Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,416,101 C→A 100% intergenic (+21/+27) yfcH → / ← yfcI putative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,416,1010CA100.0% 735.2 / NA 219intergenic (+21/+27)yfcH/yfcIputative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (101/118);  total (101/118)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTG  >  CP009273/2415956‑2416245
                                                                                                                                                 |                                                                                                                                                
  aTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCa                                                                                                                                              >  1:3434635/1‑148 (MQ=255)
  aTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCa                                                                                                                                              >  1:1736265/1‑148 (MQ=255)
   tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAg                                                                                                                                             <  1:2978623/148‑1 (MQ=255)
   tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAg                                                                                                                                             <  1:678790/148‑1 (MQ=255)
    gggCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGt                                                                                                                                            >  2:852110/1‑148 (MQ=255)
     ggCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGtt                                                                                                                                           >  1:2982605/1‑148 (MQ=255)
      gCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTa                                                                                                                                          <  1:1062777/148‑1 (MQ=255)
      gCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTa                                                                                                                                          <  2:75795/148‑1 (MQ=255)
       cGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTaa                                                                                                                                         >  2:505954/1‑148 (MQ=255)
       cGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTaa                                                                                                                                         <  1:1479347/148‑1 (MQ=255)
           tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCTCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCAAGTTAACTcc                                                                                                                                     >  2:3348077/1‑148 (MQ=255)
           tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                     >  2:2965782/1‑148 (MQ=255)
           tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                     <  2:668143/148‑1 (MQ=255)
           tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                     <  2:3423533/148‑1 (MQ=255)
           tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                     <  2:3423866/148‑1 (MQ=255)
               cAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGgt                                                                                                                                 >  1:143786/1‑148 (MQ=255)
                aGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGgtg                                                                                                                                <  1:2590709/148‑1 (MQ=255)
                 gTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGgtgt                                                                                                                               <  2:3486823/148‑1 (MQ=255)
                    cTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTAc                                                                                                                            <  2:398672/148‑1 (MQ=255)
                      ggTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAg                                                                                                                          >  1:460557/1‑148 (MQ=255)
                      ggTATTAGGTGGACAACGCGAGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAg                                                                                                                          >  2:896543/1‑148 (MQ=255)
                       gTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAgg                                                                                                                         >  2:2807606/1‑148 (MQ=255)
                        tATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                        <  1:64039/148‑1 (MQ=255)
                        tATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGa                                                                                                                        >  1:1119211/1‑148 (MQ=255)
                          ttAGGTGGACAACGCGCGCTGCCTAACCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAGCTCCCGGTGTTACAGGAtt                                                                                                                      <  2:2673958/148‑1 (MQ=255)
                           tAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTa                                                                                                                     >  2:2131866/1‑148 (MQ=255)
                             ggTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGt                                                                                                                   >  2:331300/1‑148 (MQ=255)
                                 gACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCt                                                                                                               <  2:117827/148‑1 (MQ=255)
                                     aCGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTgc                                                                                                           >  1:627873/1‑148 (MQ=255)
                                           gcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATa                                                                                                     <  2:3302310/148‑1 (MQ=255)
                                           gcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATa                                                                                                     <  1:891784/148‑1 (MQ=255)
                                             tGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAg                                                                                                   <  2:1474276/148‑1 (MQ=255)
                                              gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGa                                                                                                  >  2:2163656/1‑148 (MQ=255)
                                              gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGa                                                                                                  <  2:1993109/148‑1 (MQ=255)
                                              gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGa                                                                                                  <  2:1558793/148‑1 (MQ=255)
                                                  aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGt                                                                                              <  1:2888080/148‑1 (MQ=255)
                                                  aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGt                                                                                              >  2:2763589/1‑148 (MQ=255)
                                                     cGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTg                                                                                           >  1:3147579/1‑148 (MQ=255)
                                                     cGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTg                                                                                           >  2:2283494/1‑148 (MQ=255)
                                                       gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGt                                                                                         <  2:2527827/148‑1 (MQ=255)
                                                       gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGt                                                                                         <  2:2526733/148‑1 (MQ=255)
                                                       gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGt                                                                                         >  1:3400242/1‑148 (MQ=255)
                                                       gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGt                                                                                         >  1:3464966/1‑148 (MQ=255)
                                                           gaagaaGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGaaa                                                                                     >  2:1612640/1‑148 (MQ=255)
                                                           gaagaaGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGaaa                                                                                     <  1:2538725/148‑1 (MQ=255)
                                                            aagaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                    <  2:2418072/148‑1 (MQ=255)
                                                            aagaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                    >  2:2212561/1‑148 (MQ=255)
                                                              gaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTc                                                                                  >  1:2059374/1‑148 (MQ=255)
                                                              gaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTc                                                                                  >  1:3706675/1‑148 (MQ=255)
                                                               aagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCg                                                                                 <  1:2725243/148‑1 (MQ=255)
                                                                 gCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCggg                                                                               <  1:3138790/148‑1 (MQ=255)
                                                                 gCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCggg                                                                               <  1:1235125/148‑1 (MQ=255)
                                                                  cGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGt                                                                              <  1:3620286/148‑1 (MQ=255)
                                                                  cGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGt                                                                              <  2:543459/148‑1 (MQ=255)
                                                                    ggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCa                                                                            <  1:920040/148‑1 (MQ=255)
                                                                      ttttGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAcc                                                                          <  2:1119211/148‑1 (MQ=255)
                                                                           cGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatcat                                                                     >  1:1641402/1‑148 (MQ=255)
                                                                             tttCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaa                                                                   >  1:3801880/1‑148 (MQ=255)
                                                                             tttCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaa                                                                   <  2:1453163/148‑1 (MQ=255)
                                                                                 gCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaactaa                                                               <  2:1016982/148‑1 (MQ=255)
                                                                                    ggTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActc                                                            <  1:2212561/148‑1 (MQ=255)
                                                                                    ggTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActc                                                            >  2:1976877/1‑148 (MQ=255)
                                                                                          aTTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTc                                                      <  1:1574174/148‑1 (MQ=255)
                                                                                           tttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCt                                                     >  1:1659411/1‑148 (MQ=255)
                                                                                            ttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTa                                                    >  2:3698859/1‑148 (MQ=255)
                                                                                            ttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTa                                                    >  2:311462/1‑148 (MQ=255)
                                                                                               gaagaGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAc                                                 >  2:1628012/1‑148 (MQ=255)
                                                                                                aagaGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAAcc                                                >  1:568117/1‑148 (MQ=255)
                                                                                                  gagGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctc                                              >  2:3203555/1‑148 (MQ=255)
                                                                                                  gagGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctc                                              >  2:1061185/1‑148 (MQ=255)
                                                                                                   agGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGACAGTCGGGTCCCCATCATAACTCACTCTCTGTCTAAACctct                                             >  2:310094/1‑148 (MQ=255)
                                                                                                   agGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctct                                             >  2:3652581/1‑148 (MQ=255)
                                                                                                   agGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctct                                             <  2:2469584/148‑1 (MQ=255)
                                                                                                   agGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctct                                             >  1:1223734/1‑148 (MQ=255)
                                                                                                   agGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACctct                                             <  1:30626/148‑1 (MQ=255)
                                                                                                          ggCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGc                                      <  1:331300/148‑1 (MQ=255)
                                                                                                            cGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAt                                    <  1:613107/148‑1 (MQ=255)
                                                                                                                tGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTcc                                <  2:3400242/148‑1 (MQ=255)
                                                                                                                 gTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCt                               >  1:837128/1‑148 (MQ=255)
                                                                                                                      tCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCa                          <  1:2163656/148‑1 (MQ=255)
                                                                                                                      tCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCa                          <  1:1940692/148‑1 (MQ=255)
                                                                                                                       cGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCaa                         <  1:480694/148‑1 (MQ=255)
                                                                                                                        gCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAt                        <  1:2793983/148‑1 (MQ=255)
                                                                                                                            aTGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                    >  2:3791031/1‑148 (MQ=255)
                                                                                                                              gttgTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                  >  2:2627738/4‑148 (MQ=255)
                                                                                                                              gtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCACTACGCAg                  >  2:1678667/1‑148 (MQ=255)
                                                                                                                              gtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                  >  1:2499143/1‑148 (MQ=255)
                                                                                                                              gtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                  >  1:2533317/1‑148 (MQ=255)
                                                                                                                              gtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAg                  <  2:3060465/148‑1 (MQ=255)
                                                                                                                                 gTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGc               <  2:1736265/148‑1 (MQ=255)
                                                                                                                                  tttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGct              >  1:1199176/1‑148 (MQ=255)
                                                                                                                                   ttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGctt             <  1:2289661/148‑1 (MQ=255)
                                                                                                                                   ttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGctt             <  1:365485/148‑1 (MQ=255)
                                                                                                                                     aCAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGcttct           <  1:186059/148‑1 (MQ=255)
                                                                                                                                     aCAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGcttct           <  1:2119361/148‑1 (MQ=255)
                                                                                                                                        gCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCg        >  1:407257/1‑148 (MQ=255)
                                                                                                                                         cAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgt       >  2:264903/1‑148 (MQ=255)
                                                                                                                                         cAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgt       >  1:2653823/1‑148 (MQ=255)
                                                                                                                                          aaaCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCGGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgtg      <  1:2329134/148‑1 (MQ=255)
                                                                                                                                              aTCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTg  <  2:1920283/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTG  >  CP009273/2415956‑2416245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCT  >  CP009273/2415952‑2416249
                                                                                                                                                     |                                                                                                                                                    
CTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCA                                                                                                                                                      >  SRR19973069.2569763/1‑150 (MQ=60)
  GTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGC                                                                                                                                                    >  SRR19973069.3080708/1‑150 (MQ=60)
    TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCA                                                                                                                                                  >  SRR19973069.1748438/1‑150 (MQ=60)
    TAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCA                                                                                                                                                  >  SRR19973069.3456953/1‑150 (MQ=60)
       TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                               <  SRR19973069.2998410/150‑1 (MQ=60)
       TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                               >  SRR19973069.3002406/1‑150 (MQ=60)
       TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                               <  SRR19973069.683505/150‑1 (MQ=60)
       TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                               <  SRR19973069.2998398/150‑1 (MQ=60)
       TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                               <  SRR19973069.2998244/150‑1 (MQ=60)
          GCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAC                                                                                                                                            <  SRR19973069.3466690/150‑1 (MQ=60)
          GCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAC                                                                                                                                            <  SRR19973069.1070182/150‑1 (MQ=60)
           CGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACT                                                                                                                                           <  SRR19973069.1489639/150‑1 (MQ=60)
           CGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACT                                                                                                                                           <  SRR19973069.3401617/150‑1 (MQ=60)
                 TTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGT                                                                                                                                     >  SRR19973069.147426/1‑150 (MQ=60)
                 TTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGT                                                                                                                                     >  SRR19973069.145084/1‑150 (MQ=60)
                    AGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTT                                                                                                                                  <  SRR19973069.2607979/150‑1 (MQ=60)
                    AGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTT                                                                                                                                  <  SRR19973069.2210243/150‑1 (MQ=60)
                    AGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTT                                                                                                                                  <  SRR19973069.3177645/150‑1 (MQ=60)
                        CTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAG                                                                                                                              >  SRR19973069.463934/1‑150 (MQ=60)
                        CTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAG                                                                                                                              >  SRR19973069.2210313/1‑150 (MQ=60)
                          GGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGA                                                                                                                            >  SRR19973069.1127015/1‑150 (MQ=60)
                            TATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATT                                                                                                                          <  SRR19973069.64791/150‑1 (MQ=60)
                             ATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTA                                                                                                                         >  SRR19973069.1237152/1‑150 (MQ=60)
                                AGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTG                                                                                                                      <  SRR19973069.3587046/150‑1 (MQ=60)
                                AGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTG                                                                                                                      <  SRR19973069.2324794/150‑1 (MQ=60)
                                    GGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCCAACATCAGCCAGTTAACTCACGGTGTTACAGGATTAGTGGCTT                                                                                                                  >  SRR19973069.466655/1‑150 (MQ=60)
                                    GGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTT                                                                                                                  <  SRR19973069.1120222/150‑1 (MQ=60)
                                     GACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTT                                                                                                                 <  SRR19973069.3794745/150‑1 (MQ=60)
                                      ACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTG                                                                                                                <  SRR19973069.2682980/150‑1 (MQ=60)
                                      ACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTG                                                                                                                <  SRR19973069.664831/150‑1 (MQ=60)
                                       CAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGC                                                                                                               >  SRR19973069.632033/1‑150 (MQ=60)
                                       CAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGC                                                                                                               >  SRR19973069.2278709/1‑150 (MQ=60)
                                               GCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAG                                                                                                       <  SRR19973069.3429195/150‑1 (MQ=60)
                                               GCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAG                                                                                                       <  SRR19973069.1299781/150‑1 (MQ=60)
                                               GCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAG                                                                                                       <  SRR19973069.897932/150‑1 (MQ=60)
                                                      AAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCT                                                                                                <  SRR19973069.370949/150‑1 (MQ=60)
                                                      AAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCT                                                                                                <  SRR19973069.2907100/150‑1 (MQ=60)
                                                       AACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTG                                                                                               >  SRR19973069.3168159/1‑150 (MQ=60)
                                                       AACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTG                                                                                               >  SRR19973069.1648364/1‑150 (MQ=60)
                                                       AACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTG                                                                                               >  SRR19973069.3168162/1‑150 (MQ=60)
                                                         CGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGT                                                                                             <  SRR19973069.896496/150‑1 (MQ=60)
                                                         CGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGT                                                                                             >  SRR19973069.3422423/1‑150 (MQ=60)
                                                         CGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGT                                                                                             >  SRR19973069.3487390/1‑150 (MQ=60)
                                                              GGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAG                                                                                        <  SRR19973069.3674954/150‑1 (MQ=60)
                                                               GAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATTTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGT                                                                                       <  SRR19973069.2555552/150‑1 (MQ=60)
                                                               GAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGT                                                                                       <  SRR19973069.2555536/150‑1 (MQ=60)
                                                                AAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTC                                                                                      >  SRR19973069.1198762/1‑150 (MQ=60)
                                                                AAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTC                                                                                      >  SRR19973069.2073657/1‑150 (MQ=60)
                                                                AAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTC                                                                                      >  SRR19973069.3730893/1‑150 (MQ=60)
                                                                 AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                     >  SRR19973069.607968/1‑150 (MQ=60)
                                                                 AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                     <  SRR19973069.1731499/150‑1 (MQ=60)
                                                                 AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                     <  SRR19973069.1489849/150‑1 (MQ=60)
                                                                   AAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGG                                                                                   <  SRR19973069.815953/150‑1 (MQ=60)
                                                                   AAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGG                                                                                   <  SRR19973069.2743498/150‑1 (MQ=60)
                                                                     GCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTC                                                                                 <  SRR19973069.3159348/150‑1 (MQ=60)
                                                                     GCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTC                                                                                 <  SRR19973069.1243537/150‑1 (MQ=60)
                                                                      CGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCA                                                                                <  SRR19973069.3643939/150‑1 (MQ=60)
                                                                        GGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACC                                                                              <  SRR19973069.926311/150‑1 (MQ=60)
                                                                        GGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACC                                                                              <  SRR19973069.926334/150‑1 (MQ=60)
                                                                          TTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCAT                                                                            <  SRR19973069.39602/150‑1 (MQ=60)
                                                                             TGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAT                                                                         >  SRR19973069.1652679/1‑150 (MQ=60)
                                                                             TGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAT                                                                         >  SRR19973069.1652738/1‑150 (MQ=60)
                                                                               CGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAA                                                                       >  SRR19973069.3826505/1‑150 (MQ=60)
                                                                                GTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCACTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAAC                                                                      >  SRR19973069.2662183/1‑150 (MQ=60)
                                                                                   TCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAA                                                                   >  SRR19973069.3668844/1‑150 (MQ=60)
                                                                                        GGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTC                                                              >  SRR19973069.1461849/1‑150 (MQ=60)
                                                                                        GGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTC                                                              <  SRR19973069.2227549/150‑1 (MQ=60)
                                                                                        GGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTC                                                              >  SRR19973069.2127537/1‑150 (MQ=60)
                                                                                         GTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCT                                                             >  SRR19973069.914245/1‑150 (MQ=60)
                                                                                           ACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGT                                                           <  SRR19973069.3261671/150‑1 (MQ=60)
                                                                                             GATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCT                                                         >  SRR19973069.1670903/1‑150 (MQ=60)
                                                                                              ATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTA                                                        <  SRR19973069.1584876/150‑1 (MQ=60)
                                                                                               TTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAA                                                       <  SRR19973069.2567332/150‑1 (MQ=60)
                                                                                                  AGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACC                                                    >  SRR19973069.572059/1‑150 (MQ=60)
                                                                                                    AAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTC                                                  >  SRR19973069.314012/1‑150 (MQ=60)
                                                                                                     AGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCT                                                 >  SRR19973069.1232098/1‑150 (MQ=60)
                                                                                                     AGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCT                                                 >  SRR19973069.2160929/1‑150 (MQ=60)
                                                                                                       AGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTAT                                               <  SRR19973069.31058/150‑1 (MQ=60)
                                                                                                        GGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATC                                              <  SRR19973069.527572/150‑1 (MQ=60)
                                                                                                              GGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAT                                        <  SRR19973069.333571/150‑1 (MQ=60)
                                                                                                                CGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCT                                      <  SRR19973069.617205/150‑1 (MQ=60)
                                                                                                                CGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCT                                      <  SRR19973069.617353/150‑1 (MQ=60)
                                                                                                                 GGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTC                                     <  SRR19973069.1866125/150‑1 (MQ=60)
                                                                                                                  GATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCC                                    <  SRR19973069.509568/150‑1 (MQ=60)
                                                                                                                   ATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCT                                   >  SRR19973069.843013/1‑150 (MQ=60)
                                                                                                                    TGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTG                                  <  SRR19973069.2826221/150‑1 (MQ=60)
                                                                                                                          TCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAT                            <  SRR19973069.1954051/150‑1 (MQ=60)
                                                                                                                          TCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAT                            <  SRR19973069.2178452/150‑1 (MQ=60)
                                                                                                                           CGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATA                           <  SRR19973069.484181/150‑1 (MQ=60)
                                                                                                                            GCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAC                          <  SRR19973069.2812496/150‑1 (MQ=60)
                                                                                                                            GCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAC                          <  SRR19973069.2812555/150‑1 (MQ=60)
                                                                                                                                ATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAG                      >  SRR19973069.2552240/1‑150 (MQ=60)
                                                                                                                                ATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAG                      >  SRR19973069.2515838/1‑150 (MQ=60)
                                                                                                                                ATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAG                      >  SRR19973069.2550108/1‑150 (MQ=60)
                                                                                                                                    GGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCT                  >  SRR19973069.1207677/1‑150 (MQ=60)
                                                                                                                                    GGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCT                  >  SRR19973069.1207421/1‑150 (MQ=60)
                                                                                                                                       TTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCT               <  SRR19973069.2305220/150‑1 (MQ=60)
                                                                                                                                       TTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCT               <  SRR19973069.368128/150‑1 (MQ=60)
                                                                                                                                        TACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTT              >  SRR19973069.776241/1‑150 (MQ=60)
                                                                                                                                         ACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTC             <  SRR19973069.187596/150‑1 (MQ=60)
                                                                                                                                         ACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTC             <  SRR19973069.1910620/150‑1 (MQ=60)
                                                                                                                                         ACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTC             <  SRR19973069.2133964/150‑1 (MQ=60)
                                                                                                                                          CAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCG            >  SRR19973069.410255/1‑150 (MQ=60)
                                                                                                                                          CAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCG            >  SRR19973069.410237/1‑150 (MQ=60)
                                                                                                                                           AGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGT           >  SRR19973069.64416/1‑150 (MQ=60)
                                                                                                                                           AGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGT           >  SRR19973069.2671607/1‑150 (MQ=60)
                                                                                                                                             CAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGT         >  SRR19973069.2516995/1‑150 (MQ=60)
                                                                                                                                             CAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGT         >  SRR19973069.2515359/1‑150 (MQ=60)
                                                                                                                                              AAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTT        <  SRR19973069.2977352/150‑1 (MQ=60)
                                                                                                                                              AAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCGGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTT        <  SRR19973069.2344980/150‑1 (MQ=60)
                                                                                                                                               AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT       >  SRR19973069.1962809/1‑150 (MQ=60)
                                                                                                                                               AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT       >  SRR19973069.1762927/1‑150 (MQ=60)
                                                                                                                                                   TCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCC   <  SRR19973069.3350859/150‑1 (MQ=60)
                                                                                                                                                    CAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCT  <  SRR19973069.2985469/150‑1 (MQ=60)
                                                                                                                                                    CAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCT  <  SRR19973069.947427/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTGTTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCT  >  CP009273/2415952‑2416249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑