Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,753,424 G→T 57.8% G361* (GGA→TGA)  yfjI → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,753,4240GT57.8% 26.2 / 300.0 185G361* (GGA→TGA) yfjICP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (30/48);  new base T (37/70);  total (67/118)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 185 total aligned reads displayed.

TTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAGATT  >  CP009273/2753298‑2753571
                                                                                                                              |                                                                                                                                                   
ttCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCa                                                                                                                              <  1:2698108/150‑1 (MQ=31)
ttCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCa                                                                                                                              <  1:2698017/150‑1 (MQ=31)
  cTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGt                                                                                                                            <  2:53726/150‑1 (MQ=32)
  cTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGt                                                                                                                            <  2:1690359/150‑1 (MQ=32)
   tACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTa                                                                                                                           >  1:527903/1‑150 (MQ=18)
          aGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAagcag                                                                                                                    <  1:2114515/150‑1 (MQ=255)
          aGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAagcag                                                                                                                    >  2:28992/1‑150 (MQ=255)
            gTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAgcagca                                                                                                                  >  1:1828409/1‑150 (MQ=255)
              cGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGt                                                                                                                >  2:1713568/1‑150 (MQ=255)
              cGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGt                                                                                                                <  2:1957987/150‑1 (MQ=255)
              cGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGt                                                                                                                <  2:1958002/150‑1 (MQ=255)
               gAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTg                                                                                                               <  1:2279527/150‑1 (MQ=255)
               gAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTg                                                                                                               >  2:1841353/1‑150 (MQ=255)
                aGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTgg                                                                                                              <  1:4255098/150‑1 (MQ=255)
                 gTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGga                                                                                                             >  1:560284/1‑150 (MQ=255)
                 gTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGga                                                                                                             >  1:2336486/1‑150 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAATGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  2:3557062/150‑1 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  2:3557030/150‑1 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  1:1140972/150‑1 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  1:3131749/150‑1 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  2:3703963/150‑1 (MQ=255)
                        aTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTg                                                                                                      <  1:1140950/150‑1 (MQ=255)
                                 aTTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGtat                                                                                             <  2:4471221/150‑1 (MQ=255)
                                      ccTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTga                                                                                        <  1:1568714/150‑1 (MQ=255)
                                      ccTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTga                                                                                        >  2:2440767/1‑150 (MQ=255)
                                       cttcttTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTgaa                                                                                       <  1:2264245/150‑1 (MQ=255)
                                        ttcttTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTgaag                                                                                      <  2:3892990/150‑1 (MQ=255)
                                        ttcttTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTgaag                                                                                      <  2:2491067/150‑1 (MQ=255)
                                          cttTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTgaagaa                                                                                    >  1:932031/1‑150 (MQ=255)
                                            ttATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATa                                                                                  <  2:2855062/150‑1 (MQ=255)
                                            ttATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATa                                                                                  <  2:410680/150‑1 (MQ=255)
                                             tATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGATATATTGAAGAATAc                                                                                 >  1:920188/1‑150 (MQ=255)
                                               tGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAt                                                                               >  1:4061010/1‑150 (MQ=255)
                                               tGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAt                                                                               >  1:2429587/1‑150 (MQ=255)
                                                gATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATc                                                                              <  2:258101/150‑1 (MQ=255)
                                                 aTTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGATGCTTGCTGCCTTACTTCATTACTTCAGAGGTGATGGAGGCGATATATCTGATACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAAcc                                                                             >  1:1382907/1‑150 (MQ=255)
                                                 aTTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATcc                                                                             >  1:3259677/1‑150 (MQ=255)
                                                  ttttAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCg                                                                            <  2:2523212/150‑1 (MQ=255)
                                                    ttAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCt                                                                          >  2:4403395/1‑150 (MQ=255)
                                                    ttAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCt                                                                          >  2:4403422/1‑150 (MQ=255)
                                                    ttAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCt                                                                          >  2:2073520/1‑150 (MQ=255)
                                                     tAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCtt                                                                         >  2:3947004/1‑150 (MQ=255)
                                                     tAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCtt                                                                         <  1:1813423/150‑1 (MQ=255)
                                                     tAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCtt                                                                         >  2:1618062/1‑150 (MQ=255)
                                                     tAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTAATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCtt                                                                         <  2:1560602/150‑1 (MQ=255)
                                                       aGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGt                                                                       >  2:1870682/1‑150 (MQ=255)
                                                        ggATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGtt                                                                      <  1:3544233/150‑1 (MQ=255)
                                                        ggATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGtt                                                                      <  2:1343826/150‑1 (MQ=255)
                                                         gATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTtc                                                                     >  1:2319858/1‑150 (MQ=255)
                                                           ttATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTtctc                                                                   >  2:3317352/1‑150 (MQ=255)
                                                           ttATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTtctc                                                                   <  1:4544911/150‑1 (MQ=255)
                                                             aTGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTa                                                                 >  2:2542318/1‑150 (MQ=255)
                                                             aTGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTa                                                                 >  1:2544349/1‑150 (MQ=255)
                                                              tGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTaa                                                                <  2:4104879/150‑1 (MQ=255)
                                                                    tAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAaaga                                                          <  2:4606499/150‑1 (MQ=255)
                                                                    tAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAaaga                                                          >  2:4130221/1‑150 (MQ=255)
                                                                      aaaTGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAaagaag                                                        <  1:3804767/150‑1 (MQ=255)
                                                                      aaaTGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAaagaag                                                        >  1:1212632/1‑150 (MQ=255)
                                                                       aaTGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAagaaga                                                       <  1:4236201/150‑1 (MQ=255)
                                                                         tGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGt                                                     <  2:1739782/150‑1 (MQ=255)
                                                                         tGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGt                                                     <  2:2418702/150‑1 (MQ=255)
                                                                          ggCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGtt                                                    >  1:393154/1‑150 (MQ=255)
                                                                           gCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGttt                                                   <  1:150217/150‑1 (MQ=255)
                                                                             ggAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTc                                                 >  1:3236005/1‑150 (MQ=255)
                                                                               agaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCtt                                               <  1:4154340/150‑1 (MQ=255)
                                                                                gaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCttt                                              <  1:2079253/150‑1 (MQ=255)
                                                                                gaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCttt                                              <  1:519692/150‑1 (MQ=255)
                                                                                gaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCttt                                              <  2:16419/150‑1 (MQ=255)
                                                                                gaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCttt                                              >  2:3914785/1‑150 (MQ=255)
                                                                                gaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCttt                                              >  2:2136563/1‑150 (MQ=255)
                                                                                  aCATGGCGAGGCTTGCTGCCTTACTTCTTTACTTCAGCGTTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCATCAGTGGTGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAg                                            <  1:4618043/150‑1 (MQ=37)
                                                                                  aCATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAg                                            >  1:4169322/1‑150 (MQ=255)
                                                                                    aTGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAt                                          <  1:477611/150‑1 (MQ=255)
                                                                                    aTGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAt                                          >  1:739590/1‑150 (MQ=255)
                                                                                    aTGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAt                                          <  2:1069030/150‑1 (MQ=255)
                                                                                      ggCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGt                                        <  2:3014289/150‑1 (MQ=255)
                                                                                      ggCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGt                                        >  2:2283779/1‑150 (MQ=255)
                                                                                         gAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTc                                     >  1:3074911/1‑150 (MQ=255)
                                                                                            gCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGa                                  <  1:639046/150‑1 (MQ=255)
                                                                                             cTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGaa                                 >  2:2277211/1‑150 (MQ=255)
                                                                                             cTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGaa                                 <  1:4528731/150‑1 (MQ=255)
                                                                                              ttGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAg                                <  1:4115199/150‑1 (MQ=255)
                                                                                              ttGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAg                                >  1:3578553/1‑150 (MQ=255)
                                                                                               tgctgcCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGc                               <  2:2192961/150‑1 (MQ=255)
                                                                                               tgctgcCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGc                               <  2:3856497/150‑1 (MQ=255)
                                                                                                gctgcCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCa                              >  2:942176/1‑150 (MQ=255)
                                                                                                 ctgcCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAg                             <  2:2346363/150‑1 (MQ=255)
                                                                                                       tacttcattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGc                       >  2:1443109/1‑150 (MQ=255)
                                                                                                           tcattacttcaGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTa                   <  2:4284157/150‑1 (MQ=255)
                                                                                                             attacttcaGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTAtt                 >  1:258662/1‑150 (MQ=255)
                                                                                                             attacttcaGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTAtt                 <  2:3339898/150‑1 (MQ=255)
                                                                                                               tacttcaGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTAttgt               <  1:3092826/150‑1 (MQ=255)
                                                                                                                acttcaGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTAttgtt              >  1:4185497/1‑150 (MQ=255)
                                                                                                                 cttcaGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTAttgttg             >  2:4221292/1‑150 (MQ=255)
                                                                                                                  ttcaGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTgg            <  1:60731/150‑1 (MQ=255)
                                                                                                                  ttcaGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTgg            >  2:1312560/1‑150 (MQ=255)
                                                                                                                     aGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATa         <  2:3823753/150‑1 (MQ=255)
                                                                                                                         gTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAg     >  1:1965867/1‑150 (MQ=255)
                                                                                                                           gATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAGAt   <  2:560284/150‑1 (MQ=255)
                                                                                                                            aTGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAGAtt  >  2:216583/1‑150 (MQ=255)
                                                                                                                              |                                                                                                                                                   
TTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAGATT  >  CP009273/2753298‑2753571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAG  >  CP009273/2753276‑2753568
                                                                                                                                                    |                                                                                                                                                
AGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGG                                                                                                                                                 >  SRR19973072.1651409/1‑150 (MQ=0)
 GCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGA                                                                                                                                                <  SRR19973072.233615/150‑1 (MQ=0)
   AGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGG                                                                                                                                              >  SRR19973072.198848/1‑150 (MQ=0)
    GAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGC                                                                                                                                             >  SRR19973072.3803656/1‑150 (MQ=0)
    GAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGC                                                                                                                                             >  SRR19973072.1932945/1‑150 (MQ=0)
    GAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGC                                                                                                                                             >  SRR19973072.1932725/1‑150 (MQ=0)
    GAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGC                                                                                                                                             >  SRR19973072.3831061/1‑150 (MQ=0)
     AAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCG                                                                                                                                            >  SRR19973072.4156025/1‑150 (MQ=0)
     AAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCG                                                                                                                                            <  SRR19973072.52968/150‑1 (MQ=0)
     AAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCG                                                                                                                                            >  SRR19973072.2169470/1‑150 (MQ=0)
      AAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGA                                                                                                                                           <  SRR19973072.1123538/150‑1 (MQ=0)
      AAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGA                                                                                                                                           >  SRR19973072.1508001/1‑150 (MQ=0)
       AGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGAT                                                                                                                                          >  SRR19973072.1637322/1‑150 (MQ=0)
        GACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATA                                                                                                                                         >  SRR19973072.2306203/1‑150 (MQ=0)
          CATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATA                                                                                                                                       <  SRR19973072.3918853/150‑1 (MQ=0)
          CATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATA                                                                                                                                       >  SRR19973072.660588/1‑150 (MQ=0)
              GGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTG                                                                                                                                   >  SRR19973072.3858585/1‑150 (MQ=0)
                   GAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACC                                                                                                                              <  SRR19973072.2034611/150‑1 (MQ=0)
                   GAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACC                                                                                                                              <  SRR19973072.3694300/150‑1 (MQ=0)
                    AATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGGGATGGAGGCGATATATCTGTTACCG                                                                                                                             >  SRR19973072.1480091/1‑150 (MQ=0)
                      TTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCA                                                                                                                           <  SRR19973072.2703850/150‑1 (MQ=1)
                      TTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCA                                                                                                                           <  SRR19973072.2703759/150‑1 (MQ=1)
                      TTATACAACCAGGTCGAGTCAGAAATGAGAATGTTTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGATGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCA                                                                                                                           <  SRR19973072.2703847/150‑1 (MQ=1)
                         TACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTA                                                                                                                        >  SRR19973072.529161/1‑150 (MQ=8)
                                AGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAG                                                                                                                 <  SRR19973072.2119113/150‑1 (MQ=25)
                                AGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAG                                                                                                                 <  SRR19973072.2119126/150‑1 (MQ=25)
                                  GTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCA                                                                                                               >  SRR19973072.1832391/1‑150 (MQ=29)
                                     GAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTG                                                                                                            <  SRR19973072.2284418/150‑1 (MQ=36)
                                     GAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTG                                                                                                            <  SRR19973072.2284470/150‑1 (MQ=36)
                                      AGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGG                                                                                                           <  SRR19973072.4263944/150‑1 (MQ=38)
                                       GTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGA                                                                                                          >  SRR19973072.561596/1‑150 (MQ=40)
                                       GTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGA                                                                                                          >  SRR19973072.2341498/1‑150 (MQ=40)
                                         CAGAAATGAGAATGATTGGCCTTC‑TTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGAT                                                                                                       <  SRR19973072.4192814/150‑1 (MQ=44)
                                              ATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTG                                                                                                   <  SRR19973072.1143337/150‑1 (MQ=55)
                                              ATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTG                                                                                                   <  SRR19973072.3138356/150‑1 (MQ=55)
                                              ATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTG                                                                                                   <  SRR19973072.3500150/150‑1 (MQ=55)
                                              ATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTG                                                                                                   <  SRR19973072.1143359/150‑1 (MQ=55)
                                               TGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGG                                                                                                  <  SRR19973072.1604522/150‑1 (MQ=58)
                                                    ATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGG                                                                                             >  SRR19973072.2276602/1‑150 (MQ=60)
                                                      GATTGTCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGATAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTA                                                                                           <  SRR19973072.2892790/150‑1 (MQ=60)
                                                       ATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTAT                                                                                          <  SRR19973072.313616/150‑1 (MQ=60)
                                                            CCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGA                                                                                     <  SRR19973072.1572063/150‑1 (MQ=60)
                                                             CTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAA                                                                                    <  SRR19973072.2269117/150‑1 (MQ=60)
                                                             CTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAA                                                                                    <  SRR19973072.2269099/150‑1 (MQ=60)
                                                               TCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGA                                                                                  >  SRR19973072.637016/1‑150 (MQ=60)
                                                                CTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAA                                                                                 >  SRR19973072.934064/1‑150 (MQ=60)
                                                                   TATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGATATATTGAAGAATAC                                                                              >  SRR19973072.922187/1‑150 (MQ=60)
                                                                     TGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAT                                                                            >  SRR19973072.4069527/1‑150 (MQ=60)
                                                                     TGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAT                                                                            >  SRR19973072.2434855/1‑150 (MQ=60)
                                                                       ATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGATGCTTGCTGCCTTACTTCATTACTTCAGAGGTGATGGAGGCGATATATCTGATACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACAACC                                                                          >  SRR19973072.1385920/1‑150 (MQ=60)
                                                                       ATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCC                                                                          >  SRR19973072.3266596/1‑150 (MQ=60)
                                                                       ATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCC                                                                          <  SRR19973072.1438638/150‑1 (MQ=60)
                                                                          TTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCT                                                                       <  SRR19973072.2654645/150‑1 (MQ=60)
                                                                           TAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTT                                                                      <  SRR19973072.1817374/150‑1 (MQ=60)
                                                                              GGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTT                                                                   <  SRR19973072.3551685/150‑1 (MQ=60)
                                                                              GGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTT                                                                   <  SRR19973072.3551985/150‑1 (MQ=60)
                                                                               GATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTC                                                                  >  SRR19973072.671108/1‑150 (MQ=60)
                                                                               GATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTC                                                                  >  SRR19973072.2324822/1‑150 (MQ=60)
                                                                                 TTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTC                                                                <  SRR19973072.4554490/150‑1 (MQ=60)
                                                                                 TTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTC                                                                <  SRR19973072.4554496/150‑1 (MQ=60)
                                                                                   ATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTA                                                              >  SRR19973072.2549875/1‑150 (MQ=60)
                                                                                       TTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAA                                                          >  SRR19973072.2558021/1‑150 (MQ=60)
                                                                                            AAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAG                                                     >  SRR19973072.1215224/1‑150 (MQ=60)
                                                                                            AAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAG                                                     <  SRR19973072.3812734/150‑1 (MQ=60)
                                                                                             AATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGA                                                    <  SRR19973072.4245076/150‑1 (MQ=60)
                                                                                             AATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGA                                                    <  SRR19973072.4245022/150‑1 (MQ=60)
                                                                                               TGGCGGAGAACTTGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATGCGCTTGTTCTCTAAAAAAGAAGAGT                                                  <  SRR19973072.1523110/150‑1 (MQ=60)
                                                                                                GGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTT                                                 >  SRR19973072.394010/1‑150 (MQ=60)
                                                                                                 GCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTT                                                <  SRR19973072.150601/150‑1 (MQ=60)
                                                                                                   GGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTC                                              >  SRR19973072.3242869/1‑150 (MQ=60)
                                                                                                    GAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCT                                             <  SRR19973072.4096387/150‑1 (MQ=60)
                                                                                                    GAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCT                                             <  SRR19973072.561100/150‑1 (MQ=60)
                                                                                                    GAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCT                                             <  SRR19973072.3182719/150‑1 (MQ=60)
                                                                                                     AGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTT                                            <  SRR19973072.4163049/150‑1 (MQ=60)
                                                                                                      GAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTT                                           <  SRR19973072.2083782/150‑1 (MQ=60)
                                                                                                      GAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTT                                           <  SRR19973072.520932/150‑1 (MQ=60)
                                                                                                      GAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTT                                           <  SRR19973072.4526186/150‑1 (MQ=60)
                                                                                                        ACATGGCGAGGCTTGCTGCCTTACTTCTTTACTTCAGCGTTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCATCAGTGGTGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAG                                         <  SRR19973072.4627723/150‑1 (MQ=60)
                                                                                                        ACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAG                                         >  SRR19973072.4178053/1‑150 (MQ=60)
                                                                                                          ATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAT                                       <  SRR19973072.478630/150‑1 (MQ=60)
                                                                                                          ATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAT                                       <  SRR19973072.478732/150‑1 (MQ=60)
                                                                                                          ATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAT                                       >  SRR19973072.741175/1‑150 (MQ=60)
                                                                                                          ATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGAT                                       <  SRR19973072.478752/150‑1 (MQ=60)
                                                                                                           TGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATG                                      <  SRR19973072.3863636/150‑1 (MQ=60)
                                                                                                             GCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTT                                    <  SRR19973072.1207201/150‑1 (MQ=60)
                                                                                                               GAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTC                                  >  SRR19973072.3081403/1‑150 (MQ=60)
                                                                                                                  GCTTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGA                               <  SRR19973072.640500/150‑1 (MQ=60)
                                                                                                                   CTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAA                              <  SRR19973072.4538291/150‑1 (MQ=60)
                                                                                                                    TTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAG                             <  SRR19973072.4123867/150‑1 (MQ=60)
                                                                                                                    TTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAG                             <  SRR19973072.4123841/150‑1 (MQ=60)
                                                                                                                    TTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAG                             >  SRR19973072.3586087/1‑150 (MQ=60)
                                                                                                                    TTGCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAG                             <  SRR19973072.2344805/150‑1 (MQ=60)
                                                                                                                      GCTGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCA                           <  SRR19973072.2491611/150‑1 (MQ=60)
                                                                                                                      GCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCA                           <  SRR19973072.1312612/150‑1 (MQ=60)
                                                                                                                        TGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGA                         >  SRR19973072.4165026/1‑150 (MQ=60)
                                                                                                                        TGCCTTACTTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGA                         >  SRR19973072.3950218/1‑150 (MQ=60)
                                                                                                                                TTCATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTT                 <  SRR19973072.4602868/150‑1 (MQ=60)
                                                                                                                                   ATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAgactggccccctgaatctccagacaaccaatatcacttaaataagtga              >  SRR19973072.1511154/1‑102 (MQ=60)
                                                                                                                                   ATTACTTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATT              >  SRR19973072.259278/1‑150 (MQ=60)
                                                                                                                                   ATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATT              >  SRR19973072.893427/1‑150 (MQ=60)
                                                                                                                                     TACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGT            <  SRR19973072.3099347/150‑1 (MQ=60)
                                                                                                                                      ACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTT           >  SRR19973072.4194256/1‑150 (MQ=60)
                                                                                                                                        TTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGG         <  SRR19973072.60893/150‑1 (MQ=60)
                                                                                                                                        TTCAGCGGTGATTGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGG         <  SRR19973072.60894/150‑1 (MQ=60)
                                                                                                                                        TTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGG         <  SRR19973072.1981874/150‑1 (MQ=60)
                                                                                                                                           AGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATA      >  SRR19973072.3246024/1‑150 (MQ=60)
                                                                                                                                               GTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAG  >  SRR19973072.1970065/1‑150 (MQ=60)
                                                                                                                                               GTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAG  >  SRR19973072.1970073/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                
AGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATTGAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCAGATGAGCTTTATTGTTGGATAAAAG  >  CP009273/2753276‑2753568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑