Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,683,829	C→A	100%	R210L (CGT→CTT)	glrK ←	sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,683,829	0	C	A	98.2%	770.7 / 4.5	235	R210L (CGT→CTT)	glrK	sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
Reads supporting (aligned to +/- strand): ref base C (5/0); new base A (111/119); total (116/119)
Fisher's exact test for biased strand distribution p-value = 2.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG  >  CP009273/2683682‑2683974

tGAGATAAATGTATTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAgg                                                                                                                                                 <  1:777528/150‑1 (MQ=255)

  agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGtt                                                                                                                                               <  1:3288008/150‑1 (MQ=255)

     tAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGAt                                                                                                                                            <  1:1000584/150‑1 (MQ=255)

     tAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGAt                                                                                                                                            >  2:3525901/1‑150 (MQ=255)

      aaaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATc                                                                                                                                           <  2:2932183/150‑1 (MQ=255)

         tGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATg                                                                                                                                        <  1:3413659/150‑1 (MQ=255)

              tAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTc                                                                                                                                   <  1:42127/150‑1 (MQ=255)

              tAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTc                                                                                                                                   <  1:1119365/150‑1 (MQ=255)

                aaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGa                                                                                                                                 <  1:3551379/150‑1 (MQ=255)

                 aaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGat                                                                                                                                <  1:2804893/150‑1 (MQ=255)

                 aaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGat                                                                                                                                <  2:873748/150‑1 (MQ=255)

                      gATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAtttt                                                                                                                           >  1:3722483/1‑150 (MQ=255)

                      gATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAtttt                                                                                                                           <  2:698407/150‑1 (MQ=255)

                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         <  1:1692482/150‑1 (MQ=255)

                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         >  2:4558105/1‑150 (MQ=255)

                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         <  1:1988263/150‑1 (MQ=255)

                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         <  2:2930170/150‑1 (MQ=255)

                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         >  2:1132265/1‑150 (MQ=255)

                           cGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCAcc                                                                                                                      >  1:1469883/1‑150 (MQ=255)

                           cGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCAcc                                                                                                                      <  1:937435/150‑1 (MQ=255)

                            gTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCg                                                                                                                     >  1:4509610/1‑150 (MQ=255)

                            gTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCg                                                                                                                     <  2:2690089/150‑1 (MQ=255)

                             ttGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCgg                                                                                                                    >  1:187471/1‑150 (MQ=255)

                                ggATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGccc                                                                                                                 <  1:4297734/150‑1 (MQ=255)

                                  aTTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGa                                                                                                               <  1:4095597/150‑1 (MQ=255)

                                  aTTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGa                                                                                                               <  2:1464876/150‑1 (MQ=255)

                                  aTTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGa                                                                                                               >  2:2130923/1‑150 (MQ=255)

                                  aTTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGAGATTTTTCACCGGCCCGa                                                                                                               >  2:3875465/1‑150 (MQ=255)

                                   ttCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGAt                                                                                                              <  2:3248988/150‑1 (MQ=255)

                                    tCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATa                                                                                                             >  1:4027437/1‑150 (MQ=255)

                                     ccAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATaa                                                                                                            <  2:1412960/150‑1 (MQ=255)

                                      cAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAat                                                                                                           <  1:367689/150‑1 (MQ=255)

                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          <  1:999835/150‑1 (MQ=255)

                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          >  2:275367/1‑150 (MQ=255)

                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          >  2:419844/1‑150 (MQ=255)

                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          <  1:1035694/150‑1 (MQ=255)

                                           aTGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATcc                                                                                                      <  1:2531739/150‑1 (MQ=255)

                                           aTGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATcc                                                                                                      >  1:1827412/1‑150 (MQ=255)

                                           aTGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATcc                                                                                                      >  2:3699743/1‑150 (MQ=255)

                                           aTGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATcc                                                                                                      <  1:2671201/150‑1 (MQ=255)

                                            tGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCcg                                                                                                     <  1:4574888/150‑1 (MQ=255)

                                            tGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCcg                                                                                                     >  2:986321/1‑150 (MQ=255)

                                              aCAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCcgcg                                                                                                   >  2:2528973/1‑150 (MQ=255)

                                                    gcTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGaaaa                                                                                             >  1:511736/1‑150 (MQ=255)

                                                     cTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAg                                                                                            >  1:1976894/1‑150 (MQ=255)

                                                      tCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGc                                                                                           >  2:2210716/1‑150 (MQ=255)

                                                       cACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCa                                                                                          >  1:2563259/1‑150 (MQ=255)

                                                          ttAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCTCCGGCCCGATAATCATCCGCGTGAAAAGCAGTa                                                                                       <  1:2870936/150‑1 (MQ=255)

                                                          ttAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTa                                                                                       >  1:4472405/1‑150 (MQ=255)

                                                          ttAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTa                                                                                       >  2:3973422/1‑150 (MQ=255)

                                                               cAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCAtt                                                                                  <  1:2589573/150‑1 (MQ=255)

                                                                   gAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCa                                                                              <  1:3802121/150‑1 (MQ=255)

                                                                   gAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCa                                                                              >  2:2059434/1‑150 (MQ=255)

                                                                    aaTACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAg                                                                             <  1:2359403/150‑1 (MQ=255)

                                                                       aCGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACt                                                                          <  1:3205219/150‑1 (MQ=255)

                                                                        cGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTc                                                                         <  2:1963616/150‑1 (MQ=255)

                                                                             cccAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAg                                                                    <  1:672590/150‑1 (MQ=255)

                                                                              ccAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGa                                                                   >  2:584230/1‑150 (MQ=255)

                                                                              ccAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGa                                                                   >  1:3072446/1‑150 (MQ=255)

                                                                                aaCCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGaaa                                                                 >  1:1221802/1‑150 (MQ=255)

                                                                                        gTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCa                                                         <  1:2940648/150‑1 (MQ=255)

                                                                                        gTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCa                                                         >  2:2262348/1‑150 (MQ=255)

                                                                                         tAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAg                                                        <  1:901852/150‑1 (MQ=255)

                                                                                          aaCTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGc                                                       >  2:4010425/1‑150 (MQ=255)

                                                                                          aaCTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGc                                                       <  1:4051055/150‑1 (MQ=255)

                                                                                               gctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTg                                                  <  1:1359163/150‑1 (MQ=255)

                                                                                                ctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGc                                                 >  1:159186/1‑150 (MQ=255)

                                                                                                 tcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGcc                                                <  1:2672943/150‑1 (MQ=255)

                                                                                                   ggTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaa                                              <  1:163165/150‑1 (MQ=255)

                                                                                                   ggTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaa                                              <  1:1210352/150‑1 (MQ=255)

                                                                                                   ggTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaa                                              <  1:1410088/150‑1 (MQ=255)

                                                                                                    gTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCATACTCACCAGAAATAGCACCAGCGATTGccaac                                             <  2:4280383/150‑1 (MQ=255)

                                                                                                      ccACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaacca                                           >  2:3615851/1‑150 (MQ=255)

                                                                                                      ccACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaacca                                           <  1:481412/150‑1 (MQ=255)

                                                                                                        aCTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCaaa                                         <  1:4174109/150‑1 (MQ=255)

                                                                                                           gAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAata                                      >  2:4347767/1‑150 (MQ=255)

                                                                                                            aaCGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAatat                                     <  2:2401913/150‑1 (MQ=255)

                                                                                                            aaCGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAatat                                     <  2:3703813/150‑1 (MQ=255)

                                                                                                             aCGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATAtt                                    <  1:1995409/150‑1 (MQ=255)

                                                                                                             aCGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATAtt                                    >  1:1271715/1‑150 (MQ=255)

                                                                                                               gagaCGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGc                                  >  2:1853438/1‑150 (MQ=255)

                                                                                                               gagaCGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGc                                  >  1:3922771/1‑150 (MQ=255)

                                                                                                                agaCGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGcc                                 <  1:160578/150‑1 (MQ=255)

                                                                                                                   cGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCAc                              <  2:2494377/150‑1 (MQ=255)

                                                                                                                    gCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACg                             >  2:3745043/1‑150 (MQ=255)

                                                                                                                    gCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACg                             >  2:532378/1‑150 (MQ=255)

                                                                                                                       aTTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATGGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTc                          <  1:2119126/150‑1 (MQ=255)

                                                                                                                         tGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCgg                        >  2:759161/1‑150 (MQ=255)

                                                                                                                          gCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGc                       <  2:523788/150‑1 (MQ=255)

                                                                                                                           cccAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCg                      >  2:1646044/1‑150 (MQ=255)

                                                                                                                             cAGAGAACGCCCTTCCCCCAAAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGGTCGGCGAt                    >  2:1555419/1‑150 (MQ=255)

                                                                                                                              agagAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAtt                   >  2:2651346/1‑150 (MQ=255)

                                                                                                                              agagAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAtt                   <  2:4131255/150‑1 (MQ=255)

                                                                                                                                agaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTc                 >  2:1377460/1‑150 (MQ=255)

                                                                                                                                 gaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCa                <  2:4590712/150‑1 (MQ=255)

                                                                                                                                    cGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGc             >  1:890091/1‑150 (MQ=255)

                                                                                                                                     gCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCt            >  2:496756/1‑150 (MQ=255)

                                                                                                                                     gCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCt            <  1:275367/150‑1 (MQ=255)

                                                                                                                                       ccTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGa          <  1:306435/150‑1 (MQ=255)

                                                                                                                                             cccAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAgctgc    >  1:3269348/1‑150 (MQ=255)

TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG  >  CP009273/2683682‑2683974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCC  >  CP009273/2683682‑2683977

TGAGATAAATGTATTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGG                                                                                                                                                    <  SRR19973072.779177/150‑1 (MQ=60)

  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                  >  SRR19973072.3070500/1‑150 (MQ=60)

  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                  <  SRR19973072.3294987/150‑1 (MQ=60)

  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                  >  SRR19973072.3070491/1‑150 (MQ=60)

    ATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGA                                                                                                                                                >  SRR19973072.1544078/1‑150 (MQ=60)

     TAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGAT                                                                                                                                               <  SRR19973072.1002774/150‑1 (MQ=60)

     TAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGAT                                                                                                                                               <  SRR19973072.1002850/150‑1 (MQ=60)

      AAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATC                                                                                                                                              >  SRR19973072.4600351/1‑150 (MQ=60)

      AAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATC                                                                                                                                              <  SRR19973072.4533792/150‑1 (MQ=60)

         TGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATG                                                                                                                                           <  SRR19973072.823112/150‑1 (MQ=60)

         TGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATG                                                                                                                                           <  SRR19973072.3420857/150‑1 (MQ=60)

          GTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGC                                                                                                                                          <  SRR19973072.3752409/150‑1 (MQ=60)

             TTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCT                                                                                                                                       <  SRR19973072.4142569/150‑1 (MQ=60)

              TAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTC                                                                                                                                      <  SRR19973072.1121725/150‑1 (MQ=60)

              TAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTC                                                                                                                                      <  SRR19973072.42247/150‑1 (MQ=60)

              TAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTC                                                                                                                                      >  SRR19973072.3277844/1‑150 (MQ=60)

                AAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGA                                                                                                                                    <  SRR19973072.3558839/150‑1 (MQ=60)

                 AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGAT                                                                                                                                   <  SRR19973072.2810897/150‑1 (MQ=60)

                   ATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAT                                                                                                                                 <  SRR19973072.3780468/150‑1 (MQ=60)

                   ATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAT                                                                                                                                 <  SRR19973072.1079483/150‑1 (MQ=60)

                      GATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTT                                                                                                                              >  SRR19973072.3730319/1‑150 (MQ=60)

                       ATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTT                                                                                                                             <  SRR19973072.2941736/150‑1 (MQ=60)

                        TGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTC                                                                                                                            <  SRR19973072.1992631/150‑1 (MQ=60)

                        TGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTC                                                                                                                            <  SRR19973072.1992540/150‑1 (MQ=60)

                        TGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTC                                                                                                                            <  SRR19973072.1696182/150‑1 (MQ=60)

                        TGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTC                                                                                                                            <  SRR19973072.1696103/150‑1 (MQ=60)

                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                         <  SRR19973072.939485/150‑1 (MQ=60)

                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                         <  SRR19973072.939484/150‑1 (MQ=60)

                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                         <  SRR19973072.1874434/150‑1 (MQ=60)

                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                         >  SRR19973072.1473035/1‑150 (MQ=60)

                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                        <  SRR19973072.1906183/150‑1 (MQ=60)

                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                        <  SRR19973072.1906593/150‑1 (MQ=60)

                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                        >  SRR19973072.4519131/1‑150 (MQ=60)

                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                        >  SRR19973072.4518605/1‑150 (MQ=60)

                             TTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGG                                                                                                                       >  SRR19973072.187949/1‑150 (MQ=60)

                              TGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGC                                                                                                                      <  SRR19973072.2568608/150‑1 (MQ=60)

                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                    <  SRR19973072.1766974/150‑1 (MQ=60)

                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                    >  SRR19973072.2301414/1‑150 (MQ=60)

                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                    <  SRR19973072.4306694/150‑1 (MQ=60)

                                  ATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGA                                                                                                                  <  SRR19973072.4104198/150‑1 (MQ=60)

                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                <  SRR19973072.1633083/150‑1 (MQ=60)

                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                <  SRR19973072.3834672/150‑1 (MQ=60)

                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                >  SRR19973072.3143433/1‑150 (MQ=60)

                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                >  SRR19973072.4035895/1‑150 (MQ=60)

                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                >  SRR19973072.4035900/1‑150 (MQ=60)

                                      CAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAAT                                                                                                              >  SRR19973072.4420245/1‑150 (MQ=60)

                                      CAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAAT                                                                                                              <  SRR19973072.368497/150‑1 (MQ=60)

                                      CAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAAT                                                                                                              >  SRR19973072.2413385/1‑150 (MQ=60)

                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                             <  SRR19973072.1002022/150‑1 (MQ=60)

                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                             <  SRR19973072.1037933/150‑1 (MQ=60)

                                           ATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCC                                                                                                         <  SRR19973072.2537239/150‑1 (MQ=60)

                                           ATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCC                                                                                                         >  SRR19973072.1831394/1‑150 (MQ=60)

                                           ATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCC                                                                                                         <  SRR19973072.2076793/150‑1 (MQ=60)

                                           ATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCC                                                                                                         <  SRR19973072.3575120/150‑1 (MQ=60)

                                           ATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCC                                                                                                         <  SRR19973072.2676906/150‑1 (MQ=60)

                                            TGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCG                                                                                                        <  SRR19973072.4584512/150‑1 (MQ=60)

                                            TGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCG                                                                                                        >  SRR19973072.923851/1‑150 (MQ=60)

                                            TGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCG                                                                                                        >  SRR19973072.923894/1‑150 (MQ=60)

                                                 GGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGA                                                                                                   <  SRR19973072.1595827/150‑1 (MQ=60)

                                                    GCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAA                                                                                                >  SRR19973072.2716480/1‑150 (MQ=60)

                                                    GCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAA                                                                                                >  SRR19973072.512956/1‑150 (MQ=60)

                                                     CTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAG                                                                                               >  SRR19973072.344224/1‑150 (MQ=60)

                                                     CTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAG                                                                                               >  SRR19973072.1981132/1‑150 (MQ=60)

                                                     CTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAG                                                                                               >  SRR19973072.1981350/1‑150 (MQ=60)

                                                      TCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGC                                                                                              >  SRR19973072.507753/1‑150 (MQ=60)

                                                       CACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCA                                                                                             >  SRR19973072.2568810/1‑150 (MQ=60)

                                                          TTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCTCCGGCCCGATAATCATCCGCGTGAAAAGCAGTA                                                                                          <  SRR19973072.2877087/150‑1 (MQ=60)

                                                          TTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTA                                                                                          >  SRR19973072.4481848/1‑150 (MQ=60)

                                                           TAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTAC                                                                                         >  SRR19973072.4238147/1‑150 (MQ=60)

                                                               CAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATT                                                                                     <  SRR19973072.2595163/150‑1 (MQ=60)

                                                               CAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATT                                                                                     <  SRR19973072.2595162/150‑1 (MQ=60)

                                                                AAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTA                                                                                    <  SRR19973072.764700/150‑1 (MQ=60)

                                                                AAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTA                                                                                    <  SRR19973072.1321402/150‑1 (MQ=60)

                                                                   GAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCA                                                                                 <  SRR19973072.3810082/150‑1 (MQ=60)

                                                                    AATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAG                                                                                <  SRR19973072.2364671/150‑1 (MQ=60)

                                                                    AATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAG                                                                                <  SRR19973072.2364484/150‑1 (MQ=60)

                                                                       ACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACT                                                                             <  SRR19973072.3211994/150‑1 (MQ=60)

                                                                        CGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTC                                                                            <  SRR19973072.3529168/150‑1 (MQ=60)

                                                                             CCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAG                                                                       <  SRR19973072.674090/150‑1 (MQ=60)

                                                                             CCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAG                                                                       <  SRR19973072.674088/150‑1 (MQ=60)

                                                                              CCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGA                                                                      >  SRR19973072.754472/1‑150 (MQ=60)

                                                                              CCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGA                                                                      >  SRR19973072.3078938/1‑150 (MQ=60)

                                                                                AACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAA                                                                    >  SRR19973072.1224409/1‑150 (MQ=60)

                                                                                AACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAA                                                                    >  SRR19973072.1224410/1‑150 (MQ=60)

                                                                                AACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAA                                                                    >  SRR19973072.1224391/1‑150 (MQ=60)

                                                                                    GAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGC                                                                <  SRR19973072.2819487/150‑1 (MQ=60)

                                                                                    GAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGC                                                                <  SRR19973072.3689409/150‑1 (MQ=60)

                                                                                       CGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACC                                                             <  SRR19973072.4213177/150‑1 (MQ=60)

                                                                                        GTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCA                                                            <  SRR19973072.2946937/150‑1 (MQ=60)

                                                                                        GTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCA                                                            >  SRR19973072.1137509/1‑150 (MQ=60)

                                                                                         TAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAG                                                           <  SRR19973072.903802/150‑1 (MQ=60)

                                                                                         TAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAG                                                           <  SRR19973072.903717/150‑1 (MQ=60)

                                                                                          AACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGC                                                          <  SRR19973072.4059557/150‑1 (MQ=60)

                                                                                          AACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGC                                                          >  SRR19973072.4077799/1‑150 (MQ=60)

                                                                                              CGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATT                                                      <  SRR19973072.1150229/150‑1 (MQ=60)

                                                                                               GCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTG                                                     <  SRR19973072.1361267/150‑1 (MQ=60)

                                                                                               GCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTG                                                     <  SRR19973072.1362140/150‑1 (MQ=60)

                                                                                                CTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGC                                                    >  SRR19973072.159590/1‑150 (MQ=60)

                                                                                                CTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGC                                                    >  SRR19973072.4046712/1‑150 (MQ=60)

                                                                                                 TCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCC                                                   <  SRR19973072.2678648/150‑1 (MQ=60)

                                                                                                 TCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCC                                                   <  SRR19973072.2678624/150‑1 (MQ=60)

                                                                                                  CGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCA                                                  >  SRR19973072.1587957/1‑150 (MQ=60)

                                                                                                   GGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAA                                                 <  SRR19973072.1413154/150‑1 (MQ=60)

                                                                                                   GGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAA                                                 <  SRR19973072.1212943/150‑1 (MQ=60)

                                                                                                   GGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAA                                                 <  SRR19973072.1413158/150‑1 (MQ=60)

                                                                                                   GGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAA                                                 <  SRR19973072.163581/150‑1 (MQ=60)

                                                                                                      CCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCA                                              <  SRR19973072.482543/150‑1 (MQ=60)

                                                                                                      CCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCA                                              >  SRR19973072.3394054/1‑150 (MQ=60)

                                                                                                      CCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCA                                              <  SRR19973072.4474281/150‑1 (MQ=60)

                                                                                                        ACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAA                                            <  SRR19973072.4182845/150‑1 (MQ=60)

                                                                                                        ACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAA                                            <  SRR19973072.3265523/150‑1 (MQ=60)

                                                                                                          TGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAAT                                          <  SRR19973072.4113210/150‑1 (MQ=60)

                                                                                                             ACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATT                                       <  SRR19973072.1999706/150‑1 (MQ=60)

                                                                                                             ACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATT                                       >  SRR19973072.1274459/1‑150 (MQ=60)

                                                                                                               GAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGC                                     >  SRR19973072.3930960/1‑150 (MQ=60)

                                                                                                                AGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCC                                    <  SRR19973072.160987/150‑1 (MQ=60)

                                                                                                                AGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCC                                    <  SRR19973072.2083952/150‑1 (MQ=60)

                                                                                                                       ATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATGGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTC                             <  SRR19973072.2123747/150‑1 (MQ=60)

                                                                                                                        TTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCG                            >  SRR19973072.472158/1‑150 (MQ=60)

                                                                                                                              AGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATT                      >  SRR19973072.2802480/1‑150 (MQ=60)

                                                                                                                              AGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATT                      >  SRR19973072.2802655/1‑150 (MQ=60)

                                                                                                                                    CGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGC                >  SRR19973072.892012/1‑150 (MQ=60)

                                                                                                                                    CGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGC                >  SRR19973072.4009100/1‑150 (MQ=60)

                                                                                                                                    CGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGC                >  SRR19973072.1680980/1‑150 (MQ=60)

                                                                                                                                     GCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCT               <  SRR19973072.276014/150‑1 (MQ=60)

                                                                                                                                       CCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGA             <  SRR19973072.307144/150‑1 (MQ=60)

                                                                                                                                        CTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAA            >  SRR19973072.3071826/1‑150 (MQ=60)

                                                                                                                                             CCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGC       >  SRR19973072.3276260/1‑150 (MQ=60)

                                                                                                                                             CCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGC       >  SRR19973072.3276290/1‑150 (MQ=60)

                                                                                                                                               CAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG     >  SRR19973072.2639940/1‑150 (MQ=60)

                                                                                                                                               CAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG     >  SRR19973072.2639933/1‑150 (MQ=60)

                                                                                                                                               CAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG     >  SRR19973072.1444697/1‑150 (MQ=60)

                                                                                                                                                 GAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCC   <  SRR19973072.3441938/150‑1 (MQ=60)

                                                                                                                                                 GAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCC   <  SRR19973072.3441988/150‑1 (MQ=60)

                                                                                                                                                  AAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCC  >  SRR19973072.1949189/1‑150 (MQ=60)

                                                                                                                                                  AAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCC  >  SRR19973072.1654379/1‑150 (MQ=60)

TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCC  >  CP009273/2683682‑2683977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`