Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,046,245 C→A 31.0% A211A (GCG→GCT)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,046,2450CA31.0% 645.4 / 257.3 315A211A (GCG→GCT) ubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (107/110);  new base A (59/39);  total (166/149)
Fisher's exact test for biased strand distribution p-value = 8.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

CAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATC  >  CP009273/3046097‑3046394
                                                                                                                                                    |                                                                                                                                                     
   cGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCa                                                                                                                                                   >  2:2804184/1‑150 (MQ=255)
     tGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGc                                                                                                                                                 >  1:4087652/1‑150 (MQ=255)
        gTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCggg                                                                                                                                              <  1:3162835/150‑1 (MQ=255)
         tAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGc                                                                                                                                             >  2:1824846/1‑150 (MQ=255)
          aaTTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGcc                                                                                                                                            <  2:3734378/150‑1 (MQ=255)
               tCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGtt                                                                                                                                       >  1:1114765/1‑150 (MQ=255)
               tCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGtt                                                                                                                                       <  1:4466209/150‑1 (MQ=255)
                 ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGc                                                                                                                                     >  1:2506648/1‑150 (MQ=255)
                 ccAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGc                                                                                                                                     >  1:3411583/1‑150 (MQ=255)
                    aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTa                                                                                                                                  <  1:3518708/150‑1 (MQ=255)
                         ccagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCg                                                                                                                             <  2:1635033/150‑1 (MQ=255)
                          cagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGt                                                                                                                            >  1:2894881/1‑150 (MQ=255)
                           agccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGtt                                                                                                                           <  1:3112926/150‑1 (MQ=255)
                              caAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCaa                                                                                                                        <  2:3039393/150‑1 (MQ=255)
                              caAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCaa                                                                                                                        >  2:638106/1‑150 (MQ=255)
                                aaGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCaaaa                                                                                                                      >  2:1282517/1‑150 (MQ=255)
                                 aGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAg                                                                                                                     <  1:3901760/150‑1 (MQ=255)
                                 aGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAg                                                                                                                     <  2:4142229/150‑1 (MQ=255)
                                   gCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcg                                                                                                                   >  1:1667035/1‑150 (MQ=255)
                                          ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCtt                                                                                                            <  2:391523/150‑1 (MQ=255)
                                           gAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTc                                                                                                           >  2:347726/1‑150 (MQ=255)
                                           gAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTc                                                                                                           <  2:62494/150‑1 (MQ=255)
                                               tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgc                                                                                                       <  1:3660810/150‑1 (MQ=255)
                                                cACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATgcg                                                                                                      <  2:3995347/150‑1 (MQ=255)
                                                  cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGTGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                    >  2:2469973/1‑150 (MQ=255)
                                                  cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                    <  1:4309628/150‑1 (MQ=255)
                                                  cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                    <  1:3085946/150‑1 (MQ=255)
                                                  cGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                                    <  1:1980377/150‑1 (MQ=255)
                                                      aaaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACgg                                                                                                >  1:2598064/1‑150 (MQ=255)
                                                       aaaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGa                                                                                               >  1:4213479/1‑150 (MQ=255)
                                                        aaCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGaa                                                                                              >  1:493873/1‑150 (MQ=255)
                                                         aCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAg                                                                                             >  2:1581256/1‑150 (MQ=255)
                                                         aCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAg                                                                                             <  1:2063152/150‑1 (MQ=255)
                                                          cTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGt                                                                                            <  1:3016401/150‑1 (MQ=255)
                                                          cTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGt                                                                                            <  2:582925/150‑1 (MQ=255)
                                                           ttCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTa                                                                                           >  2:1504054/1‑150 (MQ=255)
                                                           ttCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTa                                                                                           >  2:2686227/1‑150 (MQ=255)
                                                             ctcGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGc                                                                                         <  1:4378582/150‑1 (MQ=255)
                                                                  cACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGt                                                                                    >  1:2910291/1‑150 (MQ=255)
                                                                   aCTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGtt                                                                                   >  1:4073096/1‑150 (MQ=255)
                                                                     tCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                                 >  1:2723540/1‑150 (MQ=255)
                                                                         cGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAAt                                                                             >  2:2406587/1‑150 (MQ=255)
                                                                          gACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATc                                                                            >  2:1267452/1‑150 (MQ=255)
                                                                          gACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATc                                                                            >  1:693755/1‑150 (MQ=255)
                                                                          gACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGAGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATc                                                                            <  1:3076960/150‑1 (MQ=255)
                                                                            caacaCCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCAc                                                                          <  2:2000776/150‑1 (MQ=255)
                                                                            caacaCCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCAc                                                                          >  2:681311/1‑150 (MQ=255)
                                                                             aacaCCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACg                                                                         >  1:3372561/1‑150 (MQ=255)
                                                                              acacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACgg                                                                        <  1:2351833/150‑1 (MQ=255)
                                                                                acCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGcc                                                                      <  2:3079932/150‑1 (MQ=255)
                                                                                  ctctTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAg                                                                    >  2:2922736/1‑150 (MQ=255)
                                                                                       cgcgCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgttgtt                                                               >  2:4021215/1‑150 (MQ=255)
                                                                                       cgcgCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgttgtt                                                               <  2:4525807/150‑1 (MQ=255)
                                                                                       cgcgCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgttgtt                                                               <  1:1315830/150‑1 (MQ=255)
                                                                                        gcgcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTc                                                              >  1:564088/1‑150 (MQ=255)
                                                                                        gcgcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTc                                                              <  1:3563216/150‑1 (MQ=255)
                                                                                           ccGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTa                                                           >  2:3791970/1‑150 (MQ=255)
                                                                                           ccGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTa                                                           <  1:1603050/150‑1 (MQ=255)
                                                                                           ccGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTa                                                           >  2:2243747/1‑150 (MQ=255)
                                                                                            cGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTaa                                                          >  1:2125572/1‑150 (MQ=255)
                                                                                            cGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTaa                                                          >  1:2525275/1‑150 (MQ=255)
                                                                                              ttCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAgg                                                        <  1:346673/150‑1 (MQ=255)
                                                                                               tCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGc                                                       <  1:3289429/150‑1 (MQ=255)
                                                                                                ccAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCt                                                      >  2:1876226/1‑150 (MQ=255)
                                                                                                  aGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTcc                                                    <  1:2274660/150‑1 (MQ=255)
                                                                                                   gTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCct                                                   >  2:3413934/1‑150 (MQ=255)
                                                                                                   gTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCct                                                   <  2:3213202/150‑1 (MQ=255)
                                                                                                    tGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctg                                                  >  1:45975/1‑150 (MQ=255)
                                                                                                      gATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctgct                                                <  1:1923082/150‑1 (MQ=255)
                                                                                                       aTGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctgctg                                               >  2:1004762/1‑150 (MQ=255)
                                                                                                        tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                              <  2:930166/150‑1 (MQ=255)
                                                                                                        tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                              >  1:3137067/1‑150 (MQ=255)
                                                                                                          acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                            <  1:1412912/150‑1 (MQ=255)
                                                                                                          acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                            <  2:4462629/150‑1 (MQ=255)
                                                                                                          acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                            >  1:4405050/1‑150 (MQ=255)
                                                                                                          acacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCa                                            <  1:1785877/150‑1 (MQ=255)
                                                                                                           cacCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                           <  2:1411132/150‑1 (MQ=255)
                                                                                                            accagaccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGt                                          >  1:4218184/1‑150 (MQ=255)
                                                                                                                gaccagCGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAc                                      <  1:4577140/150‑1 (MQ=255)
                                                                                                                 accagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcg                                     <  2:3267212/150‑1 (MQ=255)
                                                                                                                   cagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgcc                                   <  1:2875950/150‑1 (MQ=255)
                                                                                                                   cagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgcc                                   <  2:560878/150‑1 (MQ=255)
                                                                                                                   cagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgcc                                   <  2:1336150/150‑1 (MQ=255)
                                                                                                                   cagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgcc                                   <  2:1721568/150‑1 (MQ=255)
                                                                                                                      cGAACAGCGTCCGTCAGACATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCa                                <  2:4270397/150‑1 (MQ=255)
                                                                                                                          cAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgcg                            >  2:893199/1‑150 (MQ=255)
                                                                                                                          cAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgcg                            <  1:3720286/150‑1 (MQ=255)
                                                                                                                          cAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGACGCAcgcg                            >  1:941884/1‑150 (MQ=255)
                                                                                                                                      gACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCt                <  1:760658/150‑1 (MQ=255)
                                                                                                                                        cATCGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGa              <  1:2846745/150‑1 (MQ=255)
                                                                                                                                         aTCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGaa             >  1:4619839/1‑150 (MQ=255)
                                                                                                                                           cGGCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATg           <  1:4009136/150‑1 (MQ=255)
                                                                                                                                            ggCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg          >  2:1200912/1‑150 (MQ=255)
                                                                                                                                            ggCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg          >  2:3226281/1‑150 (MQ=255)
                                                                                                                                            ggCAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg          >  2:4240398/1‑150 (MQ=255)
                                                                                                                                             gCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATggg         >  2:3185974/1‑150 (MQ=255)
                                                                                                                                              cAACATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCCTGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGt        <  1:38450/150‑1 (MQ=255)
                                                                                                                                               aaCATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTg       <  2:3209582/150‑1 (MQ=255)
                                                                                                                                               aaCATAGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTg       >  2:3687387/1‑150 (MQ=255)
                                                                                                                                                  aTCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCa    <  2:210886/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGGGTGCCATC  >  CP009273/3046097‑3046394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A