Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,416,101 C→A 100% intergenic (+21/+27) yfcH → / ← yfcI putative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,416,1010CA98.8% 736.8 / ‑0.9 212intergenic (+21/+27)yfcH/yfcIputative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (104/104);  total (106/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415955‑2416246
                                                                                                                                                  |                                                                                                                                                 
 tAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCa                                                                                                                                               <  2:3837618/150‑1 (MQ=255)
  aaTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAg                                                                                                                                              >  1:1374943/1‑150 (MQ=255)
  aaTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAg                                                                                                                                              >  2:1448833/1‑150 (MQ=255)
  aaTGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAg                                                                                                                                              <  2:2767777/150‑1 (MQ=255)
    tGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGtt                                                                                                                                            <  1:1101947/150‑1 (MQ=255)
     gggCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTa                                                                                                                                           <  1:489107/150‑1 (MQ=255)
      ggCGAATCTTCAGTACAGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTAAGAAGAGGCGCTGGCGGATGTCGTTCTCTGATGTGGTTTACACCAAACATCAGCCAGTTaa                                                                                                                                          >  1:1328305/1‑150 (MQ=255)
       gCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAc                                                                                                                                         >  1:3957090/1‑150 (MQ=255)
          aaTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                      >  2:229438/1‑150 (MQ=255)
          aaTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                      >  2:1355662/1‑150 (MQ=255)
          aaTCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTcc                                                                                                                                      >  1:4086762/1‑150 (MQ=255)
            tCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCg                                                                                                                                    <  2:301218/150‑1 (MQ=255)
                 aGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGtt                                                                                                                               >  1:3870205/1‑150 (MQ=255)
                   tACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTAc                                                                                                                             >  2:3454250/1‑150 (MQ=255)
                   tACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTAc                                                                                                                             <  2:1359922/150‑1 (MQ=255)
                         gaTTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTGTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGAtt                                                                                                                       >  2:1269437/2‑150 (MQ=255)
                          aTTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTa                                                                                                                      <  1:680291/150‑1 (MQ=255)
                            tAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGt                                                                                                                    >  1:776293/1‑150 (MQ=255)
                              ggTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTgg                                                                                                                  <  2:2542542/150‑1 (MQ=255)
                              ggTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTgg                                                                                                                  <  1:820640/150‑1 (MQ=255)
                              ggTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTgg                                                                                                                  <  2:460863/150‑1 (MQ=255)
                                  gACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCttt                                                                                                              <  1:2918660/150‑1 (MQ=255)
                                   aCAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTg                                                                                                             <  2:1622828/150‑1 (MQ=255)
                                     aaCGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTgcg                                                                                                           >  1:2550344/1‑150 (MQ=255)
                                     aaCGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTgcg                                                                                                           >  1:178040/1‑150 (MQ=255)
                                      aCGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTgcgc                                                                                                          >  2:4085776/1‑150 (MQ=255)
                                          gcgcTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATa                                                                                                      >  2:3670646/1‑150 (MQ=255)
                                              tGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAt                                                                                                  >  1:264808/1‑150 (MQ=255)
                                              tGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAt                                                                                                  >  1:264773/1‑150 (MQ=255)
                                              tGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTAAGAAGAGGCGCTGGCGGATGTCGTTCTCTGATGTTGTTTACAGCAAACATCCTCCAGTTAACTCCCGGAGTTACAGGATTAGTGGCTTTGCGCGATAAGAt                                                                                                  >  1:3438031/1‑150 (MQ=255)
                                               gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATc                                                                                                 <  1:4153617/150‑1 (MQ=255)
                                               gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATc                                                                                                 >  1:3197442/1‑150 (MQ=255)
                                               gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATc                                                                                                 <  2:3722625/150‑1 (MQ=255)
                                               gCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATc                                                                                                 <  2:1212826/150‑1 (MQ=255)
                                                ccTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCg                                                                                                >  2:1735331/1‑150 (MQ=255)
                                                 cTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGt                                                                                               >  2:2687680/1‑150 (MQ=255)
                                                  tAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTc                                                                                              <  2:1314174/150‑1 (MQ=255)
                                                   aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCt                                                                                             >  1:1871659/1‑150 (MQ=255)
                                                   aaaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCt                                                                                             >  1:2608410/1‑150 (MQ=255)
                                                    aaCGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTg                                                                                            >  1:18064/1‑150 (MQ=255)
                                                        gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGa                                                                                        >  1:1976143/1‑150 (MQ=255)
                                                        gCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGa                                                                                        <  2:1177691/150‑1 (MQ=255)
                                                           ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     <  1:3119334/150‑1 (MQ=255)
                                                           ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     <  1:794384/150‑1 (MQ=255)
                                                           ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     <  2:1107169/150‑1 (MQ=255)
                                                           ggAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAg                                                                                     <  2:1107486/150‑1 (MQ=255)
                                                             aagaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTc                                                                                   <  2:285428/150‑1 (MQ=255)
                                                             aagaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTc                                                                                   <  1:2119491/150‑1 (MQ=255)
                                                              agaagCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCg                                                                                  <  2:654636/150‑1 (MQ=255)
                                                                 agCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGt                                                                               >  1:2719876/1‑150 (MQ=255)
                                                                 agCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGt                                                                               >  1:1396618/1‑150 (MQ=255)
                                                                  gCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGAGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCTGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTc                                                                              <  1:2056604/150‑1 (MQ=255)
                                                                    gggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAc                                                                            >  2:2596399/1‑150 (MQ=255)
                                                                    gggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAc                                                                            >  1:1397111/1‑150 (MQ=255)
                                                                     ggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAcc                                                                           <  2:3998838/150‑1 (MQ=255)
                                                                     ggTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAcc                                                                           >  2:1147495/1‑150 (MQ=255)
                                                                      gTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACca                                                                          <  2:1442381/150‑1 (MQ=255)
                                                                        tttGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatc                                                                        <  1:2372560/150‑1 (MQ=255)
                                                                          tGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACcatcat                                                                      <  1:2742281/150‑1 (MQ=255)
                                                                            cGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaa                                                                    <  1:490954/150‑1 (MQ=255)
                                                                            cGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAtaa                                                                    <  2:888423/150‑1 (MQ=255)
                                                                                  gCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATaactaact                                                              <  2:2901072/150‑1 (MQ=255)
                                                                                    tGGTACGATTTAGTAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActct                                                            <  1:3846435/150‑1 (MQ=255)
                                                                                    tGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActct                                                            >  2:4163009/1‑150 (MQ=255)
                                                                                     ggTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActctc                                                           <  2:3161450/150‑1 (MQ=255)
                                                                                      gTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAActctct                                                          >  2:2139562/1‑150 (MQ=255)
                                                                                            tttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTaa                                                    >  1:4030713/1‑150 (MQ=255)
                                                                                             ttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTaaa                                                   >  1:4105866/1‑150 (MQ=255)
                                                                                             ttAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTaaa                                                   >  2:1946162/1‑150 (MQ=255)
                                                                                                     ggCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATc                                           >  2:1904257/1‑150 (MQ=255)
                                                                                                     ggCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATc                                           >  2:1904158/1‑150 (MQ=255)
                                                                                                       cgcTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCa                                         >  1:2157851/1‑150 (MQ=255)
                                                                                                        gcTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAg                                        <  2:954562/150‑1 (MQ=255)
                                                                                                         cTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGc                                       <  2:2260597/150‑1 (MQ=255)
                                                                                                           ggCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAt                                     <  2:3601464/150‑1 (MQ=255)
                                                                                                           ggCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAt                                     >  1:2975656/1‑150 (MQ=255)
                                                                                                            gCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAtc                                    <  1:3162002/150‑1 (MQ=255)
                                                                                                              ggATGTCGNTCGCTGANGTGGTTTACAGCANACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACNATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAtctc                                  >  2:3500634/1‑150 (MQ=255)
                                                                                                               gATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTcc                                 <  2:113693/150‑1 (MQ=255)
                                                                                                                 tGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTg                               >  1:1667229/1‑150 (MQ=255)
                                                                                                                  gTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGa                              <  1:128669/150‑1 (MQ=255)
                                                                                                                     gTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCa                           >  1:1199535/1‑150 (MQ=255)
                                                                                                                     gTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCa                           >  1:1960719/1‑150 (MQ=255)
                                                                                                                      ttCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCaa                          <  2:2666151/150‑1 (MQ=255)
                                                                                                                       tCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAt                         >  1:3210660/1‑150 (MQ=255)
                                                                                                                          cTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACg                      >  1:3600902/1‑150 (MQ=255)
                                                                                                                           tGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                     >  2:1683407/1‑150 (MQ=255)
                                                                                                                           tGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                     >  1:995300/1‑150 (MQ=255)
                                                                                                                           tGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                     >  1:795595/1‑150 (MQ=255)
                                                                                                                           tGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGc                     >  2:3728963/1‑150 (MQ=255)
                                                                                                                               gtgGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAggg                 <  1:1499513/150‑1 (MQ=255)
                                                                                                                                 ggTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGct               >  2:1994028/1‑150 (MQ=255)
                                                                                                                                 gctttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGct               >  1:3775283/3‑150 (MQ=255)
                                                                                                                                  gTTTACAGCAACCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGctt              <  1:3048097/150‑1 (MQ=255)
                                                                                                                                   tttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGcttc             <  2:4155230/150‑1 (MQ=255)
                                                                                                                                   tttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGcttc             <  2:3538054/150‑1 (MQ=255)
                                                                                                                                   tttACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGcttc             <  2:179052/150‑1 (MQ=255)
                                                                                                                                          cAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCgtgt      >  1:2882678/1‑150 (MQ=255)
                                                                                                                                              cATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGc  >  1:3297832/1‑150 (MQ=255)
                                                                                                                                              cATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGc  <  2:1642753/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTAATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415955‑2416246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415957‑2416246
                                                                                                                                                |                                                                                                                                                 
AATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAG                                                                                                                                              >  SRR19973074.1377485/1‑150 (MQ=60)
 ATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGT                                                                                                                                             <  SRR19973074.805320/150‑1 (MQ=60)
  TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                            <  SRR19973074.1104408/150‑1 (MQ=60)
  TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                            >  SRR19973074.2544171/1‑150 (MQ=60)
  TGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTT                                                                                                                                            <  SRR19973074.1104010/150‑1 (MQ=60)
   GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTA                                                                                                                                           <  SRR19973074.1940223/150‑1 (MQ=60)
   GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTA                                                                                                                                           >  SRR19973074.3486757/1‑150 (MQ=60)
   GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTA                                                                                                                                           <  SRR19973074.449492/150‑1 (MQ=60)
   GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTA                                                                                                                                           <  SRR19973074.490089/150‑1 (MQ=60)
   GGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTA                                                                                                                                           <  SRR19973074.490133/150‑1 (MQ=60)
    GGCGAATCTTCAGTACAGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTAAGAAGAGGCGCTGGCGGATGTCGTTCTCTGATGTGGTTTACACCAAACATCAGCCAGTTAA                                                                                                                                          >  SRR19973074.1330793/1‑150 (MQ=60)
     GCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAC                                                                                                                                         >  SRR19973074.3964384/1‑150 (MQ=60)
     GCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAAC                                                                                                                                         >  SRR19973074.3964383/1‑150 (MQ=60)
        AATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCC                                                                                                                                      >  SRR19973074.4094322/1‑150 (MQ=60)
          TCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCG                                                                                                                                    <  SRR19973074.2134917/150‑1 (MQ=60)
               AGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTT                                                                                                                               >  SRR19973074.3877283/1‑150 (MQ=60)
                  ACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCACTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACA                                                                                                                            <  SRR19973074.1373522/150‑1 (MQ=60)
                        ATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTA                                                                                                                      <  SRR19973074.681543/150‑1 (MQ=60)
                          TAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGT                                                                                                                    >  SRR19973074.777779/1‑150 (MQ=60)
                          TAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGT                                                                                                                    >  SRR19973074.777935/1‑150 (MQ=60)
                           AGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTG                                                                                                                   <  SRR19973074.130395/150‑1 (MQ=60)
                            GGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGG                                                                                                                  <  SRR19973074.822216/150‑1 (MQ=60)
                                GACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTT                                                                                                              <  SRR19973074.2924084/150‑1 (MQ=60)
                                GACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTT                                                                                                              <  SRR19973074.167182/150‑1 (MQ=60)
                                 ACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTG                                                                                                             >  SRR19973074.1712919/1‑150 (MQ=60)
                                   AACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCG                                                                                                           >  SRR19973074.2555105/1‑150 (MQ=60)
                                   AACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCG                                                                                                           >  SRR19973074.178423/1‑150 (MQ=60)
                                            TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                  >  SRR19973074.3271920/1‑150 (MQ=60)
                                            TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                  >  SRR19973074.265328/1‑150 (MQ=60)
                                            TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                  >  SRR19973074.265299/1‑150 (MQ=60)
                                            TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                  >  SRR19973074.265293/1‑150 (MQ=60)
                                            TGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTAAGAAGAGGCGCTGGCGGATGTCGTTCTCTGATGTTGTTTACAGCAAACATCCTCCAGTTAACTCCCGGAGTTACAGGATTAGTGGCTTTGCGCGATAAGAT                                                                                                  >  SRR19973074.3444363/1‑150 (MQ=60)
                                             GCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATC                                                                                                 <  SRR19973074.4161265/150‑1 (MQ=60)
                                             GCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATC                                                                                                 >  SRR19973074.3203316/1‑150 (MQ=60)
                                             GCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATC                                                                                                 >  SRR19973074.3203336/1‑150 (MQ=60)
                                                 AAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCT                                                                                             >  SRR19973074.2613292/1‑150 (MQ=60)
                                                 AAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCT                                                                                             >  SRR19973074.1875244/1‑150 (MQ=60)
                                                  AACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTG                                                                                            >  SRR19973074.18116/1‑150 (MQ=60)
                                                    CGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGT                                                                                          <  SRR19973074.3808008/150‑1 (MQ=60)
                                                      GCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGA                                                                                        >  SRR19973074.1979905/1‑150 (MQ=60)
                                                      GCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGCGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGA                                                                                        <  SRR19973074.995071/150‑1 (MQ=60)
                                                        TGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAATAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAA                                                                                      >  SRR19973074.659611/1‑150 (MQ=60)
                                                        TGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGATGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGTATTAGTGGCTTTGCGCGATAATATCGTCTGGTGAAA                                                                                      >  SRR19973074.2256759/1‑150 (MQ=60)
                                                        TGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAA                                                                                      >  SRR19973074.3061885/1‑150 (MQ=60)
                                                         GGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAACCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAG                                                                                     <  SRR19973074.795926/150‑1 (MQ=60)
                                                         GGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAG                                                                                     <  SRR19973074.4116725/150‑1 (MQ=60)
                                                         GGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAG                                                                                     <  SRR19973074.3125054/150‑1 (MQ=60)
                                                         GGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAG                                                                                     <  SRR19973074.795909/150‑1 (MQ=60)
                                                           AAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTATTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTC                                                                                   <  SRR19973074.3929809/150‑1 (MQ=60)
                                                           AAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTC                                                                                   <  SRR19973074.2123471/150‑1 (MQ=60)
                                                            AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                  <  SRR19973074.3213838/150‑1 (MQ=60)
                                                            AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                  >  SRR19973074.336764/1‑150 (MQ=60)
                                                            AGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCG                                                                                  <  SRR19973074.752144/150‑1 (MQ=60)
                                                               AGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGT                                                                               >  SRR19973074.2724905/1‑150 (MQ=60)
                                                               AGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGT                                                                               >  SRR19973074.1399183/1‑150 (MQ=60)
                                                                GCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGAGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCTGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTC                                                                              <  SRR19973074.2060468/150‑1 (MQ=60)
                                                                  GGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAC                                                                            >  SRR19973074.3050822/1‑150 (MQ=60)
                                                                  GGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCAC                                                                            >  SRR19973074.1399676/1‑150 (MQ=60)
                                                                     TTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCAT                                                                         <  SRR19973074.3160835/150‑1 (MQ=60)
                                                                     TTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCAT                                                                         >  SRR19973074.4060676/1‑150 (MQ=60)
                                                                      TTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATC                                                                        <  SRR19973074.2376973/150‑1 (MQ=60)
                                                                      TTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATC                                                                        <  SRR19973074.1382269/150‑1 (MQ=60)
                                                                        TGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAT                                                                      <  SRR19973074.2747336/150‑1 (MQ=60)
                                                                        TGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCAT                                                                      <  SRR19973074.2746875/150‑1 (MQ=60)
                                                                          CGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAA                                                                    <  SRR19973074.2578365/150‑1 (MQ=60)
                                                                          CGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAA                                                                    <  SRR19973074.491939/150‑1 (MQ=60)
                                                                                  TGGTACGATTTAGTAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                            <  SRR19973074.3853451/150‑1 (MQ=60)
                                                                                  TGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                            >  SRR19973074.3655016/1‑150 (MQ=60)
                                                                                  TGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                            >  SRR19973074.2890452/1‑150 (MQ=60)
                                                                                  TGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                            <  SRR19973074.3853509/150‑1 (MQ=60)
                                                                                  TGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCT                                                            >  SRR19973074.2890137/1‑150 (MQ=60)
                                                                                        GATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCT                                                      >  SRR19973074.2113729/1‑150 (MQ=60)
                                                                                         ATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTA                                                     >  SRR19973074.1639750/1‑150 (MQ=60)
                                                                                          TTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAA                                                    >  SRR19973074.4038165/1‑150 (MQ=60)
                                                                                          TTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAA                                                    >  SRR19973074.2845041/1‑150 (MQ=60)
                                                                                           TTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAA                                                   >  SRR19973074.4113440/1‑150 (MQ=60)
                                                                                             AGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACC                                                 <  SRR19973074.1807381/150‑1 (MQ=60)
                                                                                             AGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACC                                                 >  SRR19973074.4145375/1‑150 (MQ=60)
                                                                                             AGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACC                                                 >  SRR19973074.908011/1‑150 (MQ=60)
                                                                                                 GAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTA                                             >  SRR19973074.4107515/1‑150 (MQ=60)
                                                                                                   GGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATC                                           <  SRR19973074.1832263/150‑1 (MQ=60)
                                                                                                    GCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCC                                          <  SRR19973074.2369377/150‑1 (MQ=60)
                                                                                                     CGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCA                                         >  SRR19973074.2161910/1‑150 (MQ=60)
                                                                                                       CTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGC                                       >  SRR19973074.1399621/1‑150 (MQ=60)
                                                                                                         GGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAT                                     >  SRR19973074.2981180/1‑150 (MQ=60)
                                                                                                         GGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCAT                                     >  SRR19973074.2981160/1‑150 (MQ=60)
                                                                                                          GCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATC                                    <  SRR19973074.3167823/150‑1 (MQ=60)
                                                                                                            GGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTC                                  >  SRR19973074.2681080/1‑150 (MQ=60)
                                                                                                               TGTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTG                               >  SRR19973074.1670372/1‑150 (MQ=60)
                                                                                                                GTCGTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGA                              <  SRR19973074.128964/150‑1 (MQ=60)
                                                                                                                   GTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCA                           >  SRR19973074.1201830/1‑150 (MQ=60)
                                                                                                                   GTTCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCA                           >  SRR19973074.1964463/1‑150 (MQ=60)
                                                                                                                     TCGCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAAT                         >  SRR19973074.3216585/1‑150 (MQ=60)
                                                                                                                       GCTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATAC                       >  SRR19973074.1431006/1‑150 (MQ=60)
                                                                                                                        CTGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACG                      >  SRR19973074.3607555/1‑150 (MQ=60)
                                                                                                                         TGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGC                     >  SRR19973074.797125/1‑150 (MQ=60)
                                                                                                                         TGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGC                     <  SRR19973074.19699/150‑1 (MQ=60)
                                                                                                                         TGATGTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGC                     >  SRR19973074.997156/1‑150 (MQ=60)
                                                                                                                             GTGGTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGG                 <  SRR19973074.1502347/150‑1 (MQ=60)
                                                                                                                               GCTTTACAGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCT               >  SRR19973074.3782211/1‑150 (MQ=60)
                                                                                                                                GTTTACAGCAACCATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTT              <  SRR19973074.3053727/150‑1 (MQ=60)
                                                                                                                                      AGCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGT        <  SRR19973074.120875/150‑1 (MQ=60)
                                                                                                                                       GCAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTG       <  SRR19973074.3460603/150‑1 (MQ=60)
                                                                                                                                        CAAACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGT      >  SRR19973074.2888027/1‑150 (MQ=60)
                                                                                                                                          AACATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTT    >  SRR19973074.2648640/1‑150 (MQ=60)
                                                                                                                                            CATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  SRR19973074.3303948/1‑150 (MQ=60)
                                                                                                                                            CATCAGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  SRR19973074.2010163/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                 
AATGGGCGAATCTTCAGTACTGGTATTAGGTGGACAACGCGCGCTGCCTAAACGGCTGGAAGAAGCGGGTTTTGCGTTTCGCTGGTACGATTTAGAAGAGGCGCTGGCGGATGTCGTTCGCTGATGTGGTTTACAGCAAACATCCGCCAGTTAACTCCCGGTGTTACAGGATTAGTGGCTTTGCGCGATAAGATCGTCTGGTGAAAGTCGGGTCACCATCATAACTAACTCTCTGTCTAAACCTCTATCCAGCATCTCCTGAGCAATACGCAGGGCTTCTTCGTGTTTGC  >  CP009273/2415957‑2416246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑