Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,545,086 C→A 10.8% D138Y (GAT→TAT)  ypeA ← putative acyl‑CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,545,0860CA10.8% 927.1 / 57.3 258D138Y (GAT→TAT) ypeAputative acyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (92/138);  new base A (8/20);  total (100/158)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 276 total aligned reads displayed.

GCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTA  >  CP009273/2544938‑2545234
                                                                                                                                                    |                                                                                                                                                    
gcgcAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACtcttcatct                                                                                                                                                     <  1:3576612/150‑1 (MQ=255)
     gTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTtcaat                                                                                                                                                <  2:285928/150‑1 (MQ=255)
        cccTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTtcaatcaa                                                                                                                                             >  2:2092655/1‑150 (MQ=255)
         ccTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCaaa                                                                                                                                            >  1:152898/1‑150 (MQ=255)
            gCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGc                                                                                                                                         <  2:3535232/150‑1 (MQ=255)
              tCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCtt                                                                                                                                       <  1:2100934/150‑1 (MQ=255)
               cgcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTa                                                                                                                                      >  1:3790419/1‑150 (MQ=255)
               cgcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTa                                                                                                                                      <  2:692123/150‑1 (MQ=255)
               cgcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTa                                                                                                                                      <  1:1820823/150‑1 (MQ=255)
                gcgcgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTAc                                                                                                                                     <  1:1249589/150‑1 (MQ=255)
                   cgACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCa                                                                                                                                  >  2:4456791/1‑150 (MQ=255)
                    gACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAg                                                                                                                                 <  1:1928972/150‑1 (MQ=255)
                      cGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGAc                                                                                                                               <  1:2503751/150‑1 (MQ=255)
                       gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACt                                                                                                                              <  2:1730933/150‑1 (MQ=255)
                       gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACt                                                                                                                              <  1:4149289/150‑1 (MQ=255)
                       gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACt                                                                                                                              <  2:2876602/150‑1 (MQ=255)
                       gCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACt                                                                                                                              <  1:2326395/150‑1 (MQ=255)
                        cACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTc                                                                                                                             >  2:4627578/1‑150 (MQ=255)
                          ccGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGTGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAg                                                                                                                           <  2:1951208/150‑1 (MQ=255)
                          ccGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAg                                                                                                                           <  2:3957783/150‑1 (MQ=255)
                           cGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGc                                                                                                                          >  2:4580821/1‑150 (MQ=255)
                             gCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCAc                                                                                                                        >  1:349922/1‑150 (MQ=255)
                                 tGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCg                                                                                                                    <  2:705492/150‑1 (MQ=255)
                                 tGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCg                                                                                                                    <  1:4336366/150‑1 (MQ=255)
                                  gACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCgg                                                                                                                   >  1:2595095/1‑150 (MQ=255)
                                   aCAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGc                                                                                                                  >  1:2828233/1‑150 (MQ=255)
                                   aCAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGc                                                                                                                  >  2:3673468/1‑150 (MQ=255)
                                    cAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCg                                                                                                                 <  1:1647845/150‑1 (MQ=255)
                                     aaaCAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCgt                                                                                                                >  2:147151/1‑150 (MQ=255)
                                        cAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGtt                                                                                                             >  2:1823866/1‑150 (MQ=255)
                                        cAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGtt                                                                                                             <  1:237300/150‑1 (MQ=255)
                                         aGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTc                                                                                                            <  1:2983155/150‑1 (MQ=255)
                                         aGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTc                                                                                                            >  2:836566/1‑150 (MQ=255)
                                            aCCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCata                                                                                                         <  2:2838082/150‑1 (MQ=255)
                                             cccAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCatat                                                                                                        <  2:1098114/150‑1 (MQ=255)
                                                  gTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAg                                                                                                   <  1:268851/150‑1 (MQ=255)
                                                      gcgcCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGt                                                                                               <  2:2706649/150‑1 (MQ=255)
                                                       cgcCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGtt                                                                                              <  1:4507586/150‑1 (MQ=255)
                                                         ccTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCa                                                                                            <  2:1746568/150‑1 (MQ=255)
                                                         ccTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCa                                                                                            <  2:1746492/150‑1 (MQ=255)
                                                          cTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCAt                                                                                           >  1:2158065/1‑150 (MQ=255)
                                                           tGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATa                                                                                          >  2:2435957/1‑150 (MQ=255)
                                                           tGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATa                                                                                          <  1:2446668/150‑1 (MQ=255)
                                                            gAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATAc                                                                                         >  1:3903166/1‑150 (MQ=255)
                                                            gAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATAc                                                                                         >  1:4584369/1‑150 (MQ=255)
                                                             aaGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACa                                                                                        <  2:967103/150‑1 (MQ=255)
                                                             aaGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACa                                                                                        >  1:2196694/1‑150 (MQ=255)
                                                              aGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACAt                                                                                       >  1:49153/1‑150 (MQ=255)
                                                               gCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACAtt                                                                                      <  2:1559470/150‑1 (MQ=255)
                                                                 aaTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTcc                                                                                    <  1:560097/150‑1 (MQ=255)
                                                                 aaTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTcc                                                                                    >  2:233693/1‑150 (MQ=255)
                                                                 aaTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTcc                                                                                    <  2:1381016/150‑1 (MQ=255)
                                                                  aTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCg                                                                                   >  1:1410592/1‑150 (MQ=255)
                                                                  aTAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCg                                                                                   >  2:1430832/1‑150 (MQ=255)
                                                                    aGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAg                                                                                 <  2:4087376/150‑1 (MQ=255)
                                                                    aGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAg                                                                                 <  1:1995116/150‑1 (MQ=255)
                                                                     gCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGc                                                                                <  2:2650447/150‑1 (MQ=255)
                                                                      cacaAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCa                                                                               >  2:859167/1‑150 (MQ=255)
                                                                         aaGCCAGAAGAGCAAGGGTAACCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCa                                                                            <  2:2052563/150‑1 (MQ=255)
                                                                          aGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACTGGATGAAACTCTGTACTCTTCATCTTCAATCAAACGCTTAGCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCAt                                                                           <  2:917022/150‑1 (MQ=255)
                                                                          aGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCAt                                                                           <  1:2747161/150‑1 (MQ=255)
                                                                              agaagaGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCg                                                                       <  2:439946/150‑1 (MQ=255)
                                                                              agaagaGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCg                                                                       <  2:1876011/150‑1 (MQ=255)
                                                                               gaagaGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGt                                                                      <  1:797063/150‑1 (MQ=255)
                                                                                aagaGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGtt                                                                     <  2:228530/150‑1 (MQ=255)
                                                                                  gagCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTAt                                                                   >  2:2396235/1‑150 (MQ=255)
                                                                                   agCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATc                                                                  <  1:4411850/150‑1 (MQ=255)
                                                                                    gCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCt                                                                 <  2:446770/150‑1 (MQ=255)
                                                                                       aGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTcc                                                              <  2:565112/150‑1 (MQ=255)
                                                                                            aaaCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGcac                                                         <  1:370292/150‑1 (MQ=255)
                                                                                            aaaCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGcac                                                         <  1:402275/150‑1 (MQ=255)
                                                                                            aaaCGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGcac                                                         <  1:529690/150‑1 (MQ=255)
                                                                                               cGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACAtt                                                      >  1:2573888/1‑150 (MQ=255)
                                                                                                    gCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCt                                                 <  1:874289/150‑1 (MQ=255)
                                                                                                    gCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATACCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCt                                                 <  2:2594959/150‑1 (MQ=255)
                                                                                                     cGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGGGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTg                                                >  2:3771501/1‑150 (MQ=255)
                                                                                                          gTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAAttt                                           >  2:4209982/1‑150 (MQ=255)
                                                                                                          gTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAAttt                                           <  1:1378354/150‑1 (MQ=255)
                                                                                                          gTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATATTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAAttt                                           <  1:1163545/150‑1 (MQ=255)
                                                                                                            gcgcATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTc                                         <  1:416166/150‑1 (MQ=255)
                                                                                                             cgcATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCg                                        <  2:3237054/150‑1 (MQ=255)
                                                                                                              gcATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCgg                                       >  1:4415617/1‑150 (MQ=255)
                                                                                                               catcatAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCggg                                      >  1:3462412/1‑150 (MQ=255)
                                                                                                                atcatAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGc                                     >  1:2530658/1‑150 (MQ=255)
                                                                                                                    tAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGcc                                 <  2:3004976/150‑1 (MQ=255)
                                                                                                                     aaTGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCa                                >  1:2610241/1‑150 (MQ=255)
                                                                                                                      aTGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCAc                               <  1:2920334/150‑1 (MQ=255)
                                                                                                                       tGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATATGAATTTTCGGGCAGCCACg                              >  2:1173039/1‑150 (MQ=255)
                                                                                                                             ggATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAAt                        <  1:1833881/150‑1 (MQ=255)
                                                                                                                               aTGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCa                      >  1:305884/1‑150 (MQ=255)
                                                                                                                                    aCTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCttt                 <  2:616053/150‑1 (MQ=255)
                                                                                                                                      tCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCttttt               >  2:3039185/1‑150 (MQ=255)
                                                                                                                                           aCTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCa          <  1:1106825/150‑1 (MQ=255)
                                                                                                                                           aCTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCa          <  2:4033083/150‑1 (MQ=255)
                                                                                                                                            ctctTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAg         <  1:3403015/150‑1 (MQ=255)
                                                                                                                                            ctctTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAg         <  2:1292187/150‑1 (MQ=255)
                                                                                                                                            ctctTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAg         <  1:1078491/150‑1 (MQ=255)
                                                                                                                                             tcttcatcttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGc        >  2:944792/1‑150 (MQ=255)
                                                                                                                                              cttcatcttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGcc       <  1:4456791/150‑1 (MQ=255)
                                                                                                                                                   tcttcaATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTa  <  2:3902910/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCAGTACCCTGCTCGCGCGACGCACCGGCAATGACAAACAGCACCCAGGTTCGCGCCTGAAGCAATAGCACAAGCCAGAAGAGCAAGGGTAAACGCAGGCGACCGTGCGCATCATAATGGACAGGATGAAACTCAGTACTCTTCATCTTCAATCAAACGCTTACCCAGACTCAGCACGTCGGCGTGTTCATATCCCAGGCGTTCATACATTCCGAGCACCATGTCGTTATCTTCCGGCACATTGATCTGAATTTTCGGGCAGCCACGAGCAATCAGCTTTTTCTCCAGCCGATTA  >  CP009273/2544938‑2545234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A