Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,522,624 C→A 15.9% A304A (GCG→GCT)  ligA ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,522,6240CA15.9% 918.7 / 102.8 281A304A (GCG→GCT) ligADNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (108/128);  new base A (23/22);  total (131/150)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGATC  >  CP009273/2522476‑2522773
                                                                                                                                                    |                                                                                                                                                     
tCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCAcgcg                                                                                                                                                      >  2:3602832/1‑150 (MQ=255)
  aCCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTACACGCTACCGCCCAGCGCGGGGCAcgcgcg                                                                                                                                                    <  2:90256/150‑1 (MQ=255)
   ccAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGa                                                                                                                                                   <  2:859977/150‑1 (MQ=255)
    cAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGAc                                                                                                                                                  <  1:591387/150‑1 (MQ=255)
     aGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACa                                                                                                                                                 <  1:2850847/150‑1 (MQ=255)
        aCGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAg                                                                                                                                              >  1:1467605/1‑150 (MQ=255)
          gCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGcc                                                                                                                                            >  1:117691/1‑150 (MQ=255)
            cTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCaa                                                                                                                                          <  2:3147969/150‑1 (MQ=255)
            cTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCaa                                                                                                                                          <  1:3884740/150‑1 (MQ=255)
            cTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCaa                                                                                                                                          <  2:3461017/150‑1 (MQ=255)
               cAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgct                                                                                                                                       <  1:868369/150‑1 (MQ=255)
                aaCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgctg                                                                                                                                      <  1:335445/150‑1 (MQ=255)
                  cATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgctgct                                                                                                                                    >  1:3927143/1‑150 (MQ=255)
                   aTGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTc                                                                                                                                   <  1:1112915/150‑1 (MQ=255)
                       aCAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCctgc                                                                                                                               <  1:2996919/150‑1 (MQ=255)
                        cAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCctgct                                                                                                                              >  1:395670/1‑150 (MQ=255)
                          ggTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCtgt                                                                                                                            <  1:1305189/150‑1 (MQ=255)
                             tCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGcc                                                                                                                         <  2:2584249/150‑1 (MQ=255)
                               cAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAg                                                                                                                       >  1:4039132/1‑150 (MQ=255)
                                aGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGt                                                                                                                      >  2:1026318/1‑150 (MQ=255)
                                   cgcgCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAg                                                                                                                   >  2:2186452/1‑150 (MQ=255)
                                    gcgcAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGt                                                                                                                  >  1:1949003/1‑150 (MQ=255)
                                    gcgcAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGt                                                                                                                  >  1:1764241/1‑150 (MQ=255)
                                        aaCAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGAc                                                                                                              >  2:2872700/1‑150 (MQ=255)
                                              cGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAAt                                                                                                        >  1:890213/1‑150 (MQ=255)
                                                   tCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcacca                                                                                                   >  2:2636220/1‑150 (MQ=255)
                                                    cgcgCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcaccac                                                                                                  <  2:857513/150‑1 (MQ=255)
                                                     gcgcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACg                                                                                                 <  1:4363720/150‑1 (MQ=255)
                                                     gcgcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACg                                                                                                 <  2:1795491/150‑1 (MQ=255)
                                                       gcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGcc                                                                                               <  1:4113887/150‑1 (MQ=255)
                                                       gcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGcc                                                                                               <  1:3300282/150‑1 (MQ=255)
                                                       gcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGcc                                                                                               <  1:2711266/150‑1 (MQ=255)
                                                             aCGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGat                                                                                         <  1:1664265/150‑1 (MQ=255)
                                                              cGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGata                                                                                        >  2:2446707/1‑150 (MQ=255)
                                                               gCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGatat                                                                                       <  1:1997903/150‑1 (MQ=255)
                                                                   aaCCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCaaa                                                                                   <  1:1414883/150‑1 (MQ=255)
                                                                        gAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGACTTTAAACGCAACCGCCCAGCGCGGGGCACGCGCGACAAAGCAAAGCTGCTCCTGATGAGCCAGTGAGTTGACCATAAACACCACTCCGTCGATATCAAAGCCCa                                                                              >  1:4484473/1‑150 (MQ=255)
                                                                        gAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCa                                                                              >  1:185002/1‑150 (MQ=255)
                                                                         aaaCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAg                                                                             >  2:2045784/1‑150 (MQ=255)
                                                                           aCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCg                                                                           >  2:2758677/1‑150 (MQ=255)
                                                                           aCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCg                                                                           >  2:623423/1‑150 (MQ=255)
                                                                             tCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTc                                                                         >  1:939819/1‑150 (MQ=255)
                                                                             tCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTc                                                                         <  1:746398/150‑1 (MQ=255)
                                                                               gACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCgg                                                                       >  2:4635901/1‑150 (MQ=255)
                                                                               gACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCgg                                                                       >  1:3183294/1‑150 (MQ=255)
                                                                                aCGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCggg                                                                      <  1:2115711/150‑1 (MQ=255)
                                                                                 cGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGc                                                                     <  2:2508409/150‑1 (MQ=255)
                                                                                  gTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCg                                                                    >  1:4575212/1‑150 (MQ=255)
                                                                                    cACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGt                                                                  <  2:3613898/150‑1 (MQ=255)
                                                                                      cGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCACGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGtct                                                                <  2:4303849/150‑1 (MQ=255)
                                                                                        cacaAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCCCGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGtctgc                                                              >  2:1547032/1‑148 (MQ=255)
                                                                                         acaAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGtcttct                                                             >  1:1922002/1‑150 (MQ=255)
                                                                                          caAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGACGTGACCTAAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGtcttcgt                                                            >  1:62113/1‑148 (MQ=255)
                                                                                            aaGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTcc                                                          >  2:1262474/1‑150 (MQ=255)
                                                                                               gTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACt                                                       <  1:3626413/150‑1 (MQ=255)
                                                                                                 cATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACttt                                                     <  2:295036/150‑1 (MQ=255)
                                                                                                  aTCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTtg                                                    <  1:2537035/150‑1 (MQ=255)
                                                                                                    cTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTtgtg                                                  <  1:3841536/150‑1 (MQ=255)
                                                                                                    cTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCCGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTtgtg                                                  >  1:3006084/1‑150 (MQ=255)
                                                                                                     tGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGa                                                 >  2:3401293/1‑150 (MQ=255)
                                                                                                      gCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGAt                                                >  2:4240109/1‑150 (MQ=255)
                                                                                                      gCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGAGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGAt                                                >  1:1044645/1‑150 (MQ=255)
                                                                                                       cTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATa                                               >  2:538133/1‑150 (MQ=255)
                                                                                                       cTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATa                                               <  1:2186740/150‑1 (MQ=255)
                                                                                                         ccTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGa                                             <  2:2902911/150‑1 (MQ=255)
                                                                                                          cTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGaa                                            <  2:1680363/150‑1 (MQ=255)
                                                                                                          cTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGaa                                            >  1:3651294/1‑150 (MQ=255)
                                                                                                           tGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAAc                                           <  2:4599156/150‑1 (MQ=255)
                                                                                                           tGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAAc                                           >  1:2602227/1‑150 (MQ=255)
                                                                                                             cgcCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGc                                         >  2:2255416/1‑150 (MQ=255)
                                                                                                             cgcCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGc                                         <  2:428249/150‑1 (MQ=255)
                                                                                                               ccGGGAATTTAAACGCTACCGCCCAGCGCGGGGCCCGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCa                                       <  1:3996379/150‑1 (MQ=255)
                                                                                                               ccGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCa                                       <  2:3927143/150‑1 (MQ=255)
                                                                                                               ccGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGGGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCa                                       <  2:4368643/150‑1 (MQ=255)
                                                                                                                  ggAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCa                                    >  1:274230/1‑150 (MQ=255)
                                                                                                                   gAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCAc                                   >  1:3744273/1‑150 (MQ=255)
                                                                                                                    aaTTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCACAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCAca                                  >  1:2495624/1‑149 (MQ=255)
                                                                                                                     aTTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCActt                                 <  2:1336507/150‑1 (MQ=255)
                                                                                                                      tttAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCActtc                                <  1:4588525/150‑1 (MQ=255)
                                                                                                                      tttAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCActtc                                >  2:1536981/1‑150 (MQ=255)
                                                                                                                         aaaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACttcttc                             >  2:1193438/1‑150 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            >  2:2807592/1‑150 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            <  1:1555728/150‑1 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            >  2:73566/1‑150 (MQ=255)
                                                                                                                              cTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTccgccg                        >  1:1273885/1‑150 (MQ=255)
                                                                                                                              cTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTccgccg                        >  1:422100/1‑150 (MQ=255)
                                                                                                                              cTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTccgccg                        >  1:4612574/1‑150 (MQ=255)
                                                                                                                                  cGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTc                    <  2:4212918/150‑1 (MQ=255)
                                                                                                                                       tgcgcGTGGCACGAGAGTCAAATCCAAGCTGATCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATATAAGCAAATCGTAGGGATGTCTTATTACACTTTGTGATATAACGCCAGCACTTCTTCCTGCGATTcatttt               <  2:1467553/149‑5 (MQ=255)
                                                                                                                                        gcgcGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAg              <  2:2154366/150‑1 (MQ=255)
                                                                                                                                            ggggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGtt          >  1:351748/1‑150 (MQ=255)
                                                                                                                                             gggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTa         <  1:2338821/150‑1 (MQ=255)
                                                                                                                                             gggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTa         <  1:2338623/150‑1 (MQ=255)
                                                                                                                                              ggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAc        <  1:2902838/150‑1 (MQ=255)
                                                                                                                                              ggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAc        <  1:571774/150‑1 (MQ=255)
                                                                                                                                              ggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAc        <  1:1026318/150‑1 (MQ=255)
                                                                                                                                                cACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAccc      <  2:4006572/150‑1 (MQ=255)
                                                                                                                                                 aCGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCg     >  1:1978978/1‑150 (MQ=255)
                                                                                                                                                  cgcgcgACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGa    <  2:2705474/150‑1 (MQ=255)
                                                                                                                                                    cgcgACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAATCCCAGCGTCGGGCGGTCTTCTTCCAATTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGAGTTACACGATc  <  2:190721/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGATC  >  CP009273/2522476‑2522773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A