Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 608,174 G→A 11.3% intergenic (‑224/‑19) fepA ← / → fes iron‑enterobactin outer membrane transporter/enterobactin/ferric enterobactin esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273608,1740GA11.3% 1042.1 / 92.5 337intergenic (‑224/‑19)fepA/fesiron‑enterobactin outer membrane transporter/enterobactin/ferric enterobactin esterase
Reads supporting (aligned to +/- strand):  ref base G (152/145);  new base A (25/13);  total (179/158)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 340 total aligned reads displayed.

CCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACT  >  CP009273/608025‑608322
                                                                                                                                                     |                                                                                                                                                    
ccGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAg                                                                                                                                                      <  1:2292678/150‑1 (MQ=255)
  gcgAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTa                                                                                                                                                    <  2:247846/150‑1 (MQ=255)
     aaaaCCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAAc                                                                                                                                                 >  2:701068/1‑150 (MQ=255)
       aaCCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACAt                                                                                                                                               <  2:4166339/150‑1 (MQ=255)
         ccTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATgg                                                                                                                                             >  1:5108926/1‑150 (MQ=255)
         ccTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATgg                                                                                                                                             <  2:4488306/150‑1 (MQ=255)
             tGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGtt                                                                                                                                         <  1:2992852/150‑1 (MQ=255)
              gATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGGCCGCGCTTCCCACGGCGCGCCATTACGCGATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTa                                                                                                                                        <  2:2150010/150‑1 (MQ=255)
                tCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATg                                                                                                                                      >  2:4698098/1‑150 (MQ=255)
                     aaaGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGtgtg                                                                                                                                 >  1:3900555/1‑150 (MQ=255)
                      aaGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGa                                                                                                                                <  1:1730209/150‑1 (MQ=255)
                        gACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACg                                                                                                                              >  1:1558255/1‑150 (MQ=255)
                         acaagaagtgcagtTGGTTAATTAATGTCCGCTCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATTTTTATCAATAATATTTTCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACgg                                                                                                                             <  2:3754066/144‑1 (MQ=255)
                             gcagtgcagtTGGTTACTTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGtt                                                                                                                         <  2:4229960/150‑1 (MQ=255)
                             gcagtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGtt                                                                                                                         <  1:1847765/150‑1 (MQ=255)
                             gcagtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGta                                                                                                                         <  2:1045124/150‑2 (MQ=255)
                               agtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTaa                                                                                                                       <  2:5243126/150‑1 (MQ=255)
                                 tgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTaaaa                                                                                                                     <  1:35849/150‑1 (MQ=255)
                                   cagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGt                                                                                                                   >  1:4225032/1‑150 (MQ=255)
                                            aattaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTga                                                                                                          >  2:1708646/1‑150 (MQ=255)
                                             attaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTgag                                                                                                         <  1:3248269/150‑1 (MQ=255)
                                              ttaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTgaga                                                                                                        >  2:3911160/1‑150 (MQ=255)
                                               taatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGagag                                                                                                       <  1:1295548/150‑1 (MQ=255)
                                               taatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGagag                                                                                                       <  1:3911527/150‑1 (MQ=255)
                                                aatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGTAAAAGTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGc                                                                                                      >  2:3832434/1‑150 (MQ=255)
                                                 atGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCt                                                                                                     >  1:5242884/1‑150 (MQ=255)
                                                 atGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCt                                                                                                     >  1:2169155/1‑150 (MQ=255)
                                                 atGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCt                                                                                                     >  1:1338927/1‑150 (MQ=255)
                                                  tGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCtg                                                                                                    >  1:453408/1‑150 (MQ=255)
                                                  tGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCtg                                                                                                    <  2:2907819/150‑1 (MQ=255)
                                                    tCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCtggt                                                                                                  >  2:4058316/1‑150 (MQ=255)
                                                    tCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCtggt                                                                                                  >  2:4837987/1‑150 (MQ=255)
                                                           ttCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTc                                                                                           <  1:1009752/150‑1 (MQ=255)
                                                              ccACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCACTAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGaa                                                                                        <  2:3873855/150‑1 (MQ=255)
                                                              ccACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGaa                                                                                        <  1:4394971/150‑1 (MQ=255)
                                                                 cGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACa                                                                                     <  1:913908/150‑1 (MQ=255)
                                                                  ggCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACAt                                                                                    >  2:2582306/1‑150 (MQ=255)
                                                                  ggCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACAt                                                                                    <  2:1590029/150‑1 (MQ=255)
                                                                  ggCGCGCCATTACGCTATAGCAAATGCAAATAGTTATCAATAATAATATCAATAAATTTCTGCAATCAATGAAAAATTGCACAGTAAAAATGCGGTTATGGTGTGACGACGTTAAAAGTAGGAAGTGAGAGGTGGTGGCAGTCGTAACAt                                                                                    >  2:2761548/1‑150 (MQ=255)
                                                                    cgcgcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATgg                                                                                  >  2:318889/1‑150 (MQ=255)
                                                                    cgcgcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATgg                                                                                  <  2:4951579/150‑1 (MQ=255)
                                                                      cgcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTTCACAGTAAACATGGGGTTATGGTTTGACGGCGTTAAAAGTAGGAAGTGAGATCTGGTGGCAGTCGAAACATGGcc                                                                                >  1:170200/1‑150 (MQ=255)
                                                                      cgcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGATACATGGcc                                                                                >  2:823570/1‑150 (MQ=255)
                                                                       gcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGccc                                                                               >  2:204506/1‑150 (MQ=255)
                                                                        ccATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCg                                                                              >  1:445238/1‑150 (MQ=255)
                                                                          aTTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGa                                                                            >  1:2919656/1‑150 (MQ=255)
                                                                          aTTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGa                                                                            <  1:4159831/150‑1 (MQ=255)
                                                                           ttACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGaa                                                                           <  1:4059552/150‑1 (MQ=255)
                                                                            tACGCTACTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAAt                                                                          <  1:4479644/150‑1 (MQ=255)
                                                                              cGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATgg                                                                        >  1:5045469/1‑150 (MQ=255)
                                                                              cGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATgg                                                                        >  1:3744431/1‑150 (MQ=255)
                                                                                cTATTGCACATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGTTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCa                                                                      >  2:2431435/1‑150 (MQ=255)
                                                                                cTATTGCAAATGCAAATAGTTATCAATAATAATATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGTCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCa                                                                      >  1:2046004/1‑150 (MQ=255)
                                                                                     gcaaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTc                                                                 >  1:1095840/1‑150 (MQ=255)
                                                                                     gcaaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTc                                                                 <  1:800181/150‑1 (MQ=255)
                                                                                           gcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATg                                                           >  1:581999/1‑150 (MQ=255)
                                                                                           gaaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATg                                                           <  2:1195493/148‑1 (MQ=255)
                                                                                            caaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGa                                                          <  2:360600/150‑1 (MQ=255)
                                                                                            caaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGa                                                          >  2:1139522/1‑150 (MQ=255)
                                                                                              aatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGATTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACg                                                        >  1:668597/1‑150 (MQ=255)
                                                                                                 agatATCAATAATAATATCAAAAAATTTCAGCAATCAAAGAAAAATTGCACAGTAAACATGCGGATATGGAGTGACCGCGTTAAAAGAAGGAAGTGAGAGCTGGTGGCAGACGAAACAAGGCCCGGAATGGAAGCTACTGAATGActaca                                                     >  2:1995732/4‑146 (MQ=255)
                                                                                                 aGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGACTGGCAGCGTCTGAATGACGaaa                                                     >  1:4847273/1‑150 (MQ=255)
                                                                                                 aGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGaaa                                                     >  2:1563692/1‑150 (MQ=255)
                                                                                                   ttATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATg                                                   >  1:1206891/1‑150 (MQ=255)
                                                                                                    taacAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGt                                                  <  1:2676454/147‑1 (MQ=255)
                                                                                                     aTCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGtt                                                 >  1:276204/1‑150 (MQ=255)
                                                                                                       cAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTg                                               >  1:3501253/1‑150 (MQ=255)
                                                                                                          taataTTATCAATATATTTATGCAATCAATGAAAAATTTCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGATACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAgg                                            <  2:4391678/150‑1 (MQ=255)
                                                                                                           aataTTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGt                                           >  1:2618216/1‑150 (MQ=255)
                                                                                                                  tCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTc                                    <  1:3389308/150‑1 (MQ=255)
                                                                                                                   cAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCt                                   <  1:1605904/150‑1 (MQ=255)
                                                                                                                     atatatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCTTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCACCGTCTGAATGAAGAAATGTTTGAGGTAACTTTCtgt                                 >  1:5093348/1‑149 (MQ=255)
                                                                                                                      tatatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCtggt                                >  2:1869688/1‑150 (MQ=255)
                                                                                                                       atatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCtggtg                               >  1:1443088/1‑150 (MQ=255)
                                                                                                                       atatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCtggtg                               >  1:4645958/1‑150 (MQ=255)
                                                                                                                        tatTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCtggtgg                              <  2:4582435/150‑1 (MQ=255)
                                                                                                                         atTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGc                             >  1:3038883/1‑150 (MQ=255)
                                                                                                                         atTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGc                             >  1:402003/1‑150 (MQ=255)
                                                                                                                          tttCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCg                            >  2:1611768/1‑150 (MQ=255)
                                                                                                                           ttCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGt                           >  2:4513899/1‑150 (MQ=255)
                                                                                                                            tCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCTTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTg                          <  2:1796471/150‑1 (MQ=255)
                                                                                                                            tCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTg                          <  1:3470731/150‑1 (MQ=255)
                                                                                                                             cTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGa                         >  1:1991059/1‑150 (MQ=255)
                                                                                                                                    caatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCaa                  >  2:2624332/1‑150 (MQ=255)
                                                                                                                                     aatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAg                 >  1:4278588/1‑150 (MQ=255)
                                                                                                                                     aatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAg                 >  2:1097892/1‑150 (MQ=255)
                                                                                                                                     aatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAg                 >  2:1973883/1‑150 (MQ=255)
                                                                                                                                      atGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAAGGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAgg                <  2:3076360/150‑1 (MQ=255)
                                                                                                                                       tGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGt               >  2:1952180/1‑150 (MQ=255)
                                                                                                                                        gAAAAATTTCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              <  2:3983356/150‑1 (MQ=255)
                                                                                                                                        gAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              <  2:5085361/150‑1 (MQ=255)
                                                                                                                                        gAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              <  1:4984266/150‑1 (MQ=255)
                                                                                                                                         aaaaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGACTGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTc             >  2:3420261/1‑150 (MQ=255)
                                                                                                                                         aaaaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTc             >  1:3322527/1‑150 (MQ=255)
                                                                                                                                         aaaaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTc             <  1:3618378/150‑1 (MQ=255)
                                                                                                                                           aaaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTg           >  2:2078542/1‑150 (MQ=255)
                                                                                                                                            aaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTga          <  1:1403339/150‑1 (MQ=255)
                                                                                                                                            aaTTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTga          <  1:23867/150‑1 (MQ=255)
                                                                                                                                               tGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTgaaga       <  1:2434877/150‑1 (MQ=255)
                                                                                                                                                  acaGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATa    >  1:1306993/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACT  >  CP009273/608025‑608322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A