Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,495,516 C→A 50.0% S297* (TCG→TAG)  frlB → fructoselysine‑6‑P‑deglycase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,495,5160CA50.0% 121.9 / inf 254S297* (TCG→TAG) frlBfructoselysine‑6‑P‑deglycase
Reads supporting (aligned to +/- strand):  ref base C (69/58);  new base A (60/67);  total (129/125)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

CCGCCATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCG  >  CP009273/3495367‑3495665
                                                                                                                                                     |                                                                                                                                                     
ccGCCATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCATCTCGGCAATGATGAAAGTCGCCACACCACCGAACCCGCCATTAACTTTGTTAAACAGCGAACTGACAACGTGATCGTCATCGATTACGCCGAAATTTc                                                                                                                                                       >  1:2956376/1‑150 (MQ=255)
  gCCATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGc                                                                                                                                                     >  2:116155/1‑150 (MQ=255)
   ccATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCa                                                                                                                                                    >  1:3248622/1‑150 (MQ=255)
   ccATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCa                                                                                                                                                    >  2:954057/1‑150 (MQ=255)
   ccATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCa                                                                                                                                                    <  2:2421442/150‑1 (MQ=255)
    cATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCaa                                                                                                                                                   >  1:2088426/1‑150 (MQ=255)
    cATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCaa                                                                                                                                                   <  1:1366265/150‑1 (MQ=255)
    cATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCaa                                                                                                                                                   <  2:231795/150‑1 (MQ=255)
      tGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAgg                                                                                                                                                 >  1:852477/1‑150 (MQ=255)
       ggCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAggg                                                                                                                                                <  1:2113795/150‑1 (MQ=255)
        gCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGc                                                                                                                                               <  1:1849187/150‑1 (MQ=255)
          ccGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTg                                                                                                                                             <  1:1464983/150‑1 (MQ=255)
            gCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCa                                                                                                                                           <  2:1227999/150‑1 (MQ=255)
            gCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCa                                                                                                                                           <  2:1593180/150‑1 (MQ=255)
            gCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCa                                                                                                                                           <  1:1292638/150‑1 (MQ=255)
            gCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGGTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCa                                                                                                                                           <  1:105540/150‑1 (MQ=255)
            gCTGGAGATTGTCGAACCGGGCGGTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCa                                                                                                                                           <  2:4499748/150‑1 (MQ=255)
             cTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCAc                                                                                                                                          <  1:3086834/150‑1 (MQ=255)
                     tGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGtggct                                                                                                                                  <  2:3122725/150‑1 (MQ=255)
                     tGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGtggct                                                                                                                                  <  2:2445139/150‑1 (MQ=255)
                        cGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGtggctggc                                                                                                                               >  1:541772/1‑150 (MQ=255)
                         gAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCa                                                                                                                              >  1:3886178/1‑150 (MQ=255)
                         gAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCa                                                                                                                              >  1:3886191/1‑150 (MQ=255)
                           aCCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCAcc                                                                                                                            <  1:1420116/150‑1 (MQ=255)
                               ggCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTc                                                                                                                        <  2:521414/150‑1 (MQ=255)
                                  gttccgttccTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTg                                                                                                                     <  2:3193654/150‑1 (MQ=255)
                                          cctgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGt                                                                                                             <  2:953439/150‑1 (MQ=255)
                                            tgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGc                                                                                                           >  2:4339771/1‑150 (MQ=255)
                                            tgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGc                                                                                                           <  2:1513468/150‑1 (MQ=255)
                                              ttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCa                                                                                                         <  2:2074845/150‑1 (MQ=255)
                                                 ctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCTCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATg                                                                                                      >  1:4342761/1‑150 (MQ=255)
                                                 ctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATg                                                                                                      >  1:287991/1‑150 (MQ=255)
                                                  tgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATgg                                                                                                     <  1:3373023/150‑1 (MQ=255)
                                                        gCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGc                                                                                               <  2:3485902/150‑1 (MQ=255)
                                                         cAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGct                                                                                              <  2:2259037/150‑1 (MQ=255)
                                                          aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                             <  2:623321/150‑1 (MQ=255)
                                                          aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                             <  2:4101228/150‑1 (MQ=255)
                                                          aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                             <  2:3502192/150‑1 (MQ=255)
                                                           atgatgAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctct                                                                                            <  2:194558/150‑1 (MQ=255)
                                                              atgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGct                                                                                         >  1:3965965/1‑150 (MQ=255)
                                                              atgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGct                                                                                         >  1:3966557/1‑150 (MQ=255)
                                                              atgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGct                                                                                         >  2:2568213/1‑150 (MQ=255)
                                                                    gTCGCCACACCACCGAACTCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTAcc                                                                                   <  2:698026/150‑1 (MQ=255)
                                                                    gTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTAcc                                                                                   <  2:1995534/150‑1 (MQ=255)
                                                                    gTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTAcc                                                                                   <  1:1791299/150‑1 (MQ=255)
                                                                     tCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCt                                                                                  <  1:4436567/150‑1 (MQ=255)
                                                                     tCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCGACTACCt                                                                                  >  1:1766292/1‑150 (MQ=255)
                                                                       gCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCGGCTACTCCCGGt                                                                                >  1:3114489/1‑150 (MQ=255)
                                                                       gCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGt                                                                                >  1:3480250/1‑150 (MQ=255)
                                                                           caccacCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTAt                                                                            <  1:473870/150‑1 (MQ=255)
                                                                             ccaccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTAttt                                                                          <  2:2907463/150‑1 (MQ=255)
                                                                                ccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACa                                                                       <  1:332934/150‑1 (MQ=255)
                                                                                 cGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                      >  1:2200538/1‑150 (MQ=255)
                                                                                   aaCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAg                                                                    <  1:1482569/150‑1 (MQ=255)
                                                                                    aCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGa                                                                   <  1:2364265/150‑1 (MQ=255)
                                                                                     cgcgCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGAt                                                                  <  2:1909252/150‑1 (MQ=255)
                                                                                      gcgcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATc                                                                 <  1:2352199/150‑1 (MQ=255)
                                                                                       cgcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATca                                                                <  2:2004875/150‑1 (MQ=255)
                                                                                        gcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcac                                                               >  1:4585758/1‑150 (MQ=255)
                                                                                        gcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcac                                                               >  1:4183380/1‑150 (MQ=255)
                                                                                        gcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcac                                                               <  1:1871964/150‑1 (MQ=255)
                                                                                          cATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACaa                                                             >  2:3101574/1‑150 (MQ=255)
                                                                                               aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                        <  2:383926/150‑1 (MQ=255)
                                                                                               aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                        >  2:1679432/1‑150 (MQ=255)
                                                                                               aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                        >  1:2296449/1‑150 (MQ=255)
                                                                                               aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                        <  2:3014999/150‑1 (MQ=255)
                                                                                                   tGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                    >  1:3624973/1‑150 (MQ=255)
                                                                                                     ttAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAc                                                  >  1:262237/1‑150 (MQ=255)
                                                                                                      tAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcg                                                 <  2:2528783/150‑1 (MQ=255)
                                                                                                       aaaCAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgc                                                >  2:1291544/1‑150 (MQ=255)
                                                                                                             cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTAt                                          >  1:2656141/1‑150 (MQ=255)
                                                                                                               tACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTa                                        <  2:1833814/150‑1 (MQ=255)
                                                                                                                aCTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTAc                                       >  2:1447574/1‑150 (MQ=255)
                                                                                                                aCTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTAc                                       <  2:2693425/150‑1 (MQ=255)
                                                                                                                   gACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACggt                                    >  2:2669934/1‑150 (MQ=255)
                                                                                                                    aCAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACggtg                                   >  1:1669943/1‑150 (MQ=255)
                                                                                                                     cAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACggtgg                                  >  1:24970/1‑150 (MQ=255)
                                                                                                                      aaCGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACggtggt                                 >  1:3658179/1‑150 (MQ=255)
                                                                                                                        cGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                               >  1:3162506/1‑150 (MQ=255)
                                                                                                                        cGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                               >  2:3544896/1‑150 (MQ=255)
                                                                                                                        cGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                               >  2:3678351/1‑150 (MQ=255)
                                                                                                                         gTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCtg                              <  2:89248/150‑1 (MQ=255)
                                                                                                                             tCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCtggtgg                          >  1:2212970/1‑150 (MQ=255)
                                                                                                                                  aTCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAatat                     <  2:2972731/150‑1 (MQ=255)
                                                                                                                                    cGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTa                   >  2:362798/1‑150 (MQ=255)
                                                                                                                                        tACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATcc               <  1:2737378/150‑1 (MQ=255)
                                                                                                                                          cGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCct             >  2:2326061/1‑150 (MQ=255)
                                                                                                                                          cGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCct             <  1:1116470/150‑1 (MQ=255)
                                                                                                                                          cGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCct             <  1:604694/150‑1 (MQ=255)
                                                                                                                                             cGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCtctc          >  2:3984464/1‑150 (MQ=255)
                                                                                                                                             cGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCtctc          >  2:495033/1‑150 (MQ=255)
                                                                                                                                             cGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCtctc          >  1:1541729/1‑150 (MQ=255)
                                                                                                                                              gAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcc         >  2:4439593/1‑150 (MQ=255)
                                                                                                                                              gAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcc         >  1:71040/1‑150 (MQ=255)
                                                                                                                                              gAAATTTAGCAAGGGCTGCACCCGTGGCGGGCACCGTTCCTGAGGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcc         <  1:2791921/150‑1 (MQ=255)
                                                                                                                                               aaaTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTccc        >  2:3060439/1‑150 (MQ=255)
                                                                                                                                               aaaTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTccc        <  2:2091414/150‑1 (MQ=255)
                                                                                                                                                aaTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccg       <  2:753209/150‑1 (MQ=255)
                                                                                                                                                 aTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTACTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccgg      >  2:1715719/1‑150 (MQ=255)
                                                                                                                                                 aTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTACTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccgg      >  2:1715668/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGCCATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCG  >  CP009273/3495367‑3495665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A