Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,741,288 C→A 38.6% intergenic (‑134/‑33) ffh ← / → ypjD Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)/cytochrome c assembly protein family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,741,2880CA38.6% 352.7 / 287.1 264intergenic (‑134/‑33)ffh/ypjDSignal Recognition Particle (SRP) component with 4.5S RNA (ffs)/cytochrome c assembly protein family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (73/89);  new base A (54/48);  total (127/137)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 275 total aligned reads displayed.

TAAATTATCAAACATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGG  >  CP009273/2741140‑2741435
                                                                                                                                                    |                                                                                                                                                   
  aaTTATCAAACATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTctct                                                                                                                                                  <  2:1936068/150‑1 (MQ=255)
   aTTATCAAACATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTAtctt                                                                                                                                                 <  1:1148578/150‑1 (MQ=255)
     tATCAAACATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCATTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCtctttc                                                                                                                                               <  1:1718092/150‑1 (MQ=255)
         aaaCATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTAt                                                                                                                                           <  1:3905962/150‑1 (MQ=255)
         aaaCATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTAt                                                                                                                                           >  2:415025/1‑150 (MQ=255)
           aCATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTg                                                                                                                                         <  2:4005534/150‑1 (MQ=255)
           aCATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTGTCTTATTg                                                                                                                                         >  2:3934762/1‑150 (MQ=255)
              ttGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTc                                                                                                                                      <  2:940504/150‑1 (MQ=255)
                gTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCaa                                                                                                                                    >  1:1261681/1‑150 (MQ=255)
                 tctctcGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCaaa                                                                                                                                   <  1:1674570/150‑1 (MQ=255)
                  ctctcGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACAACATCAGTATATTGCCGCAGTATAACAAGAAGCCGTCTATGATGTTATTCAACTGTAGTCGCAGCTTACACCTGACGCTATACTGCTTCTCTGTCTTATTGCTCAAAc                                                                                                                                  >  1:2808235/1‑150 (MQ=255)
                   tctcGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACt                                                                                                                                 <  2:606334/150‑1 (MQ=255)
                    ctcGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTg                                                                                                                                >  2:3760218/1‑150 (MQ=255)
                     tcGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGt                                                                                                                               >  1:2410765/1‑150 (MQ=255)
                      cGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTc                                                                                                                              <  2:1392833/150‑1 (MQ=255)
                      cGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTc                                                                                                                              <  1:1845428/150‑1 (MQ=255)
                       gCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCg                                                                                                                             <  1:1710948/150‑1 (MQ=255)
                         cTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGAc                                                                                                                           >  1:3301689/1‑150 (MQ=255)
                         cTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGAc                                                                                                                           >  1:1088362/1‑150 (MQ=255)
                               tGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACt                                                                                                                     >  2:1174193/1‑150 (MQ=255)
                                ggAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTa                                                                                                                    >  1:2623820/1‑150 (MQ=255)
                                 gAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTAt                                                                                                                   <  2:251269/150‑1 (MQ=255)
                                   aaCGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGc                                                                                                                 >  2:460924/1‑150 (MQ=255)
                                     cGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGT‑GACATCACTATGCCCg                                                                                                              >  2:1696366/1‑150 (MQ=255)
                                      ggTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCg                                                                                                              <  2:1439704/150‑1 (MQ=255)
                                       gTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGt                                                                                                             <  2:3122439/150‑1 (MQ=255)
                                         tGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGttt                                                                                                           >  2:536384/1‑150 (MQ=255)
                                           gCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGttttt                                                                                                         <  2:1482458/150‑1 (MQ=255)
                                           gCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGttttt                                                                                                         <  2:1591279/150‑1 (MQ=255)
                                            ccGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTtg                                                                                                        <  2:1251283/150‑1 (MQ=255)
                                             agCAATCGCGACACATCAACAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGACGACATCACTATGCCCTTTTTtgc                                                                                                       >  2:3379777/2‑150 (MQ=255)
                                              gCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTtgct                                                                                                      >  2:1755200/1‑150 (MQ=255)
                                              gCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTtgct                                                                                                      >  1:1383097/1‑150 (MQ=255)
                                              gCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTtgct                                                                                                      >  2:4499944/1‑150 (MQ=255)
                                                  tCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTtgctctgc                                                                                                  <  1:2184227/150‑1 (MQ=255)
                                                        cacaTCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCgcgc                                                                                            >  2:2163440/1‑150 (MQ=255)
                                                          catcatCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCtt                                                                                          <  1:1899162/150‑1 (MQ=255)
                                                          catcatCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCtt                                                                                          >  2:277704/1‑150 (MQ=255)
                                                           atcatcAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTg                                                                                         <  2:1489978/150‑1 (MQ=255)
                                                               tcaGTATTTTGCCGCTGTATAACATGAAGGCGTATTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGTCGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCAATATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGc                                                                                     <  1:873346/150‑1 (MQ=255)
                                                                    aTTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACt                                                                                >  2:2689012/1‑150 (MQ=255)
                                                                     ttttGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTc                                                                               >  2:4387428/1‑150 (MQ=255)
                                                                      tttGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTcc                                                                              <  1:2270547/150‑1 (MQ=255)
                                                                        tGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGt                                                                            >  1:4303634/1‑150 (MQ=255)
                                                                        tGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGt                                                                            >  1:4047948/1‑150 (MQ=255)
                                                                         gCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTc                                                                           <  2:889820/150‑1 (MQ=255)
                                                                         gCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTc                                                                           <  2:889956/150‑1 (MQ=255)
                                                                          ccGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCa                                                                          <  2:3248198/150‑1 (MQ=255)
                                                                          ccGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCa                                                                          >  2:4069466/1‑150 (MQ=255)
                                                                            gCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGt                                                                        >  2:3393026/1‑150 (MQ=255)
                                                                              aGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCt                                                                      >  2:58942/1‑150 (MQ=255)
                                                                              aGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCt                                                                      <  1:1365835/150‑1 (MQ=255)
                                                                                  taACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTgcg                                                                  >  2:2386374/1‑150 (MQ=255)
                                                                                    aCATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCt                                                                <  2:4219601/150‑1 (MQ=255)
                                                                                     cATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTg                                                               <  2:1869584/150‑1 (MQ=255)
                                                                                     cATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTg                                                               <  1:582936/150‑1 (MQ=255)
                                                                                      aTGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGa                                                              <  1:3003085/150‑1 (MQ=255)
                                                                                      aTGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGa                                                              <  2:3801289/150‑1 (MQ=255)
                                                                                        gAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGAtt                                                            <  2:781225/150‑1 (MQ=255)
                                                                                        gAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGAtt                                                            >  1:2019134/1‑150 (MQ=255)
                                                                                         aaGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTg                                                           <  2:1315409/150‑1 (MQ=255)
                                                                                         aaGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTg                                                           >  2:835311/1‑150 (MQ=255)
                                                                                           ggCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGtt                                                         >  2:2429198/1‑150 (MQ=255)
                                                                                              gTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTccc                                                      <  2:575076/150‑1 (MQ=255)
                                                                                              gTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTccc                                                      <  1:3445888/150‑1 (MQ=255)
                                                                                               tCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCg                                                     <  2:2830353/150‑1 (MQ=255)
                                                                                               tCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCg                                                     <  2:3703990/150‑1 (MQ=255)
                                                                                                cTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCgg                                                    <  1:3774451/150‑1 (MQ=255)
                                                                                                 tttGTTGTTAAGAAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTAACTTTCTTATTGCTCAAACTGACGACATCACTATGCCCGTATTTGCTCTGCACGCGCTTGTCGCCTACTACGTCAGTCTTGCGCTGATTGTTCCCGGt                                                   >  2:2585546/1‑150 (MQ=255)
                                                                                                  ttgttgTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTc                                                  >  1:4465915/1‑150 (MQ=255)
                                                                                                   tgttgtTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTct                                                 <  2:2348179/150‑1 (MQ=255)
                                                                                                   tgttgtTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTct                                                 <  1:412985/150‑1 (MQ=255)
                                                                                                    gttgttATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTctg                                                <  2:3468353/150‑1 (MQ=255)
                                                                                                    gttgttATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTctg                                                >  2:926375/1‑150 (MQ=255)
                                                                                                     ttgttATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTctgc                                               <  1:2226408/150‑1 (MQ=255)
                                                                                                     ttgttATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTctgc                                               <  1:3134889/150‑1 (MQ=255)
                                                                                                             cAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCaaaaa                                       >  2:4579505/1‑150 (MQ=255)
                                                                                                             cAACGGTTCGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCACAAACTGTCGACATCACAATGCCCGTATTTGCTCTCCTCGCGCTAGTCGCCTACTCCGACAGTCTTGCGCTGAATGTTCCCGGTCTGCTGCaaaaa                                       >  2:3179797/1‑150 (MQ=255)
                                                                                                                  gTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAAcgg                                  >  1:371262/1‑150 (MQ=255)
                                                                                                                    tGGAGCAGCGTTCTCCTGACGCGATACTGCTTCTCTTTCTTATTGCTCATACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAATAcggcg                                <  2:4618210/150‑1 (MQ=255)
                                                                                                                    tGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAAcggcg                                <  1:4560556/150‑1 (MQ=255)
                                                                                                                    tGGAGCAGCGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAAcggcg                                >  1:2466786/1‑150 (MQ=255)
                                                                                                                     ggAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAAcggcgg                               <  1:1799528/150‑1 (MQ=255)
                                                                                                                     ggAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAAcggcgg                               >  1:2676656/1‑150 (MQ=255)
                                                                                                                          agcGTTCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTggc                          >  2:214431/1‑150 (MQ=255)
                                                                                                                            cGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTggcgg                        >  2:1082022/1‑150 (MQ=255)
                                                                                                                             gTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTggcggc                       <  2:3043055/150‑1 (MQ=255)
                                                                                                                             gTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTggcggc                       <  1:553012/150‑1 (MQ=255)
                                                                                                                             gTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTggcggc                       >  1:2715695/1‑150 (MQ=255)
                                                                                                                              ttCACCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGgcggcg                      >  1:143205/1‑150 (MQ=255)
                                                                                                                                cACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCa                    >  1:3622698/1‑150 (MQ=255)
                                                                                                                                 aCCTGACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCAt                   <  2:2858531/150‑1 (MQ=255)
                                                                                                                                     gACGCTATACTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCt               >  2:4376270/1‑150 (MQ=255)
                                                                                                                                        gCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCtatt            <  2:3619089/150‑1 (MQ=255)
                                                                                                                                         cTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTTCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCTGTCTGCTGCAAAAAAACGGCTGCTGGCGGCGCATGGCtatta           >  2:2098047/1‑150 (MQ=255)
                                                                                                                                          tataCTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCCGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCtattat          <  1:3204741/150‑1 (MQ=255)
                                                                                                                                           ataCTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTattatt         >  1:2430670/1‑150 (MQ=255)
                                                                                                                                            taCTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTAttt        >  2:2371047/1‑150 (MQ=255)
                                                                                                                                             aCTGCTTATCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTc       >  1:819209/1‑150 (MQ=255)
                                                                                                                                                 cTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTATGCTCGCGCTTGTCGCATACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCg   <  1:114202/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAAATTATCAAACATTGTCTCTCGCCTGGGGTGGAAACGGTTGGCCGCAATCGCGACACATCATCAGTATTTTGCCGCAGTATAACATGAAGGCGTCTTTGTTGTTATGCAACGGTTGGAGCAGCGTTCACCTGACGCTATACTGCTTCTCTTTCTTATTGCTCAAACTGTCGACATCACTATGCCCGTTTTTGCTCTGCTCGCGCTTGTCGCCTACTCCGTCAGTCTTGCGCTGATTGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGG  >  CP009273/2741140‑2741435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A