Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 608,174 G→A 20.4% intergenic (‑224/‑19) fepA ← / → fes iron‑enterobactin outer membrane transporter/enterobactin/ferric enterobactin esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273608,1740GA20.4% 852.3 / 204.6 358intergenic (‑224/‑19)fepA/fesiron‑enterobactin outer membrane transporter/enterobactin/ferric enterobactin esterase
Reads supporting (aligned to +/- strand):  ref base G (154/129);  new base A (36/37);  total (191/167)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

CCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACT  >  CP009273/608025‑608322
                                                                                                                                                     |                                                                                                                                                    
   cgAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTaa                                                                                                                                                   <  2:1680194/150‑1 (MQ=255)
    gAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTaaa                                                                                                                                                  >  1:1023930/1‑150 (MQ=255)
      aaaCCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACa                                                                                                                                                <  2:2870063/150‑1 (MQ=255)
        aCCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATg                                                                                                                                              >  1:3107132/1‑150 (MQ=255)
          cTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATggg                                                                                                                                            <  1:5394960/150‑1 (MQ=255)
           tttGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATgggg                                                                                                                                           >  2:1892829/1‑150 (MQ=255)
            ttGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGt                                                                                                                                          >  2:42889/1‑150 (MQ=255)
               aTCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTAt                                                                                                                                       <  1:3778514/150‑1 (MQ=255)
                tCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATg                                                                                                                                      >  2:3047916/1‑150 (MQ=255)
                  cTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGTCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGgt                                                                                                                                    <  2:3493466/150‑1 (MQ=255)
                  cTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGgt                                                                                                                                    >  2:2130181/1‑150 (MQ=255)
                    gAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGgtgt                                                                                                                                  <  2:1430163/150‑1 (MQ=255)
                    gAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCGTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGgtgt                                                                                                                                  <  1:4930150/150‑1 (MQ=255)
                      aaGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGa                                                                                                                                <  2:696710/150‑1 (MQ=255)
                           acGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCg                                                                                                                           <  2:846124/150‑1 (MQ=255)
                            cGCAGTGCAGTTGGTTAATAAATGTCCGCGCTTCCCACGGCGCGCCATTACTCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTTCACAATAAACATGGTGTTATGGTGTGACGGCGt                                                                                                                          >  2:1381873/1‑150 (MQ=255)
                             gcagtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGtt                                                                                                                         >  1:705623/1‑150 (MQ=255)
                             gcagtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGtt                                                                                                                         <  2:5169874/150‑1 (MQ=255)
                               agtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTaa                                                                                                                       >  1:5666710/1‑150 (MQ=255)
                                  gcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAg                                                                                                                    <  2:4630089/150‑1 (MQ=255)
                                   cagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGt                                                                                                                   >  1:2633879/1‑150 (MQ=255)
                                    agtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTa                                                                                                                  >  1:4012383/1‑150 (MQ=255)
                                     gtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAg                                                                                                                 >  2:5272492/1‑150 (MQ=255)
                                      ttGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAgg                                                                                                                >  2:1943452/1‑150 (MQ=255)
                                           taattaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTg                                                                                                           >  2:256994/1‑150 (MQ=255)
                                            aattaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTga                                                                                                          <  2:5629462/150‑1 (MQ=255)
                                            aattaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTga                                                                                                          <  2:4580750/150‑1 (MQ=255)
                                            aattaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTga                                                                                                          >  1:4138726/1‑150 (MQ=255)
                                             attaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTgag                                                                                                         <  2:2444297/150‑1 (MQ=255)
                                              ttaatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTgaga                                                                                                        <  2:2201839/150‑1 (MQ=255)
                                                aatGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGc                                                                                                      <  1:2968685/150‑1 (MQ=255)
                                                 atGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCt                                                                                                     <  1:2541225/150‑1 (MQ=255)
                                                 atGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCt                                                                                                     <  2:4810313/150‑1 (MQ=255)
                                                  tGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCtg                                                                                                    >  1:2808823/1‑150 (MQ=255)
                                                         gcTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGAAg                                                                                             >  1:3570754/1‑150 (MQ=255)
                                                          cTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGt                                                                                            <  1:1622273/150‑1 (MQ=255)
                                                              ccACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGaa                                                                                        >  2:1327593/1‑150 (MQ=255)
                                                              ccACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGaa                                                                                        <  2:392730/150‑1 (MQ=255)
                                                               cACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGaaa                                                                                       >  1:5028617/1‑150 (MQ=255)
                                                                aCGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAAc                                                                                      >  1:1098777/1‑150 (MQ=255)
                                                                  ggCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACAt                                                                                    >  1:1203043/1‑150 (MQ=255)
                                                                     gcgcCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGc                                                                                 <  1:5237813/150‑1 (MQ=255)
                                                                         cATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGTAAGTGAGAGATGGTGGCAGTCGAAACATggagcgg                                                                             >  2:3417313/1‑145 (MQ=255)
                                                                            tACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAAt                                                                          >  2:5070231/1‑150 (MQ=255)
                                                                             aCGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATg                                                                         >  1:568071/1‑150 (MQ=255)
                                                                             aCGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATg                                                                         >  1:3421958/1‑150 (MQ=255)
                                                                             aCGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATg                                                                         >  2:1754257/1‑150 (MQ=255)
                                                                               gCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGc                                                                       >  2:2756448/1‑150 (MQ=255)
                                                                                cTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCa                                                                      >  2:1040996/1‑150 (MQ=255)
                                                                                cTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCa                                                                      >  2:3884980/1‑150 (MQ=255)
                                                                                    tgcaaatgcaaaTAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGt                                                                  <  1:190929/150‑1 (MQ=255)
                                                                                     gcaaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTc                                                                 >  1:4610511/1‑150 (MQ=255)
                                                                                      caaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACAGGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCt                                                                >  2:5380342/1‑150 (MQ=255)
                                                                                      caaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCt                                                                >  2:120345/1‑150 (MQ=255)
                                                                                       aaatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTg                                                               >  1:2846974/1‑150 (MQ=255)
                                                                                        aatgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGa                                                              >  2:3688907/1‑150 (MQ=255)
                                                                                          tgcaaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAAt                                                            <  1:3281540/150‑1 (MQ=255)
                                                                                            caaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTCAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGa                                                          >  2:3602978/1‑150 (MQ=255)
                                                                                            caaatAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGa                                                          >  2:3254685/1‑150 (MQ=255)
                                                                                                tAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAAAGGCAGCGTCTGAATGACGaa                                                      >  2:4416167/1‑150 (MQ=255)
                                                                                                tAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGaa                                                      <  2:1843120/150‑1 (MQ=255)
                                                                                                tAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGAGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGaa                                                      >  2:1025544/1‑150 (MQ=255)
                                                                                                 aGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGaaa                                                     >  1:4639260/1‑150 (MQ=255)
                                                                                                      tCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGttt                                                <  1:3047916/150‑1 (MQ=255)
                                                                                                      tCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGttt                                                <  1:4870437/150‑1 (MQ=255)
                                                                                                       cAATAATATTATCAATAGATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTg                                               <  2:5231110/150‑1 (MQ=255)
                                                                                                           aataTTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGt                                           <  2:2516055/150‑1 (MQ=255)
                                                                                                           aataTTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGt                                           >  2:1450333/1‑150 (MQ=255)
                                                                                                            atatTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTc                                          <  1:1531647/150‑1 (MQ=255)
                                                                                                              attatCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCAc                                        >  2:4869665/1‑150 (MQ=255)
                                                                                                               ttatCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACt                                       <  1:5645584/150‑1 (MQ=255)
                                                                                                               ttatCAATATATTTCTGCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACt                                       <  1:4408018/150‑1 (MQ=255)
                                                                                                                       atatTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCtggtg                               <  2:2590962/150‑1 (MQ=255)
                                                                                                                           ttCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGt                           <  1:1673229/150‑1 (MQ=255)
                                                                                                                             cTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGa                         >  1:2478584/1‑150 (MQ=255)
                                                                                                                              tGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGAt                        <  2:1086519/150‑1 (MQ=255)
                                                                                                                               gCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATc                       <  2:1073726/150‑1 (MQ=255)
                                                                                                                               gCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATc                       <  1:1753977/150‑1 (MQ=255)
                                                                                                                               gCAATCAATGAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATc                       <  1:3714781/150‑1 (MQ=255)
                                                                                                                                 aatcaatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATccc                     >  1:3331094/1‑150 (MQ=255)
                                                                                                                                    caatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCaa                  <  1:1773067/150‑1 (MQ=255)
                                                                                                                                    caatGAAAAATTGCACAGTAAACAAGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGATAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTACTGGTGGCGTGATCCCCaa                  >  2:3726781/1‑150 (MQ=255)
                                                                                                                                     aatGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAg                 >  1:1346780/1‑150 (MQ=255)
                                                                                                                                      atGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAgg                <  2:273229/150‑1 (MQ=255)
                                                                                                                                       tGAAAATTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGACTGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGt               >  2:4072626/1‑150 (MQ=255)
                                                                                                                                       tGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAACTGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGt               >  2:3163361/1‑150 (MQ=255)
                                                                                                                                        gAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              >  1:2393472/1‑150 (MQ=255)
                                                                                                                                        gAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              >  2:4528738/1‑150 (MQ=255)
                                                                                                                                        gAAAAATTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGtt              <  2:4481718/150‑1 (MQ=255)
                                                                                                                                          aaaaTTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCt            <  2:3107132/150‑1 (MQ=255)
                                                                                                                                          aaaaTTGCACAATAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCt            <  1:412140/150‑1 (MQ=255)
                                                                                                                                            aaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTga          >  1:2104732/1‑150 (MQ=255)
                                                                                                                                            aaTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTga          >  1:5573668/1‑150 (MQ=255)
                                                                                                                                             aTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTgaa         >  1:872997/1‑150 (MQ=255)
                                                                                                                                             aTTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTgaa         <  2:2426217/150‑1 (MQ=255)
                                                                                                                                               tGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTgaaga       <  1:3942506/150‑1 (MQ=255)
                                                                                                                                                   caGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATAc   >  2:1749734/1‑150 (MQ=255)
                                                                                                                                                    aGTAAACCTGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGGGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACt  <  2:3988232/150‑1 (MQ=255)
                                                                                                                                                    aGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACt  <  1:2257769/150‑1 (MQ=255)
                                                                                                                                                    aGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACt  >  1:306928/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGCGAAAACCTTTGATCCTGAAAGACACGCAGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATTTCTGCAATCAATGAAAAATTGCACAGTAAACATGGGGTTATGGTGTGACGGCGTTAAAAGTAGGAAGTGAGAGCTGGTGGCAGTCGAAACATGGCCCGGAATGGCAGCGTCTGAATGACGAAATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACT  >  CP009273/608025‑608322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A