Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,617,515 T→G 5.5% I371M (ATT→ATG)  ppk → polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,617,5150TG 5.5% 790.7 / 33.1 253I371M (ATT→ATG) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base T (109/128);  new base G (4/10);  total (115/138)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

ATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAA  >  CP009273/2617366‑2617662
                                                                                                                                                     |                                                                                                                                                   
atTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGGACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAAtt                                                                                                                                                     >  1:1464098/1‑150 (MQ=255)
   tGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAAc                                                                                                                                                  >  1:2935245/1‑150 (MQ=255)
     gTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACAt                                                                                                                                                >  1:2694529/1‑150 (MQ=255)
     gTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACAt                                                                                                                                                >  1:192413/1‑150 (MQ=255)
       ttGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACAttt                                                                                                                                              >  2:150340/1‑150 (MQ=255)
       ttGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACAttt                                                                                                                                              <  1:43828/150‑1 (MQ=255)
        tGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTa                                                                                                                                             <  1:165520/150‑1 (MQ=255)
            aaaGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACcgc                                                                                                                                         <  1:3393249/150‑1 (MQ=255)
             aaGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACcgcg                                                                                                                                        >  1:1025659/1‑150 (MQ=255)
              aGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGt                                                                                                                                       >  1:647746/1‑150 (MQ=255)
                cccAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTgg                                                                                                                                     <  1:394943/150‑1 (MQ=255)
                  cAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCg                                                                                                                                   <  1:497911/150‑1 (MQ=255)
                   aGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGa                                                                                                                                  >  2:3874662/1‑150 (MQ=255)
                   aGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGa                                                                                                                                  >  2:4538125/1‑150 (MQ=255)
                   aGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGa                                                                                                                                  >  2:4482561/1‑150 (MQ=255)
                    gTTCCGCTATGGTTTTGTTGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGATCTGCTGCGTCAGGCTTCGTTCGACCAGAGCGTACTGGCTATTATAATGAACATTTACCGCGTGGCGaa                                                                                                                                 <  2:488722/150‑1 (MQ=255)
                    gTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGaa                                                                                                                                 >  1:3948093/1‑150 (MQ=255)
                      tCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAg                                                                                                                               <  1:3518455/150‑1 (MQ=255)
                        cGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGAt                                                                                                                             >  2:1048489/1‑150 (MQ=255)
                        cGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGAt                                                                                                                             >  2:2993747/1‑150 (MQ=255)
                        cGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGAt                                                                                                                             >  2:2562838/1‑150 (MQ=255)
                        cGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATGAACATTTACCGCGTGGCGAAAGAt                                                                                                                             >  2:331226/1‑150 (MQ=255)
                         gCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGAtt                                                                                                                            >  1:956131/1‑150 (MQ=255)
                         gCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGAtt                                                                                                                            <  1:3354073/150‑1 (MQ=255)
                             tGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACg                                                                                                                        <  1:574623/150‑1 (MQ=255)
                              ggTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGc                                                                                                                       >  1:2154712/1‑150 (MQ=255)
                               gTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGca                                                                                                                      >  2:1984532/1‑150 (MQ=255)
                                   tGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGcatcat                                                                                                                  <  1:3089405/150‑1 (MQ=255)
                                   tGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGcatcat                                                                                                                  >  2:1616596/1‑150 (MQ=255)
                                     aTGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCg                                                                                                                <  1:1276107/150‑1 (MQ=255)
                                     aTGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCg                                                                                                                >  1:3698153/1‑150 (MQ=255)
                                      tGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGa                                                                                                               >  1:2863244/1‑150 (MQ=255)
                                         cATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTc                                                                                                            >  1:4295188/1‑150 (MQ=255)
                                             cgcgAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCgatg                                                                                                        <  1:3779104/150‑1 (MQ=255)
                                             cgcgAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCgatg                                                                                                        <  1:25317/150‑1 (MQ=255)
                                                gAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATc                                                                                                     >  2:2687904/1‑150 (MQ=255)
                                                gAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATc                                                                                                     >  2:3993146/1‑150 (MQ=255)
                                                 aaCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATcc                                                                                                    <  1:3557649/150‑1 (MQ=255)
                                                 aaCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATcc                                                                                                    >  1:4310122/1‑150 (MQ=255)
                                                 aaCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATcc                                                                                                    <  2:3287204/150‑1 (MQ=255)
                                                  aCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCa                                                                                                   <  2:2713939/150‑1 (MQ=255)
                                                  aCGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCa                                                                                                   <  2:3249474/150‑1 (MQ=255)
                                                   cgcgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAc                                                                                                  <  2:85968/150‑1 (MQ=255)
                                                   cgcgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAc                                                                                                  >  2:1275672/1‑150 (MQ=255)
                                                   cgcgATGTGTTGCTCTATTATCCTTATAACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAc                                                                                                  >  1:1782137/1‑150 (MQ=255)
                                                    gcgATGTGTTGCTCTATTATCCTTATCACTCATTTGAGCATGTGCTGGAACTGCTGCGTAAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACGGCGTGGCGAAATATTCACGCATCATCGACTCGATGATCCAcg                                                                                                 <  2:4631982/150‑1 (MQ=255)
                                                    gcgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcg                                                                                                 <  1:1165882/150‑1 (MQ=255)
                                                     cgATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcgc                                                                                                >  1:1437337/1‑150 (MQ=255)
                                                            ttGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCCTCGACTCGATGATCCACGCCGCACAt                                                                                         <  2:3525897/150‑1 (MQ=255)
                                                            ttGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt                                                                                         <  2:4246690/150‑1 (MQ=255)
                                                                tctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACg                                                                                     <  1:1547295/150‑1 (MQ=255)
                                                                tctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATGAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACg                                                                                     >  1:304014/1‑150 (MQ=255)
                                                                 ctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACgg                                                                                    >  2:1217976/1‑150 (MQ=255)
                                                                         cTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAg                                                                            <  1:488221/150‑1 (MQ=255)
                                                                         cTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAg                                                                            <  2:1560115/150‑1 (MQ=255)
                                                                          ttATCACTCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGTCTCGATGATCCACGCCGCACATAACGGTAAGAAAGt                                                                           <  2:1097827/150‑1 (MQ=255)
                                                                          ttATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGt                                                                           <  1:742788/150‑1 (MQ=255)
                                                                              cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAcc                                                                       >  1:4636856/1‑150 (MQ=255)
                                                                                cacCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt                                                                     <  1:199544/150‑1 (MQ=255)
                                                                                  ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATGAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg                                                                   >  2:1770405/1‑150 (MQ=255)
                                                                                   cTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                  <  2:3050044/150‑1 (MQ=255)
                                                                                   cTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                  <  1:4030451/150‑1 (MQ=255)
                                                                                    tttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggtg                                                                 <  1:2720715/150‑1 (MQ=255)
                                                                                     ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGtggtgg                                                                <  1:2244737/150‑1 (MQ=255)
                                                                                       gAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt                                                              >  1:1902650/1‑150 (MQ=255)
                                                                                       gAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGGGGTGGtt                                                              >  2:783974/1‑150 (MQ=255)
                                                                                         gCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGa                                                            <  1:775258/150‑1 (MQ=255)
                                                                                          cATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg                                                           <  2:4402966/150‑1 (MQ=255)
                                                                                          cATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg                                                           >  2:2353809/1‑150 (MQ=255)
                                                                                          cATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAg                                                           >  2:2353826/1‑150 (MQ=255)
                                                                                                cTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAg                                                     <  2:3651257/150‑1 (MQ=255)
                                                                                                 tGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCCTCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg                                                    <  1:3108129/150‑1 (MQ=255)
                                                                                                 tGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg                                                    >  2:2179846/1‑150 (MQ=255)
                                                                                                   gAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg                                                  <  2:2935245/150‑1 (MQ=255)
                                                                                                    aaCTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcgc                                                 <  1:4434302/150‑1 (MQ=255)
                                                                                                      ctgctgCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGt                                               <  1:4563583/150‑1 (MQ=255)
                                                                                                       tgctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt                                              <  1:1319627/150‑1 (MQ=255)
                                                                                                       tgctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt                                              <  1:2456899/150‑1 (MQ=255)
                                                                                                        gctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGttt                                             <  2:1403346/150‑1 (MQ=255)
                                                                                                         ctgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc                                            <  2:4109771/150‑1 (MQ=255)
                                                                                                          tgcGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcg                                           <  1:2018719/150‑1 (MQ=255)
                                                                                                               cAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaa                                      >  2:4215080/1‑150 (MQ=255)
                                                                                                                aGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaag                                     >  2:1844265/1‑150 (MQ=255)
                                                                                                                  gCTTCGTTCGACCCGAGCGTACTGGCGATTAACATTAACATTTACCGCGTGGCGAAAGATTCACGAATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACgaagaa                                   <  1:3736783/150‑1 (MQ=255)
                                                                                                                      cgttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCTCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCa                               <  2:4325764/150‑1 (MQ=255)
                                                                                                                        ttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAAc                             >  1:4566164/1‑150 (MQ=255)
                                                                                                                            aCCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACACATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTc                         <  2:633138/150‑1 (MQ=255)
                                                                                                                               cGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACt                      >  2:2563620/1‑150 (MQ=255)
                                                                                                                                gAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTg                     <  2:4363237/150‑1 (MQ=255)
                                                                                                                                gAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTg                     >  1:1751217/1‑150 (MQ=255)
                                                                                                                                       cTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAg              >  2:758672/1‑150 (MQ=255)
                                                                                                                                        tGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAgc             >  2:1733329/1‑150 (MQ=255)
                                                                                                                                           cGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGcc          >  2:204869/1‑150 (MQ=255)
                                                                                                                                           cGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGcc          <  2:658859/150‑1 (MQ=255)
                                                                                                                                           cGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGcc          >  2:799838/1‑150 (MQ=255)
                                                                                                                                            gATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCt         <  1:4076770/150‑1 (MQ=255)
                                                                                                                                            gATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCt         <  1:4093313/150‑1 (MQ=255)
                                                                                                                                             aTTAAAATGAACATGTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTg        <  2:1304399/150‑1 (MQ=255)
                                                                                                                                              ttAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGa       <  1:4402095/150‑1 (MQ=255)
                                                                                                                                               tAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGAc      >  2:1344102/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAA  >  CP009273/2617366‑2617662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A