Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,036,052 C→A 18.1% S152S (TCG→TCT)  yqfA ← hemolysin III family HylIII inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,036,0520CA18.1% 745.8 / 105.7 248S152S (TCG→TCT) yqfAhemolysin III family HylIII inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (105/98);  new base A (26/19);  total (131/117)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 249 total aligned reads displayed.

GCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCCCACCAGCAA  >  CP009273/3035904‑3036201
                                                                                                                                                    |                                                                                                                                                     
gCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCg                                                                                                                                                      <  2:3013391/150‑1 (MQ=255)
gCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCg                                                                                                                                                      <  2:694249/150‑1 (MQ=255)
 cACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGa                                                                                                                                                     <  2:23570/150‑1 (MQ=255)
  aCGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAc                                                                                                                                                    >  1:3314287/1‑150 (MQ=255)
    gAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcag                                                                                                                                                  <  1:2704601/150‑1 (MQ=255)
     aaGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagc                                                                                                                                                 >  1:259634/1‑150 (MQ=255)
       gCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcca                                                                                                                                               <  1:3981938/150‑1 (MQ=255)
          gTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACagccagcc                                                                                                                                            >  2:3356472/1‑150 (MQ=255)
          gTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACagccagcc                                                                                                                                            >  1:2221912/1‑150 (MQ=255)
             ccAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCAt                                                                                                                                         <  1:3869415/150‑1 (MQ=255)
              cAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATc                                                                                                                                        <  1:1085296/150‑1 (MQ=255)
              cAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATc                                                                                                                                        <  2:1437541/150‑1 (MQ=255)
               aGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCg                                                                                                                                       <  2:4562577/150‑1 (MQ=255)
                       tGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAg                                                                                                                               >  1:2189401/1‑150 (MQ=255)
                         gTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGt                                                                                                                             >  1:1127818/1‑150 (MQ=255)
                          ttGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTc                                                                                                                            >  1:494928/1‑150 (MQ=255)
                          ttGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTc                                                                                                                            <  2:1527369/150‑1 (MQ=255)
                             tATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCAcc                                                                                                                         >  2:4011706/1‑150 (MQ=255)
                               tGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCag                                                                                                                       >  2:393150/1‑150 (MQ=255)
                               tGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCag                                                                                                                       >  2:4279743/1‑150 (MQ=255)
                                ggAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCaga                                                                                                                      >  1:1683990/1‑150 (MQ=255)
                                 gAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCagag                                                                                                                     <  1:3255678/150‑1 (MQ=255)
                                  aaTGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAgaga                                                                                                                    >  2:1077811/1‑150 (MQ=255)
                                      cGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaaa                                                                                                                >  1:2272801/1‑150 (MQ=255)
                                       gTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaaaa                                                                                                               <  2:386564/150‑1 (MQ=255)
                                               aCGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAAt                                                                                                       <  1:2818963/150‑1 (MQ=255)
                                               aCGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAAt                                                                                                       <  1:3752800/150‑1 (MQ=255)
                                                cGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATc                                                                                                      <  1:1462166/150‑1 (MQ=255)
                                                 gTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCg                                                                                                     <  1:3134618/150‑1 (MQ=255)
                                                 gTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCg                                                                                                     <  2:2597952/150‑1 (MQ=255)
                                                  tAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCgg                                                                                                    >  2:2250062/1‑150 (MQ=255)
                                                   aGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGt                                                                                                   >  1:1763380/1‑150 (MQ=255)
                                                   aGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGt                                                                                                   >  2:807571/1‑150 (MQ=255)
                                                   aGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGt                                                                                                   <  1:1963385/150‑1 (MQ=255)
                                                        aTCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGcgcg                                                                                              <  2:1499770/150‑1 (MQ=255)
                                                          cACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGAt                                                                                            <  1:2311798/150‑1 (MQ=255)
                                                          cACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGAt                                                                                            >  1:403946/1‑150 (MQ=255)
                                                           aCCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATg                                                                                           >  2:1186709/1‑150 (MQ=255)
                                                            ccccGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATgg                                                                                          <  2:1770433/150‑1 (MQ=255)
                                                            ccccGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATgg                                                                                          <  2:4620603/150‑1 (MQ=255)
                                                              ccGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTc                                                                                        >  1:2454521/1‑150 (MQ=255)
                                                               cGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCa                                                                                       <  2:500439/150‑1 (MQ=255)
                                                                  gCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGtt                                                                                    >  2:1745241/1‑150 (MQ=255)
                                                                     aaTAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTaa                                                                                 >  2:1325594/1‑150 (MQ=255)
                                                                          aCCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGa                                                                            >  1:569273/1‑150 (MQ=255)
                                                                                ccgccTACCGCGAGTAATTTAACGCTGACCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTATACAGATTAccc                                                                      <  2:588272/150‑1 (MQ=255)
                                                                                 cgccTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCa                                                                     <  2:246995/150‑1 (MQ=255)
                                                                                 cgccTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCa                                                                     <  2:3041246/150‑1 (MQ=255)
                                                                                 cgccTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCa                                                                     <  1:1607164/150‑1 (MQ=255)
                                                                                    cTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCa                                                                  >  2:2911122/1‑150 (MQ=255)
                                                                                    cTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCa                                                                  >  2:566467/1‑150 (MQ=255)
                                                                                    cTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAATCAGAATACCCAGCa                                                                  >  2:2447802/1‑150 (MQ=255)
                                                                                       ccGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATg                                                               <  2:3387875/150‑1 (MQ=255)
                                                                                       ccGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATg                                                               <  2:2003857/150‑1 (MQ=255)
                                                                                        cGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGc                                                              >  1:1320688/1‑150 (MQ=255)
                                                                                          ccAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCa                                                            >  1:68626/1‑150 (MQ=255)
                                                                                            aGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAgg                                                          >  2:2030902/1‑150 (MQ=255)
                                                                                               aaGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTc                                                       >  1:915779/1‑150 (MQ=255)
                                                                                               aaGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTc                                                       >  2:3449828/1‑150 (MQ=255)
                                                                                                 ggTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCa                                                     <  2:2289981/150‑1 (MQ=255)
                                                                                                 ggTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCa                                                     <  2:2781816/150‑1 (MQ=255)
                                                                                                 ggTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCa                                                     >  1:2564542/1‑150 (MQ=255)
                                                                                                  gTAACGCTGCCCGCCGCGAGCTTAACTTCCATTACAGAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGATATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAATCAGAATTCCCAGCAATGCCAGGCTCCAg                                                    <  2:3966757/150‑1 (MQ=255)
                                                                                                       gCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAAc                                               <  2:3287911/150‑1 (MQ=255)
                                                                                                       gCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAAc                                               <  2:3539169/150‑1 (MQ=255)
                                                                                                       gCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAAc                                               >  2:3986099/1‑150 (MQ=255)
                                                                                                       acTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAAc                                               >  2:3621176/2‑150 (MQ=255)
                                                                                                         tGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGTCAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAActa                                             <  1:907089/150‑3 (MQ=255)
                                                                                                         tGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACaa                                             >  2:3229098/1‑150 (MQ=255)
                                                                                                             cgccgcGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAatcat                                         >  2:2679735/1‑150 (MQ=255)
                                                                                                             cgccgcGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAatcat                                         >  1:322540/1‑150 (MQ=255)
                                                                                                               ccgcGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAAtcatca                                       <  2:2222131/150‑1 (MQ=255)
                                                                                                                  cgAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAAcc                                    >  1:111104/1‑150 (MQ=255)
                                                                                                                   gAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAAccc                                   <  1:3054080/150‑1 (MQ=255)
                                                                                                                   gAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAAccc                                   <  2:2845263/150‑1 (MQ=255)
                                                                                                                    aGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCcg                                  >  2:665476/1‑150 (MQ=255)
                                                                                                                     gCTTAACTGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCcgc                                 >  2:166291/1‑150 (MQ=255)
                                                                                                                      cttatatGCCATTTCATAAATTACCACCAGAGACAGCCAGCCCATCTCCATATAGGTCACCAGAGATAAAATTTTGATTCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCcgcg                                <  1:2062613/144‑1 (MQ=255)
                                                                                                                       ttAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCcgcgc                               >  2:1623380/1‑150 (MQ=255)
                                                                                                                       ttAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCcgcgc                               <  1:4605786/150‑1 (MQ=255)
                                                                                                                         aaCTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAAGACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCgcgcgc                             <  2:3768661/150‑1 (MQ=255)
                                                                                                                           cTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTa                           >  2:2065216/1‑150 (MQ=255)
                                                                                                                           cTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTa                           >  2:3489244/1‑150 (MQ=255)
                                                                                                                           cTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTa                           >  2:1176057/1‑150 (MQ=255)
                                                                                                                              ccATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACg                        <  2:3194969/150‑1 (MQ=255)
                                                                                                                               cATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACgg                       >  1:2456173/1‑150 (MQ=255)
                                                                                                                               cATTTCATAAATTACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACgg                       >  1:3196882/1‑150 (MQ=255)
                                                                                                                                 tttCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGgag                     <  1:1492946/150‑1 (MQ=255)
                                                                                                                                  ttCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGgaga                    <  1:3579733/150‑1 (MQ=255)
                                                                                                                                   tCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGaa                   >  1:4183956/1‑150 (MQ=255)
                                                                                                                                   tCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGaa                   <  2:4447659/150‑1 (MQ=255)
                                                                                                                                    cATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAAt                  <  1:2779848/150‑1 (MQ=255)
                                                                                                                                          ttACCACCAGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGcc            >  1:2474917/1‑150 (MQ=255)
                                                                                                                                            accaccAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGcccc          >  2:2207229/1‑150 (MQ=255)
                                                                                                                                              caccaGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCTCGCTAACGGAGAATCCAGCCccac        >  2:2726791/1‑150 (MQ=255)
                                                                                                                                               accaGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCccacc       >  1:3472944/1‑150 (MQ=255)
                                                                                                                                               accaGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCccacc       >  2:1102196/1‑150 (MQ=255)
                                                                                                                                                ccaGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCccacca      >  2:1411523/1‑150 (MQ=255)
                                                                                                                                                ccaGAGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCccacca      >  2:947457/1‑150 (MQ=255)
                                                                                                                                                  aGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCCCACCAGc    <  1:3405292/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAAACAGAATACCCAGCAATGCCAGGCTCCAGATAACAATCATCAACCCGCGCGCTAACGGAGAATCCAGCCCCACCAGCAA  >  CP009273/3035904‑3036201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A