Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,412,692 G→T 33.4% intergenic (‑37/+21) yfcD ← / ← yfcE putative NUDIX hydrolase/phosphodiesterase activity on bis‑pNPP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,412,6920GT33.4% 421.6 / 210.7 233intergenic (‑37/+21)yfcD/yfcEputative NUDIX hydrolase/phosphodiesterase activity on bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base G (74/81);  new base T (34/44);  total (108/125)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 234 total aligned reads displayed.

ATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTT  >  CP009273/2412545‑2412841
                                                                                                                                                   |                                                                                                                                                     
aTGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCa                                                                                                                                                     <  2:621045/150‑1 (MQ=255)
  gACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCAtt                                                                                                                                                   <  2:1084/150‑1 (MQ=255)
   aCGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCAttt                                                                                                                                                  <  2:694168/150‑1 (MQ=255)
   aCGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCAttt                                                                                                                                                  <  1:3392143/150‑1 (MQ=255)
      cAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGa                                                                                                                                               >  2:3569277/1‑150 (MQ=255)
      cAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGa                                                                                                                                               >  2:2937634/1‑150 (MQ=255)
       aGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAg                                                                                                                                              <  1:2862985/150‑1 (MQ=255)
         acacTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGtt                                                                                                                                            >  1:4590225/1‑150 (MQ=255)
          cacTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTtg                                                                                                                                           >  1:883679/1‑150 (MQ=255)
             tgtgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTgg                                                                                                                                        <  2:1867946/150‑1 (MQ=255)
             tgtgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTgg                                                                                                                                        >  1:375964/1‑150 (MQ=255)
             tgtgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTgg                                                                                                                                        >  1:2694129/1‑150 (MQ=255)
               tgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGt                                                                                                                                      >  1:550411/1‑150 (MQ=255)
               tgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGt                                                                                                                                      <  2:4628988/150‑1 (MQ=255)
               tgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGt                                                                                                                                      >  1:4127903/1‑150 (MQ=255)
               tgCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGt                                                                                                                                      <  2:4355410/150‑1 (MQ=255)
                gCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTa                                                                                                                                     <  2:1208869/150‑1 (MQ=255)
                gCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTa                                                                                                                                     <  2:4480235/150‑1 (MQ=255)
                 cccGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTaa                                                                                                                                    <  2:1352433/150‑1 (MQ=255)
                  ccGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTaaa                                                                                                                                   <  1:4233304/150‑1 (MQ=255)
                   cGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAAt                                                                                                                                  <  1:1016198/150‑1 (MQ=255)
                    gCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAAtt                                                                                                                                 >  2:2579729/1‑150 (MQ=255)
                    gCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAAtt                                                                                                                                 >  2:4044721/1‑150 (MQ=255)
                           ttCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGAtt                                                                                                                          <  2:2004142/150‑1 (MQ=255)
                            tCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTa                                                                                                                         >  2:3433592/1‑150 (MQ=255)
                                   tggctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGa                                                                                                                  <  1:1706639/150‑1 (MQ=255)
                                   tggctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGa                                                                                                                  <  1:3059561/150‑1 (MQ=255)
                                      ctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCt                                                                                                               <  2:636021/150‑1 (MQ=255)
                                      ctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCt                                                                                                               <  1:1987427/150‑1 (MQ=255)
                                         gtgCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTgcg                                                                                                            <  2:2607252/150‑1 (MQ=255)
                                          tgCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTgcgc                                                                                                           <  2:3654398/150‑1 (MQ=255)
                                               tGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGa                                                                                                      <  2:2500359/150‑1 (MQ=255)
                                               tGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGa                                                                                                      <  2:2500529/150‑1 (MQ=255)
                                                gACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGAt                                                                                                     >  1:4071226/1‑150 (MQ=255)
                                                gACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGAt                                                                                                     <  1:1514832/150‑1 (MQ=255)
                                                gACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGAt                                                                                                     >  1:2011679/1‑150 (MQ=255)
                                                gACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGAt                                                                                                     <  1:2131381/150‑1 (MQ=255)
                                                 aCTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTAATTGCAAATAGACGCTGTTACATTATCAGGAAACCTTCTTTTTCTGTCGATCTTACGGCGCATTTTGAGTTGTGTGTAAATTAAGGATTAATCGCGACCTTCGAAATGATa                                                                                                    <  2:3963206/150‑1 (MQ=255)
                                                  cTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATAc                                                                                                   >  1:1440622/1‑150 (MQ=255)
                                                     cGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                                                                <  2:4256942/150‑1 (MQ=255)
                                                     cGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCAAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTATGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                                                                <  2:2068673/150‑1 (MQ=255)
                                                      gTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTg                                                                                               >  2:4313220/1‑150 (MQ=255)
                                                      gTTCTCTTCATTGACAATATCCACCAATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCGTACGGCGCATTTTGATTTGTGTGTAAATTACGGATTAATCTCGACCTGCGCAATGATACTTTg                                                                                               <  2:16094/150‑1 (MQ=255)
                                                       ttCTATTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTAATGATTAATCGCGACCTGCGCAATGATACTTTGa                                                                                              >  2:593940/1‑150 (MQ=255)
                                                            ttCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAtt                                                                                         >  1:2353930/1‑150 (MQ=255)
                                                            ttCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAtt                                                                                         <  1:975421/150‑1 (MQ=255)
                                                              cATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGa                                                                                       >  2:2992008/1‑150 (MQ=255)
                                                              cATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGa                                                                                       >  1:3734655/1‑150 (MQ=255)
                                                              cATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGa                                                                                       >  2:325284/1‑150 (MQ=255)
                                                               aTTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAg                                                                                      <  2:4180932/150‑1 (MQ=255)
                                                                 tGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTg                                                                                    <  2:2675965/150‑1 (MQ=255)
                                                                  gACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGc                                                                                   >  1:1218848/1‑150 (MQ=255)
                                                                    cAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGCCCTGCGCAATGATACTTTGATCATTGAGTGCGa                                                                                 <  2:2727325/150‑1 (MQ=255)
                                                                          ccACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTacac                                                                           >  2:3368034/1‑150 (MQ=255)
                                                                             cccATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTaa                                                                        >  2:2302969/1‑150 (MQ=255)
                                                                              ccATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAg                                                                       >  1:1958022/1‑150 (MQ=255)
                                                                                 ttCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTAc                                                                    <  2:1419728/150‑1 (MQ=255)
                                                                                 ttCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATGTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTAc                                                                    >  2:490864/1‑150 (MQ=255)
                                                                                      tACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAt                                                               <  1:1352310/150‑1 (MQ=255)
                                                                                       aCTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAtt                                                              >  1:4005463/1‑150 (MQ=255)
                                                                                         ttGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTAt                                                            <  1:1920290/150‑1 (MQ=255)
                                                                                            ccAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCa                                                         <  2:3305986/150‑1 (MQ=255)
                                                                                             cAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAg                                                        >  2:764761/1‑150 (MQ=255)
                                                                                             cAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAg                                                        <  2:3311398/150‑1 (MQ=255)
                                                                                              aaaCGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGc                                                       >  1:4446825/1‑150 (MQ=255)
                                                                                               aaCGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCa                                                      >  1:2210320/1‑150 (MQ=255)
                                                                                                   acgCTGTTCCTTTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGcc                                                  <  1:3027868/150‑1 (MQ=255)
                                                                                                    cgCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCa                                                 >  1:4398020/1‑150 (MQ=255)
                                                                                                     gCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAt                                                >  1:1042066/1‑150 (MQ=255)
                                                                                                          tCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTc                                           >  1:3587930/1‑150 (MQ=255)
                                                                                                               tATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCgg                                      >  2:288400/1‑150 (MQ=255)
                                                                                                                aTCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGa                                     >  2:3348632/1‑150 (MQ=255)
                                                                                                                 tCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGAt                                    <  1:4010335/150‑1 (MQ=255)
                                                                                                                  cAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGAtt                                   >  1:2611589/1‑150 (MQ=255)
                                                                                                                     gAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTAcc                                >  1:4126777/1‑150 (MQ=255)
                                                                                                                       aaCCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGc                              >  1:4200212/1‑150 (MQ=255)
                                                                                                                         ccTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCt                            <  1:4076896/150‑1 (MQ=255)
                                                                                                                          cttcttTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCtt                           <  2:1855134/150‑1 (MQ=255)
                                                                                                                          cttcttTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCtt                           <  2:1855320/150‑1 (MQ=255)
                                                                                                                             cttTTTCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCg                        >  1:707026/1‑150 (MQ=255)
                                                                                                                             cttTTTCTGGCGCTCTTACGGCGCATTTTGAGTGGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCg                        <  2:3538166/150‑1 (MQ=255)
                                                                                                                                tttCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGaa                     >  1:1336465/1‑150 (MQ=255)
                                                                                                                                tttCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGaa                     >  2:716418/1‑150 (MQ=255)
                                                                                                                                tttCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGaa                     >  2:4578032/1‑150 (MQ=255)
                                                                                                                                tttCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGaa                     >  2:4497059/1‑150 (MQ=255)
                                                                                                                                 ttCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAAt                    >  2:3729981/1‑150 (MQ=255)
                                                                                                                                  tCTGGCGCTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATa                   >  2:2609788/1‑150 (MQ=255)
                                                                                                                                  tCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATa                   <  1:3448178/150‑1 (MQ=255)
                                                                                                                                  tCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATa                   >  1:2810060/1‑150 (MQ=255)
                                                                                                                                   cTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATAc                  >  2:517967/1‑150 (MQ=255)
                                                                                                                                   cTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATAc                  <  1:390600/150‑1 (MQ=255)
                                                                                                                                       cgcTCTTACGGCTCATTTTGAGTTGTGGGTAACTTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCAc              <  2:2029241/150‑1 (MQ=255)
                                                                                                                                        gcTCTTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCAcc             >  1:4442480/1‑150 (MQ=255)
                                                                                                                                        gcTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCAcc             <  2:819195/150‑1 (MQ=255)
                                                                                                                                         ctctTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCg            >  2:2810702/1‑150 (MQ=255)
                                                                                                                                         ctctTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCg            <  1:3655187/150‑1 (MQ=255)
                                                                                                                                          tctTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGa           >  2:1750900/1‑150 (MQ=255)
                                                                                                                                           ctTACGGCTCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAg          >  2:880240/1‑150 (MQ=255)
                                                                                                                                             tACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGcc        <  2:4108148/150‑1 (MQ=255)
                                                                                                                                              aCGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCg       >  2:3784313/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTT  >  CP009273/2412545‑2412841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A