Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,683,829 C→A 91.5% R210L (CGT→CTT)  glrK ← sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,683,8290CA91.5% 657.2 / 50.4 223R210L (CGT→CTT) glrKsensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
Reads supporting (aligned to +/- strand):  ref base C (7/12);  new base A (103/101);  total (110/113)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 223 total aligned reads displayed.

TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG  >  CP009273/2683682‑2683974
                                                                                                                                                   |                                                                                                                                                 
  ataTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGtt                                                                                                                                               >  1:3759857/3‑150 (MQ=255)
  agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGtt                                                                                                                                               >  1:847073/1‑150 (MQ=255)
   gaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTg                                                                                                                                              <  2:1088091/150‑1 (MQ=255)
      aaaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATc                                                                                                                                           <  2:3346842/150‑1 (MQ=255)
        aTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCAt                                                                                                                                         <  2:907831/150‑1 (MQ=255)
         tGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATg                                                                                                                                        <  1:2840393/150‑1 (MQ=255)
          gTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATgc                                                                                                                                       >  1:3888301/1‑150 (MQ=255)
          gTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATgc                                                                                                                                       >  1:3485994/1‑150 (MQ=255)
           tCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATgcg                                                                                                                                      <  2:890443/150‑1 (MQ=255)
             ttAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCt                                                                                                                                    <  2:1396949/150‑1 (MQ=255)
              tAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTc                                                                                                                                   <  2:189305/150‑1 (MQ=255)
              tAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTc                                                                                                                                   <  2:187730/150‑1 (MQ=255)
               aaaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCg                                                                                                                                  <  1:1876799/150‑1 (MQ=255)
               aaaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCg                                                                                                                                  >  2:2653991/1‑150 (MQ=255)
               aaaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCg                                                                                                                                  <  1:4223317/150‑1 (MQ=255)
               aaaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCg                                                                                                                                  >  1:2518539/1‑150 (MQ=255)
                aaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGa                                                                                                                                 >  1:73704/1‑150 (MQ=255)
                aaaaTTGAAGGCGTTGGGATTCCAGCCATTACAGTCGATCACTTAACCAAAGAATACGTTGCCCAAAAGAGCGTAACTCTCTCGGTCCACTGAACGAGAAGCTATTGCCCAGAGAAATCCCTTACACCAGAATGTTGATCATGAGCTCGa                                                                                                                                 >  2:1951787/1‑150 (MQ=255)
                 aaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGat                                                                                                                                >  1:2011630/1‑150 (MQ=255)
                  aaTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGata                                                                                                                               >  2:2517739/1‑150 (MQ=255)
                   aTTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGatat                                                                                                                              >  1:1141498/1‑150 (MQ=255)
                    ttGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAtt                                                                                                                             <  1:728071/150‑1 (MQ=255)
                     tGATGGCGTTGGGATTCCAGCCCTGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCCGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATAttt                                                                                                                            >  2:630205/1‑150 (MQ=255)
                        tGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTc                                                                                                                         <  2:4192894/150‑1 (MQ=255)
                           cGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCAcc                                                                                                                      <  1:1055325/150‑1 (MQ=255)
                           cGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCAcc                                                                                                                      <  1:3249650/150‑1 (MQ=255)
                           cGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCAcc                                                                                                                      >  2:2103535/1‑150 (MQ=255)
                            gTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCg                                                                                                                     >  1:4135970/1‑150 (MQ=255)
                              tGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGc                                                                                                                   <  2:2331915/150‑1 (MQ=255)
                               gggATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGcc                                                                                                                  >  1:2848913/1‑150 (MQ=255)
                                ggATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGccc                                                                                                                 >  1:958946/1‑150 (MQ=255)
                                ggATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGccc                                                                                                                 >  1:231482/1‑150 (MQ=255)
                                 gATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCg                                                                                                                >  2:2975167/1‑150 (MQ=255)
                                    tCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATa                                                                                                             <  1:2713958/150‑1 (MQ=255)
                                     ccAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATaa                                                                                                            >  2:2825320/1‑150 (MQ=255)
                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          >  1:2084411/1‑150 (MQ=255)
                                       aGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatc                                                                                                          <  1:513611/150‑1 (MQ=255)
                                         ccATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatcat                                                                                                        <  2:3518097/150‑1 (MQ=255)
                                          cATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAatcatc                                                                                                       <  1:2128773/150‑1 (MQ=255)
                                                aGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTg                                                                                                 >  2:1760433/1‑150 (MQ=255)
                                                aGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTg                                                                                                 <  2:886215/150‑1 (MQ=255)
                                                 ggCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGa                                                                                                <  1:1433861/150‑1 (MQ=255)
                                                    gcTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGaaaa                                                                                             <  2:920708/150‑1 (MQ=255)
                                                       cACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCa                                                                                          >  1:416319/1‑150 (MQ=255)
                                                        aCTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAg                                                                                         >  2:1210059/1‑150 (MQ=255)
                                                            aaCCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTAcc                                                                                     >  2:433281/1‑150 (MQ=255)
                                                            aaCCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTAcc                                                                                     <  1:1788772/150‑1 (MQ=255)
                                                              ccAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCAt                                                                                   <  2:4048119/150‑1 (MQ=255)
                                                              ccAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCAt                                                                                   <  2:2174160/150‑1 (MQ=255)
                                                               cAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCAtt                                                                                  <  1:1582338/150‑1 (MQ=255)
                                                                 aaGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTAc                                                                                >  2:1462790/1‑150 (MQ=255)
                                                                 aaGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTAc                                                                                >  1:2663416/1‑150 (MQ=255)
                                                                    aaTTCGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAg                                                                             >  2:3604679/1‑150 (MQ=255)
                                                                     aTACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGa                                                                            >  1:2405358/1‑150 (MQ=255)
                                                                       aCGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACt                                                                          >  1:259006/1‑150 (MQ=255)
                                                                        cGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTc                                                                         >  1:292914/1‑150 (MQ=255)
                                                                         gTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCa                                                                        <  1:2810429/150‑1 (MQ=255)
                                                                            gCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGATAAGCAGTACCATTACCAGACTCACCa                                                                     <  2:2466998/150‑1 (MQ=255)
                                                                             cccAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAg                                                                    >  1:3116779/1‑150 (MQ=255)
                                                                                aaCCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGaaa                                                                 >  2:3875893/1‑150 (MQ=255)
                                                                                 aCCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAAt                                                                <  2:1528529/150‑1 (MQ=255)
                                                                                  ccGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATa                                                               >  2:3988327/1‑150 (MQ=255)
                                                                                   cGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAg                                                              <  2:4128752/150‑1 (MQ=255)
                                                                                       cGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCAcc                                                          <  2:2111350/150‑1 (MQ=255)
                                                                                       cGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCAcc                                                          >  2:3566377/1‑150 (MQ=255)
                                                                                        gTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCa                                                         >  2:1997131/1‑150 (MQ=255)
                                                                                         tAACTCGCTCGGTCCACTGAACGCGACGCTATTGCCCAGAGACCGCCCTGCCCCCCGAAGGTTGCTCCTGCGCGCGATATTTTTCACCGGCCCGATAATCATCCGCGTGGACAGCCGTACCATTACCGGTCTCACCAGAAAGAGCCCCAg                                                        >  2:3165494/1‑150 (MQ=255)
                                                                                          aaCTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACTAGAAATAGCACCAGc                                                       >  2:1435198/1‑150 (MQ=255)
                                                                                           aCTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCg                                                      >  1:1241271/1‑150 (MQ=255)
                                                                                            ctcgctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGa                                                     <  2:844289/150‑1 (MQ=255)
                                                                                             tcgctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAt                                                    <  1:1514627/150‑1 (MQ=255)
                                                                                              cgctcgGTCCACTGACCGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCGCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCAGTACCAGACTCACCAGAAATAGCACCAGCGAtt                                                   <  2:673282/150‑1 (MQ=255)
                                                                                              cgctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAtt                                                   <  2:3717346/150‑1 (MQ=255)
                                                                                                ctcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGc                                                 >  1:424161/1‑150 (MQ=255)
                                                                                                 tcgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGcc                                                >  1:2178507/1‑150 (MQ=255)
                                                                                                  cgGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGcca                                               >  1:3264592/1‑150 (MQ=255)
                                                                                                    gTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaac                                             >  1:224257/1‑150 (MQ=255)
                                                                                                    gTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaac                                             >  1:224135/1‑150 (MQ=255)
                                                                                                     tCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGccaacc                                            >  1:704826/1‑150 (MQ=255)
                                                                                                         cTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCaaaa                                        >  1:303102/1‑150 (MQ=255)
                                                                                                          tGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAat                                       >  2:1536688/1‑150 (MQ=255)
                                                                                                                 gaCGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGccc                                <  1:3976859/150‑1 (MQ=255)
                                                                                                                  aCGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATACTCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCa                               >  2:3185787/1‑150 (MQ=255)
                                                                                                                       aTTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCCGACTCACCAGAAATAGCACCAGCGATTGCCAACCACAATCTTGCCCACGTTc                          >  2:2992587/1‑150 (MQ=255)
                                                                                                                        ttGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCg                         <  1:3138068/150‑1 (MQ=255)
                                                                                                                         tGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCgg                        <  2:167795/150‑1 (MQ=255)
                                                                                                                         tGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCgg                        <  2:732964/150‑1 (MQ=255)
                                                                                                                         tGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCgg                        <  1:281962/150‑1 (MQ=255)
                                                                                                                          gCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGc                       <  1:2766609/150‑1 (MQ=255)
                                                                                                                           cccAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCg                      <  2:1277721/150‑1 (MQ=255)
                                                                                                                            ccAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGa                     <  2:1876520/150‑1 (MQ=255)
                                                                                                                             cAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAt                    >  1:3616457/1‑150 (MQ=255)
                                                                                                                               gagaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAttt                  <  1:600134/150‑1 (MQ=255)
                                                                                                                                agaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTc                 >  2:2663812/1‑150 (MQ=255)
                                                                                                                                agaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTc                 >  2:1709861/1‑150 (MQ=255)
                                                                                                                                 gaACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCa                <  1:1989158/150‑1 (MQ=255)
                                                                                                                                   aCGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACg              <  1:2210641/150‑1 (MQ=255)
                                                                                                                                     gCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCt            >  1:1193517/1‑150 (MQ=255)
                                                                                                                                           cccccAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAgct      <  2:3890122/150‑1 (MQ=255)
                                                                                                                                           cccccAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAgct      <  2:3819045/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTG  >  CP009273/2683682‑2683974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCCA  >  CP009273/2683682‑2683978
                                                                                                                                                   |                                                                                                                                                     
TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGG                                                                                                                                                     >  SRR19973079.3343764/1‑150 (MQ=60)
TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGG                                                                                                                                                     >  SRR19973079.548190/1‑150 (MQ=60)
 GAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGT                                                                                                                                                    >  SRR19973079.220382/1‑150 (MQ=60)
  ATATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                   >  SRR19973079.3779124/1‑150 (MQ=60)
  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                   >  SRR19973079.851972/1‑150 (MQ=60)
  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                   >  SRR19973079.851949/1‑150 (MQ=60)
  AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTT                                                                                                                                                   >  SRR19973079.3782715/1‑150 (MQ=60)
       AATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCA                                                                                                                                              <  SRR19973079.4162001/150‑1 (MQ=60)
         TGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATG                                                                                                                                            <  SRR19973079.2854871/150‑1 (MQ=60)
          GTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGC                                                                                                                                           >  SRR19973079.3503940/1‑150 (MQ=60)
          GTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGC                                                                                                                                           >  SRR19973079.3908052/1‑150 (MQ=60)
            CTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGC                                                                                                                                         >  SRR19973079.866767/1‑150 (MQ=60)
               AAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCG                                                                                                                                      <  SRR19973079.1886773/150‑1 (MQ=60)
               AAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCG                                                                                                                                      >  SRR19973079.2531274/1‑150 (MQ=60)
               AAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCG                                                                                                                                      <  SRR19973079.4244689/150‑1 (MQ=60)
                AAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGA                                                                                                                                     >  SRR19973079.74397/1‑150 (MQ=60)
                 AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGAT                                                                                                                                    >  SRR19973079.2021757/1‑150 (MQ=60)
                 AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGAT                                                                                                                                    >  SRR19973079.2022254/1‑150 (MQ=60)
                 AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGAT                                                                                                                                    >  SRR19973079.2022723/1‑150 (MQ=60)
                  AATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATA                                                                                                                                   <  SRR19973079.2859417/150‑1 (MQ=60)
                   ATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATAT                                                                                                                                  >  SRR19973079.1148036/1‑150 (MQ=60)
                    TTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATT                                                                                                                                 <  SRR19973079.731923/150‑1 (MQ=60)
                      GATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTT                                                                                                                               <  SRR19973079.3149650/150‑1 (MQ=60)
                       ATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTT                                                                                                                              <  SRR19973079.1229659/150‑1 (MQ=60)
                       ATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTT                                                                                                                              <  SRR19973079.1925099/150‑1 (MQ=60)
                       ATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTT                                                                                                                              <  SRR19973079.2185049/150‑1 (MQ=60)
                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                          <  SRR19973079.1061119/150‑1 (MQ=60)
                           CGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACC                                                                                                                          <  SRR19973079.3266075/150‑1 (MQ=60)
                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                         >  SRR19973079.4156956/1‑150 (MQ=60)
                            GTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCG                                                                                                                         >  SRR19973079.2305702/1‑150 (MQ=60)
                               GGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCC                                                                                                                      >  SRR19973079.2863416/1‑150 (MQ=60)
                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                     >  SRR19973079.964478/1‑150 (MQ=60)
                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                     >  SRR19973079.964426/1‑150 (MQ=60)
                                GGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCC                                                                                                                     >  SRR19973079.233045/1‑150 (MQ=60)
                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                 <  SRR19973079.2729146/150‑1 (MQ=60)
                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                 <  SRR19973079.2728000/150‑1 (MQ=60)
                                    TCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATA                                                                                                                 <  SRR19973079.2727982/150‑1 (MQ=60)
                                     CCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAA                                                                                                                <  SRR19973079.2968271/150‑1 (MQ=60)
                                      CAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAAT                                                                                                               >  SRR19973079.2608635/1‑150 (MQ=60)
                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                              <  SRR19973079.516715/150‑1 (MQ=60)
                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                              <  SRR19973079.2699408/150‑1 (MQ=60)
                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                              >  SRR19973079.1143696/1‑150 (MQ=60)
                                       AGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATC                                                                                                              >  SRR19973079.2095286/1‑150 (MQ=60)
                                        GCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCA                                                                                                             >  SRR19973079.67738/1‑150 (MQ=60)
                                          CATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATC                                                                                                           <  SRR19973079.2139768/150‑1 (MQ=60)
                                                AGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTG                                                                                                     <  SRR19973079.2385249/150‑1 (MQ=60)
                                                 GGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGA                                                                                                    <  SRR19973079.1441606/150‑1 (MQ=60)
                                                       CACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCA                                                                                              >  SRR19973079.418757/1‑150 (MQ=60)
                                                       CACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGAGAAAAGCA                                                                                              >  SRR19973079.3129247/1‑150 (MQ=60)
                                                            AACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACC                                                                                         <  SRR19973079.1798113/150‑1 (MQ=60)
                                                             ACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCA                                                                                        >  SRR19973079.4088056/1‑150 (MQ=60)
                                                               CAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATT                                                                                      <  SRR19973079.1591072/150‑1 (MQ=60)
                                                               CAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATT                                                                                      <  SRR19973079.1915672/150‑1 (MQ=60)
                                                                 AAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTAC                                                                                    >  SRR19973079.2677214/1‑150 (MQ=60)
                                                                  AGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACC                                                                                   <  SRR19973079.3251752/150‑1 (MQ=60)
                                                                    AATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAG                                                                                 >  SRR19973079.1453474/1‑150 (MQ=60)
                                                                     ATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGA                                                                                >  SRR19973079.2417771/1‑150 (MQ=60)
                                                                     ATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGA                                                                                >  SRR19973079.2419249/1‑150 (MQ=60)
                                                                       ACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACT                                                                              >  SRR19973079.260669/1‑150 (MQ=60)
                                                                        CGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTC                                                                             >  SRR19973079.294675/1‑150 (MQ=60)
                                                                         GTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCA                                                                            <  SRR19973079.2824813/150‑1 (MQ=60)
                                                                          TTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCAC                                                                           <  SRR19973079.1599707/150‑1 (MQ=60)
                                                                             CCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAG                                                                        >  SRR19973079.3132924/1‑150 (MQ=60)
                                                                             CCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAG                                                                        >  SRR19973079.3132848/1‑150 (MQ=60)
                                                                                        GTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCA                                                             <  SRR19973079.2859000/150‑1 (MQ=60)
                                                                                           ACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCG                                                          <  SRR19973079.3565181/150‑1 (MQ=60)
                                                                                           ACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCG                                                          >  SRR19973079.1248392/1‑150 (MQ=60)
                                                                                           ACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCG                                                          <  SRR19973079.3565613/150‑1 (MQ=60)
                                                                                             TCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAT                                                        <  SRR19973079.733244/150‑1 (MQ=60)
                                                                                             TCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAT                                                        <  SRR19973079.1522975/150‑1 (MQ=60)
                                                                                             TCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAT                                                        <  SRR19973079.1523192/150‑1 (MQ=60)
                                                                                             TCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGAT                                                        >  SRR19973079.4193211/1‑150 (MQ=60)
                                                                                                CTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGC                                                     >  SRR19973079.426665/1‑150 (MQ=60)
                                                                                                CTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGC                                                     >  SRR19973079.426653/1‑150 (MQ=60)
                                                                                                 TCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCC                                                    >  SRR19973079.2189644/1‑150 (MQ=60)
                                                                                                  CGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCA                                                   >  SRR19973079.3280892/1‑150 (MQ=60)
                                                                                                  CGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCA                                                   >  SRR19973079.3281069/1‑150 (MQ=60)
                                                                                                    GTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAAC                                                 >  SRR19973079.491347/1‑150 (MQ=60)
                                                                                                    GTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAAC                                                 >  SRR19973079.225794/1‑150 (MQ=60)
                                                                                                    GTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAAC                                                 >  SRR19973079.225674/1‑150 (MQ=60)
                                                                                                    GTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAAC                                                 >  SRR19973079.225672/1‑150 (MQ=60)
                                                                                                     TCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACC                                                >  SRR19973079.708621/1‑150 (MQ=60)
                                                                                                     TCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACC                                                >  SRR19973079.708644/1‑150 (MQ=60)
                                                                                                         CTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAA                                            >  SRR19973079.304888/1‑150 (MQ=60)
                                                                                                           GAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATA                                          <  SRR19973079.824490/150‑1 (MQ=60)
                                                                                                                 GACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCC                                    <  SRR19973079.3996853/150‑1 (MQ=60)
                                                                                                                 GACGCTATTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCC                                    <  SRR19973079.1859867/150‑1 (MQ=60)
                                                                                                                       ATTGCCCAGAGAACGCCCGTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTC                              <  SRR19973079.1470758/150‑1 (MQ=60)
                                                                                                                        TTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCG                             <  SRR19973079.395991/150‑1 (MQ=60)
                                                                                                                        TTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCG                             <  SRR19973079.3691505/150‑1 (MQ=60)
                                                                                                                        TTGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCG                             <  SRR19973079.3154209/150‑1 (MQ=60)
                                                                                                                         TGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGG                            <  SRR19973079.283665/150‑1 (MQ=60)
                                                                                                                         TGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGG                            <  SRR19973079.283703/150‑1 (MQ=60)
                                                                                                                         TGCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGG                            <  SRR19973079.283706/150‑1 (MQ=60)
                                                                                                                          GCCCAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGC                           <  SRR19973079.2780842/150‑1 (MQ=60)
                                                                                                                             CAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAT                        >  SRR19973079.2389182/1‑150 (MQ=60)
                                                                                                                             CAGAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGAT                        >  SRR19973079.3634766/1‑150 (MQ=60)
                                                                                                                               GAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTT                      <  SRR19973079.603625/150‑1 (MQ=60)
                                                                                                                               GAGAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTT                      <  SRR19973079.603629/150‑1 (MQ=60)
                                                                                                                                 GAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCA                    <  SRR19973079.1999711/150‑1 (MQ=60)
                                                                                                                                 GAACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCA                    <  SRR19973079.212107/150‑1 (MQ=60)
                                                                                                                                   ACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACG                  <  SRR19973079.2222052/150‑1 (MQ=60)
                                                                                                                                   ACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACG                  <  SRR19973079.2221971/150‑1 (MQ=60)
                                                                                                                                   ACGCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATAGTGCCCACGTTCGGCGATTTCACG                  <  SRR19973079.2224880/150‑1 (MQ=60)
                                                                                                                                     GCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCT                >  SRR19973079.1266577/1‑150 (MQ=60)
                                                                                                                                     GCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCT                >  SRR19973079.1200387/1‑150 (MQ=60)
                                                                                                                                     GCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCT                >  SRR19973079.1200385/1‑150 (MQ=60)
                                                                                                                                     GCCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACAAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCAACGTTCGGCGATTTCACGCT                >  SRR19973079.4164437/1‑150 (MQ=60)
                                                                                                                                      CCCTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCAGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTG               <  SRR19973079.2824463/150‑1 (MQ=60)
                                                                                                                                        CTTCCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAA             <  SRR19973079.2897356/150‑1 (MQ=60)
                                                                                                                                           CCCCCAGAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCT          >  SRR19973079.253663/1‑150 (MQ=60)
                                                                                                                                                 GAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCC    >  SRR19973079.722868/1‑150 (MQ=60)
                                                                                                                                                 GAAGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCC    >  SRR19973079.723285/1‑150 (MQ=60)
                                                                                                                                                   AGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCCA  >  SRR19973079.1628626/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAGACGGTTGATCATGCGCTCGATATTTTTCACCGGCCCGATAATCATCCGCGTGAAAAGCAGTACCATTACCAGACTCACCAGAAATAGCACCAGCGATTGCCAACCAAAATATTGCCCACGTTCGGCGATTTCACGCTGAAGCTGCTGCCCA  >  CP009273/2683682‑2683978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑