Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,495,516 C→A 50.9% S297* (TCG→TAG)  frlB → fructoselysine‑6‑P‑deglycase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,495,5160CA50.9% 82.4 / inf 239S297* (TCG→TAG) frlBfructoselysine‑6‑P‑deglycase
Reads supporting (aligned to +/- strand):  ref base C (52/65);  new base A (52/70);  total (104/135)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

Only 100 of 239 total aligned reads displayed.

CATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCC  >  CP009273/3495371‑3495664
                                                                                                                                                 |                                                                                                                                                    
cATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCaa                                                                                                                                                  >  2:3838541/1‑150 (MQ=255)
cATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCaa                                                                                                                                                  <  2:2603460/150‑1 (MQ=255)
 aTGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAg                                                                                                                                                 <  2:1622213/150‑1 (MQ=255)
 aTGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAg                                                                                                                                                 <  2:866895/150‑1 (MQ=255)
 aTGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAg                                                                                                                                                 <  2:4232643/150‑1 (MQ=255)
  tGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAgg                                                                                                                                                >  1:2659830/1‑150 (MQ=255)
   ggCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAggg                                                                                                                                               <  2:3816712/150‑1 (MQ=255)
   ggCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATAGTCATCGATTACGCCGAAATTTAGCAAggg                                                                                                                                               <  2:3816693/150‑1 (MQ=255)
    gCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGc                                                                                                                                              >  1:2651963/1‑150 (MQ=255)
     cccGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCt                                                                                                                                             >  2:761063/1‑150 (MQ=255)
         cTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCAc                                                                                                                                         <  2:3987629/150‑1 (MQ=255)
         cTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCAc                                                                                                                                         <  1:995074/150‑1 (MQ=255)
          tGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCAcc                                                                                                                                        >  1:1976354/1‑150 (MQ=255)
            gagaTTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCg                                                                                                                                      >  2:11790/1‑150 (MQ=255)
            gagaTTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCg                                                                                                                                      >  1:2816327/1‑150 (MQ=255)
              gaTTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGtg                                                                                                                                    >  2:2399416/1‑150 (MQ=255)
                  gTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGtggctg                                                                                                                                <  1:3772009/150‑1 (MQ=255)
                          gggCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGtt                                                                                                                        >  2:3728134/1‑150 (MQ=255)
                                tccgttccTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAGTTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGAt                                                                                                                  >  1:4218217/1‑150 (MQ=255)
                                    ttcctgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTc                                                                                                              <  2:3452973/150‑1 (MQ=255)
                                      cctgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCATCGTTCCTGATGTTCGt                                                                                                            >  1:769761/1‑150 (MQ=255)
                                       ctgttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTg                                                                                                           <  2:3057013/150‑1 (MQ=255)
                                          ttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCa                                                                                                        <  1:220387/150‑1 (MQ=255)
                                          ttcctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCa                                                                                                        <  1:1931181/150‑1 (MQ=255)
                                            cctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAAt                                                                                                      >  1:1775505/1‑150 (MQ=255)
                                            cctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAAt                                                                                                      <  1:1474686/150‑1 (MQ=255)
                                             ctgCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATg                                                                                                     <  2:3304542/150‑1 (MQ=255)
                                                cTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAg                                                                                                  >  2:806031/1‑150 (MQ=255)
                                                 tCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGt                                                                                                 >  2:884732/1‑150 (MQ=255)
                                                 tCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGt                                                                                                 <  2:306603/150‑1 (MQ=255)
                                                 tCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGt                                                                                                 >  1:1352411/1‑150 (MQ=255)
                                                   ggCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTgg                                                                                               >  2:4467803/1‑150 (MQ=255)
                                                    gCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGc                                                                                              <  1:3147044/150‑1 (MQ=255)
                                                    gCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGc                                                                                              <  1:2630754/150‑1 (MQ=255)
                                                    gCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGc                                                                                              <  1:337923/150‑1 (MQ=255)
                                                      aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGGGTGGctc                                                                                            >  1:161471/1‑150 (MQ=255)
                                                      aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                            <  1:499475/150‑1 (MQ=255)
                                                      aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                            >  1:2463468/1‑150 (MQ=255)
                                                      aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                            >  2:3450845/1‑150 (MQ=255)
                                                      aaTGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGctc                                                                                            >  2:1178266/1‑150 (MQ=255)
                                                        tgatgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTg                                                                                          >  1:579999/1‑150 (MQ=255)
                                                        tgatgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTg                                                                                          >  1:580338/1‑150 (MQ=255)
                                                           tgaAAGTCGCCACACCACCGACCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGcta                                                                                       <  2:492910/150‑1 (MQ=255)
                                                            gaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGctac                                                                                      >  1:511368/1‑150 (MQ=255)
                                                             aaaGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGctact                                                                                     <  1:1991341/150‑1 (MQ=255)
                                                                 tCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCt                                                                                 <  1:2988093/150‑1 (MQ=255)
                                                                        accaccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTAtt                                                                          <  2:1571842/150‑1 (MQ=255)
                                                                        accaccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTAtt                                                                          >  2:1453992/1‑150 (MQ=255)
                                                                        accaccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTAtt                                                                          >  1:278302/1‑150 (MQ=255)
                                                                          caccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTa                                                                        >  2:2746483/1‑150 (MQ=255)
                                                                          caccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTa                                                                        <  2:1805441/150‑1 (MQ=255)
                                                                           accGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTAc                                                                       >  1:4010399/1‑150 (MQ=255)
                                                                           accGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTAc                                                                       >  1:428357/1‑150 (MQ=255)
                                                                             cGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                     >  2:3201459/1‑150 (MQ=255)
                                                                             cGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTCACaa                                                                     >  2:3202151/1‑150 (MQ=255)
                                                                               aaCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAg                                                                   <  2:1975138/150‑1 (MQ=255)
                                                                                   cgcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATca                                                               >  1:4357062/1‑150 (MQ=255)
                                                                                    gcCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcac                                                              <  2:4216632/150‑1 (MQ=255)
                                                                                     ccATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcaca                                                             <  1:2449899/150‑1 (MQ=255)
                                                                                     ccATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATcaca                                                             >  1:3260579/1‑150 (MQ=255)
                                                                                      cATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACaa                                                            <  2:1983773/150‑1 (MQ=255)
                                                                                       aTTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAAc                                                           <  2:663222/150‑1 (MQ=255)
                                                                                        ttAACTTTGTTAAACAGCGTACTTACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAAcc                                                          <  2:120702/150‑1 (MQ=255)
                                                                                           aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                       >  1:4495006/1‑150 (MQ=255)
                                                                                           aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                       >  2:1644037/1‑150 (MQ=255)
                                                                                           aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                       >  2:3903429/1‑150 (MQ=255)
                                                                                           aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCgg                                                       >  2:1016966/1‑150 (MQ=255)
                                                                                             tttGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCACTGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGAt                                                     >  2:3327461/1‑150 (MQ=255)
                                                                                             tttGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGAt                                                     >  2:3328863/1‑150 (MQ=255)
                                                                                              ttGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATg                                                    >  1:1422086/1‑150 (MQ=255)
                                                                                               tGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                   <  2:2881025/150‑1 (MQ=255)
                                                                                               tGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                   <  2:594886/150‑1 (MQ=255)
                                                                                               tGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                   >  1:2183166/1‑150 (MQ=255)
                                                                                                 ttAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAc                                                 <  2:1185036/150‑1 (MQ=255)
                                                                                                   aaaCAGCGTACTGACAACGTGATAGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgc                                               >  2:3176608/1‑150 (MQ=255)
                                                                                                     aCAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccg                                             >  2:3153124/1‑150 (MQ=255)
                                                                                                        gCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                          <  1:1144125/150‑1 (MQ=255)
                                                                                                        gCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                          <  1:4087709/150‑1 (MQ=255)
                                                                                                        gCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                          <  1:3913947/150‑1 (MQ=255)
                                                                                                        gCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                          <  1:4086443/150‑1 (MQ=255)
                                                                                                          gTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTACCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTAtt                                        <  1:2930894/150‑1 (MQ=255)
                                                                                                          gTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGATTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTAtt                                        <  2:568651/150‑1 (MQ=255)
                                                                                                             cTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACg                                     <  2:1246388/150‑1 (MQ=255)
                                                                                                               gACAACGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACggt                                   >  2:357106/1‑150 (MQ=255)
                                                                                                                    cGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                              <  2:1352411/150‑1 (MQ=255)
                                                                                                                    cGTGATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                              <  2:2393011/150‑1 (MQ=255)
                                                                                                                       gATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCtggt                           >  2:560742/1‑150 (MQ=255)
                                                                                                                       gATCGTCATCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCtggt                           <  2:2041289/150‑1 (MQ=255)
                                                                                                                            tCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAat                      <  2:2463468/150‑1 (MQ=255)
                                                                                                                              aTCGATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAatat                    <  1:1531092/150‑1 (MQ=255)
                                                                                                                               tCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATAtt                   >  1:2631521/1‑150 (MQ=255)
                                                                                                                                cGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTa                  <  2:2969293/150‑1 (MQ=255)
                                                                                                                                 gATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTaa                 >  2:4501143/1‑150 (MQ=255)
                                                                                                                                 gATTACGCCGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTaa                 <  2:1863741/150‑1 (MQ=255)
                                                                                                                                    tACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATcc              >  2:2646256/1‑150 (MQ=255)
                                                                                                                                         cGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCtctc         <  2:916182/150‑1 (MQ=255)
                                                                                                                                         cGAAATTTAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCtctc         <  2:4049052/150‑1 (MQ=255)
                                                                                                                                            aaTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccg      >  1:489766/1‑150 (MQ=255)
                                                                                                                                             aTTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccgg     <  1:4505539/150‑1 (MQ=255)
                                                                                                                                              tttAGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccggc    >  1:1714565/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CATGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCC  >  CP009273/3495371‑3495664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A