Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,576,567 C→A 30.9% D91Y (GAC→TAC)  nudK ← GDP‑mannose pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,576,5670CA30.9% 553.7 / 221.0 271D91Y (GAC→TAC) nudKGDP‑mannose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (96/91);  new base A (34/50);  total (130/141)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

CTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACA  >  CP009273/2576418‑2576716
                                                                                                                                                     |                                                                                                                                                     
  gATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTcgtcgt                                                                                                                                                     >  2:299511/1‑150 (MQ=255)
   aTTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGtt                                                                                                                                                    <  1:3723942/150‑1 (MQ=255)
   aTTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGtt                                                                                                                                                    <  2:1817844/150‑1 (MQ=255)
      gTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATc                                                                                                                                                 >  2:1376628/1‑150 (MQ=255)
       tCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATcc                                                                                                                                                <  1:1213148/150‑1 (MQ=255)
        cACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCca                                                                                                                                               >  2:2088271/1‑150 (MQ=255)
        cACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCca                                                                                                                                               <  1:3860017/150‑1 (MQ=255)
         aCTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCcag                                                                                                                                              <  2:710395/150‑1 (MQ=255)
           tGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCcagca                                                                                                                                            >  2:4309478/1‑150 (MQ=255)
             tATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCagcagc                                                                                                                                          <  2:3582360/150‑1 (MQ=255)
               ttCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGccc                                                                                                                                        <  2:2947877/150‑1 (MQ=255)
                 cGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCgg                                                                                                                                      >  1:4217214/1‑150 (MQ=255)
                     gATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCa                                                                                                                                  <  1:3779017/150‑1 (MQ=255)
                     gATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCa                                                                                                                                  <  1:879594/150‑1 (MQ=255)
                        aaaaaaGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCt                                                                                                                               >  1:4242316/1‑150 (MQ=255)
                        aaaaaaGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACGTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCt                                                                                                                               <  2:4214535/150‑1 (MQ=255)
                          aaaaGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCttt                                                                                                                             <  1:3687719/150‑1 (MQ=255)
                            aaGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCa                                                                                                                           <  1:795307/150‑1 (MQ=255)
                            aaGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCa                                                                                                                           >  1:2498605/1‑150 (MQ=255)
                              gTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAAt                                                                                                                         >  1:2432180/1‑150 (MQ=255)
                               tGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATc                                                                                                                        >  2:2650840/1‑150 (MQ=255)
                                  aTTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGc                                                                                                                     >  1:3098486/1‑150 (MQ=255)
                                  aTTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGc                                                                                                                     >  1:3572929/1‑150 (MQ=255)
                                   ttAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCt                                                                                                                    <  2:1969204/150‑1 (MQ=255)
                                   ttAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCt                                                                                                                    <  2:279731/150‑1 (MQ=255)
                                    tAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTg                                                                                                                   >  2:4044477/1‑150 (MQ=255)
                                     aGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGc                                                                                                                  <  2:985400/150‑1 (MQ=255)
                                      gCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGcc                                                                                                                 >  1:322422/1‑150 (MQ=255)
                                         cAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGc                                                                                                              >  1:2778891/1‑150 (MQ=255)
                                           gTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCtt                                                                                                            <  1:484651/150‑1 (MQ=255)
                                           gTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCtt                                                                                                            <  1:4404937/150‑1 (MQ=255)
                                             cacaCCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTc                                                                                                          <  2:1166557/150‑1 (MQ=255)
                                              acacCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCa                                                                                                         <  2:1699843/150‑1 (MQ=255)
                                               cacCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCAt                                                                                                        <  2:2039455/150‑1 (MQ=255)
                                                acCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCAtt                                                                                                       <  1:258298/150‑1 (MQ=255)
                                                  cGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGc                                                                                                     <  2:1891756/150‑1 (MQ=255)
                                                       ggCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTa                                                                                                >  2:1747128/1‑150 (MQ=255)
                                                         cGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAAc                                                                                              >  2:1539995/1‑150 (MQ=255)
                                                         cGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAAc                                                                                              <  2:639078/150‑1 (MQ=255)
                                                         cGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAAc                                                                                              <  2:214697/150‑1 (MQ=255)
                                                          gACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAAcc                                                                                             <  2:4243383/150‑1 (MQ=255)
                                                           aCATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCGTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAAccc                                                                                            <  2:575724/150‑1 (MQ=255)
                                                               ataCAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGt                                                                                        >  2:1451120/1‑150 (MQ=255)
                                                                taCAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTa                                                                                       <  1:3162617/150‑1 (MQ=255)
                                                                   aGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCg                                                                                    <  1:714329/150‑1 (MQ=255)
                                                                     ttCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGac                                                                                  <  2:47793/150‑1 (MQ=255)
                                                                        aaaTAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACg                                                                               >  2:3768068/1‑150 (MQ=255)
                                                                        aaaTAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACg                                                                               >  2:3768022/1‑150 (MQ=255)
                                                                        aaaTAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACg                                                                               <  1:4539763/150‑1 (MQ=255)
                                                                         aataatTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTCGCGACACgg                                                                              >  1:1067211/1‑150 (MQ=255)
                                                                                tGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGAc                                                                       <  2:2241572/150‑1 (MQ=255)
                                                                                  cgcACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGa                                                                     <  1:2413927/150‑1 (MQ=255)
                                                                                    cACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAAt                                                                   <  2:2432180/150‑1 (MQ=255)
                                                                                     aCTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATc                                                                  <  1:1462049/150‑1 (MQ=255)
                                                                                      gttCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCa                                                                 >  1:2868707/2‑150 (MQ=255)
                                                                                      cTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCa                                                                 <  2:534191/150‑1 (MQ=255)
                                                                                      cTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCa                                                                 >  1:2868465/1‑150 (MQ=255)
                                                                                       ttCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAg                                                                <  1:299511/150‑1 (MQ=255)
                                                                                       ttCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAg                                                                <  1:4050296/150‑1 (MQ=255)
                                                                                          aCCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAAc                                                             >  1:1809304/1‑150 (MQ=255)
                                                                                           cccACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAAcc                                                            >  1:2930747/1‑150 (MQ=255)
                                                                                           cccACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAAcc                                                            <  1:1330810/150‑1 (MQ=255)
                                                                                            ccACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCa                                                           >  2:515430/1‑150 (MQ=255)
                                                                                             cACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCAc                                                          <  2:1074244/150‑1 (MQ=255)
                                                                                                ttCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGt                                                       <  2:2917065/150‑1 (MQ=255)
                                                                                                 tCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTc                                                      <  2:1596942/150‑1 (MQ=255)
                                                                                                 tCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTc                                                      >  2:1227248/1‑150 (MQ=255)
                                                                                                  cATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCt                                                     >  1:4574089/1‑150 (MQ=255)
                                                                                                    tAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCttt                                                   <  2:784022/150‑1 (MQ=255)
                                                                                                    tAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCttt                                                   >  1:1118667/1‑150 (MQ=255)
                                                                                                      gCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCttttt                                                 >  2:440763/1‑150 (MQ=255)
                                                                                                       cccGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTttc                                                >  1:2268/1‑150 (MQ=255)
                                                                                                        ccGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTttct                                               >  1:4606506/1‑150 (MQ=255)
                                                                                                         cGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTttctt                                              <  1:2645467/150‑1 (MQ=255)
                                                                                                          gTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTttcttc                                             >  2:4484645/1‑150 (MQ=255)
                                                                                                           tctcTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCg                                            <  2:1678076/150‑1 (MQ=255)
                                                                                                           tctcTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCg                                            >  2:2606625/1‑150 (MQ=255)
                                                                                                               ttCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGtgtt                                        <  1:4531177/150‑1 (MQ=255)
                                                                                                                tCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGtgttg                                       <  1:3313013/150‑1 (MQ=255)
                                                                                                                tCAATCGCTTCTTAGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGtgttg                                       >  1:4163352/1‑150 (MQ=255)
                                                                                                                 cAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGtgttgt                                      <  2:156693/150‑1 (MQ=255)
                                                                                                                    tCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACa                                   >  1:1975713/1‑150 (MQ=255)
                                                                                                                      gCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACagg                                 >  1:1509050/1‑150 (MQ=255)
                                                                                                                         tcttTGCGAATACACACTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACaggagg                              <  1:2459842/150‑1 (MQ=255)
                                                                                                                          cttTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAggagga                             >  2:3452565/1‑150 (MQ=255)
                                                                                                                            ttGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGAtc                           <  2:3699056/150‑1 (MQ=255)
                                                                                                                               cGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGAtcgtc                        >  1:449262/1‑150 (MQ=255)
                                                                                                                               cGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGAtcgtc                        <  1:2169829/150‑1 (MQ=255)
                                                                                                                                  aTACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCa                     >  1:1732118/1‑150 (MQ=255)
                                                                                                                                  aTACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCa                     >  1:3130351/1‑150 (MQ=255)
                                                                                                                                    acacacTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCAcc                   <  2:255904/150‑1 (MQ=255)
                                                                                                                                      acacTTCCGGTTCGTAGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCAt                 >  1:1607112/1‑150 (MQ=255)
                                                                                                                                        acTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTg               >  1:3911216/1‑150 (MQ=255)
                                                                                                                                        acTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTg               >  2:1399028/1‑150 (MQ=255)
                                                                                                                                           tCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCcg            <  1:1374923/150‑1 (MQ=255)
                                                                                                                                           tCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCcg            <  2:3989595/150‑1 (MQ=255)
                                                                                                                                             cGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCcgcg          >  1:4484898/1‑150 (MQ=255)
                                                                                                                                               ggtcgtcgTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGAt        <  1:1925601/148‑1 (MQ=255)
                                                                                                                                                 tcgtcgTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCg      <  2:322422/150‑1 (MQ=255)
                                                                                                                                                     cgtTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTaca  >  1:189005/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCCTGGCGACATATACAGTTCAAATAATTTGCGCACTTCACCCACTTCATAGCCCGTCTCTTCAATCGCTTCTTTGCGAATACACACTTCCGGTTCGTCGTTATCCAGCAGCCCGGCGCAGCTTTCAATCAGCTGCCCGCTTTCATTGCCATTAACCCAGGTAGCGACACGGAACTGACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACA  >  CP009273/2576418‑2576716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A