Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,522,624 C→A 38.1% A304A (GCG→GCT)  ligA ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,522,6240CA38.1% 345.3 / 255.0 243A304A (GCG→GCT) ligADNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (78/72);  new base A (37/56);  total (115/128)
Fisher's exact test for biased strand distribution p-value = 6.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGATC  >  CP009273/2522476‑2522773
                                                                                                                                                    |                                                                                                                                                     
    cAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGAc                                                                                                                                                  >  2:279874/1‑150 (MQ=255)
      gCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACaa                                                                                                                                                >  2:3587421/1‑150 (MQ=255)
      gCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACaa                                                                                                                                                >  2:3656949/1‑150 (MQ=255)
        aCGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAg                                                                                                                                              <  2:2533017/150‑1 (MQ=255)
            cTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCaa                                                                                                                                          <  2:302365/150‑1 (MQ=255)
               cAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgct                                                                                                                                       <  2:3829213/150‑1 (MQ=255)
                aaCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgctg                                                                                                                                      >  2:2052140/1‑150 (MQ=255)
                aaCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgctg                                                                                                                                      >  1:3527166/1‑150 (MQ=255)
                 aCATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAgctgc                                                                                                                                     >  1:1824771/1‑150 (MQ=255)
                  cATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAgctgct                                                                                                                                    <  1:3494015/150‑1 (MQ=255)
                   aTGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTc                                                                                                                                   >  2:1577216/1‑150 (MQ=255)
                   aTGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTc                                                                                                                                   >  1:1521718/1‑150 (MQ=255)
                   aTGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTc                                                                                                                                   >  2:2408828/1‑150 (MQ=255)
                     ggACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCct                                                                                                                                 >  2:1199658/1‑150 (MQ=255)
                      gACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCctg                                                                                                                                <  1:488342/150‑1 (MQ=255)
                      gACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCctg                                                                                                                                <  2:2575179/150‑1 (MQ=255)
                      gACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCctg                                                                                                                                <  2:1073479/150‑1 (MQ=255)
                       aCAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCctgc                                                                                                                               >  2:245546/1‑150 (MQ=255)
                        cAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCctgct                                                                                                                              >  2:3301518/1‑150 (MQ=255)
                            ttCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGc                                                                                                                          <  2:3259889/150‑1 (MQ=255)
                              ccAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCa                                                                                                                        <  2:3505891/150‑1 (MQ=255)
                                aGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGt                                                                                                                      <  2:1698014/150‑1 (MQ=255)
                                aGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGt                                                                                                                      >  2:22992/1‑150 (MQ=255)
                                   cgcgCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAg                                                                                                                   >  2:1658972/1‑150 (MQ=255)
                                    gcgcAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACCAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGt                                                                                                                  <  1:172862/150‑1 (MQ=255)
                                    gcgcAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGt                                                                                                                  <  1:781887/150‑1 (MQ=255)
                                        aaCAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGAc                                                                                                              >  2:1119925/1‑150 (MQ=255)
                                            ggCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTa                                                                                                          >  2:3933847/1‑150 (MQ=255)
                                             gCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTaa                                                                                                         <  2:3068694/150‑1 (MQ=255)
                                              cGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAAt                                                                                                        <  1:515244/150‑1 (MQ=255)
                                               gTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATc                                                                                                       >  2:2681265/1‑150 (MQ=255)
                                               gTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATc                                                                                                       <  1:1389349/150‑1 (MQ=255)
                                               gTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATc                                                                                                       <  1:3824184/150‑1 (MQ=255)
                                               gTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATc                                                                                                       <  2:1789098/150‑1 (MQ=255)
                                                tAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATca                                                                                                      <  1:1930884/150‑1 (MQ=255)
                                                  aTCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcacc                                                                                                    <  2:2833465/150‑1 (MQ=255)
                                                   tCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcacca                                                                                                   >  2:3448046/1‑150 (MQ=255)
                                                   tCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcacca                                                                                                   <  1:1229390/150‑1 (MQ=255)
                                                   tCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATcacca                                                                                                   <  1:585055/150‑1 (MQ=255)
                                                     gcgcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTTCGCCGGGAATTAAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACg                                                                                                 >  2:1406376/1‑150 (MQ=255)
                                                      cgcCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTATTCACCACGc                                                                                                <  1:3128890/150‑1 (MQ=255)
                                                         cAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGt                                                                                             >  2:469803/1‑150 (MQ=255)
                                                         cAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGt                                                                                             <  2:225085/150‑1 (MQ=255)
                                                         cAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGt                                                                                             >  2:3783011/1‑150 (MQ=255)
                                                           gTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCg                                                                                           <  1:1115950/150‑1 (MQ=255)
                                                           gTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCg                                                                                           <  1:1519046/150‑1 (MQ=255)
                                                                cccAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATc                                                                                      >  1:3658840/1‑150 (MQ=255)
                                                                  cAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCaa                                                                                    >  1:1194606/1‑150 (MQ=255)
                                                                   aaCCTGAAACTCGACGTCACGCACACAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCaaa                                                                                   <  2:418373/150‑1 (MQ=255)
                                                                   aaCCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCaaa                                                                                   >  2:3617008/1‑150 (MQ=255)
                                                                      cTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGcc                                                                                <  2:2660529/150‑1 (MQ=255)
                                                                        gAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCa                                                                              >  1:9823/1‑150 (MQ=255)
                                                                         aaaCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAg                                                                             >  2:1043606/1‑150 (MQ=255)
                                                                         aaaCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAg                                                                             >  2:1043025/1‑150 (MQ=255)
                                                                           aCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCg                                                                           >  1:1352277/1‑150 (MQ=255)
                                                                           aCTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCg                                                                           >  1:3531889/1‑150 (MQ=255)
                                                                             tCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTc                                                                         <  2:1619556/150‑1 (MQ=255)
                                                                             tCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTc                                                                         >  2:3086360/1‑150 (MQ=255)
                                                                                  gTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCg                                                                    <  1:117024/150‑1 (MQ=255)
                                                                                         acaAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGtcttct                                                             >  2:464646/1‑150 (MQ=255)
                                                                                          caAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTcttctt                                                            <  2:4050581/150‑1 (MQ=255)
                                                                                          caAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTcttctt                                                            <  1:1602755/150‑1 (MQ=255)
                                                                                           aaaGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCttcttc                                                           >  1:4123674/1‑150 (MQ=255)
                                                                                            aaGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTcc                                                          >  2:1002337/1‑150 (MQ=255)
                                                                                                tCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACtt                                                      <  2:2148665/150‑1 (MQ=255)
                                                                                                  aTCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTtg                                                    <  1:1285309/150‑1 (MQ=255)
                                                                                                       cTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATa                                               >  1:3379777/1‑150 (MQ=255)
                                                                                                       cTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTAGTGATa                                               >  2:952223/1‑150 (MQ=255)
                                                                                                        tCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAg                                              <  1:603000/150‑1 (MQ=255)
                                                                                                         ccTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGa                                             >  1:4161561/1‑150 (MQ=255)
                                                                                                         ccTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGa                                             <  1:1915084/150‑1 (MQ=255)
                                                                                                           tGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAAc                                           <  2:617578/150‑1 (MQ=255)
                                                                                                           tGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAAc                                           <  1:1118303/150‑1 (MQ=255)
                                                                                                               ccGGGAATTTACACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCa                                       <  2:3012492/150‑1 (MQ=255)
                                                                                                               ccGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCa                                       >  1:2128154/1‑150 (MQ=255)
                                                                                                                  ggAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCATCCACTTTGTGATAGAACGCCAGCa                                    >  1:812475/1‑150 (MQ=255)
                                                                                                                   gAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCAc                                   >  1:1633184/1‑150 (MQ=255)
                                                                                                                       ttAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGACCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCActtct                               <  2:1925071/150‑1 (MQ=255)
                                                                                                                         aaaCGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACttcttc                             >  1:1830987/1‑150 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            <  1:1023480/150‑1 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            >  1:407596/1‑150 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAATCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            <  1:147541/150‑1 (MQ=255)
                                                                                                                          aaCGCTACCGCCCAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTcc                            <  2:1913933/150‑1 (MQ=255)
                                                                                                                             gCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTccgcc                         <  1:279874/150‑1 (MQ=255)
                                                                                                                                aCCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGAt                      >  2:4117561/1‑150 (MQ=255)
                                                                                                                                 ccGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGAtt                     <  1:2511200/150‑1 (MQ=255)
                                                                                                                                  cGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTc                    >  2:2237311/1‑150 (MQ=255)
                                                                                                                                  cGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTc                    <  1:1333776/150‑1 (MQ=255)
                                                                                                                                  cGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTc                    <  1:1171170/150‑1 (MQ=255)
                                                                                                                                     ccAGCGCGGGGCACGCGCGACAAAGCCCAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTcaca                 >  1:2379957/1‑150 (MQ=255)
                                                                                                                                      cAGCGCGGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACaa                >  1:2192547/1‑150 (MQ=255)
                                                                                                                                       aGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACaaa               >  2:3265685/1‑150 (MQ=255)
                                                                                                                                       aGCGCGGGGCACGAGCGACAAAGCAAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACaaa               >  1:536367/1‑150 (MQ=255)
                                                                                                                                          gcgGGGCACGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCg            <  1:306960/150‑1 (MQ=255)
                                                                                                                                            ggggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGtt          >  2:3998755/1‑150 (MQ=255)
                                                                                                                                            ggggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGtt          <  1:3752985/150‑1 (MQ=255)
                                                                                                                                              ggCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAc        <  2:792535/150‑1 (MQ=255)
                                                                                                                                                cACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTAccc      >  2:2417546/1‑150 (MQ=255)
                                                                                                                                                 aCGAGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCg     <  1:3034730/150‑1 (MQ=255)
                                                                                                                                                    cgcgACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGATc  >  1:63969/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTACCGCCCAGCGCGGGGCACGCGCGACAAAGCCAAGCTGCTCCTGCTGTGCCAGTGAGTTGACCTTAATCACCACGCCGTCGATATCAAAGCCCAGCGTCGGGCGGTCTTCTTCCACTTTGTGATAGAACGCCAGCACTTCTTCCGCCGATTCACAAAGCGTTACCCGATC  >  CP009273/2522476‑2522773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A