Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,087,476 A→T 36.4% intergenic (‑126/‑188) rhaD ← / → rhaS pseudogene, truncated, rhamnulose‑phosphate aldolase/transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,087,4760AT36.4% 312.7 / inf 280intergenic (‑126/‑188)rhaD/rhaSpseudogene, truncated, rhamnulose‑phosphate aldolase/transcriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base A (84/94);  new base T (48/54);  total (132/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGA  >  CP009273/4087332‑4087625
                                                                                                                                                |                                                                                                                                                     
ccGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGAt                                                                                                                                                  <  2:3912070/150‑1 (MQ=255)
ccGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTGCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGAt                                                                                                                                                  <  2:3178935/150‑1 (MQ=255)
  gcgcCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGa                                                                                                                                                <  2:279600/150‑1 (MQ=255)
   cgcCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGaa                                                                                                                                               >  2:186693/1‑150 (MQ=255)
       gTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGtg                                                                                                                                           <  1:1120560/150‑1 (MQ=255)
               cGACGCATGCGGATCCTCTAGAGTCGACAAATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTAGTTACTGACATGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGtt                                                                                                                                   >  1:415076/1‑150 (MQ=255)
                gACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTc                                                                                                                                  <  1:1483520/150‑1 (MQ=255)
                   gCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTcaca                                                                                                                               <  1:1276363/150‑1 (MQ=255)
                    cATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACaa                                                                                                                              >  2:1592872/1‑150 (MQ=255)
                        cGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTg                                                                                                                          <  1:1407021/150‑1 (MQ=255)
                        cGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTg                                                                                                                          <  2:127880/150‑1 (MQ=255)
                            tCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGa                                                                                                                      <  1:1435574/150‑1 (MQ=255)
                               tctAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAAtt                                                                                                                   <  2:1416605/150‑1 (MQ=255)
                                ctAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCAAAATTTGCTGAATtg                                                                                                                  >  1:285990/1‑150 (MQ=255)
                                 tAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                                                                                                 <  1:2590313/150‑1 (MQ=255)
                                 tAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgt                                                                                                                 <  1:2919883/150‑1 (MQ=255)
                                  agagTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATtgtg                                                                                                                >  1:1599523/1‑150 (MQ=255)
                                   gagTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtgg                                                                                                               <  2:702929/150‑1 (MQ=255)
                                   gagTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCGTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtgg                                                                                                               <  2:1523073/150‑1 (MQ=255)
                                    agTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggt                                                                                                              >  2:312496/1‑150 (MQ=255)
                                    agTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggt                                                                                                              >  1:1641045/1‑150 (MQ=255)
                                     gTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTgtggtg                                                                                                             <  1:4069215/150‑1 (MQ=255)
                                       cGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                                                                                                           >  2:3419197/1‑150 (MQ=255)
                                       cGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                                                                                                           >  2:585900/1‑150 (MQ=255)
                                       cGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgat                                                                                                           >  1:1967823/1‑150 (MQ=255)
                                           atatGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGgtgatgtga                                                                                                       >  2:1294753/1‑150 (MQ=255)
                                             atGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGtgatgtgatg                                                                                                     <  1:2235561/150‑1 (MQ=255)
                                                 tGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCa                                                                                                 <  1:916657/150‑1 (MQ=255)
                                                  gATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAc                                                                                                >  1:2673367/1‑150 (MQ=255)
                                                   aTCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCAcc                                                                                               <  1:2421360/150‑1 (MQ=255)
                                                     ccTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGc                                                                                             >  1:2623982/1‑150 (MQ=255)
                                                       tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           >  1:3290575/1‑150 (MQ=255)
                                                       tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           <  1:596129/150‑1 (MQ=255)
                                                       tgctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGGTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAt                                                                                           <  2:434784/150‑1 (MQ=255)
                                                        gctgAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCAtt                                                                                          <  2:177371/150‑1 (MQ=255)
                                                           gAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATGTcc                                                                                       >  1:7435/1‑150 (MQ=255)
                                                            aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      <  1:544496/150‑1 (MQ=255)
                                                            aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      >  2:2711438/1‑150 (MQ=255)
                                                            aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      >  1:3626498/1‑150 (MQ=255)
                                                            aaTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCt                                                                                      >  1:726447/1‑150 (MQ=255)
                                                             aTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg                                                                                     <  1:3134178/150‑1 (MQ=255)
                                                             aTTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTg                                                                                     <  1:2277555/150‑1 (MQ=255)
                                                                  aTTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt                                                                                >  2:1707578/1‑150 (MQ=255)
                                                                  aTTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAAt                                                                                >  2:61656/1‑150 (MQ=255)
                                                                    tACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTc                                                                              >  1:2217471/1‑150 (MQ=255)
                                                                    tACGACCAGTCTAAAAAGCGCCTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTc                                                                              <  2:2255649/150‑1 (MQ=255)
                                                                       gACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACg                                                                           >  2:808110/1‑150 (MQ=255)
                                                                       gACCAGTCTAAAAAGCGCCTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACg                                                                           <  2:3690532/150‑1 (MQ=255)
                                                                         ccAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCt                                                                         >  2:667568/1‑150 (MQ=255)
                                                                           aGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGt                                                                       >  1:829714/1‑150 (MQ=255)
                                                                           aGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGt                                                                       <  1:3730178/150‑1 (MQ=255)
                                                                           aGTCTAAAAAGCGCCTGAAGTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGt                                                                       <  1:2748004/150‑1 (MQ=255)
                                                                            gTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTa                                                                      >  2:2526389/1‑150 (MQ=255)
                                                                             tCTAAAAATCGCCTGATCTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTAt                                                                     <  1:642248/150‑1 (MQ=255)
                                                                             tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTAt                                                                     <  1:2727115/150‑1 (MQ=255)
                                                                             tCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTAt                                                                     <  2:1038096/150‑1 (MQ=255)
                                                                                 aaaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTg                                                                 >  2:3538995/1‑150 (MQ=255)
                                                                                  aaaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGa                                                                >  1:3462054/1‑150 (MQ=255)
                                                                                   aaGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGaa                                                               <  2:1983121/150‑1 (MQ=255)
                                                                                    aGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGaaa                                                              >  1:475472/1‑150 (MQ=255)
                                                                                    aGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGaaa                                                              >  2:3434050/1‑150 (MQ=255)
                                                                                       gcCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAAt                                                           <  2:549105/150‑1 (MQ=255)
                                                                                           gAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTNAAAATTCACGCTGTATCTTGAAAAATCGAc                                                       <  1:3851810/150‑1 (MQ=255)
                                                                                           gAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGAc                                                       <  1:1850551/150‑1 (MQ=255)
                                                                                            aaTTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACg                                                      <  1:448851/150‑1 (MQ=255)
                                                                                             aTTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGt                                                     >  2:3092092/1‑150 (MQ=255)
                                                                                              ttCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGtt                                                    <  2:2883378/150‑1 (MQ=255)
                                                                                                cgcgACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGtttt                                                  <  2:1628215/150‑1 (MQ=255)
                                                                                                   gACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTa                                               <  2:2420827/150‑1 (MQ=255)
                                                                                                   gACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTa                                               <  2:719807/150‑1 (MQ=255)
                                                                                                      cTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGt                                            >  2:942865/1‑150 (MQ=255)
                                                                                                      cTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGt                                            >  1:1390085/1‑150 (MQ=255)
                                                                                                        tctcGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTgg                                          <  2:1184377/150‑1 (MQ=255)
                                                                                                         ctcGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGt                                         >  2:3559473/1‑150 (MQ=255)
                                                                                                            gTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGtttt                                      >  1:2493869/1‑150 (MQ=255)
                                                                                                             ttACTGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTc                                     >  1:1925307/1‑150 (MQ=255)
                                                                                                              tACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTcc                                    <  2:1094564/150‑1 (MQ=255)
                                                                                                                cTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGt                                  <  1:1536463/150‑1 (MQ=255)
                                                                                                                 tGACAGGAAAATGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTc                                 <  2:349039/150‑1 (MQ=255)
                                                                                                                       gAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAAt                           <  2:2382335/150‑1 (MQ=255)
                                                                                                                         aaaTGGGCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAATAttt                         >  1:93543/1‑150 (MQ=255)
                                                                                                                          aaTGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTa                        <  2:4065187/150‑1 (MQ=255)
                                                                                                                            tGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAg                      <  1:388984/150‑1 (MQ=255)
                                                                                                                             gggCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAgg                     <  2:4072352/150‑1 (MQ=255)
                                                                                                                               gCCATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTa                   <  2:3898329/150‑1 (MQ=255)
                                                                                                                               gCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTa                   >  1:3763474/1‑150 (MQ=255)
                                                                                                                                ccATTGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTaa                  >  2:880265/1‑150 (MQ=255)
                                                                                                                                ccATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTaa                  >  1:1242841/1‑150 (MQ=255)
                                                                                                                                 cATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAg                 <  1:3419197/150‑1 (MQ=255)
                                                                                                                                    tGGCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAAc              >  1:363161/1‑150 (MQ=255)
                                                                                                                                     ggCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAAcc             >  1:4010913/1‑150 (MQ=255)
                                                                                                                                      gCAACCAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCt            <  1:3016651/150‑1 (MQ=255)
                                                                                                                                      gCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCt            <  1:4130413/150‑1 (MQ=255)
                                                                                                                                       cAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTg           <  1:1348025/150‑1 (MQ=255)
                                                                                                                                        aaCCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGa          >  2:2539061/1‑150 (MQ=255)
                                                                                                                                        aaCCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGa          >  2:3030084/1‑150 (MQ=255)
                                                                                                                                           cAGGGTAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCt       <  2:2200822/150‑1 (MQ=255)
                                                                                                                                            aGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTc      >  2:2938466/1‑150 (MQ=255)
                                                                                                                                              ggAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGt    <  2:3239333/150‑1 (MQ=255)
                                                                                                                                                aaaGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGa  >  2:2920407/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CCGCGCCGTCGCTGTCGACGCATGCGGATCCTCTAGAGTCGACATATGGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACCTTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGTGGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTCGAAAATTTAAGGTAAGAACCTGACCTCGTGA  >  CP009273/4087332‑4087625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A