Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,087,476 A→T 8.9% intergenic (‑126/‑188) rhaD ← / → rhaS pseudogene, truncated, rhamnulose‑phosphate aldolase/transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,087,4760AT 8.9% 872.2 / 58.0 270intergenic (‑126/‑188)rhaD/rhaSpseudogene, truncated, rhamnulose‑phosphate aldolase/transcriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base A (121/125);  new base T (5/19);  total (126/144)
Fisher's exact test for biased strand distribution p-value = 9.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 280 total aligned reads displayed.

TAGCCCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGA  >  CP009273/4087328‑4087625
                                                                                                                                                    |                                                                                                                                                     
tAGCCCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGaa                                                                                                                                                      <  1:298273/150‑1 (MQ=255)
 aGCCCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGaaa                                                                                                                                                     <  2:430677/150‑1 (MQ=255)
  gCCCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAg                                                                                                                                                    <  1:4436936/150‑1 (MQ=255)
     cgcgCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATg                                                                                                                                                 <  2:2594963/150‑1 (MQ=255)
     cgcgCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATg                                                                                                                                                 <  2:4463949/150‑1 (MQ=255)
      gcgcCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGa                                                                                                                                                <  1:328965/150‑1 (MQ=255)
       cgcCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGaa                                                                                                                                               >  1:2699921/1‑150 (MQ=255)
        gcCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAAc                                                                                                                                              <  1:871887/150‑1 (MQ=255)
             cGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGtgat                                                                                                                                         >  1:2660758/1‑150 (MQ=255)
             cGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                                                                                                         <  2:840068/150‑1 (MQ=255)
             cGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                                                                                                         >  1:972990/1‑150 (MQ=255)
             cGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                                                                                                         <  2:2927309/150‑1 (MQ=255)
             cGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtgat                                                                                                                                         <  1:3095026/150‑1 (MQ=255)
              gCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtgatg                                                                                                                                        <  1:500026/150‑1 (MQ=255)
               cTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATGGGCAACCAGGGAAAGATGAACGtgatga                                                                                                                                       <  1:1802418/150‑1 (MQ=255)
                 gTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGatgatg                                                                                                                                     >  1:2681577/1‑150 (MQ=255)
                   cGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                                                                                                   >  1:173942/1‑150 (MQ=255)
                   cGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                                                                                                   <  2:3714502/150‑1 (MQ=255)
                   cGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtc                                                                                                                                   <  2:3948208/150‑2 (MQ=255)
                    gACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                                                                                                  <  2:2193312/150‑1 (MQ=255)
                     aCGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTca                                                                                                                                 <  1:1610508/150‑1 (MQ=255)
                            cGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                                                                                                          <  2:1615407/150‑1 (MQ=255)
                            cGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                                                                                                          >  1:2159793/1‑150 (MQ=255)
                             ggATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGc                                                                                                                         <  2:3782920/150‑1 (MQ=255)
                              gATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCt                                                                                                                        <  1:296698/150‑1 (MQ=255)
                              gATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCt                                                                                                                        >  1:2126209/1‑150 (MQ=255)
                                tCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGa                                                                                                                      <  2:2738250/150‑1 (MQ=255)
                                 ccTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACCGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGaa                                                                                                                     >  2:2295651/1‑150 (MQ=255)
                                    ctAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtg                                                                                                                  <  1:319727/150‑1 (MQ=255)
                                      agagTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgtg                                                                                                                >  1:2805892/1‑150 (MQ=255)
                                      agagTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgtg                                                                                                                >  1:4637595/1‑150 (MQ=255)
                                        agTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggt                                                                                                              >  1:3481555/1‑150 (MQ=255)
                                           cGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                                                                                                           <  2:1997043/150‑1 (MQ=255)
                                            gACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGACAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatg                                                                                                          >  2:796019/1‑150 (MQ=255)
                                             aCATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgt                                                                                                         >  1:403919/1‑150 (MQ=255)
                                               atatGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                                                                                                       >  1:3130581/1‑150 (MQ=255)
                                                    gTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTc                                                                                                  >  2:270452/1‑150 (MQ=255)
                                                       aTCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAcc                                                                                               <  2:3507482/150‑1 (MQ=255)
                                                          ctgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCa                                                                                            <  2:972990/150‑1 (MQ=255)
                                                           tgctgAATTTCATTACGACCAGTCTCAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           >  2:2520685/1‑150 (MQ=255)
                                                           tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           >  2:729246/1‑150 (MQ=255)
                                                           tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           <  2:4643785/150‑1 (MQ=255)
                                                                aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      <  1:1266002/150‑1 (MQ=255)
                                                                aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      >  1:3755374/1‑150 (MQ=255)
                                                                aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      <  2:1068566/150‑1 (MQ=255)
                                                                 aTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg                                                                                     <  1:2317714/150‑1 (MQ=255)
                                                                  tttCATAACGACCAGGCTAAACAGCGCCTGAATGCGCGACCTTCTCGTTACACACAGGACAATGGGCCATTGGCAACCACGGAAAGATGAACGTTATGATGTTCACAATTTGCTGAATTGTGGAGATGTGATGCTCACCGCATTTCCTGa                                                                                    >  2:2156178/1‑150 (MQ=255)
                                                                  tttCAGTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGa                                                                                    <  2:2353187/150‑1 (MQ=255)
                                                                    tCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaa                                                                                  >  1:3933301/1‑150 (MQ=255)
                                                                    tCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGaaa                                                                                  >  1:1623755/1‑150 (MQ=255)
                                                                       ttACGACCAGTCTAAAAAGCGCCTGACTTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAtt                                                                               <  1:75848/150‑1 (MQ=255)
                                                                       ttACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAtt                                                                               >  2:2459196/1‑150 (MQ=255)
                                                                        tACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAATGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTc                                                                              <  2:1724767/150‑1 (MQ=255)
                                                                        tACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTc                                                                              <  2:2075439/150‑1 (MQ=255)
                                                                           gACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACg                                                                           >  2:53662/1‑150 (MQ=255)
                                                                             ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCt                                                                         <  1:2143955/150‑1 (MQ=255)
                                                                                 tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTAt                                                                     >  1:3448869/1‑150 (MQ=255)
                                                                                 tCTAAAAAGCGCCTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTAt                                                                     <  1:1542452/150‑1 (MQ=255)
                                                                                    aaaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCtt                                                                  <  2:609221/150‑1 (MQ=255)
                                                                                    aaaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCtt                                                                  >  1:1083906/1‑150 (MQ=255)
                                                                                    aaaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCtt                                                                  <  1:2049409/150‑1 (MQ=255)
                                                                                     aaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTg                                                                 >  1:4165727/1‑150 (MQ=255)
                                                                                      aaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGa                                                                >  2:3582785/1‑150 (MQ=255)
                                                                                       aaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGaa                                                               <  1:3349559/150‑1 (MQ=255)
                                                                                          cgcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGaaaaa                                                            >  1:2754141/1‑150 (MQ=255)
                                                                                            ccTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATc                                                          <  1:3834158/150‑1 (MQ=255)
                                                                                             cTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCg                                                         <  2:1326368/150‑1 (MQ=255)
                                                                                             cTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCg                                                         <  1:2955745/150‑1 (MQ=255)
                                                                                             cTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCg                                                         <  1:2955795/150‑1 (MQ=255)
                                                                                                 aTTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGt                                                     >  2:29545/1‑150 (MQ=255)
                                                                                                   tCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGttt                                                   <  2:899674/150‑1 (MQ=255)
                                                                                                    cgcgACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGtttt                                                  <  2:271377/150‑1 (MQ=255)
                                                                                                       gACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATTCTCACCGCATTTCCTGAAAATTAACGCTGTATCTTGAAAAATCGACGTTTTTTa                                               >  2:2852998/1‑150 (MQ=255)
                                                                                                       gACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTa                                               >  1:3658155/1‑150 (MQ=255)
                                                                                                       gACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTa                                               >  1:3183509/1‑150 (MQ=255)
                                                                                                        aCCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTAc                                              <  1:4611228/150‑1 (MQ=255)
                                                                                                         ccTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACg                                             >  2:4492954/1‑150 (MQ=255)
                                                                                                          cTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGt                                            <  1:4319032/150‑1 (MQ=255)
                                                                                                           ttCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTg                                           >  1:1143339/1‑150 (MQ=255)
                                                                                                              tcGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGtt                                        <  2:3098028/150‑1 (MQ=255)
                                                                                                              tcGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGtt                                        <  2:2693563/150‑1 (MQ=255)
                                                                                                               cGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGttt                                       <  1:1399159/150‑1 (MQ=255)
                                                                                                                gTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGtttt                                      <  2:1793026/150‑1 (MQ=255)
                                                                                                                  tACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGttttac                                    >  2:2253753/1‑148 (MQ=255)
                                                                                                                     tGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTc                                 <  2:124392/150‑1 (MQ=255)
                                                                                                                     tGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTc                                 <  2:2502050/150‑1 (MQ=255)
                                                                                                                         aGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGaaa                             >  2:4412088/1‑150 (MQ=255)
                                                                                                                            aaaaTGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAAtt                          <  1:2282731/150‑1 (MQ=255)
                                                                                                                             aaaTGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAAttt                         <  2:1876615/150‑1 (MQ=255)
                                                                                                                              aaTGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTa                        >  2:4240614/1‑150 (MQ=255)
                                                                                                                                tGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAg                      >  2:1677307/1‑150 (MQ=255)
                                                                                                                                 gggCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAgg                     >  2:1159934/1‑150 (MQ=255)
                                                                                                                                 gggCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAgg                     >  2:289449/1‑150 (MQ=255)
                                                                                                                                  ggCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGt                    >  2:3839349/1‑150 (MQ=255)
                                                                                                                                      aTTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGa                <  2:182474/150‑1 (MQ=255)
                                                                                                                                         ggCAACCAGGGAAAGATGATCGTGATGATGTTAACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAATATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAAcc             <  2:3426974/150‑1 (MQ=255)
                                                                                                                                          gCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGGCGAAAATTTAAGGTAAGAACCt            >  2:1414591/1‑150 (MQ=255)
                                                                                                                                            aaCCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGa          >  2:2539182/1‑150 (MQ=255)
                                                                                                                                            aaCCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTACGTCGAAAATTTAAGGTAAGAACCAGa          >  2:1187858/1‑150 (MQ=255)
                                                                                                                                                aGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTc      <  2:3273107/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAGCCCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGA  >  CP009273/4087328‑4087625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A