Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,233,271 G→T 31.1% intergenic (+367/‑32) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,233,2710GT31.1% 450.0 / 181.7 228intergenic (+367/‑32)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base G (95/62);  new base T (38/32);  total (134/94)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 234 total aligned reads displayed.

TCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGG  >  CP009273/3233124‑3233420
                                                                                                                                                   |                                                                                                                                                     
tCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGAt                                                                                                                                                     >  1:1898714/1‑150 (MQ=255)
 cAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGAtg                                                                                                                                                    >  1:863137/1‑150 (MQ=255)
  aaaGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGAtgt                                                                                                                                                   <  1:3504900/150‑1 (MQ=255)
   aaGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGAtgtg                                                                                                                                                  >  2:2752014/1‑150 (MQ=255)
       aaTGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCATGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGac                                                                                                                                              >  1:3291067/1‑150 (MQ=255)
          gTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGacaac                                                                                                                                           >  1:4317871/1‑150 (MQ=255)
               cATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAAt                                                                                                                                      >  1:3314514/1‑150 (MQ=255)
                aTTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATg                                                                                                                                     <  2:3548377/150‑1 (MQ=255)
                aTTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATg                                                                                                                                     >  2:1691737/1‑150 (MQ=255)
                    aGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAg                                                                                                                                 <  2:2086763/150‑1 (MQ=255)
                    aGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAg                                                                                                                                 >  1:2465747/1‑150 (MQ=255)
                     gTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAgg                                                                                                                                >  2:1635263/1‑150 (MQ=255)
                      tttCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGa                                                                                                                               <  2:1837249/150‑1 (MQ=255)
                         cAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCg                                                                                                                            <  2:1422967/150‑1 (MQ=255)
                          aaaaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACTCACCAGGGATGTGCGACAACACAATGAAAGGATCGa                                                                                                                           >  2:2070545/1‑150 (MQ=255)
                           aaaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaa                                                                                                                          <  1:3069648/150‑1 (MQ=255)
                           aaaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGaa                                                                                                                          <  2:796231/150‑1 (MQ=255)
                           aaaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaa                                                                                                                          >  2:1226600/1‑150 (MQ=255)
                            aaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaa                                                                                                                         >  2:1017083/1‑150 (MQ=255)
                            aaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaa                                                                                                                         >  2:4006070/1‑150 (MQ=255)
                            aaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaa                                                                                                                         <  1:1229841/150‑1 (MQ=255)
                            aaTATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaa                                                                                                                         >  1:1607674/1‑150 (MQ=255)
                             atatGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaaa                                                                                                                        >  1:15580/1‑150 (MQ=255)
                              tatGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGaaaaa                                                                                                                       >  1:2743638/1‑150 (MQ=255)
                                   ccTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACt                                                                                                                  <  1:1665057/150‑1 (MQ=255)
                                   ccTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACt                                                                                                                  <  2:387000/150‑1 (MQ=255)
                                     tctctTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTAc                                                                                                                <  2:3592712/150‑1 (MQ=255)
                                        ctTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCa                                                                                                             <  2:3425298/150‑1 (MQ=255)
                                           tAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACg                                                                                                          >  2:4222002/1‑150 (MQ=255)
                                           tAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACg                                                                                                          >  1:3151973/1‑150 (MQ=255)
                                           tAAAATCCAGCATTTTTAGCTTCCCGAAGCTGTAACTTTACTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACg                                                                                                          <  2:381051/150‑1 (MQ=255)
                                            aaaaTCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGt                                                                                                         >  1:468454/1‑150 (MQ=255)
                                            aaaaTCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGt                                                                                                         >  2:589586/1‑150 (MQ=255)
                                              aaTCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTc                                                                                                       <  1:3397626/150‑1 (MQ=255)
                                                tCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCAc                                                                                                     <  1:1392162/150‑1 (MQ=255)
                                                tCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCAc                                                                                                     <  1:4006283/150‑1 (MQ=255)
                                                 ccAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCAcc                                                                                                    >  1:4247034/1‑150 (MQ=255)
                                                    gCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCggg                                                                                                 >  2:683736/1‑150 (MQ=255)
                                                     cATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCgggg                                                                                                >  2:514868/1‑150 (MQ=255)
                                                     cATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCgggg                                                                                                >  2:2833217/1‑150 (MQ=255)
                                                      aTTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGc                                                                                               >  1:4002612/1‑150 (MQ=255)
                                                       tttttCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGGTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCt                                                                                              <  2:1351465/150‑1 (MQ=255)
                                                           tCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTc                                                                                          >  2:2918689/1‑150 (MQ=255)
                                                           tCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTc                                                                                          >  2:4252757/1‑150 (MQ=255)
                                                             gCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCg                                                                                        <  1:2024890/150‑1 (MQ=255)
                                                             gCTTCCCGAAGCTGTAACTTTCCTTATACTCGAACTTGCACACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCg                                                                                        <  1:569914/150‑1 (MQ=255)
                                                              cTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCgg                                                                                       <  2:2193524/150‑1 (MQ=255)
                                                                tCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCg                                                                                     >  2:644608/1‑150 (MQ=255)
                                                                    gAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTg                                                                                 <  2:3032328/150‑1 (MQ=255)
                                                                    gAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGACAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTg                                                                                 <  2:185838/150‑1 (MQ=255)
                                                                    gAAGCTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTg                                                                                 <  2:2441069/150‑1 (MQ=255)
                                                                        cTGTAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTc                                                                             <  1:174541/150‑1 (MQ=255)
                                                                           tAACTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATg                                                                          <  1:1073079/150‑1 (MQ=255)
                                                                             aCTTTCCTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCGTGGc                                                                        >  2:220057/1‑150 (MQ=255)
                                                                              cTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCa                                                                       <  2:3818984/150‑1 (MQ=255)
                                                                              cTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCa                                                                       <  2:3819268/150‑1 (MQ=255)
                                                                               tttCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAg                                                                      >  1:4003913/1‑150 (MQ=255)
                                                                                  ccTTATACTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCt                                                                   <  2:2465747/150‑1 (MQ=255)
                                                                                      ataCTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTa                                                               >  1:3993013/1‑150 (MQ=255)
                                                                                      ataCTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTa                                                               >  1:2528290/1‑150 (MQ=255)
                                                                                      ataCTCGAACTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTa                                                               >  1:4228174/1‑150 (MQ=255)
                                                                                            gACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAATACaa                                                         >  2:2307083/1‑150 (MQ=255)
                                                                                               cTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATc                                                      >  2:1484213/1‑150 (MQ=255)
                                                                                                ttGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATcc                                                     >  2:3758215/1‑150 (MQ=255)
                                                                                                 tGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCt                                                    <  2:3862235/150‑1 (MQ=255)
                                                                                                 tGCAAACACTGTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCt                                                    <  2:2459101/150‑1 (MQ=255)
                                                                                                  gCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTg                                                   <  1:4630852/150‑1 (MQ=255)
                                                                                                   cAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTgg                                                  >  1:3559694/1‑150 (MQ=255)
                                                                                                    aaaCACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGt                                                 >  1:3884815/1‑150 (MQ=255)
                                                                                                     aaCACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTc                                                <  1:1988467/150‑1 (MQ=255)
                                                                                                      acacTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCg                                               >  1:947699/1‑150 (MQ=255)
                                                                                                      acacTTAGTTACATCCAGAAAGATTCGTCGACAGAACGCACCAGGGATGTGCGACAACACAAAGAAAGGAACGAAAAAAGACTACTCAACTGTCACCGCGGCTATTCCGGCGACTGGCTCAAGGCAGCCTGCTAAAAAAAATCCTGGTCg                                               >  2:4400583/1‑150 (MQ=255)
                                                                                                        acTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGc                                             >  2:3280791/1‑150 (MQ=255)
                                                                                                         cTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGcc                                            >  1:686967/1‑150 (MQ=255)
                                                                                                         cTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGcc                                            <  2:2604514/150‑1 (MQ=255)
                                                                                                         cTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGTGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGcc                                            >  2:2749218/1‑150 (MQ=255)
                                                                                                          tttGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCt                                           <  2:865734/150‑1 (MQ=255)
                                                                                                           ttGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCtt                                          <  2:4567732/150‑1 (MQ=255)
                                                                                                            tGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTg                                         <  1:72568/150‑1 (MQ=255)
                                                                                                            tGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTg                                         <  1:1574548/150‑1 (MQ=255)
                                                                                                              ttACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGtt                                       >  2:4152788/1‑150 (MQ=255)
                                                                                                              ttACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGtt                                       >  2:1097874/1‑150 (MQ=255)
                                                                                                                aCATCCTGAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCt                                     >  1:3246631/1‑150 (MQ=255)
                                                                                                                  aTCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTgg                                   >  2:2030435/1‑150 (MQ=255)
                                                                                                                       gAAAGATGCGTCGACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGAtt                              >  1:1946245/1‑150 (MQ=255)
                                                                                                                          aGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGAttctt                           <  2:1709127/150‑1 (MQ=255)
                                                                                                                            aTGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATtcttct                         <  2:2817021/150‑1 (MQ=255)
                                                                                                                             tGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTg                        <  1:1715436/150‑1 (MQ=255)
                                                                                                                                   gACAGAACGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATgg                  >  2:3303362/1‑150 (MQ=255)
                                                                                                                                    aCAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGa                 <  1:2485595/150‑1 (MQ=255)
                                                                                                                                    aCAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGa                 <  1:2357198/150‑1 (MQ=255)
                                                                                                                                        aaCGCACCAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGAtctc             >  1:1710688/1‑150 (MQ=255)
                                                                                                                                           gCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCaaa          >  1:2126710/1‑150 (MQ=255)
                                                                                                                                             aCCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAAc        >  2:194898/1‑150 (MQ=255)
                                                                                                                                             aCCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAAc        >  2:1735776/1‑150 (MQ=255)
                                                                                                                                              ccAGGTATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAAcc       >  1:2217077/1‑150 (MQ=255)
                                                                                                                                                 gggATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCcgc    >  2:4036909/1‑150 (MQ=255)
                                                                                                                                                  ggATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCcgcg   >  1:3741735/1‑150 (MQ=255)
                                                                                                                                                  ggATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCcgcg   >  2:945633/1‑150 (MQ=255)
                                                                                                                                                   gATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCgcgg  >  1:2024144/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGTTACATCCTGAAAGATGCGTCGACAGAACGCACCAGGGATGTGCGACAACACAATGAAAGGATCGAAAAATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGG  >  CP009273/3233124‑3233420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A