Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,091,476 C→A 41.7% T284K (ACG→AAG)  yggW → HemN family putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,091,4760CA41.7% 345.9 / inf 301T284K (ACG→AAG) yggWHemN family putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (97/78);  new base A (59/67);  total (156/145)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 310 total aligned reads displayed.

CAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCTTT  >  CP009273/3091328‑3091625
                                                                                                                                                    |                                                                                                                                                     
cAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcg                                                                                                                                                      <  1:4031542/150‑1 (MQ=255)
 aaTATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAagc                                                                                                                                                     <  2:3261586/150‑1 (MQ=255)
    atGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACAACGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGAACCACCAAAACGCGTc                                                                                                                                                  >  1:3950035/1‑150 (MQ=255)
        aaCTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATcc                                                                                                                                              >  2:4014025/1‑150 (MQ=255)
        aaCTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATcc                                                                                                                                              >  2:3728229/1‑150 (MQ=255)
        aaCTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATcc                                                                                                                                              >  2:1776739/1‑150 (MQ=255)
               gCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGt                                                                                                                                       >  1:1973943/1‑150 (MQ=255)
               gCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGt                                                                                                                                       <  2:1935142/150‑1 (MQ=255)
               gCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGt                                                                                                                                       <  2:457906/150‑1 (MQ=255)
                cTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGtt                                                                                                                                      >  1:3534306/1‑150 (MQ=255)
                 ttACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGttt                                                                                                                                     >  2:3502097/1‑150 (MQ=255)
                  tACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGtttt                                                                                                                                    <  2:4359356/150‑1 (MQ=255)
                     gCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATg                                                                                                                                 <  1:2340810/150‑1 (MQ=255)
                     gCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATg                                                                                                                                 >  2:1525425/1‑150 (MQ=255)
                      ccAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGc                                                                                                                                >  2:4178762/1‑150 (MQ=255)
                       cAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCa                                                                                                                               >  1:25563/1‑150 (MQ=255)
                       cAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCa                                                                                                                               >  2:2153949/1‑150 (MQ=255)
                        aaaCCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaa                                                                                                                              >  1:4302115/1‑150 (MQ=255)
                          aCCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCaagg                                                                                                                            <  2:953090/150‑1 (MQ=255)
                           cccGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                                                                           <  2:1569520/150‑1 (MQ=255)
                           cccGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                                                                           <  2:3897985/150‑1 (MQ=255)
                            caggTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaa                                                                                                                          >  1:2264665/3‑150 (MQ=255)
                              ggTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaagg                                                                                                                        >  1:3395359/1‑150 (MQ=255)
                               gTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGt                                                                                                                       >  1:4241616/1‑150 (MQ=255)
                                ttATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTa                                                                                                                      <  1:1618477/150‑1 (MQ=255)
                                ttATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTGTTATGCAAGGAAGGTa                                                                                                                      >  2:2261666/1‑150 (MQ=255)
                                  aTCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATc                                                                                                                    >  1:3344714/1‑150 (MQ=255)
                                   tCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCt                                                                                                                   <  2:529101/150‑1 (MQ=255)
                                    cAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTg                                                                                                                  <  2:3404877/150‑1 (MQ=255)
                                     aGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTgg                                                                                                                 <  2:2701052/150‑1 (MQ=255)
                                       tGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGGATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGaa                                                                                                               >  2:263759/1‑150 (MQ=255)
                                          cAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGc                                                                                                            >  1:3083402/1‑150 (MQ=255)
                                          cAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGc                                                                                                            >  2:2548216/1‑150 (MQ=255)
                                              acaATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGc                                                                                                        >  1:1689750/1‑150 (MQ=255)
                                              acaATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGc                                                                                                        <  2:1402666/150‑1 (MQ=255)
                                                aaTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGt                                                                                                      >  1:4532598/1‑150 (MQ=255)
                                                 aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTg                                                                                                     <  1:2087732/150‑1 (MQ=255)
                                                 aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTg                                                                                                     >  2:3306797/1‑150 (MQ=255)
                                                   ctcAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGAt                                                                                                   >  1:4073859/1‑150 (MQ=255)
                                                   ctcAACTACTGGCGCTTTGGTGACTACATCGGTAATGTCTGCGGCGCACACGGCAAAGTGACCATCACGGATGGGAGCATTCTGCGTACCACCAAAAAGCTTAATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGAt                                                                                                   >  2:4613139/1‑150 (MQ=255)
                                                    tccactactGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACCCGGCAAAGGGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTAGGCAAGGAAGGTATCTGGAAAGCCAGCGTGATg                                                                                                  <  2:1146705/147‑1 (MQ=255)
                                                       actactGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                                                                               >  2:1222453/1‑150 (MQ=255)
                                                            tGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAATGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGcc                                                                                          >  1:523115/1‑150 (MQ=255)
                                                            tGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGcc                                                                                          <  1:541664/150‑1 (MQ=255)
                                                             ggCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCca                                                                                         <  2:2040797/150‑1 (MQ=255)
                                                             ggCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCca                                                                                         <  2:1426028/150‑1 (MQ=255)
                                                               cgcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCcaca                                                                                       <  1:2720977/150‑1 (MQ=255)
                                                                gcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAg                                                                                      <  1:112882/150‑1 (MQ=255)
                                                                 cTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGa                                                                                     >  2:4296584/1‑150 (MQ=255)
                                                                   ttGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGAGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTGATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATa                                                                                   >  2:832360/1‑150 (MQ=255)
                                                                    tGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATaa                                                                                  <  2:2937990/150‑1 (MQ=255)
                                                                     ggTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAg                                                                                 >  2:967236/1‑150 (MQ=255)
                                                                      gTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGc                                                                                <  1:4241507/150‑1 (MQ=255)
                                                                       tGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGcc                                                                               <  2:766935/150‑1 (MQ=255)
                                                                         aCTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGt                                                                             >  1:882247/1‑150 (MQ=255)
                                                                          cTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTCTCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGtt                                                                            >  2:1471056/1‑150 (MQ=255)
                                                                           tACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGttt                                                                           >  2:2314100/1‑150 (MQ=255)
                                                                           tACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGatt                                                                           >  2:3021204/1‑150 (MQ=255)
                                                                            aCATCGGTATTGGCTGCGGCGCACACGGTAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTg                                                                          <  1:779820/150‑1 (MQ=255)
                                                                            aCATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTg                                                                          <  1:3747088/150‑1 (MQ=255)
                                                                            aCATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTg                                                                          >  2:4146889/1‑150 (MQ=255)
                                                                             cATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGa                                                                         <  2:313763/150‑1 (MQ=255)
                                                                             cATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGa                                                                         <  1:1885931/150‑1 (MQ=255)
                                                                              aTCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAg                                                                        >  2:4492247/1‑150 (MQ=255)
                                                                                  gTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCt                                                                    <  2:1540191/150‑1 (MQ=255)
                                                                                    aTTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCttt                                                                  <  1:3599797/150‑1 (MQ=255)
                                                                                     ttGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTa                                                                 <  2:3974271/150‑1 (MQ=255)
                                                                                        gCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGa                                                              <  1:1210832/150‑1 (MQ=255)
                                                                                          tGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAAt                                                            <  1:1013004/150‑1 (MQ=255)
                                                                                           gcggcgCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATc                                                           <  1:1501239/150‑1 (MQ=255)
                                                                                               cgcACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCtt                                                       <  2:1015906/150‑1 (MQ=255)
                                                                                                gcACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTc                                                      <  2:1674826/150‑1 (MQ=255)
                                                                                                  acacGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCg                                                    >  1:3858784/1‑150 (MQ=255)
                                                                                                    acGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTc                                                  >  2:3141699/1‑150 (MQ=255)
                                                                                                     cGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTct                                                 <  1:2969984/150‑1 (MQ=255)
                                                                                                      ggCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctg                                                >  1:2928338/1‑150 (MQ=255)
                                                                                                        cAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctgct                                              <  2:3776418/150‑1 (MQ=255)
                                                                                                          aaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTgg                                            >  2:3546617/1‑150 (MQ=255)
                                                                                                             tGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAgg                                         >  2:691419/1‑150 (MQ=255)
                                                                                                              gACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgc                                        <  2:2469862/150‑1 (MQ=255)
                                                                                                               acattCCCGGATGGGCGCATTCTGCGTACCACCTCAACGCGTCTGCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGATGCCACAGATAAGCCGTTTTAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgcc                                       <  1:3441383/147‑1 (MQ=255)
                                                                                                               aCCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgcc                                       <  1:3647740/150‑1 (MQ=255)
                                                                                                                ccTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccg                                      >  1:1861639/1‑150 (MQ=255)
                                                                                                                ccTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccg                                      <  2:2734719/150‑1 (MQ=255)
                                                                                                                  ttCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcg                                    >  2:1026499/1‑150 (MQ=255)
                                                                                                                    cccGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcgcc                                  <  1:3154239/150‑1 (MQ=255)
                                                                                                                    cccGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcgcc                                  <  2:3077718/150‑1 (MQ=255)
                                                                                                                      cGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcgccgc                                >  2:875584/1‑150 (MQ=255)
                                                                                                                         aTGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCcgcgcg                             >  1:2428309/1‑150 (MQ=255)
                                                                                                                         aTGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCcgcgcg                             >  2:2819752/1‑150 (MQ=255)
                                                                                                                           tgtcgcATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTg                           >  2:1846725/4‑150 (MQ=255)
                                                                                                                            ggCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTgg                          >  1:3716980/1‑150 (MQ=255)
                                                                                                                               gcATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGt                       <  1:2778901/150‑1 (MQ=255)
                                                                                                                                cATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGtt                      >  1:846298/1‑150 (MQ=255)
                                                                                                                                    cTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTAtt                  <  1:4608341/150‑1 (MQ=255)
                                                                                                                                      gCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGc                <  1:433748/150‑1 (MQ=255)
                                                                                                                                       cGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCg               <  1:455739/150‑1 (MQ=255)
                                                                                                                                         tACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGta             >  2:2437414/1‑150 (MQ=255)
                                                                                                                                            caccaAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATAc          >  1:1960386/1‑150 (MQ=255)
                                                                                                                                                    cgcgTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCttt  <  2:3789883/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCTTT  >  CP009273/3091328‑3091625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A