Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,740,948 C→A 18.7% T69T (ACG→ACT)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,740,9480CA18.7% 765.6 / 113.7 256T69T (ACG→ACT) ffhSignal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand):  ref base C (90/118);  new base A (25/23);  total (115/141)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 263 total aligned reads displayed.

TTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTCC  >  CP009273/2740799‑2741097
                                                                                                                                                     |                                                                                                                                                     
  aCCAACGCTGGTTGTTTTACCGGCACTTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGt                                                                                                                                                     >  1:2563480/1‑150 (MQ=255)
    cAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCa                                                                                                                                                   >  2:3904126/1‑150 (MQ=255)
     aaCGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAg                                                                                                                                                  <  1:1097909/150‑1 (MQ=255)
     aaCGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAg                                                                                                                                                  <  1:1293740/150‑1 (MQ=255)
      aCGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCACATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAgg                                                                                                                                                 <  2:2463679/150‑1 (MQ=255)
           ggTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCtt                                                                                                                                            >  2:1016291/1‑150 (MQ=255)
            gttgttTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCtta                                                                                                                                           <  1:3287633/150‑1 (MQ=255)
              tgttTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCttatt                                                                                                                                         <  2:450073/150‑1 (MQ=255)
              tgttTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCttatt                                                                                                                                         <  2:2232812/150‑1 (MQ=255)
               gttTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCttatta                                                                                                                                        <  1:4641902/150‑1 (MQ=255)
                  ttACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACt                                                                                                                                     >  1:4466064/1‑150 (MQ=255)
                   tACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACtt                                                                                                                                    <  2:3408902/150‑1 (MQ=255)
                     ccGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCa                                                                                                                                  <  2:3654383/150‑1 (MQ=255)
                      cGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCAt                                                                                                                                 <  2:650064/150‑1 (MQ=255)
                       ggCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATg                                                                                                                                >  2:3417716/1‑150 (MQ=255)
                       ggCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATg                                                                                                                                >  2:3203180/1‑150 (MQ=255)
                          aCCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCTGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGAcc                                                                                                                             <  2:227854/150‑1 (MQ=255)
                          aCCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGAcc                                                                                                                             >  2:2985882/1‑150 (MQ=255)
                           ccTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCa                                                                                                                            <  2:3036066/150‑1 (MQ=255)
                            cTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTATGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCaa                                                                                                                           >  2:3332486/1‑150 (MQ=255)
                             ttGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAAc                                                                                                                          <  2:3783068/150‑1 (MQ=255)
                              tGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAAcc                                                                                                                         <  1:737622/150‑1 (MQ=255)
                                  ggCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCtt                                                                                                                     <  2:4138071/150‑1 (MQ=255)
                                   gCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCttt                                                                                                                    <  1:3155757/150‑1 (MQ=255)
                                      cGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTtctc                                                                                                                 >  1:1036783/1‑150 (MQ=255)
                                      cGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTtctc                                                                                                                 <  1:3946208/150‑1 (MQ=255)
                                      cGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTtcta                                                                                                                 >  1:516778/1‑149 (MQ=255)
                                       gCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTtctcg                                                                                                                <  1:897832/150‑2 (MQ=255)
                                        ccATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCtt                                                                                                               <  1:3602908/150‑1 (MQ=255)
                                         cATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCttt                                                                                                              <  2:4303400/150‑1 (MQ=255)
                                          aTCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCtttt                                                                                                             <  1:3699565/150‑1 (MQ=255)
                                            cAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTAc                                                                                                           <  2:2938023/150‑1 (MQ=255)
                                            cAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTAc                                                                                                           >  1:2101411/1‑150 (MQ=255)
                                            cAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTAc                                                                                                           <  1:4526816/150‑1 (MQ=255)
                                             aGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTAcg                                                                                                          <  2:1363676/150‑1 (MQ=255)
                                             aGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTAcg                                                                                                          >  1:859307/1‑150 (MQ=255)
                                                 cGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGAt                                                                                                      <  1:2697242/150‑1 (MQ=255)
                                                  gACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGAtt                                                                                                     <  1:2979206/150‑1 (MQ=255)
                                                    ccGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGa                                                                                                   <  2:3497799/150‑1 (MQ=255)
                                                      gCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATa                                                                                                 >  1:1428209/1‑150 (MQ=255)
                                                       cAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATaa                                                                                                <  1:1379300/150‑1 (MQ=255)
                                                       cAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATaa                                                                                                <  2:780798/150‑1 (MQ=255)
                                                        tggCGGTTTCGCAGCCAGGTTCAGGGTCTGGTACAATTCGCCAATCGCCGCAACCAGTTAGTTACGGACAATTTAGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACATCATGACCAACCGCTTTCTCATATACGCGATAGATaaa                                                                                               >  2:819071/2‑150 (MQ=255)
                                                           cGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTc                                                                                            <  2:2812213/150‑1 (MQ=255)
                                                            ggTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCa                                                                                           >  1:1092227/1‑150 (MQ=255)
                                                              ttGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACg                                                                                         <  2:113528/150‑1 (MQ=255)
                                                               tGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGc                                                                                        >  2:2711113/1‑150 (MQ=255)
                                                                gcgcAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCa                                                                                       <  2:2228685/150‑1 (MQ=255)
                                                                gcgcAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCa                                                                                       <  1:1515368/150‑1 (MQ=255)
                                                                 cgcAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCAc                                                                                      >  2:4485049/1‑150 (MQ=255)
                                                                   cAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTa                                                                                    <  1:3031655/150‑1 (MQ=255)
                                                                   cAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTa                                                                                    <  1:3399410/150‑1 (MQ=255)
                                                                      ccAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCg                                                                                 <  1:1891185/150‑1 (MQ=255)
                                                                       cAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCgt                                                                                >  1:1145674/1‑149 (MQ=255)
                                                                         ggTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCa                                                                              <  2:2098788/150‑1 (MQ=255)
                                                                         ggTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCa                                                                              >  2:3960621/1‑150 (MQ=255)
                                                                              aGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCt                                                                         <  1:2200588/150‑1 (MQ=255)
                                                                              aGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCt                                                                         <  1:1713478/150‑1 (MQ=255)
                                                                              aGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCt                                                                         <  2:1738428/150‑1 (MQ=255)
                                                                                  tCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGt                                                                     >  2:2382301/1‑150 (MQ=255)
                                                                                   cTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTAATTAACTTAATTACCAACCGCTTTCTCTATTACGCGATTGATCAACTCACGCACTACCGGCAGAGCTACGTc                                                                    >  1:465967/1‑150 (MQ=255)
                                                                                    tGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTcc                                                                   <  1:4066537/150‑1 (MQ=255)
                                                                                      gTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGc                                                                 >  1:4337255/1‑150 (MQ=255)
                                                                                        tctcTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCt                                                               <  2:1043056/150‑1 (MQ=255)
                                                                                         ctctTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTc                                                              >  1:2918048/1‑150 (MQ=255)
                                                                                                cccATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCGCTTATTAACTTCATGACCAACCGCTTTCACTTTAACGCGATAGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCagcagc                                                       >  1:1997349/1‑150 (MQ=255)
                                                                                                    tCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCa                                                   <  1:2808153/150‑1 (MQ=255)
                                                                                                     cgccgcAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCAt                                                  >  1:4266974/1‑150 (MQ=255)
                                                                                                     cgccgcAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCAt                                                  <  1:2581001/150‑1 (MQ=255)
                                                                                                          cAACCGGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCa                                             <  1:1252016/150‑1 (MQ=255)
                                                                                                          cAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCa                                             <  2:3251186/150‑1 (MQ=255)
                                                                                                             ccAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACtt                                          <  1:276511/150‑1 (MQ=255)
                                                                                                             ccAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACtt                                          <  1:702582/150‑1 (MQ=255)
                                                                                                              cAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTc                                         <  1:4194376/150‑1 (MQ=255)
                                                                                                                gTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTcgc                                       <  1:701743/150‑1 (MQ=255)
                                                                                                                 ttCGTTACGGACTATTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTcgcg                                      <  2:3870484/150‑1 (MQ=255)
                                                                                                                    gTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAg                                   >  1:3894943/1‑150 (MQ=255)
                                                                                                                    gTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAg                                   <  1:3847986/150‑1 (MQ=255)
                                                                                                                     ttACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGc                                  >  2:2489737/1‑150 (MQ=255)
                                                                                                                      tACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCg                                 <  1:1009166/150‑1 (MQ=255)
                                                                                                                      tACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCg                                 <  2:2563487/150‑1 (MQ=255)
                                                                                                                      tACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCg                                 >  1:854213/1‑150 (MQ=255)
                                                                                                                            cTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCtt                           >  1:912181/1‑150 (MQ=255)
                                                                                                                              aTTTTGACGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCtttt                         >  2:2440977/1‑150 (MQ=255)
                                                                                                                                     cGAACTCCTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGt                  <  1:714897/150‑1 (MQ=255)
                                                                                                                                      gAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGGATCTTTTACGTTGTc                 >  1:1787394/1‑150 (MQ=255)
                                                                                                                                         cTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTc              <  2:2420750/150‑1 (MQ=255)
                                                                                                                                         cTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTc              <  2:4120533/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCa             >  1:4384695/1‑150 (MQ=255)
                                                                                                                                          tCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGGATCTTTTACGTTGTCTTCa             >  2:2744449/1‑150 (MQ=255)
                                                                                                                                            cTGCCCCGGAGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGt           >  2:4631303/1‑150 (MQ=255)
                                                                                                                                             tGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTg          >  2:1795443/1‑150 (MQ=255)
                                                                                                                                              gCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGa         <  2:1890457/150‑1 (MQ=255)
                                                                                                                                              gCCCCGGAGTCAGGCTCTTATTAACTTCCTGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGa         <  2:1480397/150‑1 (MQ=255)
                                                                                                                                                   ggCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGt    >  2:1663696/1‑150 (MQ=255)
                                                                                                                                                   ggCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGt    >  2:1663582/1‑150 (MQ=255)
                                                                                                                                                     cGTCAGGCTCTTATTAACTTCCTGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTcc  <  1:4557482/150‑1 (MQ=255)
                                                                                                                                                     cGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTcc  >  2:3775174/1‑150 (MQ=255)
                                                                                                                                                     cGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTcc  <  2:3527906/150‑1 (MQ=255)
                                                                                                                                                     cGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTcc  >  2:2562587/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACCAACGCTGGTTGTTTTACCGGCACCTTGCAGGCCCGCCATCAGTACGACCGCAGGCGGTTGCGCAGCCAGGTTCAGGGTCTGGTTCTCTTCGCCCATCGCCGCAACCAGTTCGTTACGGACTATTTTGACGAACTCCTGCCCCGGCGTCAGGCTCTTATTAACTTCATGACCAACCGCTTTCTCTTTTACGCGATTGATAAACTCACGCACTACCGGCAGAGCTACGTCCGCCTCCAGCAGCGCCATGCGCACTTCGCGCAGCGTATCTTTTACGTTGTCTTCAGTGAGGCGTCC  >  CP009273/2740799‑2741097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A