Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,721 C→A 40.2% R56S (CGT→AGT)  purM → phosphoribosylaminoimidazole synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,7210CA40.2% 345.3 / 306.4 271R56S (CGT→AGT) purMphosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base C (86/76);  new base A (57/52);  total (143/128)
Fisher's exact test for biased strand distribution p-value = 9.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

CTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTC  >  CP009273/2614572‑2614870
                                                                                                                                                     |                                                                                                                                                     
ctctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATc                                                                                                                                                       >  2:3184266/1‑150 (MQ=255)
 tctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                                                                                      <  1:2255642/150‑1 (MQ=255)
   ttAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTg                                                                                                                                                    <  2:930947/150‑1 (MQ=255)
    tAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                                                                                                   <  1:1192263/150‑1 (MQ=255)
    tAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGa                                                                                                                                                   <  1:3934931/150‑1 (MQ=255)
         aCAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCg                                                                                                                                              <  2:3470172/150‑1 (MQ=255)
          cAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGt                                                                                                                                             <  1:1746066/150‑1 (MQ=255)
           aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTg                                                                                                                                            >  2:3310225/1‑150 (MQ=255)
           aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTg                                                                                                                                            <  2:2279982/150‑1 (MQ=255)
                  ccGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                                                                                                     <  2:769889/150‑1 (MQ=255)
                  ccGGTGTTGATATGGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGttt                                                                                                                                     <  2:1710588/150‑1 (MQ=255)
                     gtgtTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTg                                                                                                                                  <  1:3061266/150‑1 (MQ=255)
                       gtTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGc                                                                                                                                <  2:2197049/150‑1 (MQ=255)
                         tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCAc                                                                                                                              >  1:4446418/1‑150 (MQ=255)
                          gATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACt                                                                                                                             >  2:2904687/1‑150 (MQ=255)
                           atatTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTg                                                                                                                            <  2:3213275/150‑1 (MQ=255)
                              ttGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACg                                                                                                                         <  2:3336873/150‑1 (MQ=255)
                                gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGc                                                                                                                       >  1:3829225/1‑150 (MQ=255)
                                     gggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAgg                                                                                                                  <  1:3414803/150‑1 (MQ=255)
                                         aaTGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTAcc                                                                                                              >  1:1247787/1‑150 (MQ=255)
                                         aaTGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTAcc                                                                                                              <  1:3677822/150‑1 (MQ=255)
                                              tctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                                                                                         >  1:780009/1‑150 (MQ=255)
                                              tctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                                                                                         <  2:434516/150‑1 (MQ=255)
                                               ctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTg                                                                                                        >  2:2800225/1‑150 (MQ=255)
                                               ctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTg                                                                                                        >  2:2799087/1‑150 (MQ=255)
                                               ctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTg                                                                                                        >  1:2464261/1‑150 (MQ=255)
                                                 ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCg                                                                                                      >  1:2635146/1‑150 (MQ=255)
                                                   ttGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTc                                                                                                    <  1:2156614/150‑1 (MQ=255)
                                                       aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTTCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGc                                                                                                >  1:364113/1‑150 (MQ=255)
                                                       aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGc                                                                                                >  2:2380801/1‑150 (MQ=255)
                                                        agaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCa                                                                                               >  1:4311924/1‑150 (MQ=255)
                                                        agaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCa                                                                                               >  2:4500123/1‑150 (MQ=255)
                                                          aaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                                                                                             >  2:2640424/1‑150 (MQ=255)
                                                          aaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                                                                                             <  1:1432394/150‑1 (MQ=255)
                                                          aaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                                                                                             <  1:509926/150‑1 (MQ=255)
                                                             cAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGa                                                                                          >  1:269009/1‑150 (MQ=255)
                                                               aaGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACt                                                                                        >  1:2317109/1‑150 (MQ=255)
                                                                aGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACtt                                                                                       <  1:41761/150‑1 (MQ=255)
                                                                 ggCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGTATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTa                                                                                      >  2:3244171/1‑150 (MQ=255)
                                                                 ggCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTa                                                                                      <  1:3298909/150‑1 (MQ=255)
                                                                   cGTAGTGACGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaa                                                                                    >  2:3048122/1‑150 (MQ=255)
                                                                   cGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaa                                                                                    >  2:3369878/1‑150 (MQ=255)
                                                                    gTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaaa                                                                                   <  1:833516/150‑1 (MQ=255)
                                                                     tAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAAc                                                                                  <  2:1880290/150‑1 (MQ=255)
                                                                      aGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACg                                                                                 <  1:2791327/150‑1 (MQ=255)
                                                                      aGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACg                                                                                 >  1:2981184/1‑150 (MQ=255)
                                                                      aGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACg                                                                                 <  2:4092742/150‑1 (MQ=255)
                                                                         gaagaaAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa                                                                              >  1:1613386/1‑150 (MQ=255)
                                                                             aaaaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac                                                                          <  1:188131/150‑1 (MQ=255)
                                                                             aaaaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac                                                                          <  1:605214/150‑1 (MQ=255)
                                                                              aaaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGaca                                                                         >  2:3594866/1‑150 (MQ=255)
                                                                                aCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGGGCTGTGTGCATTGCCGTAAAAATATCGTGTACGCTTGCTGGTTTCTGGAAATGACGGCGTAGGTAACAAGCTGCGTATGGCAATGGTCTTAAAAAGTCAGGagacg                                                                       <  2:2111224/150‑2 (MQ=255)
                                                                                 cgcgTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCa                                                                      >  2:4547416/1‑150 (MQ=255)
                                                                                 cgcgTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCa                                                                      <  1:2015143/150‑1 (MQ=255)
                                                                                  gcgTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCag                                                                     >  2:358067/1‑149 (MQ=255)
                                                                                   cgtcgtCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCAtt                                                                    <  2:3278013/150‑1 (MQ=255)
                                                                                    gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCTGTGCGCTGTGTGCATTGACGCAAAAATATATTGAACCCGTGCTGGTTTCTGGCACTTACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTg                                                                   >  2:3992257/1‑150 (MQ=255)
                                                                                    gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTg                                                                   <  2:780311/150‑1 (MQ=255)
                                                                                    gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGGAGGTACCAAGCTGCGACTGGCAATGGACTTAAAACGTCACGACACCATTg                                                                   >  1:3986527/1‑150 (MQ=255)
                                                                                     tcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTgg                                                                  >  1:882523/1‑150 (MQ=255)
                                                                                      cgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATAGGt                                                                 >  2:549168/1‑150 (MQ=255)
                                                                                          cGGAAGTGCTGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTTGTTTCTGGCACTGACGGCGTATGTACCAAGCTGCGTCTGTCAATGGACTTAAATCGTCACGACACCATTGGTATTg                                                             <  2:1294290/150‑1 (MQ=255)
                                                                                           ggAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGa                                                            <  2:193979/150‑1 (MQ=255)
                                                                                           ggAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGa                                                            >  1:2519600/1‑150 (MQ=255)
                                                                                            gAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGAt                                                           <  2:4339411/150‑1 (MQ=255)
                                                                                              aGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCt                                                         >  2:1605965/1‑150 (MQ=255)
                                                                                                 gATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGt                                                      <  1:1724081/150‑1 (MQ=255)
                                                                                                        ggTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCAtg                                               >  1:2442633/1‑150 (MQ=255)
                                                                                                          tCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCAtgtg                                             <  2:419995/150‑1 (MQ=255)
                                                                                                            tGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCg                                           <  2:3408948/150‑1 (MQ=255)
                                                                                                             gggCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGt                                          >  1:993572/1‑150 (MQ=255)
                                                                                                             gggCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGt                                          >  1:981480/1‑150 (MQ=255)
                                                                                                             gggCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGt                                          >  2:4207537/1‑150 (MQ=255)
                                                                                                               gcggcTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTa                                        >  2:2985395/1‑150 (MQ=255)
                                                                                                                cggcTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTaa                                       >  2:3873550/1‑150 (MQ=255)
                                                                                                                cggcTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTaa                                       >  2:2491156/1‑150 (MQ=255)
                                                                                                                 ggcTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAAt                                      >  2:947574/1‑150 (MQ=255)
                                                                                                                  gcTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATg                                     <  1:1486618/150‑1 (MQ=255)
                                                                                                                   cTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGa                                    >  2:2177614/1‑150 (MQ=255)
                                                                                                                    ttCGGTGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGAc                                   <  1:934583/150‑1 (MQ=255)
                                                                                                                     tCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGAcc                                  <  2:3925287/150‑1 (MQ=255)
                                                                                                                        gTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtgg                               >  2:2507744/1‑150 (MQ=255)
                                                                                                                         tGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtggt                              >  2:3756285/1‑150 (MQ=255)
                                                                                                                         tGCGCTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtggt                              >  1:715083/1‑150 (MQ=255)
                                                                                                                          gcgcTGTGTGCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtggtg                             <  1:2981733/150‑1 (MQ=255)
                                                                                                                           ctcTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtggtgg                            <  2:1616380/148‑1 (MQ=255)
                                                                                                                           cgcTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCtggtgg                            >  1:46300/1‑150 (MQ=255)
                                                                                                                            gcTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTggtggt                           >  1:2971066/1‑150 (MQ=255)
                                                                                                                              tgtgtgCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGc                         >  1:1055993/1‑150 (MQ=255)
                                                                                                                                 gtgCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAg                      >  1:2838599/1‑150 (MQ=255)
                                                                                                                                 gtgCATTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAg                      >  1:238943/1‑150 (MQ=255)
                                                                                                                                   gCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGt                    >  2:3799466/1‑150 (MQ=255)
                                                                                                                                     aTTGCCGCAAAAATATAGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGc                  <  2:4376608/150‑1 (MQ=255)
                                                                                                                                       tGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCag                <  2:2499548/150‑1 (MQ=255)
                                                                                                                                         ccGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCagag              <  2:1432973/150‑1 (MQ=255)
                                                                                                                                             aaaaaTATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGc          <  1:3314165/150‑1 (MQ=255)
                                                                                                                                                 atatCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGtt      <  2:3548172/150‑1 (MQ=255)
                                                                                                                                                 atatCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGtt      >  2:2050106/1‑150 (MQ=255)
                                                                                                                                                  tatCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGttt     <  1:2117130/150‑1 (MQ=255)
                                                                                                                                                     cGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTc  >  1:1369639/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACACCATTGGTATTGATCTGGTCGCCATGTGCGTTAATGACCTGGTGGTGCAAGGTGCAGAGCCGCTGTTTTTC  >  CP009273/2614572‑2614870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A