Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,091,476 C→A 6.2% T284K (ACG→AAG)  yggW → HemN family putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,091,4760CA 6.2% 1005.5 / 27.2 262T284K (ACG→AAG) yggWHemN family putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (121/124);  new base A (8/9);  total (129/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

GCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCTTT  >  CP009273/3091327‑3091625
                                                                                                                                                     |                                                                                                                                                     
gCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAc                                                                                                                                                       <  2:2867953/150‑1 (MQ=255)
 cAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcg                                                                                                                                                      <  1:3766021/150‑1 (MQ=255)
  aaTATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcgc                                                                                                                                                     >  2:3766874/1‑150 (MQ=255)
  aaTATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcgc                                                                                                                                                     >  1:2122462/1‑150 (MQ=255)
   atatGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTCTTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcgcg                                                                                                                                                    >  2:3792494/1‑150 (MQ=255)
   atatGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAcgcg                                                                                                                                                    >  2:2714026/1‑150 (MQ=255)
   atatGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAagcg                                                                                                                                                    <  1:3575572/150‑1 (MQ=255)
    tatGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGt                                                                                                                                                   >  2:606963/1‑150 (MQ=255)
     atGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTc                                                                                                                                                  <  2:1095008/150‑1 (MQ=255)
          aCTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCcg                                                                                                                                             >  1:158851/1‑150 (MQ=255)
            ttCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCcgcg                                                                                                                                           <  1:581239/150‑1 (MQ=255)
            ttCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACAACGGTATTGGCAGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCAGTCTGCCTACCACCAAAACGCGGCCGCcgcg                                                                                                                                           >  1:1949909/1‑150 (MQ=255)
              ccGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTg                                                                                                                                         <  1:1075041/150‑1 (MQ=255)
               cGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTgg                                                                                                                                        <  2:2175409/150‑1 (MQ=255)
                  ttACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGttt                                                                                                                                     >  1:2216489/1‑150 (MQ=255)
                  ttACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGttt                                                                                                                                     >  1:3872045/1‑150 (MQ=255)
                  ttACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGGGGttt                                                                                                                                     >  1:1522113/1‑150 (MQ=255)
                   tACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGtttt                                                                                                                                    <  1:3947871/150‑1 (MQ=255)
                   tACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGtttt                                                                                                                                    <  1:2888902/150‑1 (MQ=255)
                      gCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATg                                                                                                                                 >  1:2715163/1‑150 (MQ=255)
                           aCCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCaagg                                                                                                                            >  1:2799628/1‑150 (MQ=255)
                            cccGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                                                                           >  2:1987542/1‑150 (MQ=255)
                             ccGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaa                                                                                                                          >  1:3084929/1‑150 (MQ=255)
                              cGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaag                                                                                                                         <  2:3575861/150‑1 (MQ=255)
                               ggTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaagg                                                                                                                        <  2:2071739/150‑1 (MQ=255)
                                   aTCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATc                                                                                                                    >  2:4114695/1‑150 (MQ=255)
                                    tCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCt                                                                                                                   >  2:4014502/1‑150 (MQ=255)
                                         gCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGaaa                                                                                                              >  1:764677/1‑150 (MQ=255)
                                          ccAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAg                                                                                                             <  2:1396355/150‑1 (MQ=255)
                                            aGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGcc                                                                                                           <  1:4117783/150‑1 (MQ=255)
                                             gCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCa                                                                                                          <  1:2152066/150‑1 (MQ=255)
                                             gCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCa                                                                                                          <  1:2487258/150‑1 (MQ=255)
                                              cacaATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAg                                                                                                         <  1:3993107/150‑1 (MQ=255)
                                              cacaATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAg                                                                                                         >  1:4031910/1‑150 (MQ=255)
                                               acaATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGc                                                                                                        >  2:3301963/1‑150 (MQ=255)
                                                caATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCg                                                                                                       >  2:4545229/1‑150 (MQ=255)
                                                  aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTg                                                                                                     >  2:2259213/1‑150 (MQ=255)
                                                  aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTg                                                                                                     <  1:3918931/150‑1 (MQ=255)
                                                  aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTg                                                                                                     <  2:4475590/150‑1 (MQ=255)
                                                   tctcAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGa                                                                                                    >  1:4208371/1‑150 (MQ=255)
                                                   tctcAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGa                                                                                                    >  1:2540948/1‑150 (MQ=255)
                                                    ctcAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGAt                                                                                                   >  2:428703/1‑150 (MQ=255)
                                                     tcAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATg                                                                                                  >  2:3911721/1‑150 (MQ=255)
                                                      cAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGt                                                                                                 <  1:2602390/150‑1 (MQ=255)
                                                      cAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGt                                                                                                 <  1:2090495/150‑1 (MQ=255)
                                                       aaCTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTc                                                                                                <  2:2378267/150‑1 (MQ=255)
                                                        actactGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                                                                               <  2:2630663/150‑1 (MQ=255)
                                                        actactGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                                                                               <  2:4518221/150‑1 (MQ=255)
                                                         ctactGGCGCGTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGa                                                                                              >  1:4432219/1‑150 (MQ=255)
                                                           actGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAg                                                                                            >  2:4399096/1‑150 (MQ=255)
                                                             tGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGcc                                                                                          <  1:2272085/150‑1 (MQ=255)
                                                                   tttGGTGACTACATCGGTATTGGCTGCGGCGCACTCGGCAAAGTGAGCTTCCCGGATGGGCGGATTCTGCGTACCACCAAAACGCGTCTTCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGGGAGCGTGATGTCGAAGCCACAGAt                                                                                    <  2:2477613/150‑1 (MQ=255)
                                                                   tttGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGAt                                                                                    >  1:3468006/1‑150 (MQ=255)
                                                                   tttGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGAt                                                                                    <  1:3644106/150‑1 (MQ=255)
                                                                      ggTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAg                                                                                 <  1:4274325/150‑1 (MQ=255)
                                                                       gTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGc                                                                                <  2:1827471/150‑1 (MQ=255)
                                                                          aCTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCCGCGTGATGTCGAAGCCACAGATAAGCCGt                                                                             >  2:2576978/1‑150 (MQ=255)
                                                                          aCTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGt                                                                             <  1:1345523/150‑1 (MQ=255)
                                                                             aCATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTg                                                                          >  1:1032439/1‑150 (MQ=255)
                                                                              cATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGa                                                                         <  2:150684/150‑1 (MQ=255)
                                                                              cATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGa                                                                         <  1:1782281/150‑1 (MQ=255)
                                                                               aTCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTCTCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAg                                                                        >  2:3117006/1‑150 (MQ=255)
                                                                               aTCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAg                                                                        >  1:3428121/1‑150 (MQ=255)
                                                                                  ggTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTc                                                                     >  2:4462439/1‑150 (MQ=255)
                                                                                    tATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCtt                                                                   >  2:3460959/1‑150 (MQ=255)
                                                                                    tATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCtt                                                                   >  2:4306706/1‑150 (MQ=255)
                                                                                     aTTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCttt                                                                  >  1:4038060/1‑150 (MQ=255)
                                                                                           tGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAAt                                                            >  1:4566077/1‑150 (MQ=255)
                                                                                             cggcgCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCg                                                          <  2:2877432/150‑1 (MQ=255)
                                                                                               gcgcACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCt                                                        <  2:2998513/150‑1 (MQ=255)
                                                                                                 gcACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTc                                                      >  2:1662492/1‑150 (MQ=255)
                                                                                                 gcACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAAAGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTc                                                      >  2:1302502/1‑150 (MQ=255)
                                                                                                  cacaCGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTcc                                                     <  1:638811/150‑1 (MQ=255)
                                                                                                         cAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctgct                                              >  2:910859/1‑150 (MQ=255)
                                                                                                          aaaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctgctg                                             >  1:4019609/1‑150 (MQ=255)
                                                                                                          aaaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctgctg                                             <  2:3506434/150‑1 (MQ=255)
                                                                                                          aaaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTctgctg                                             >  2:4028862/1‑150 (MQ=255)
                                                                                                          aaaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTGTGAGTTCTTTATGAATCGCTTCCGTctgctg                                             >  1:4302767/1‑150 (MQ=255)
                                                                                                           aaGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTgg                                            >  1:2532143/1‑150 (MQ=255)
                                                                                                             gTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAg                                          >  1:3330429/1‑150 (MQ=255)
                                                                                                               gACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgc                                        >  2:723733/1‑150 (MQ=255)
                                                                                                                aCCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgcc                                       <  1:1105727/150‑1 (MQ=255)
                                                                                                                   ttCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcg                                    >  1:3596770/1‑150 (MQ=255)
                                                                                                                      ccGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGgccgcgccg                                 >  2:3411853/1‑150 (MQ=255)
                                                                                                                        ggATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGccgcgccgcg                               >  1:2054120/1‑150 (MQ=255)
                                                                                                                            gggCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTg                           <  1:773090/150‑1 (MQ=255)
                                                                                                                            gggCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTg                           >  2:1112117/1‑150 (MQ=255)
                                                                                                                                 cATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGtt                      >  2:3210639/1‑150 (MQ=255)
                                                                                                                                   ttCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTa                    >  1:3169197/1‑150 (MQ=255)
                                                                                                                                       gCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGc                <  2:3750413/150‑1 (MQ=255)
                                                                                                                                         gTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGGTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGt              >  2:1625292/1‑150 (MQ=255)
                                                                                                                                            ccaccaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGtata           <  1:1025591/150‑1 (MQ=255)
                                                                                                                                            ccaccaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGtata           <  1:861724/150‑1 (MQ=255)
                                                                                                                                             caccaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATAc          <  1:417870/150‑1 (MQ=255)
                                                                                                                                             caccaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATAc          >  2:865531/1‑150 (MQ=255)
                                                                                                                                              accaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATAcc         >  2:4592359/1‑150 (MQ=255)
                                                                                                                                              accaAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATAcc         >  2:2941626/1‑150 (MQ=255)
                                                                                                                                                 aaaaCGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCggg      >  1:1845449/1‑150 (MQ=255)
                                                                                                                                                   aaCGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCt    <  2:53184/150‑1 (MQ=255)
                                                                                                                                                    aCGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCtt   <  1:4434632/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCGCTTCCGTCTGCTGGAGGCCGCGCCGCGCGTGGAGTTTATTGCGTATACCGGGCTTT  >  CP009273/3091327‑3091625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A