Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 632,406 T→A 36.2% K223N (AAA→AAT)  ybdO ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273632,4060TA36.2% 361.4 / inf 343K223N (AAA→AAT) ybdOputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (110/108);  new base A (59/65);  total (169/174)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 348 total aligned reads displayed.

TTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGT  >  CP009273/632258‑632555
                                                                                                                                                    |                                                                                                                                                     
ttGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                                                                                                                                                      <  2:2170800/150‑1 (MQ=255)
   aaGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAAAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGc                                                                                                                                                   >  1:3862290/1‑150 (MQ=255)
    atgTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCg                                                                                                                                                  <  2:1741943/148‑1 (MQ=255)
      gTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAt                                                                                                                                                >  1:4046938/1‑150 (MQ=255)
      gTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAt                                                                                                                                                >  2:3479847/1‑150 (MQ=255)
      gTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAt                                                                                                                                                >  1:3036636/1‑150 (MQ=255)
         aCGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCa                                                                                                                                             >  2:2121770/1‑150 (MQ=255)
           ggATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATa                                                                                                                                           >  2:4883807/1‑150 (MQ=255)
            gATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATaa                                                                                                                                          <  1:3938169/150‑1 (MQ=255)
             aTGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATaaa                                                                                                                                         <  1:4510349/150‑1 (MQ=255)
             aTGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATaaa                                                                                                                                         <  1:2619238/150‑1 (MQ=255)
              tGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGACTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAt                                                                                                                                        >  1:3241109/1‑150 (MQ=255)
              tGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAAt                                                                                                                                        <  1:4742741/150‑1 (MQ=255)
                  cTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAAtctct                                                                                                                                    >  2:1018324/1‑150 (MQ=255)
                    aGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCg                                                                                                                                  <  2:1782486/150‑1 (MQ=255)
                      ttttATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTc                                                                                                                                >  2:322654/1‑150 (MQ=255)
                      ttttATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTc                                                                                                                                >  2:4494056/1‑150 (MQ=255)
                         tATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTAt                                                                                                                             <  1:2140399/150‑1 (MQ=255)
                          aTCTATTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAtt                                                                                                                            <  2:1487239/150‑1 (MQ=255)
                            ctcgcttaagttaagAAAGTCACGGTAGGAATTATAAAGGTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTc                                                                                                                          <  1:3986451/145‑1 (MQ=255)
                              ctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCa                                                                                                                        >  2:1884783/1‑150 (MQ=255)
                              ctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCa                                                                                                                        >  1:857145/1‑150 (MQ=255)
                              ctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCa                                                                                                                        >  1:1339025/1‑150 (MQ=255)
                                tttAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCAtt                                                                                                                      >  1:1881883/1‑150 (MQ=255)
                                  taagttaagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTg                                                                                                                    >  2:4744283/1‑150 (MQ=255)
                                     gttaagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAat                                                                                                                 <  1:10238/150‑1 (MQ=255)
                                      ttaagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAata                                                                                                                <  2:3255581/150‑1 (MQ=255)
                                       taagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAatat                                                                                                               <  1:4460012/150‑1 (MQ=255)
                                        aagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATatc                                                                                                              <  1:327864/150‑1 (MQ=255)
                                           aaaGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATatcatc                                                                                                           >  1:1127592/1‑150 (MQ=255)
                                           aaaGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATatcatc                                                                                                           <  1:3118209/150‑1 (MQ=255)
                                            aaGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATAtcatca                                                                                                          <  2:847667/150‑1 (MQ=255)
                                              gTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAc                                                                                                        <  1:3181023/150‑1 (MQ=255)
                                                   ggTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCctg                                                                                                   <  1:1365851/150‑1 (MQ=255)
                                                    gTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCctga                                                                                                  >  1:625971/1‑149 (MQ=255)
                                                       ggAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGa                                                                                               >  1:125357/1‑150 (MQ=255)
                                                       ggAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGa                                                                                               >  1:921734/1‑150 (MQ=255)
                                                        gAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGAt                                                                                              <  1:769652/150‑1 (MQ=255)
                                                         aaTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGAtt                                                                                             >  2:3106734/1‑150 (MQ=255)
                                                         aaTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGAtt                                                                                             >  2:1631082/1‑150 (MQ=255)
                                                         aaTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACAGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCATGCTGAtt                                                                                             >  2:4384025/1‑150 (MQ=255)
                                                           ttATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTa                                                                                           <  2:4212711/150‑1 (MQ=255)
                                                             ataAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGa                                                                                         >  2:330839/1‑150 (MQ=255)
                                                             ataAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGa                                                                                         <  2:3436458/150‑1 (MQ=255)
                                                                aaGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGatat                                                                                      <  2:4961031/150‑1 (MQ=255)
                                                                 aGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATAtt                                                                                     >  1:241788/1‑150 (MQ=255)
                                                                    tCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAAt                                                                                  >  1:2968408/1‑150 (MQ=255)
                                                                      aTAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTg                                                                                >  2:1987458/1‑150 (MQ=255)
                                                                       tAGGGGACTATTCCCAGTAAATCTGTAACTGCACTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTgg                                                                               <  2:314594/150‑1 (MQ=255)
                                                                        aGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTggg                                                                              <  1:4312614/150‑1 (MQ=255)
                                                                         ggggACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATACATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGt                                                                             >  1:1794094/1‑150 (MQ=255)
                                                                             aCTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAAc                                                                         <  1:694381/150‑1 (MQ=255)
                                                                             aCTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAAc                                                                         <  2:1165379/150‑1 (MQ=255)
                                                                              cTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAAct                                                                        <  1:3574360/150‑1 (MQ=255)
                                                                               tATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAActc                                                                       <  2:1107367/150‑1 (MQ=255)
                                                                               tATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAActc                                                                       <  1:519169/150‑1 (MQ=255)
                                                                                 ttCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACtctt                                                                     >  2:1371120/1‑150 (MQ=255)
                                                                                   cccAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACtcttct                                                                   >  1:3791894/1‑150 (MQ=255)
                                                                                      aGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGcc                                                                <  2:1359284/150‑1 (MQ=255)
                                                                                        tAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCAt                                                              >  2:2603225/1‑150 (MQ=255)
                                                                                           aTCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAAt                                                           >  2:3082959/1‑150 (MQ=255)
                                                                                            tCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAAtt                                                          <  1:1552210/150‑1 (MQ=255)
                                                                                             cTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAAttt                                                         <  2:3546284/150‑1 (MQ=255)
                                                                                                 aaCTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTc                                                     <  1:301481/150‑1 (MQ=255)
                                                                                                  aCTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCa                                                    <  1:3691832/150‑1 (MQ=255)
                                                                                                    tGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATa                                                  >  2:720668/1‑150 (MQ=255)
                                                                                                      cAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAg                                                >  1:4780838/1‑150 (MQ=255)
                                                                                                       aaTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGt                                               >  1:1623554/1‑150 (MQ=255)
                                                                                                        aTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTg                                              <  1:3501605/150‑1 (MQ=255)
                                                                                                         tGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGc                                             <  2:2368661/150‑1 (MQ=255)
                                                                                                          gCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCt                                            <  1:2014238/150‑1 (MQ=255)
                                                                                                            tATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTAt                                          <  2:921734/150‑1 (MQ=255)
                                                                                                             aTTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTAtt                                         >  1:4219323/1‑150 (MQ=255)
                                                                                                              ttAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTg                                        <  2:3501426/150‑1 (MQ=255)
                                                                                                               tAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATAGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGt                                       >  2:4333640/1‑150 (MQ=255)
                                                                                                                aaTGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATATGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTc                                      >  2:2855160/1‑150 (MQ=255)
                                                                                                                aaTGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTc                                      <  2:5070499/150‑1 (MQ=255)
                                                                                                                       gTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAAtt                               <  1:2831017/150‑1 (MQ=255)
                                                                                                                         cAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCg                             <  2:4046938/150‑1 (MQ=255)
                                                                                                                          aGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAACTCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGt                            <  2:1645040/150‑1 (MQ=255)
                                                                                                                           gCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTg                           <  2:2651355/150‑1 (MQ=255)
                                                                                                                            cAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTgg                          >  2:2236557/1‑150 (MQ=255)
                                                                                                                             aaaGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGa                         <  1:1924451/150‑1 (MQ=255)
                                                                                                                               agagGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATg                       <  2:3911082/150‑1 (MQ=255)
                                                                                                                                 agGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGcc                     >  2:2967986/1‑150 (MQ=255)
                                                                                                                                 agGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGGCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGcc                     >  1:1699867/1‑150 (MQ=255)
                                                                                                                                  ggAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCt                    <  2:659370/150‑1 (MQ=255)
                                                                                                                                   gAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTa                   <  1:4062391/150‑1 (MQ=255)
                                                                                                                                    aaCCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTAt                  >  2:2038770/1‑150 (MQ=255)
                                                                                                                                        aCGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATtact              <  1:225492/150‑1 (MQ=255)
                                                                                                                                         cGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATtacta             >  1:4400208/1‑150 (MQ=255)
                                                                                                                                           aaaTGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATtactagc           >  1:3432280/1‑148 (MQ=255)
                                                                                                                                             aTGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACaaa         <  1:4693842/150‑1 (MQ=255)
                                                                                                                                               gagaTATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAAtt       >  1:2770793/1‑150 (MQ=255)
                                                                                                                                                agaTATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTa      >  1:2517018/1‑150 (MQ=255)
                                                                                                                                                 gaTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTag     <  2:3521633/150‑1 (MQ=255)
                                                                                                                                                 gaTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTag     >  2:2511535/1‑150 (MQ=255)
                                                                                                                                                  aTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTaga    >  1:4924158/1‑150 (MQ=255)
                                                                                                                                                    tttGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGt  <  2:1330286/150‑1 (MQ=255)
                                                                                                                                                    tttGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGt  >  1:5079889/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGT  >  CP009273/632258‑632555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A