Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,035,271 G→T 16.4% V200V (GTG→GTT)  ygfZ → iron‑sulfur cluster repair protein, plumbagin resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,035,2710GT16.4% 814.6 / 89.2 252V200V (GTG→GTT) ygfZiron‑sulfur cluster repair protein, plumbagin resistance
Reads supporting (aligned to +/- strand):  ref base G (105/105);  new base T (20/22);  total (125/127)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTGGTT  >  CP009273/3035122‑3035420
                                                                                                                                                     |                                                                                                                                                     
ttGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtg                                                                                                                                                       >  2:4072114/1‑150 (MQ=255)
   cTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgatt                                                                                                                                                    <  2:738993/150‑1 (MQ=255)
      tGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGtgattgat                                                                                                                                                 <  2:1347223/150‑1 (MQ=255)
       ggTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATg                                                                                                                                                <  1:3572472/150‑1 (MQ=255)
          ttGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATgccg                                                                                                                                             <  1:2329377/150‑1 (MQ=255)
           tGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATgccgc                                                                                                                                            >  1:4255341/1‑150 (MQ=255)
             aaCACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCa                                                                                                                                          >  1:236875/1‑150 (MQ=255)
              acacCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCaa                                                                                                                                         <  1:1283138/150‑1 (MQ=255)
              acacCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCaa                                                                                                                                         <  2:2589326/150‑1 (MQ=255)
                 cccGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAg                                                                                                                                      >  2:1846280/1‑150 (MQ=255)
                   cGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCg                                                                                                                                    <  1:2724743/150‑1 (MQ=255)
                    ggCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCgg                                                                                                                                   <  2:103912/150‑1 (MQ=255)
                     gCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCggg                                                                                                                                  <  1:868690/150‑1 (MQ=255)
                       aGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTTGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCa                                                                                                                                >  2:2075152/1‑150 (MQ=255)
                          aCGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGtt                                                                                                                             >  2:848220/1‑150 (MQ=255)
                            gTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTa                                                                                                                           <  2:4030723/150‑1 (MQ=255)
                             tttCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTAt                                                                                                                          <  1:3956317/150‑1 (MQ=255)
                              ttCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATc                                                                                                                         <  2:2627816/150‑1 (MQ=255)
                              ttCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATc                                                                                                                         >  1:1823137/1‑150 (MQ=255)
                               tCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATcc                                                                                                                        >  2:343764/1‑150 (MQ=255)
                               tCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATcc                                                                                                                        <  2:1761437/150‑1 (MQ=255)
                                 cTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCca                                                                                                                      <  1:4027177/150‑1 (MQ=255)
                                 cTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCca                                                                                                                      <  1:3310531/150‑1 (MQ=255)
                                  tGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCcac                                                                                                                     >  1:1246826/1‑150 (MQ=255)
                                   gATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCcaca                                                                                                                    <  1:3199612/150‑1 (MQ=255)
                                     tCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAgg                                                                                                                  <  2:4617940/150‑1 (MQ=255)
                                      cGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGc                                                                                                                 >  2:2097250/1‑150 (MQ=255)
                                        tAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGa                                                                                                               >  1:3716706/1‑150 (MQ=255)
                                           ccGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCa                                                                                                            <  2:3482955/150‑1 (MQ=255)
                                              aTGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtc                                                                                                         >  1:1351019/1‑150 (MQ=255)
                                              aTGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtc                                                                                                         >  1:3014759/1‑150 (MQ=255)
                                              aTGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtc                                                                                                         <  2:2917307/150‑1 (MQ=255)
                                               tGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtct                                                                                                        >  1:142557/1‑150 (MQ=255)
                                               tGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtct                                                                                                        <  1:1106625/150‑1 (MQ=255)
                                                gAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAAtctc                                                                                                       >  1:3422175/1‑150 (MQ=255)
                                                 aaGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTcc                                                                                                      >  2:757909/1‑150 (MQ=255)
                                                  aGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCa                                                                                                     >  1:4383069/1‑150 (MQ=255)
                                                  aGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCa                                                                                                     <  1:534216/150‑1 (MQ=255)
                                                  aGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAAAAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCa                                                                                                     >  1:1539449/1‑150 (MQ=255)
                                                  aGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGAGTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCa                                                                                                     >  2:1528934/1‑150 (MQ=255)
                                                    cTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAgg                                                                                                   <  1:640366/150‑1 (MQ=255)
                                                    cTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAgg                                                                                                   >  2:340078/1‑150 (MQ=255)
                                                    cTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAgg                                                                                                   <  2:641880/150‑1 (MQ=255)
                                                    cTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAgg                                                                                                   <  2:4501343/150‑1 (MQ=255)
                                                     tACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGgc                                                                                                  >  1:388494/1‑150 (MQ=255)
                                                       cTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGgcgc                                                                                                >  1:2617524/1‑150 (MQ=255)
                                                        tGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCt                                                                                               <  2:4136721/150‑1 (MQ=255)
                                                         gCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTg                                                                                              <  2:431700/150‑1 (MQ=255)
                                                         gCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTg                                                                                              <  1:4570815/150‑1 (MQ=255)
                                                         gCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTg                                                                                              <  1:1019101/150‑1 (MQ=255)
                                                            aaTATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGc                                                                                           >  2:814679/1‑150 (MQ=255)
                                                             atatGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCg                                                                                          >  2:3810851/1‑150 (MQ=255)
                                                             atatGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCg                                                                                          >  2:3239698/1‑150 (MQ=255)
                                                                 gCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTAt                                                                                      <  1:1295559/150‑1 (MQ=255)
                                                                    gACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAg                                                                                   <  2:19070/150‑1 (MQ=255)
                                                                      ccGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCt                                                                                 >  2:528329/1‑150 (MQ=255)
                                                                        gATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGCAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTGTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCttt                                                                               <  2:1117562/150‑1 (MQ=255)
                                                                        gATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCttt                                                                               <  2:3085545/150‑1 (MQ=255)
                                                                        gATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCttt                                                                               <  2:248238/150‑1 (MQ=255)
                                                                            aaCTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGa                                                                           >  1:3873213/1‑150 (MQ=255)
                                                                             aCTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGaa                                                                          >  1:745140/1‑150 (MQ=255)
                                                                              cTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGaaa                                                                         <  2:2461822/150‑1 (MQ=255)
                                                                               tGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAg                                                                        <  2:3302644/150‑1 (MQ=255)
                                                                                  gcgGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCt                                                                     <  2:2355141/150‑1 (MQ=255)
                                                                                  gcgGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCt                                                                     <  2:3613530/150‑1 (MQ=255)
                                                                                  gcgGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCt                                                                     >  1:199751/1‑150 (MQ=255)
                                                                                    ggTGAAGCGGAACTGAACAATAGCCAACAGTGGCTAGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGt                                                                   <  2:824507/150‑1 (MQ=255)
                                                                                      tGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTta                                                                 >  1:3084585/1‑150 (MQ=255)
                                                                                       gAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTtat                                                                <  1:2268468/150‑1 (MQ=255)
                                                                                            ggAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCgg                                                           >  1:2911264/1‑150 (MQ=255)
                                                                                              aaCTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGAc                                                         >  1:3640516/1‑150 (MQ=255)
                                                                                                cTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACaa                                                       <  2:1630339/150‑1 (MQ=255)
                                                                                                cTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACaa                                                       <  2:4062109/150‑1 (MQ=255)
                                                                                                    aCAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGACAGGCTGTTATACCGGACAAgaga                                                   >  2:2435492/1‑150 (MQ=255)
                                                                                                     cAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAt                                                  >  2:3716498/1‑150 (MQ=255)
                                                                                                       aTAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAtgg                                                <  1:1833627/150‑1 (MQ=255)
                                                                                                        tAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAtggt                                               <  2:1370659/150‑1 (MQ=255)
                                                                                                        tAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGAtggt                                               <  2:3515291/150‑1 (MQ=255)
                                                                                                           ccAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGgc                                            <  2:2864156/150‑1 (MQ=255)
                                                                                                            cAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGgcg                                           <  1:1687732/150‑1 (MQ=255)
                                                                                                               cAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGa                                        >  1:1079704/1‑150 (MQ=255)
                                                                                                                aGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAg                                       <  1:1469198/150‑1 (MQ=255)
                                                                                                                  tggctggcATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCa                                     >  2:1941225/1‑150 (MQ=255)
                                                                                                                      tggcATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAAt                                 <  1:4135665/150‑1 (MQ=255)
                                                                                                                        gcATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTc                               <  2:4239904/150‑1 (MQ=255)
                                                                                                                          aTTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCg                             >  2:1264696/1‑150 (MQ=255)
                                                                                                                              aaCATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACCGGCGACCAATCTCCTGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTTTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCgtggt                         <  2:236946/150‑1 (MQ=255)
                                                                                                                              aaCATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCgtggt                         >  1:2417534/1‑150 (MQ=255)
                                                                                                                                 aTTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGcc                      >  2:3260524/1‑150 (MQ=255)
                                                                                                                                   tGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCaa                    >  2:2190737/1‑150 (MQ=255)
                                                                                                                                           gTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTATAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGAGGTGCCAATAAACGTg            >  1:1706891/1‑150 (MQ=255)
                                                                                                                                             ttCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTgcg          >  2:2339610/1‑150 (MQ=255)
                                                                                                                                               cccGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGct        >  2:558629/1‑150 (MQ=255)
                                                                                                                                                ccGGTTATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGctc       >  2:2821990/1‑150 (MQ=255)
                                                                                                                                                ccGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGctc       >  1:2552897/1‑150 (MQ=255)
                                                                                                                                                  ggTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTg     <  2:4305796/150‑1 (MQ=255)
                                                                                                                                                  ggTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTg     <  2:1823138/150‑1 (MQ=255)
                                                                                                                                                    tgattgatGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTGGt   >  2:3756770/1‑150 (MQ=255)
                                                                                                                                                    tgattgatGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTGGt   <  1:4467978/150‑1 (MQ=255)
                                                                                                                                                     gattgatGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTGGtt  <  1:2595355/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGCTGACCGATAAACTGCGCGGTGAAGCGGAACTGAACAATAGCCAACAGTGGCTGGCATTAAACATTGAAGCGGGTTTCCCGGTGATTGATGCCGCCAACAGCGGGCAGTTTATCCCACAGGCGACCAATCTCCAGGCGCTGGGCGGTATCAGCTTTAAGAAAGGCTGTTATACCGGACAAGAGATGGTGGCGCGAGCAAAATTCCGTGGTGCCAATAAACGTGCGCTCTGGTT  >  CP009273/3035122‑3035420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A