Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,615,370 C→A 41.7% S272Y (TCT→TAT)  purM → phosphoribosylaminoimidazole synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,615,3700CA41.7% 270.5 / 286.2 242S272Y (TCT→TAT) purMphosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base C (79/62);  new base A (51/50);  total (130/112)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAAT  >  CP009273/2615224‑2615518
                                                                                                                                                  |                                                                                                                                                    
ggCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTc                                                                                                                                                   <  1:718792/150‑1 (MQ=255)
ggCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTc                                                                                                                                                   <  2:3468561/150‑1 (MQ=255)
  cACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCt                                                                                                                                                 <  1:1276657/150‑1 (MQ=255)
    ccGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTgg                                                                                                                                               >  2:1241074/1‑150 (MQ=255)
     cGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGc                                                                                                                                              >  1:3758015/1‑150 (MQ=255)
        cccGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGt                                                                                                                                           >  2:676908/1‑150 (MQ=255)
         ccGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACATGACCGGCGGCGGCTTCTGGGAAAACATTCAGCGCGTATAGCCAGATAATACTCAGGCAGTGATTGATGAATCATCCTGGCAGTg                                                                                                                                          >  1:2388526/1‑150 (MQ=255)
          cGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTgg                                                                                                                                         <  1:1053593/150‑1 (MQ=255)
            cATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGcc                                                                                                                                       >  2:2063024/1‑150 (MQ=255)
               ttACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCATATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGa                                                                                                                                    <  1:2264217/150‑1 (MQ=255)
                 aCGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAg                                                                                                                                  <  2:2081749/150‑1 (MQ=255)
                  cGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAgt                                                                                                                                 >  2:213399/1‑150 (MQ=255)
                   gTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAgtg                                                                                                                                >  1:3577418/1‑150 (MQ=255)
                   gTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAgtg                                                                                                                                <  2:2236386/150‑1 (MQ=255)
                     gAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGtt                                                                                                                              >  1:1041/1‑150 (MQ=255)
                     gAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGtt                                                                                                                              >  1:946/1‑150 (MQ=255)
                          cAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACt                                                                                                                         >  1:1694656/1‑150 (MQ=255)
                          cAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACt                                                                                                                         >  2:3242743/1‑150 (MQ=255)
                                 ggAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCa                                                                                                                  >  2:2522227/1‑150 (MQ=255)
                                 ggAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCa                                                                                                                  >  2:2522299/1‑150 (MQ=255)
                                  gAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCaa                                                                                                                 >  1:1181926/1‑150 (MQ=255)
                                      tgattgaAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACgg                                                                                                             <  1:3175263/150‑1 (MQ=255)
                                      tgattgaAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACgg                                                                                                             <  1:3730319/150‑1 (MQ=255)
                                           gaAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGt                                                                                                        <  1:3303026/150‑1 (MQ=255)
                                           gaAAAGGTCGAGGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGt                                                                                                        <  1:159672/150‑1 (MQ=255)
                                             aaaGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTaa                                                                                                      <  1:2989878/150‑1 (MQ=255)
                                               aGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACg                                                                                                    >  2:2417720/1‑150 (MQ=255)
                                                ggTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGt                                                                                                   >  1:2281512/1‑150 (MQ=255)
                                                ggTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGt                                                                                                   >  2:3285740/1‑150 (MQ=255)
                                                  tCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTg                                                                                                 <  1:393428/150‑1 (MQ=255)
                                                  tCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGGGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTg                                                                                                 <  1:393407/150‑1 (MQ=255)
                                                   cGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGa                                                                                                <  2:3065396/150‑1 (MQ=255)
                                                   cGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGa                                                                                                <  2:2665619/150‑1 (MQ=255)
                                                    gATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAg                                                                                               >  1:1902266/1‑150 (MQ=255)
                                                    gATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAg                                                                                               <  2:1725507/150‑1 (MQ=255)
                                                          cATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCAt                                                                                         <  1:3657858/150‑1 (MQ=255)
                                                          cATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAAACTTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCAt                                                                                         <  1:41264/150‑1 (MQ=255)
                                                           aTGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATg                                                                                        >  2:3806380/1‑150 (MQ=255)
                                                               cATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAAt                                                                                    >  1:2258076/1‑150 (MQ=255)
                                                               cATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAAt                                                                                    <  2:2827442/150‑1 (MQ=255)
                                                                 ttGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGt                                                                                  <  1:1356901/150‑1 (MQ=255)
                                                                    cgcACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATc                                                                               <  2:2999320/150‑1 (MQ=255)
                                                                    cgcACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATc                                                                               >  1:1887218/1‑150 (MQ=255)
                                                                      cACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGc                                                                             >  1:1395791/1‑150 (MQ=255)
                                                                       aCCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCa                                                                            >  2:1437755/1‑150 (MQ=255)
                                                                         cTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCAcc                                                                          >  1:882665/1‑150 (MQ=255)
                                                                          tGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCt                                                                         >  2:548440/1‑150 (MQ=255)
                                                                          tGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCt                                                                         >  1:2579173/1‑150 (MQ=255)
                                                                          tGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCt                                                                         >  1:2550042/1‑150 (MQ=255)
                                                                                gcggcggcTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACt                                                                   <  1:1447162/150‑1 (MQ=255)
                                                                                  ggcggcTTCTGGGAAAACATTCCGCGCGTAGTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTgc                                                                 <  1:1124957/150‑1 (MQ=255)
                                                                                   gcggcTTCTGGGAAAACATTCCGCGCGTAGTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTgcg                                                                <  1:3156641/150‑1 (MQ=255)
                                                                                    cggcTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTgcgg                                                               >  2:1834107/1‑150 (MQ=255)
                                                                                     ggcTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTgcggc                                                              >  2:3789920/1‑150 (MQ=255)
                                                                                      gcTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTgcggcg                                                             <  2:1331074/150‑1 (MQ=255)
                                                                                        ttCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTc                                                           <  2:4064333/150‑1 (MQ=255)
                                                                                         tCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCg                                                          >  1:1234554/1‑150 (MQ=255)
                                                                                         tCTGGGAAAACATTCCGCGCGGATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCg                                                          <  2:1983581/150‑1 (MQ=255)
                                                                                            gggAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGga                                                       >  1:4078862/1‑150 (MQ=255)
                                                                                              gAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGgatg                                                     >  1:2434446/1‑150 (MQ=255)
                                                                                                 aaCATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGatt                                                  >  1:2839684/1‑150 (MQ=255)
                                                                                                 aaCATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGatt                                                  >  2:151004/1‑150 (MQ=255)
                                                                                                 aaCATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGatt                                                  >  2:1626636/1‑150 (MQ=255)
                                                                                                 aaCATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGatt                                                  <  1:492055/150‑1 (MQ=255)
                                                                                                    aTTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGattatt                                               >  1:3232748/1‑150 (MQ=255)
                                                                                                     ttCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATAGTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTg                                              <  1:3447654/150‑1 (MQ=255)
                                                                                                              tATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCcctgcctg                                     <  1:221972/150‑1 (MQ=255)
                                                                                                              tATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCcctgcctg                                     >  1:3509250/1‑150 (MQ=255)
                                                                                                              tATTGCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCcctgcctg                                     <  2:887815/150‑1 (MQ=255)
                                                                                                                 tGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTc                                  <  2:3668986/150‑1 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGTATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTNCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 >  1:456010/1‑150 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGTTAACGTTGAGCAACATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 >  1:339468/1‑150 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 >  1:1211008/1‑150 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 >  1:628261/1‑150 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 <  1:3068828/150‑1 (MQ=255)
                                                                                                                  gCCAGATAATACTCAGGCAGTGATTGATGAATATTCCGGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTcc                                 <  1:1637462/150‑1 (MQ=255)
                                                                                                                     aGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGa                              >  1:1252903/1‑150 (MQ=255)
                                                                                                                      gATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGaa                             >  2:4108754/1‑150 (MQ=255)
                                                                                                                       ataataCTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAg                            >  1:1672038/1‑150 (MQ=255)
                                                                                                                         aataCTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTg                          <  2:1762510/150‑1 (MQ=255)
                                                                                                                          ataCTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTgg                         >  2:3067812/1‑150 (MQ=255)
                                                                                                                          ataCTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTgg                         >  1:1907438/1‑150 (MQ=255)
                                                                                                                           taCTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGa                        <  1:2335329/150‑1 (MQ=255)
                                                                                                                           taCTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGa                        <  2:1888373/150‑1 (MQ=255)
                                                                                                                           taCTCAGGCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGa                        <  2:3987056/150‑1 (MQ=255)
                                                                                                                            aCTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGAc                       >  1:3441984/1‑150 (MQ=255)
                                                                                                                               cAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACaaa                    >  2:3378344/1‑150 (MQ=255)
                                                                                                                                  gCAGTGATTGATGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGcc                 <  1:483473/150‑1 (MQ=255)
                                                                                                                                    aGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGTCCTGCCTGCTCCGGAAGTGGACAAAGCCCt               >  1:2963736/1‑150 (MQ=255)
                                                                                                                                    aGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCt               <  2:2655262/150‑1 (MQ=255)
                                                                                                                                    aGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCt               >  1:585315/1‑150 (MQ=255)
                                                                                                                                      tgattgatGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCg             <  2:3869267/150‑1 (MQ=255)
                                                                                                                                       gattgatGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGc            >  1:1489547/1‑150 (MQ=255)
                                                                                                                                         ttgatGAATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTAGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGccc          >  2:3136258/1‑150 (MQ=255)
                                                                                                                                          tgatgaATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCt         >  2:125131/1‑150 (MQ=255)
                                                                                                                                           gatgaATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTg        >  1:1021907/1‑150 (MQ=255)
                                                                                                                                             tgaATATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCt      <  2:1601853/150‑1 (MQ=255)
                                                                                                                                               aaTCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCa    <  2:821561/150‑1 (MQ=255)
                                                                                                                                               aaTATTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCa    >  1:2980362/1‑150 (MQ=255)
                                                                                                                                                 tCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAAt  <  2:2828767/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGATGAATCTTCCTGGCAGTGGCCGGAAGTGTTCAACTGGCTGCAAACGGCAGGTAACGTTGAGCACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAAT  >  CP009273/2615224‑2615518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A