Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,416,101 C→A 66.8% intergenic (+21/+27) yfcH → / ← yfcI putative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,416,1010CA66.8% 267.0 / 277.7 241intergenic (+21/+27)yfcH/yfcIputative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base A (76/85);  total (111/130)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 243 total aligned reads displayed.

CTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415952‑2416246
                                                                                                                                                     |                                                                                                                                                 
cTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATcc                                                                                                                                                   <  2:2044534/150‑1 (MQ=255)
    tAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCa                                                                                                                                               <  1:619950/150‑1 (MQ=255)
     aaTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAg                                                                                                                                              >  1:3014663/1‑150 (MQ=255)
       tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGtt                                                                                                                                            <  2:900426/150‑1 (MQ=255)
       tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGtt                                                                                                                                            <  2:4210603/150‑1 (MQ=255)
       tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCAAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGtt                                                                                                                                            <  2:3437531/150‑1 (MQ=255)
          gCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAc                                                                                                                                         <  1:996870/150‑1 (MQ=255)
            gAATCTTCAGTACTGGTATTAGGTAGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTc                                                                                                                                       >  2:4527610/1‑150 (MQ=255)
              aTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTccc                                                                                                                                     <  1:1968185/150‑1 (MQ=255)
              aTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTccc                                                                                                                                     >  2:2975677/1‑150 (MQ=255)
              aTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTccc                                                                                                                                     >  2:1888690/1‑150 (MQ=255)
                 ttCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGgt                                                                                                                                  <  1:822380/150‑1 (MQ=255)
                  tCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGgtg                                                                                                                                 >  2:3617070/1‑150 (MQ=255)
                     gTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTa                                                                                                                              >  1:1764351/1‑150 (MQ=255)
                     gTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTa                                                                                                                              >  2:4093391/1‑150 (MQ=255)
                      tACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTAc                                                                                                                             <  2:2490389/150‑1 (MQ=255)
                        cTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGCTTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCTGTGTTCCAg                                                                                                                           >  2:1117267/1‑150 (MQ=255)
                        cTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAg                                                                                                                           >  2:3716370/1‑150 (MQ=255)
                          ggTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                         <  1:2776772/150‑1 (MQ=255)
                          ggTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                         >  2:238414/1‑150 (MQ=255)
                          ggTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                         <  1:429205/150‑1 (MQ=255)
                          ggTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                         <  1:1582591/150‑1 (MQ=255)
                           gTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGAt                                                                                                                        >  2:1483784/1‑150 (MQ=255)
                            tATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGAtt                                                                                                                       <  1:354169/150‑1 (MQ=255)
                             aTTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTa                                                                                                                      <  1:2100286/150‑1 (MQ=255)
                             aTTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTa                                                                                                                      >  2:861339/1‑150 (MQ=255)
                              ttAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAg                                                                                                                     >  1:422145/1‑150 (MQ=255)
                                aGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTg                                                                                                                   <  2:4933282/150‑1 (MQ=255)
                                    ggACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCtt                                                                                                               >  1:3479634/1‑150 (MQ=255)
                                        aaCGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTgcg                                                                                                           >  1:4660939/1‑150 (MQ=255)
                                          cgcgcgCTGCCTAACCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGcgcg                                                                                                         <  2:4384840/150‑1 (MQ=255)
                                            cgcgcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGAt                                                                                                       <  1:3642274/150‑1 (MQ=255)
                                            cgcgcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGAt                                                                                                       >  2:4731903/1‑150 (MQ=255)
                                             gcgcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATa                                                                                                      <  2:5100009/150‑1 (MQ=255)
                                             gcgcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATa                                                                                                      <  1:4964780/150‑1 (MQ=255)
                                               gcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAg                                                                                                    <  2:3057742/150‑1 (MQ=255)
                                               gcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAg                                                                                                    >  1:4832898/1‑150 (MQ=255)
                                               gcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAg                                                                                                    <  2:2146883/150‑1 (MQ=255)
                                                 tGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATCAGAt                                                                                                  >  1:3934057/1‑150 (MQ=255)
                                                      aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGGTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCt                                                                                             <  2:4636219/150‑1 (MQ=255)
                                                      aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCt                                                                                             >  2:1190394/1‑150 (MQ=255)
                                                         cGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGt                                                                                          <  1:3886775/150‑1 (MQ=255)
                                                          ggCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTg                                                                                         <  2:576190/150‑1 (MQ=255)
                                                            cTGGAAGAAGCGGGTTTTGCGATTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGaa                                                                                       <  2:4882016/150‑1 (MQ=255)
                                                              ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     >  2:1312311/1‑150 (MQ=255)
                                                              ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     <  2:3304896/150‑1 (MQ=255)
                                                               gaagaaGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGt                                                                                    <  2:2283956/150‑1 (MQ=255)
                                                                  gaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCgg                                                                                 <  1:3877360/150‑1 (MQ=255)
                                                                  gaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCgg                                                                                 >  2:2498295/1‑150 (MQ=255)
                                                                   aagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCggg                                                                                <  1:3314708/150‑1 (MQ=255)
                                                                      cGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCa                                                                             <  2:2813419/150‑1 (MQ=255)
                                                                       gggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAc                                                                            >  2:3262308/1‑150 (MQ=255)
                                                                        ggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAcc                                                                           <  2:1695733/150‑1 (MQ=255)
                                                                         gTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACca                                                                          >  2:1749107/1‑150 (MQ=255)
                                                                         gTTTTGCGTGTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACca                                                                          <  2:257739/150‑1 (MQ=255)
                                                                           tttGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatc                                                                        <  1:2086323/150‑1 (MQ=255)
                                                                             tGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatcat                                                                      >  2:307125/1‑150 (MQ=255)
                                                                             tGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatcat                                                                      <  1:1544893/150‑1 (MQ=255)
                                                                                gTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaac                                                                   <  1:3511462/150‑1 (MQ=255)
                                                                                gTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaac                                                                   <  2:4430773/150‑1 (MQ=255)
                                                                                 tttCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaact                                                                  >  1:1006540/1‑150 (MQ=255)
                                                                                 tttCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaact                                                                  >  1:1006410/1‑150 (MQ=255)
                                                                                   tCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaactaa                                                                >  1:327844/1‑150 (MQ=255)
                                                                                     gCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATaactaact                                                              >  1:4088833/1‑150 (MQ=255)
                                                                                      cTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActc                                                             <  1:1941932/150‑1 (MQ=255)
                                                                                      cTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActc                                                             <  1:1942174/150‑1 (MQ=255)
                                                                                       tGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActct                                                            >  2:1359555/1‑150 (MQ=255)
                                                                                       tGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActct                                                            >  1:509811/1‑150 (MQ=255)
                                                                                        ggTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActctc                                                           >  1:1458605/1‑150 (MQ=255)
                                                                                         gTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActctct                                                          <  1:2873727/150‑1 (MQ=255)
                                                                                            cGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTc                                                       <  2:3172171/150‑1 (MQ=255)
                                                                                             gATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCt                                                      <  1:3803693/150‑1 (MQ=255)
                                                                                               tttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTaa                                                    <  2:3638660/150‑1 (MQ=255)
                                                                                                 tAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAc                                                  >  2:2611954/1‑150 (MQ=255)
                                                                                                 tAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAc                                                  <  1:1150551/150‑1 (MQ=255)
                                                                                                                cGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAtct                                   <  1:429961/150‑1 (MQ=255)
                                                                                                                  gATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTcc                                 <  1:1226804/150‑1 (MQ=255)
                                                                                                                  gATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTcc                                 <  1:2681447/150‑1 (MQ=255)
                                                                                                                   aTGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCt                                <  2:2903864/150‑1 (MQ=255)
                                                                                                                    tGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTg                               <  2:1621447/150‑1 (MQ=255)
                                                                                                                      tCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAg                             <  2:3488014/150‑1 (MQ=255)
                                                                                                                       cGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGc                            <  2:3805083/150‑1 (MQ=255)
                                                                                                                       cGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGc                            >  1:132291/1‑150 (MQ=255)
                                                                                                                        gTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCa                           <  1:1377709/150‑1 (MQ=255)
                                                                                                                          tCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAt                         >  2:780302/1‑150 (MQ=255)
                                                                                                                           cGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATa                        >  2:5090952/1‑150 (MQ=255)
                                                                                                                            gCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAc                       <  1:169411/150‑1 (MQ=255)
                                                                                                                              tGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                     <  2:52665/150‑1 (MQ=255)
                                                                                                                               gATGTGGTTGACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTTGTGGCTTTGCGCGATAAGTTCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTATAAACCTCTATCCAGCATCTCCTGAGCAATACGCa                    <  1:4187514/150‑1 (MQ=255)
                                                                                                                                aTGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTCACTCTCTGGCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                   >  2:710805/1‑150 (MQ=255)
                                                                                                                                aTGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                   >  1:3147382/1‑150 (MQ=255)
                                                                                                                                aTGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                   >  1:3922329/1‑150 (MQ=255)
                                                                                                                                 tgtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAgg                  <  1:562270/150‑1 (MQ=255)
                                                                                                                                         aCAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCttcttc          <  2:2056811/150‑1 (MQ=255)
                                                                                                                                         aCAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCttcttc          >  1:441423/1‑150 (MQ=255)
                                                                                                                                            gCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgtg       >  2:4810526/1‑150 (MQ=255)
                                                                                                                                             cAAACATCAGCCCGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgtgt      >  2:1223225/1‑150 (MQ=255)
                                                                                                                                               aaCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGttt    <  1:238414/150‑1 (MQ=255)
                                                                                                                                                aCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTg   >  1:1106847/1‑150 (MQ=255)
                                                                                                                                                 cATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGc  >  1:2582635/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415952‑2416246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTG  >  CP009273/2415956‑2416250
                                                                                                                                                 |                                                                                                                                                     
TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCA                                                                                                                                                   <  SRR19973083.623058/150‑1 (MQ=60)
 AATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAG                                                                                                                                                  >  SRR19973083.3029195/1‑150 (MQ=60)
 AATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAG                                                                                                                                                  <  SRR19973083.1974648/150‑1 (MQ=60)
   TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTT                                                                                                                                                <  SRR19973083.1078967/150‑1 (MQ=60)
    GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTA                                                                                                                                               <  SRR19973083.29449/150‑1 (MQ=60)
    GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTA                                                                                                                                               <  SRR19973083.2335598/150‑1 (MQ=60)
      GCGAATCTTCAGTACTGGTATGAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAC                                                                                                                                             <  SRR19973083.1001855/150‑1 (MQ=60)
        GAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTC                                                                                                                                           <  SRR19973083.3241717/150‑1 (MQ=60)
        GAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTC                                                                                                                                           >  SRR19973083.1183153/1‑150 (MQ=60)
        GAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTC                                                                                                                                           >  SRR19973083.1464647/1‑150 (MQ=60)
          ATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCC                                                                                                                                         <  SRR19973083.1978248/150‑1 (MQ=60)
            CTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGG                                                                                                                                       >  SRR19973083.4549572/1‑150 (MQ=60)
             TTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGT                                                                                                                                      <  SRR19973083.826285/150‑1 (MQ=60)
                 GTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTA                                                                                                                                  >  SRR19973083.3915628/1‑150 (MQ=60)
                 GTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTA                                                                                                                                  >  SRR19973083.1773071/1‑150 (MQ=60)
                   ACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACA                                                                                                                                >  SRR19973083.1679767/1‑150 (MQ=60)
                    CTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAG                                                                                                                               >  SRR19973083.2449022/1‑150 (MQ=60)
                      GGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGA                                                                                                                             <  SRR19973083.2790579/150‑1 (MQ=60)
                      GGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGA                                                                                                                             <  SRR19973083.431114/150‑1 (MQ=60)
                      GGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGA                                                                                                                             <  SRR19973083.1588489/150‑1 (MQ=60)
                      GGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGA                                                                                                                             <  SRR19973083.1590529/150‑1 (MQ=60)
                        TATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATT                                                                                                                           >  SRR19973083.2105577/1‑150 (MQ=60)
                        TATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATT                                                                                                                           <  SRR19973083.355851/150‑1 (MQ=60)
                         ATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTA                                                                                                                          <  SRR19973083.2110924/150‑1 (MQ=60)
                         ATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTA                                                                                                                          <  SRR19973083.1674443/150‑1 (MQ=60)
                          TTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAG                                                                                                                         >  SRR19973083.424032/1‑150 (MQ=60)
                           TAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGAGTAGT                                                                                                                        >  SRR19973083.5799/1‑150 (MQ=60)
                             GGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGG                                                                                                                      >  SRR19973083.4843764/1‑150 (MQ=60)
                              GTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGC                                                                                                                     <  SRR19973083.645181/150‑1 (MQ=60)
                                GGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTT                                                                                                                   >  SRR19973083.3495543/1‑150 (MQ=60)
                                 GACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTT                                                                                                                  <  SRR19973083.4761300/150‑1 (MQ=60)
                                  ACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTG                                                                                                                 <  SRR19973083.3127281/150‑1 (MQ=60)
                                    AACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCG                                                                                                               >  SRR19973083.4680997/1‑150 (MQ=60)
                                       GCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTTGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGA                                                                                                            <  SRR19973083.3832110/150‑1 (MQ=60)
                                       GCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGA                                                                                                            <  SRR19973083.3831766/150‑1 (MQ=60)
                                        CGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGAT                                                                                                           <  SRR19973083.3658936/150‑1 (MQ=60)
                                        CGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGAGTAGTGGCTTTGCGCGAT                                                                                                           >  SRR19973083.762355/1‑150 (MQ=60)
                                         GCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATA                                                                                                          <  SRR19973083.2257003/150‑1 (MQ=60)
                                         GCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATA                                                                                                          <  SRR19973083.4986108/150‑1 (MQ=60)
                                           GCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAG                                                                                                        >  SRR19973083.4853630/1‑150 (MQ=60)
                                            cggcctactCGGCTGGTAGAATCGGGTTTTGCGTTTCGCTGGTACGATTGAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGA                                                                                                       <  SRR19973083.2223660/141‑1 (MQ=60)
                                             TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATCAGAT                                                                                                      >  SRR19973083.3951570/1‑150 (MQ=60)
                                             TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGAGGTTGACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                      >  SRR19973083.3951549/1‑150 (MQ=60)
                                                CTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAATAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTAAAATCCCGGTGTTACAGGATTAGTGGCTTTGAGCGATACGATCGT                                                                                                   >  SRR19973083.3928495/1‑150 (MQ=60)
                                                     CGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGT                                                                                              <  SRR19973083.3904112/150‑1 (MQ=60)
                                                              GAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGG                                                                                     <  SRR19973083.3894787/150‑1 (MQ=60)
                                                              GAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGG                                                                                     <  SRR19973083.3894667/150‑1 (MQ=60)
                                                               AAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGG                                                                                    <  SRR19973083.3330254/150‑1 (MQ=60)
                                                                  CGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCA                                                                                 >  SRR19973083.5098002/1‑150 (MQ=60)
                                                                   GGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAC                                                                                >  SRR19973083.139765/1‑150 (MQ=60)
                                                                    GGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACC                                                                               >  SRR19973083.330847/1‑150 (MQ=60)
                                                                      TTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCAT                                                                             <  SRR19973083.1479955/150‑1 (MQ=60)
                                                                       TTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATC                                                                            <  SRR19973083.2096916/150‑1 (MQ=60)
                                                                         TGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAT                                                                          <  SRR19973083.1552668/150‑1 (MQ=60)
                                                                           CGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGAGAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAA                                                                        >  SRR19973083.923703/1‑150 (MQ=60)
                                                                            GTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAAC                                                                       <  SRR19973083.4193354/150‑1 (MQ=60)
                                                                            GTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAAC                                                                       <  SRR19973083.3527550/150‑1 (MQ=60)
                                                                            GTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAAC                                                                       <  SRR19973083.3527569/150‑1 (MQ=60)
                                                                            GTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGATCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAAC                                                                       >  SRR19973083.494427/1‑150 (MQ=60)
                                                                             TTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACT                                                                      >  SRR19973083.1011448/1‑150 (MQ=60)
                                                                             TTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACT                                                                      >  SRR19973083.1011471/1‑150 (MQ=60)
                                                                             TTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACT                                                                      >  SRR19973083.1011578/1‑150 (MQ=60)
                                                                               TCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAA                                                                    >  SRR19973083.329464/1‑150 (MQ=60)
                                                                                 GCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACT                                                                  >  SRR19973083.4106952/1‑150 (MQ=60)
                                                                                  CTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTC                                                                 <  SRR19973083.1951853/150‑1 (MQ=60)
                                                                                  CTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTC                                                                 <  SRR19973083.1951854/150‑1 (MQ=60)
                                                                                  CTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTC                                                                 <  SRR19973083.1952095/150‑1 (MQ=60)
                                                                                   TGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                                >  SRR19973083.512286/1‑150 (MQ=60)
                                                                                    GGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTC                                                               <  SRR19973083.2797820/150‑1 (MQ=60)
                                                                                    GGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTC                                                               >  SRR19973083.1465956/1‑150 (MQ=60)
                                                                                     GTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCT                                                              <  SRR19973083.2887854/150‑1 (MQ=60)
                                                                                       ACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGT                                                            >  SRR19973083.1993385/1‑150 (MQ=60)
                                                                                         GATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCT                                                          <  SRR19973083.3553247/150‑1 (MQ=60)
                                                                                         GATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCT                                                          <  SRR19973083.3820810/150‑1 (MQ=60)
                                                                                         GATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCT                                                          <  SRR19973083.3553236/150‑1 (MQ=60)
                                                                                          AGTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTA                                                         <  SRR19973083.3409189/150‑1 (MQ=60)
                                                                                             TAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAC                                                      <  SRR19973083.1156290/150‑1 (MQ=60)
                                                                                             TAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAC                                                      <  SRR19973083.1824897/150‑1 (MQ=60)
                                                                                                  GAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTA                                                 >  SRR19973083.5130283/1‑150 (MQ=60)
                                                                                                  GAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTA                                                 <  SRR19973083.4076119/150‑1 (MQ=60)
                                                                                                  GAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTA                                                 >  SRR19973083.2622740/1‑150 (MQ=60)
                                                                                                   AGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTAT                                                >  SRR19973083.4364509/1‑150 (MQ=60)
                                                                                                      CGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCA                                             <  SRR19973083.1343244/150‑1 (MQ=60)
                                                                                                          GGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAT                                         <  SRR19973083.4426877/150‑1 (MQ=60)
                                                                                                            CGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCT                                       >  SRR19973083.4065845/1‑150 (MQ=60)
                                                                                                            CGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCT                                       <  SRR19973083.431871/150‑1 (MQ=60)
                                                                                                            CGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCT                                       <  SRR19973083.431849/150‑1 (MQ=60)
                                                                                                             GGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTC                                      >  SRR19973083.1281129/1‑150 (MQ=60)
                                                                                                              GATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCC                                     <  SRR19973083.2694890/150‑1 (MQ=60)
                                                                                                              GATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCC                                     <  SRR19973083.1232824/150‑1 (MQ=60)
                                                                                                                TGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTG                                   <  SRR19973083.2655407/150‑1 (MQ=60)
                                                                                                                   CGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGC                                >  SRR19973083.133121/1‑150 (MQ=60)
                                                                                                                   CGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGC                                >  SRR19973083.133125/1‑150 (MQ=60)
                                                                                                                    GTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCA                               <  SRR19973083.1384560/150‑1 (MQ=60)
                                                                                                                    GTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCA                               >  SRR19973083.558746/1‑150 (MQ=60)
                                                                                                                        GCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAC                           <  SRR19973083.170389/150‑1 (MQ=60)
                                                                                                                        GCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAC                           <  SRR19973083.170576/150‑1 (MQ=60)
                                                                                                                           GATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCA                        <  SRR19973083.4206103/150‑1 (MQ=60)
                                                                                                                           GATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCA                        >  SRR19973083.4268574/1‑150 (MQ=60)
                                                                                                                           GATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCA                        >  SRR19973083.4268826/1‑150 (MQ=60)
                                                                                                                           GATGTGGTTGACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTTGTGGCTTTGCGCGATAAGTTCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTATAAACCTCTATCCAGCATCTCCTGAGCAATACGCA                        <  SRR19973083.4205942/150‑1 (MQ=60)
                                                                                                                            ATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAG                       >  SRR19973083.3939786/1‑150 (MQ=60)
                                                                                                                            ATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAG                       >  SRR19973083.3162418/1‑150 (MQ=60)
                                                                                                                             TGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGG                      <  SRR19973083.565076/150‑1 (MQ=60)
                                                                                                                             TGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGG                      >  SRR19973083.2786598/1‑150 (MQ=60)
                                                                                                                                  TTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTC                 <  SRR19973083.3580399/150‑1 (MQ=60)
                                                                                                                                    TACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTT               >  SRR19973083.2816987/1‑150 (MQ=60)
                                                                                                                                     ACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTC              <  SRR19973083.3191537/150‑1 (MQ=60)
                                                                                                                                     ACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTC              >  SRR19973083.443386/1‑150 (MQ=60)
                                                                                                                                         CAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGT          <  SRR19973083.1807840/150‑1 (MQ=60)
                                                                                                                                          AAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTT         <  SRR19973083.1955127/150‑1 (MQ=60)
                                                                                                                                           AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT        >  SRR19973083.3732992/1‑150 (MQ=60)
                                                                                                                                           AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT        <  SRR19973083.239698/150‑1 (MQ=60)
                                                                                                                                           AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT        >  SRR19973083.1123711/1‑150 (MQ=60)
                                                                                                                                           AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT        >  SRR19973083.1123472/1‑150 (MQ=60)
                                                                                                                                            ACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTG       <  SRR19973083.3388084/150‑1 (MQ=60)
                                                                                                                                            ACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTG       >  SRR19973083.1112406/1‑150 (MQ=60)
                                                                                                                                             CATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC      >  SRR19973083.2595646/1‑150 (MQ=60)
                                                                                                                                               TCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCC    <  SRR19973083.1366253/150‑1 (MQ=60)
                                                                                                                                               TCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCC    <  SRR19973083.967959/150‑1 (MQ=60)
                                                                                                                                                 AGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTG  >  SRR19973083.2134754/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTG  >  CP009273/2415956‑2416250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑