCAGTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGa                                                                                                                                           >  2:1391578/1‑150 (MQ=255)
           aCGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGAt                                                                                                                                          <  2:2538421/150‑1 (MQ=255)
            cGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                         >  1:665036/1‑150 (MQ=255)
            cGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                         <  2:1533591/150‑1 (MQ=255)
             gCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGc                                                                                                                                        >  2:289099/1‑150 (MQ=255)
                   ggTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTcgcg                                                                                                                                  >  2:101104/1‑150 (MQ=255)
                          cgcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttttt                                                                                                                           <  2:26486/150‑1 (MQ=255)
                           gcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTg                                                                                                                          <  2:1479645/150‑1 (MQ=255)
                             gcTGCGCCGAGCTTGATTATCAGGAAGGTTCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                        <  2:881215/150‑1 (MQ=255)
                             gcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                        <  2:1378652/150‑1 (MQ=255)
                             gcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                        <  2:788613/150‑1 (MQ=255)
                              cTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatg                                                                                                                       <  1:2527928/150‑1 (MQ=255)
                              cTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatg                                                                                                                       <  2:3580772/150‑1 (MQ=255)
                              cTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGatg                                                                                                                       >  1:3832015/1‑150 (MQ=255)
                                gcgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgta                                                                                                                     <  2:1739766/150‑1 (MQ=255)
                                gcgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgta                                                                                                                     <  1:4138761/150‑1 (MQ=255)
                                 cgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGatgtat                                                                                                                    >  1:1553041/1‑150 (MQ=255)
                                 cgcCGAGCTGGATTATCAGGAAGGTACATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtat                                                                                                                    <  1:843456/150‑1 (MQ=255)
                                  gcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtatg                                                                                                                   <  2:2299872/150‑1 (MQ=255)
                                     gAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGttt                                                                                                                >  2:915143/1‑150 (MQ=255)
                                     gAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGttt                                                                                                                >  1:3969819/1‑150 (MQ=255)
                                      aGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTc                                                                                                               >  1:3064869/1‑150 (MQ=255)
                                      aGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTc                                                                                                               <  1:2737724/150‑1 (MQ=255)
                                          ggATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgc                                                                                                           >  2:260406/1‑150 (MQ=255)
                                           gatgatCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                          <  2:4299294/150‑1 (MQ=255)
                                           gATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                          >  2:2036418/1‑150 (MQ=255)
                                           gATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                          <  2:1092278/150‑1 (MQ=255)
                                           gATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                          >  1:1957842/1‑150 (MQ=255)
                                            aTTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGt                                                                                                         >  1:1162174/1‑150 (MQ=255)
                                                 cAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAt                                                                                                    >  1:1725854/1‑150 (MQ=255)
                                                  aGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAta                                                                                                   >  1:800628/1‑150 (MQ=255)
                                                  aGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAta                                                                                                   <  2:4373842/150‑1 (MQ=255)
                                                   ggAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaa                                                                                                  <  2:3805449/150‑1 (MQ=255)
                                                    gAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaat                                                                                                 <  1:2964491/150‑1 (MQ=255)
                                                     aaGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatt                                                                                                >  2:4007278/1‑150 (MQ=255)
                                                      aGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatta                                                                                               <  2:422850/150‑1 (MQ=255)
                                                       ggTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaa                                                                                              <  1:1839136/150‑1 (MQ=255)
                                                       ggTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaa                                                                                              >  2:317843/1‑150 (MQ=255)
                                                        gTGCATTTAGTTCGCTAGCGCTTGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaat                                                                                             <  1:2479908/150‑1 (MQ=255)
                                                         tGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATg                                                                                            <  2:3007533/150‑1 (MQ=255)
                                                           cATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgag                                                                                          <  2:3621543/150‑1 (MQ=255)
                                                           cATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgag                                                                                          >  1:1249993/1‑150 (MQ=255)
                                                            aTTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgaga                                                                                         >  2:1263385/1‑150 (MQ=255)
                                                              ttAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAtt                                                                                       <  2:3818542/150‑1 (MQ=255)
                                                                     gCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATca                                                                                >  2:418365/1‑150 (MQ=255)
                                                                          cgcTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCATTGCATAATTAATGAGATTCAGATCACatat                                                                           >  1:2406539/1‑150 (MQ=255)
                                                                          cgcTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCATTGCATAATTAATGAGATTCAGATCACatat                                                                           >  1:3532440/1‑150 (MQ=255)
                                                                            cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                         >  2:3510573/1‑150 (MQ=255)
                                                                            cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                         <  1:2832751/150‑1 (MQ=255)
                                                                            cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                         <  1:4419448/150‑1 (MQ=255)
                                                                            cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                         >  2:398026/1‑150 (MQ=255)
                                                                             tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                        <  1:1431996/150‑1 (MQ=255)
                                                                             tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                        >  1:2107220/1‑150 (MQ=255)
                                                                              ggTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAg                                                                       <  2:3206634/150‑1 (MQ=255)
                                                                              ggTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAg                                                                       >  1:331422/1‑150 (MQ=255)
                                                                              ggTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAg                                                                       >  2:4074804/1‑150 (MQ=255)
                                                                                tgtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGcc                                                                     >  1:3952743/1‑150 (MQ=255)
                                                                                 gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                    <  1:174394/150‑1 (MQ=255)
                                                                                  ttATGCGGGATAATTACGTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATTGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCTTAATTAATGAGATTCAGATTACATATAAAGCcac                                                                   <  1:3228521/150‑1 (MQ=255)
                                                                                   tATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCcaca                                                                  >  1:2639371/1‑150 (MQ=255)
                                                                                     tGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                                <  2:4495107/150‑1 (MQ=255)
                                                                                     tGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                                <  1:4384418/150‑1 (MQ=255)
                                                                                        gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACggg                                                             >  2:1546711/1‑150 (MQ=255)
                                                                                                tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTaaa                                                     >  2:1746775/1‑150 (MQ=255)
                                                                                                tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                     >  2:3221307/1‑150 (MQ=255)
                                                                                                tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                     >  2:3400236/1‑150 (MQ=255)
                                                                                                tACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaaa                                                     >  2:2299996/1‑150 (MQ=255)
                                                                                                 aCTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAc                                                    >  1:1640505/1‑150 (MQ=255)
                                                                                                       cTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTAt                                              <  1:3625229/150‑1 (MQ=255)
                                                                                                         gATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCCTTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATcc                                            >  2:1228983/1‑150 (MQ=255)
                                                                                                            cGCACCGGTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAt                                         <  1:4276487/150‑1 (MQ=255)
                                                                                                             gCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAtc                                        <  2:2095741/150‑2 (MQ=255)
                                                                                                             gCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTCGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAtt                                        <  1:174662/150‑1 (MQ=255)
                                                                                                             gCACCGGTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAtt                                        <  2:3655367/150‑1 (MQ=255)
                                                                                                              cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                       <  1:824690/150‑1 (MQ=255)
                                                                                                               aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                      <  1:3934310/150‑1 (MQ=255)
                                                                                                                ccGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACa                                     <  1:2841772/150‑1 (MQ=255)
                                                                                                                ccGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACa                                     >  2:4318360/1‑150 (MQ=255)
                                                                                                                 cGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAt                                    >  1:3817165/1‑150 (MQ=255)
                                                                                                                  ggtCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                   <  1:3947656/148‑1 (MQ=255)
                                                                                                                  gTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                   <  1:2292555/150‑1 (MQ=255)
                                                                                                                  gTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                   <  1:133741/150‑1 (MQ=255)
                                                                                                                   ttCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                                  >  1:1698496/1‑150 (MQ=255)
                                                                                                                    tCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                                 <  1:1000394/150‑1 (MQ=255)
                                                                                                                     ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAtt                                <  2:807459/150‑1 (MQ=255)
                                                                                                                          tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                           <  2:1092723/150‑1 (MQ=255)
                                                                                                                                ttattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACg                     >  2:3872398/1‑150 (MQ=255)
                                                                                                                                       ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa              >  1:4025690/1‑150 (MQ=255)
                                                                                                                                         cAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc            >  1:4114186/1‑150 (MQ=255)
                                                                                                                                          aGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATcc           >  2:2214414/1‑150 (MQ=255)
                                                                                                                                           gTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa          >  1:457135/1‑150 (MQ=255)
                                                                                                                                            tAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCAc         <  2:4251407/150‑1 (MQ=255)
                                                                                                                                              aTGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGt       >  1:2139732/1‑150 (MQ=255)
                                                                                                                                               tGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt      >  1:2569707/1‑150 (MQ=255)
                                                                                                                                                gggCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt     <  1:4242521/150‑1 (MQ=255)
                                                                                                                                                gggCTAAAATGTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt     <  2:2463946/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCA  >  CP009273/2225043‑2225339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCA  >  CP009273/2225042‑2225339
                                                                                                                                                    |                                                                                                                                                     
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTT                                                                                                                                                      <  SRR19973080.1263328/150‑1 (MQ=60)
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTT                                                                                                                                                      <  SRR19973080.1263354/150‑1 (MQ=60)
    TGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAA                                                                                                                                                  >  SRR19973080.3093332/1‑150 (MQ=60)
    TGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAA                                                                                                                                                  >  SRR19973080.3092486/1‑150 (MQ=60)
      CCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATT                                                                                                                                                <  SRR19973080.1706741/150‑1 (MQ=60)
      CCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATT                                                                                                                                                >  SRR19973080.3577274/1‑150 (MQ=60)
      CCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATT                                                                                                                                                <  SRR19973080.1706738/150‑1 (MQ=60)
         CGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGC                                                                                                                                             <  SRR19973080.4275087/150‑1 (MQ=60)
             CGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATG                                                                                                                                         >  SRR19973080.669007/1‑150 (MQ=60)
             CGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATG                                                                                                                                         >  SRR19973080.2061143/1‑150 (MQ=60)
                  TCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCG                                                                                                                                    >  SRR19973080.1660088/1‑150 (MQ=60)
                  TCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCG                                                                                                                                    >  SRR19973080.1660075/1‑150 (MQ=60)
                           CGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTT                                                                                                                           <  SRR19973080.2505782/150‑1 (MQ=60)
                               CTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATG                                                                                                                       <  SRR19973080.2541825/150‑1 (MQ=60)
                               CTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATG                                                                                                                       <  SRR19973080.2541086/150‑1 (MQ=60)
                               CTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATG                                                                                                                       >  SRR19973080.1215862/1‑150 (MQ=60)
                               CTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATG                                                                                                                       >  SRR19973080.3853054/1‑150 (MQ=60)
                                 GCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTA                                                                                                                     <  SRR19973080.4161152/150‑1 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                                    >  SRR19973080.1562044/1‑150 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                                    >  SRR19973080.2549930/1‑150 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTACATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                                    <  SRR19973080.848521/150‑1 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTACATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                                    <  SRR19973080.848541/150‑1 (MQ=60)
                                  CGCCGAGCTGGATTATCAGGAAGGTACATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTAT                                                                                                                    <  SRR19973080.848550/150‑1 (MQ=60)
                                   GCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATG                                                                                                                   <  SRR19973080.4026074/150‑1 (MQ=60)
                                   GCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATG                                                                                                                   <  SRR19973080.4209663/150‑1 (MQ=60)
                                      GAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTT                                                                                                                >  SRR19973080.3991669/1‑150 (MQ=60)
                                      GAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTT                                                                                                                >  SRR19973080.2429901/1‑150 (MQ=60)
                                       AGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTC                                                                                                               <  SRR19973080.1332647/150‑1 (MQ=60)
                                       AGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTC                                                                                                               <  SRR19973080.2752784/150‑1 (MQ=60)
                                       AGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTC                                                                                                               >  SRR19973080.3081537/1‑150 (MQ=60)
                                       AGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTC                                                                                                               <  SRR19973080.1989321/150‑1 (MQ=60)
                                            GATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCG                                                                                                          >  SRR19973080.824538/1‑150 (MQ=60)
                                            GATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCG                                                                                                          >  SRR19973080.1968937/1‑150 (MQ=60)
                                             ATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGT                                                                                                         >  SRR19973080.1169062/1‑150 (MQ=60)
                                                  CAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAT                                                                                                    >  SRR19973080.1735843/1‑150 (MQ=60)
                                                  CAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAT                                                                                                    <  SRR19973080.2705860/150‑1 (MQ=60)
                                                   AGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATA                                                                                                   >  SRR19973080.805315/1‑150 (MQ=60)
                                                   AGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATA                                                                                                   >  SRR19973080.3976120/1‑150 (MQ=60)
                                                   AGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATA                                                                                                   >  SRR19973080.686117/1‑150 (MQ=60)
                                                   AGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATA                                                                                                   >  SRR19973080.3977630/1‑150 (MQ=60)
                                                     GAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAAT                                                                                                 <  SRR19973080.2980542/150‑1 (MQ=60)
                                                        GGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAA                                                                                              <  SRR19973080.1849492/150‑1 (MQ=60)
                                                        GGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAA                                                                                              <  SRR19973080.1848844/150‑1 (MQ=60)
                                                         GTGCATTTAGTTCGCTAGCGCTTGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAAT                                                                                             <  SRR19973080.2493623/150‑1 (MQ=60)
                                                            CATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAG                                                                                          >  SRR19973080.1258751/1‑150 (MQ=60)
                                                            CATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAG                                                                                          >  SRR19973080.1257564/1‑150 (MQ=60)
                                                            CATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAG                                                                                          >  SRR19973080.3550924/1‑150 (MQ=60)
                                                                  GTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAG                                                                                    <  SRR19973080.3958624/150‑1 (MQ=60)
                                                                  GTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAG                                                                                    <  SRR19973080.3958455/150‑1 (MQ=60)
                                                                  GTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAG                                                                                    <  SRR19973080.1476524/150‑1 (MQ=60)
                                                                     CGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATC                                                                                 <  SRR19973080.3340646/150‑1 (MQ=60)
                                                                     CGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATC                                                                                 <  SRR19973080.2834127/150‑1 (MQ=60)
                                                                     CGCTAGCGCTGGTGTGATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATC                                                                                 <  SRR19973080.2834287/150‑1 (MQ=60)
                                                                        TAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACA                                                                              <  SRR19973080.4070924/150‑1 (MQ=60)
                                                                         AGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACAT                                                                             <  SRR19973080.3196281/150‑1 (MQ=60)
                                                                           CGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCATTGCATAATTAATGAGATTCAGATCACATAT                                                                           >  SRR19973080.2419891/1‑150 (MQ=60)
                                                                           CGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCATTGCATAATTAATGAGATTCAGATCACATAT                                                                           >  SRR19973080.3551980/1‑150 (MQ=60)
                                                                             CTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAA                                                                         <  SRR19973080.4442724/150‑1 (MQ=60)
                                                                             CTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAA                                                                         <  SRR19973080.2848322/150‑1 (MQ=60)
                                                                             CTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAA                                                                         <  SRR19973080.4443479/150‑1 (MQ=60)
                                                                              TGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAA                                                                        <  SRR19973080.1440375/150‑1 (MQ=60)
                                                                              TGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAA                                                                        <  SRR19973080.3737613/150‑1 (MQ=60)
                                                                              TGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAA                                                                        >  SRR19973080.2119122/1‑150 (MQ=60)
                                                                               GGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAG                                                                       >  SRR19973080.333432/1‑150 (MQ=60)
                                                                                 TGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCC                                                                     >  SRR19973080.3974143/1‑150 (MQ=60)
                                                                                 TGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCC                                                                     >  SRR19973080.3974513/1‑150 (MQ=60)
                                                                                  GTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCA                                                                    <  SRR19973080.175765/150‑1 (MQ=60)
                                                                                   TTATGCGGGATAATTACGTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATTGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCTTAATTAATGAGATTCAGATTACATATAAAGCCAC                                                                   <  SRR19973080.3246315/150‑1 (MQ=60)
                                                                                    TATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACA                                                                  >  SRR19973080.2653620/1‑150 (MQ=60)
                                                                                    TATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACA                                                                  <  SRR19973080.1750007/150‑1 (MQ=60)
                                                                                    TATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACA                                                                  <  SRR19973080.2972460/150‑1 (MQ=60)
                                                                                    TATGCGAGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACA                                                                  <  SRR19973080.3265847/150‑1 (MQ=60)
                                                                                     ATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAA                                                                 <  SRR19973080.1884321/150‑1 (MQ=60)
                                                                                      TGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAC                                                                <  SRR19973080.4408309/150‑1 (MQ=60)
                                                                                      TGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAC                                                                <  SRR19973080.4408310/150‑1 (MQ=60)
                                                                                      TGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAC                                                                <  SRR19973080.1592552/150‑1 (MQ=60)
                                                                                       GCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACG                                                               <  SRR19973080.2162062/150‑1 (MQ=60)
                                                                                           GATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTT                                                           <  SRR19973080.3021513/150‑1 (MQ=60)
                                                                                            ATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTC                                                          >  SRR19973080.3093604/1‑150 (MQ=60)
                                                                                             TAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCG                                                         <  SRR19973080.4449750/150‑1 (MQ=60)
                                                                                                  ACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAC                                                    >  SRR19973080.1650111/1‑150 (MQ=60)
                                                                                                       GCTGATCGCACCGGTCCTTGTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTA                                               <  SRR19973080.2643386/150‑1 (MQ=60)
                                                                                                        CTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTAT                                              <  SRR19973080.3645262/150‑1 (MQ=60)
                                                                                                             CGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAT                                         <  SRR19973080.4300624/150‑1 (MQ=60)
                                                                                                             CGCACCGGTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAT                                         <  SRR19973080.4299838/150‑1 (MQ=60)
                                                                                                              GCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATT                                        <  SRR19973080.175692/150‑1 (MQ=60)
                                                                                                              GCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATT                                        <  SRR19973080.175846/150‑1 (MQ=60)
                                                                                                              GCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTCGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATT                                        <  SRR19973080.176035/150‑1 (MQ=60)
                                                                                                               CACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTA                                       <  SRR19973080.829606/150‑1 (MQ=60)
                                                                                                               CACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTA                                       <  SRR19973080.463915/150‑1 (MQ=60)
                                                                                                               CACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTA                                       <  SRR19973080.463897/150‑1 (MQ=60)
                                                                                                                ACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAC                                      <  SRR19973080.3956013/150‑1 (MQ=60)
                                                                                                                ACCGGTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAC                                      <  SRR19973080.2618735/150‑1 (MQ=60)
                                                                                                                 CCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACA                                     <  SRR19973080.2857374/150‑1 (MQ=60)
                                                                                                                 CCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACA                                     <  SRR19973080.2857369/150‑1 (MQ=60)
                                                                                                                  CGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAT                                    >  SRR19973080.3838952/1‑150 (MQ=60)
                                                                                                                  CGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACAT                                    >  SRR19973080.3838061/1‑150 (MQ=60)
                                                                                                                   GTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATG                                   <  SRR19973080.2305183/150‑1 (MQ=60)
                                                                                                                   GTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATG                                   <  SRR19973080.2305186/150‑1 (MQ=60)
                                                                                                                   GTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATG                                   <  SRR19973080.237273/150‑1 (MQ=60)
                                                                                                                   GTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATG                                   <  SRR19973080.134865/150‑1 (MQ=60)
                                                                                                                   GGTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATG                                   <  SRR19973080.3969401/150‑1 (MQ=60)
                                                                                                                    TTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGA                                  >  SRR19973080.1708380/1‑150 (MQ=60)
                                                                                                                    TTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGA                                  >  SRR19973080.1708403/1‑150 (MQ=60)
                                                                                                                     TCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAT                                 <  SRR19973080.1006391/150‑1 (MQ=60)
                                                                                                                         TTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATG                             <  SRR19973080.2911644/150‑1 (MQ=60)
                                                                                                                                     TCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCAT                 <  SRR19973080.1442398/150‑1 (MQ=60)
                                                                                                                                       TGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGC               >  SRR19973080.930056/1‑150 (MQ=60)
                                                                                                                                       TGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGGTGCATAATTAATGAGATTCAGATCAGATATAAAGCCACAACGGGTTCGGAAACTGTTATCCCATTACCTGATTATGAGGCAACGCCATGC               >  SRR19973080.3868027/1‑150 (MQ=60)
                                                                                                                                        GGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCA              >  SRR19973080.4047763/1‑150 (MQ=60)
                                                                                                                                        GGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCA              >  SRR19973080.4047752/1‑150 (MQ=60)
                                                                                                                                        GGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCA              <  SRR19973080.807316/150‑1 (MQ=60)
                                                                                                                                          CAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATC            >  SRR19973080.4136520/1‑150 (MQ=60)
                                                                                                                                          CAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATC            >  SRR19973080.4136519/1‑150 (MQ=60)
                                                                                                                                           AGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCC           >  SRR19973080.1422638/1‑150 (MQ=60)
                                                                                                                                            GTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCA          >  SRR19973080.460012/1‑150 (MQ=60)
                                                                                                                                            GTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCA          >  SRR19973080.182922/1‑150 (MQ=60)
                                                                                                                                             TAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCAC         >  SRR19973080.3650946/1‑150 (MQ=60)
                                                                                                                                               ATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGT       >  SRR19973080.2151685/1‑150 (MQ=60)
                                                                                                                                               ATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGT       >  SRR19973080.2151751/1‑150 (MQ=60)
                                                                                                                                                TGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTT      >  SRR19973080.2583740/1‑150 (MQ=60)
                                                                                                                                                 GGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT     <  SRR19973080.4265280/150‑1 (MQ=60)
                                                                                                                                                 GGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT     <  SRR19973080.4265668/150‑1 (MQ=60)
                                                                                                                                                  GGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTT    <  SRR19973080.52736/150‑1 (MQ=60)
                                                                                                                                                   GTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATTACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTC   <  SRR19973080.3158545/150‑1 (MQ=60)
                                                                                                                                                    CTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCA  <  SRR19973080.102019/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCA  >  CP009273/2225042‑2225339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑