Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,415,498 G→T 5.4% T104T (ACG→ACT)  yfcH → putative NAD‑dependent nucleotide‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,415,4980GT 5.4% 1123.1 / 25.5 296T104T (ACG→ACT) yfcHputative NAD‑dependent nucleotide‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base G (171/109);  new base T (10/6);  total (181/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 299 total aligned reads displayed.

AGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCAAAGTGA  >  CP009273/2415349‑2415647
                                                                                                                                                     |                                                                                                                                                     
  cAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGcc                                                                                                                                                     >  2:3747119/1‑150 (MQ=255)
     ccTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCAcc                                                                                                                                                  <  2:468016/150‑1 (MQ=255)
        cAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTc                                                                                                                                               <  1:4380899/150‑1 (MQ=255)
        cAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTc                                                                                                                                               >  2:2255163/1‑150 (MQ=255)
        cAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTc                                                                                                                                               <  1:2582395/150‑1 (MQ=255)
        cAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTc                                                                                                                                               >  2:387439/1‑150 (MQ=255)
          aCGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCgg                                                                                                                                             >  2:4016325/1‑150 (MQ=255)
           cGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGt                                                                                                                                            >  2:4150223/1‑150 (MQ=255)
                ttGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACTCCACCGTCGGTACTCa                                                                                                                                       >  1:2407973/1‑150 (MQ=255)
                ttGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCa                                                                                                                                       >  1:3649881/1‑150 (MQ=255)
                 tGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCAt                                                                                                                                      <  2:1314276/150‑1 (MQ=255)
                         tAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCt                                                                                                                              <  2:904103/150‑1 (MQ=255)
                          aaTCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTc                                                                                                                             >  2:562361/1‑150 (MQ=255)
                           aTCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCg                                                                                                                            <  2:1507364/150‑1 (MQ=255)
                             cAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGc                                                                                                                          <  1:1401558/150‑1 (MQ=255)
                                ccTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAAc                                                                                                                       <  1:2899210/150‑1 (MQ=255)
                                ccTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAAc                                                                                                                       <  2:3379244/150‑1 (MQ=255)
                                ccTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAAc                                                                                                                       <  2:3338251/150‑1 (MQ=255)
                                ccTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAAc                                                                                                                       <  1:1058519/150‑1 (MQ=255)
                                 cTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACg                                                                                                                      >  2:967953/1‑150 (MQ=255)
                                  tGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACgg                                                                                                                     >  1:1633843/1‑150 (MQ=255)
                                     ccGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCt                                                                                                                  >  1:1024868/1‑150 (MQ=255)
                                       ggAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCtat                                                                                                                >  2:3654868/1‑150 (MQ=255)
                                        gagaACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCtatt                                                                                                               <  2:1666114/150‑1 (MQ=255)
                                              cGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTg                                                                                                         >  1:2039642/1‑150 (MQ=255)
                                                   gCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTa                                                                                                    <  2:788455/150‑1 (MQ=255)
                                                   gCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTa                                                                                                    <  1:3587285/150‑1 (MQ=255)
                                                   gCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTa                                                                                                    <  2:2998190/150‑1 (MQ=255)
                                                            cGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACTCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtg                                                                                           >  1:2776429/1‑150 (MQ=255)
                                                              cTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtggt                                                                                         <  1:2222972/150‑1 (MQ=255)
                                                              cTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtggt                                                                                         >  1:562386/1‑150 (MQ=255)
                                                              cTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtggt                                                                                         <  1:1496710/150‑1 (MQ=255)
                                                              cTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtggt                                                                                         >  1:3985192/1‑150 (MQ=255)
                                                               tGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAgtggtg                                                                                        <  2:1110539/150‑1 (MQ=255)
                                                                 gACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACTCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTggtggt                                                                                      >  2:2565857/1‑150 (MQ=255)
                                                                 gACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTggtggt                                                                                      <  2:281774/150‑1 (MQ=255)
                                                                  aCTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGtt                                                                                     >  2:1541856/1‑150 (MQ=255)
                                                                   cTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTa                                                                                    >  1:3610143/1‑150 (MQ=255)
                                                                      aCGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCg                                                                                 >  2:1890342/1‑150 (MQ=255)
                                                                        gAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGaa                                                                               >  1:1475767/1‑150 (MQ=255)
                                                                        gAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGaa                                                                               >  1:2101661/1‑150 (MQ=255)
                                                                         aGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAag                                                                              >  2:3981183/1‑150 (MQ=255)
                                                                         aGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAag                                                                              >  2:4477653/1‑150 (MQ=255)
                                                                         aGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAag                                                                              >  2:1777063/1‑150 (MQ=255)
                                                                         aGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAag                                                                              >  1:2783511/1‑150 (MQ=255)
                                                                              aaaGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGaa                                                                         <  1:1294101/150‑1 (MQ=255)
                                                                               aaGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAc                                                                        <  1:2821718/150‑1 (MQ=255)
                                                                               aaGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAc                                                                        <  1:2821854/150‑1 (MQ=255)
                                                                                agagCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAcc                                                                       <  2:3571045/150‑1 (MQ=255)
                                                                                 gagCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccg                                                                      >  2:468420/1‑150 (MQ=255)
                                                                                   gCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACTCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccgcc                                                                    <  1:1925874/150‑1 (MQ=255)
                                                                                   gCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccgcc                                                                    <  2:2055347/150‑1 (MQ=255)
                                                                                   gCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccgcc                                                                    <  2:4303309/150‑1 (MQ=255)
                                                                                   gCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccgcc                                                                    <  2:3429485/150‑1 (MQ=255)
                                                                                   gCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCAGTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAAccgcc                                                                    <  2:3429948/150‑1 (MQ=255)
                                                                                       ctctGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCAt                                                                >  2:4097266/1‑150 (MQ=255)
                                                                                         ctGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACGTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATaa                                                              >  2:963717/1‑150 (MQ=255)
                                                                                          tGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAAc                                                             >  1:3117418/1‑150 (MQ=255)
                                                                                          tGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAAc                                                             <  1:1764769/150‑1 (MQ=255)
                                                                                            ccAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGa                                                           <  2:3740821/150‑1 (MQ=255)
                                                                                             cAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGaa                                                          >  2:2497474/1‑150 (MQ=255)
                                                                                              aaaGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAAt                                                         <  1:4518070/150‑1 (MQ=255)
                                                                                               aaGCCGCTGGAATATCACGCAAAAACTGGTCGATTAGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAAAGGGCTAGTATGGTGAATTAGGTGAAGTGGTGGTAACCGAATAGGAACCGCCGCATAACGAAtt                                                        >  2:29523/1‑150 (MQ=255)
                                                                                                    gCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTAccc                                                   >  2:1935922/1‑150 (MQ=255)
                                                                                                    gCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTAccc                                                   <  2:2227759/150‑1 (MQ=255)
                                                                                                     cTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCa                                                  >  1:2379384/1‑150 (MQ=255)
                                                                                                     cTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCa                                                  >  2:1090734/1‑150 (MQ=255)
                                                                                                         aaTATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATaaa                                              >  1:3346342/1‑150 (MQ=255)
                                                                                                              cACGCAAAAACAGGACGATTTGATTAATGCCAGCGACACGCAACAGTCGGAACTCATTACCTGAAAGGCAACGGGCTATTATGGATACTTAGGAGAATTGGTGGTAACCAAAGAGTAACCGCCGCATAACGAATTTACCCATAAACACTg                                         >  2:2286107/1‑150 (MQ=255)
                                                                                                                cGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTgcg                                       <  2:1149627/150‑1 (MQ=255)
                                                                                                                   aaaaaCTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGcgctc                                    >  2:1335824/1‑150 (MQ=255)
                                                                                                                     aaaCTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGcgctcgc                                  >  1:2751333/1‑150 (MQ=255)
                                                                                                                     aaaCTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGcgctcgc                                  >  2:2530718/1‑150 (MQ=255)
                                                                                                                      aaCTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGcgctcgct                                 >  1:4462498/1‑150 (MQ=255)
                                                                                                                        cTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTgg                               >  2:1305056/1‑150 (MQ=255)
                                                                                                                        cTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTATGCGCTCGCTgg                               >  1:1823554/1‑150 (MQ=255)
                                                                                                                         tGGTCGATTTGATTAATGCCAGCGACACTCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTggg                              >  1:3244395/1‑150 (MQ=255)
                                                                                                                          ggTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGga                             >  1:723260/1‑150 (MQ=255)
                                                                                                                          ggTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGga                             >  1:1209063/1‑150 (MQ=255)
                                                                                                                          ggTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGga                             >  1:3477677/1‑150 (MQ=255)
                                                                                                                              gATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGgaagaa                         >  1:95917/1‑150 (MQ=255)
                                                                                                                              gATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGgaagaa                         <  2:3471962/150‑1 (MQ=255)
                                                                                                                              gATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGgaagaa                         >  1:3826336/1‑150 (MQ=255)
                                                                                                                                     ttAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATtgcct                  <  1:4065383/150‑1 (MQ=255)
                                                                                                                                      tAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATtgcctg                 >  2:3176471/1‑150 (MQ=255)
                                                                                                                                      tAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATtgcctg                 >  2:2481926/1‑150 (MQ=255)
                                                                                                                                      tAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATtgcctg                 >  1:189924/1‑150 (MQ=255)
                                                                                                                                         tGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCg              <  1:4227282/150‑1 (MQ=255)
                                                                                                                                         tGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCg              <  1:4226986/150‑1 (MQ=255)
                                                                                                                                           ccAGCGACACTCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCggg            <  2:392567/150‑1 (MQ=255)
                                                                                                                                             aGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGgcg          <  1:846847/150‑1 (MQ=255)
                                                                                                                                             aGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGgcg          <  1:3041207/150‑1 (MQ=255)
                                                                                                                                               cGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCa        >  1:629269/1‑150 (MQ=255)
                                                                                                                                                gACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCaa       <  2:2961781/150‑1 (MQ=255)
                                                                                                                                                gACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCaa       >  2:3188500/1‑150 (MQ=255)
                                                                                                                                                gACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCaa       <  2:680634/150‑1 (MQ=255)
                                                                                                                                                 acacGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCaaa      >  2:2727278/1‑150 (MQ=255)
                                                                                                                                                   acGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCAAAGt    >  2:554428/1‑150 (MQ=255)
                                                                                                                                                    cGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCAAAGTg   >  2:2569839/1‑150 (MQ=255)
                                                                                                                                                     gCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCAAAGTGa  >  2:3243659/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCAACCTCAACGGCGTTGATGCGGTAATCAACCTGGCCGGAGAACCGATTGCTGATAAACGCTGGACTCACGAGCAAAAAGAGCGTCTCTGCCAAAGCCGCTGGAATATCACGCAAAAACTGGTCGATTTGATTAATGCCAGCGACACGCCACCGTCGGTACTCATTTCCGGCTCGGCAACGGGCTATTATGGTGACTTAGGTGAAGTGGTGGTTACCGAAGAGGAACCGCCGCATAACGAATTTACCCATAAACTCTGCGCTCGCTGGGAAGAAATTGCCTGCCGGGCGCAAAGTGA  >  CP009273/2415349‑2415647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A